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    • 2. 发明授权
    • Method and apparatus for determining the concentration of a substance in a liquid
    • 用于确定液体中物质浓度的方法和装置
    • US08045172B2
    • 2011-10-25
    • US12487912
    • 2009-06-19
    • Rafael Egger
    • Rafael Egger
    • G01N21/55
    • G01N21/8483G01N21/314
    • In a method for determining the concentration of at least one substance in a liquid the liquid is applied onto a test strip containing at least one test zone, wherein an optical sensor arrangement is moved step-by-step in a first direction over the surface of the test strip while the test trip is at the same time irradiated with light of a predetermined wave length and wherein in each step the radiation reflected from the surface of the test strip is measured, and wherein in each measurement step the test strip surface is irradiated alternately with light of at least two different wave lengths and the irradiation is measured at the same time and the difference between the measurement signals obtained in each measurement step using irradiation light of different wave lengths is analyzed.
    • 在用于确定液体中至少一种物质的浓度的方法中,将液体施加到含有至少一个测试区域的测试条上,其中光学传感器装置在第一方向上逐步移动到 测试条同时用预定波长的光照射,并且其中在每个步骤中测量从测试条表面反射的辐射,并且其中在每个测量步骤中辐射测试条表面 交替地具有至少两个不同波长的光,并且同时测量照射,并且分析使用不同波长的照射光在每个测量步骤中获得的测量信号之间的差。
    • 3. 发明申请
    • METHOD FOR THE QUANTITATIVE DETERMINATION OF THE CONCENTRATION OF FLUOROPHORES OF A SUBSTANCE IN A SAMPLE AND APPARATUS FOR CARRYING OUT THE SAME
    • 用于定量确定样品中物质的荧光浓度的方法及其实施方法
    • US20100117003A1
    • 2010-05-13
    • US12616811
    • 2009-11-12
    • Rafael Egger
    • Rafael Egger
    • G01J1/58
    • G01N21/278G01N21/6428
    • The invention concerns a method and an apparatus for the quantitative determination of the concentration of fluorophores of at least one substance in a sample. A constant portion of the reference light of a reference light wave length (λr) emitted by a reference light source (28) is coupled in by an optical element (REM) in the direction of a receiving element (38). A first measured value is detected which corresponds to the portion of the reference light coupled in which is incident on the receiving element (38). The sample is irradiated with the excitation light of an excitation wave length (λex) emitted by an excitation light source (26). A second measured valued is detected which corresponds to the portion of the fluorescent light of an emission wave length (λem) emitted by the sample which is incident on the receiving element (38). The ratio (Emes2/Es2) of the second measured value (Emes2) to the first measured value (Es2) is determined. The number of fluorophores in the substance of the sample within the detection zone is determined taking into account the determined ratio (Emes2/Es2).
    • 本发明涉及用于定量测定样品中至少一种物质的荧光团浓度的方法和装置。 由参考光源(28)发射的参考光波长(λr)的参考光的恒定部分通过光学元件(REM)在接收元件(38)的方向上耦合。 检测到与其中入射在接收元件(38)上的参考光的部分对应的第一测量值。 用激发光源(26)发射的激发波长(λex)的激发光照射样品。 检测第二测量值,其对应于入射在接收元件(38)上的样品发射的发射波长(λem)的荧光的部分。 确定第二测量值(Emes2)与第一测量值(Es2)的比(Emes2 / Es2)。 在检测区内样品物质中的荧光团数目是考虑到确定的比例(Emes2 / Es2)来确定的。
    • 4. 发明授权
    • Method for the quantitative determination of the concentration of fluorophores of a substance in a sample and apparatus for carrying out the same
    • 用于定量测定样品中物质的荧光团的浓度的方法和用于进行该物质的装置的方法
    • US08334522B2
    • 2012-12-18
    • US12616811
    • 2009-11-12
    • Rafael Egger
    • Rafael Egger
    • F21V9/16G01J1/58G01T1/10G21H3/02G21K5/00H01J65/06H01J65/08
    • G01N21/278G01N21/6428
    • The invention concerns methods and apparatuses for quantitatively determining the concentration of fluorophores of a substance in a sample. A constant portion of the reference light of a reference light wave length (λr) emitted by a reference light source is coupled in by an optical element in the direction of a receiving element. A first value corresponding to the portion of the reference light coupled in which is incident on the receiving element is detected. The sample is irradiated with the excitation light of an excitation wave length (λex) emitted by an excitation light source. A second value corresponding to the portion of the fluorescent light of an emission wave length (λem) emitted by the sample which is incident on the receiving element. The ratio of the second value to the first value is determined. The number of fluorophores in the substance is determined based on the ratio.
    • 本发明涉及用于定量测定样品中物质的荧光团浓度的方法和装置。 由参考光源发射的参考光波长(λr)的参考光的恒定部分通过光学元件沿接收元件的方向耦合。 检测对应于其中入射在接收元件上的参考光的部分的第一值。 用激发光源发射的激发波长(λex)的激发光照射样品。 对应于由入射在接收元件上的样品发射的发射波长(λem)的荧光的部分的第二值。 确定第二值与第一值的比率。 该物质中荧光团的数量是根据比例来确定的。
    • 7. 发明申请
    • DEVICE AND METHOD FOR DETERMINING THE CONCENTRATION OF FLUOROPHORES IN A SAMPLE
    • 用于测定样品中荧光素浓度的装置和方法
    • US20120261591A1
    • 2012-10-18
    • US13444027
    • 2012-04-11
    • Rafael EggerJörg Zeiner
    • Rafael EggerJörg Zeiner
    • G01N21/64
    • G01N21/645G01N21/278G01N2201/06113G01N2201/062G01N2201/068
    • A device (10) and a method for analyzing a sample (16) containing fluorophores use a light source (12) emitting light (λex) onto the sample (16), and onto a fluorescence standard (14). The fluorophores of the sample (16), given an immission of light of a first wavelength (λex1), have a first excitation efficiency and, given an immission of light of a second wavelength (λex2), have a second excitation efficiency. The fluorescence standard (14), given the same immissions of light, has a third excitation efficiency and, a fourth excitation efficiency. An optical element (20) which is arranged between the light source (12) and the sample (16) and/or (12) the fluorescence standard (14) adapts, due to its optical property, a first difference between the first excitation efficiency and the second excitation efficiency and a second difference between the third excitation efficiency and the fourth excitation efficiency to each other.
    • 用于分析含有荧光团的样品(16)的装置(10)和方法使用在样品(16)上发射光(λex)的光源(12)和荧光标准品(14)。 给定发射第一波长(λex1)的光的样品(16)的荧光团具有第一激发效率,并且在给出第二波长(λex2)的光的情况下,具有第二激发效率。 给予相同光量的荧光标准(14)具有第三激发效率和第四激发效率。 布置在光源(12)和样品(16)和/或(12)荧光标准品(14)之间的光学元件(20)由于其光学特性而适应第一激发效率 第二激励效率和第三励磁效率与第四励磁效率之间的第二差。
    • 10. 发明申请
    • METHOD AND APPARATUS FOR DETERMINING THE CONCENTRATION OF A SUBSTANCE IN A LIQUID
    • 用于测定液体中物质浓度的方法和装置
    • US20090323070A1
    • 2009-12-31
    • US12487912
    • 2009-06-19
    • Rafael Egger
    • Rafael Egger
    • G01N21/55
    • G01N21/8483G01N21/314
    • In a method for determining the concentration of at least one substance in a liquid the liquid is applied onto a test strip containing at least one test zone, wherein an optical sensor arrangement is moved step-by-step in a first direction over the surface of the test strip while the test trip is at the same time irradiated with light of a predetermined wave length and wherein in each step the radiation reflected from the surface of the test strip is measured, and wherein in each measurement step the test strip surface is irradiated alternately with light of at least two different wave lengths and the irradiation is measured at the same time and the difference between the measurement signals obtained in each measurement step using irradiation light of different wave lengths is analyzed.
    • 在用于确定液体中至少一种物质的浓度的方法中,将液体施加到含有至少一个测试区域的测试条上,其中光学传感器装置在第一方向上逐步移动到 测试条同时用预定波长的光照射,并且其中在每个步骤中测量从测试条表面反射的辐射,并且其中在每个测量步骤中辐射测试条表面 交替地具有至少两个不同波长的光,并且同时测量照射,并且分析使用不同波长的照射光在每个测量步骤中获得的测量信号之间的差。