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1. 发明授权

US06939849B1 Recombinant PDGF A-chain homodimers and methods of use 失效
标题翻译：重组PDGF A链同源二聚体及其使用方法
公开(公告)号：US06939849B1
公开(公告)日：2005-09-06
申请号：US08453350
申请日：1995-05-30
申请人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
发明人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
IPC分类号： C07K14/49 , C12N9/02 , C12N15/62 , C12N15/81 , C12N15/85 , A61K38/18 , C12N1/21 , C12N15/18 , C12N15/63
CPC分类号： C12N9/0089 , C07K14/49 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N15/62 , C12N15/81 , C12N15/85 , C12N2800/108 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/36 , C12N2830/55 , C12N2830/702
摘要： DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
摘要翻译：编码两种形式的PDGF A链多肽的DNA，构建用于在酵母和哺乳动物细胞中表达这种DNA的表达载体，以及在酵母和哺乳动物细胞中表达这种DNA以产生活性PDGF A链同源二聚体和活性PDGFA- 链/ B链异源二聚体。

2. 发明授权

US5326695A Platelet derived growth factor agonists 失效
标题翻译：血小板衍生生长因子激动剂
公开(公告)号：US5326695A
公开(公告)日：1994-07-05
申请号：US883949
申请日：1992-05-15
申请人： Maria Andersson , Gudrun Backstrom , Ulla Engstrom , Carl-Henrik Heldin , Ulf Hellman , Arne stman , Bengt Westermark
发明人： Maria Andersson , Gudrun Backstrom , Ulla Engstrom , Carl-Henrik Heldin , Ulf Hellman , Arne stman , Bengt Westermark
IPC分类号： A61K38/00 , C07K14/49 , C12N5/00 , C12N15/00 , C12P21/00
CPC分类号： C07K14/49 , A61K38/00
摘要： The invention described PDGF modifiers. These are peptides which are derived from wild type PDGF monomers, and function as either agonists or antagonists. Various uses of these are described, as well as recombinant means of production. PDGF-B agonists comprising amino acids 97-180 of the PDGF-B monomer having the cysteines at amino acid residues 124 and 133 substituted, nucleic acid molecules encoding the agonists, plasmids, transformed host cells, and methods for causing receptor dimerization and autophosphorylation in a cell having PDGF-.beta. receptors on its surface comprising administering the PDGF-B agonist form part of the invention.
摘要翻译：本发明描述了PDGF改性剂。这些是衍生自野生型PDGF单体的肽，并且作为激动剂或拮抗剂起作用。描述了这些的各种用途，以及重组生产手段。包含在氨基酸残基124和133处具有半胱氨酸的PDGF-B单体的氨基酸97-180的PDGF-B激动剂被取代，编码激动剂，质粒，转化的宿主细胞的核酸分子和用于引发受体二聚化和自磷酸化的方法在其表面上具有PDGF-β受体的细胞包括施用形成本发明一部分的PDGF-B激动剂。

3. 发明授权

US5198357A Preparation of a blood platelet lysate for use in a cell culture medium for hybridoma cells 失效
标题翻译：用于混合细胞的细胞培养基中的血液平板液的制备
公开(公告)号：US5198357A
公开(公告)日：1993-03-30
申请号：US634113
申请日：1990-12-26
申请人： Olov Holmovist , Bengt Westermark
发明人： Olov Holmovist , Bengt Westermark
IPC分类号： C12N5/10 , C12N5/16 , C12R1/91
CPC分类号： C12N5/163 , C12N2500/84 , C12N2500/90
摘要： A platelet lysate is produced from plasma derived from animal whole blood that contains an added citrate to prevent coagulation of the blood during storage. The lysate is prepared by centrifuging plasma to produce a platelet rich paste, adding calcium to the paste to lyse the platelets therein and coagulate fibrinogen to produce a clear liquid containing lysed platelets, sterile filtering the liquid and collecting a liquid filtrate containing the lysed platelets. The obtained lysate of platelets can be used to wholly or partly replace foetal calf serum in cell culture, such as in culture of hybridoma cells for preparing monoclonal antibodies.
摘要翻译： PCT No.PCT / SE89 / 00232 Sec。 371日期1990年12月26日第 102（e）日期1990年12月26日PCT提交1989年4月26日PCT公布。第WO89 / 10398号PCT公开日期为1989年11月2日。血小板裂解液由来自动物全血的血浆产生，其含有柠檬酸盐，以防止储存期间血液凝结。裂解液通过离心血浆以产生富含血小板的糊剂制备，向浆液中加入钙以裂解其中的血小板并使纤维蛋白原凝结以产生含有裂解血小板的透明液体，无菌过滤液体并收集含有裂解血小板的液体滤液。获得的血小板溶解物可用于在细胞培养物中完全或部分替代胎牛血清，例如用于制备单克隆抗体的杂交瘤细胞的培养。

4. 发明授权

US5605816A Recombinant DNA encoding PDGF A-chain polypeptides 失效
标题翻译：编码PDGF A链多肽的重组DNA
公开(公告)号：US5605816A
公开(公告)日：1997-02-25
申请号：US454425
申请日：1995-05-30
申请人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
发明人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
IPC分类号： C07K14/49 , C12N9/02 , C12N15/18 , C12N15/62 , C12N15/81 , C12N15/85 , C12N15/19
CPC分类号： C12N9/0089 , C07K14/49 , C12N15/62 , C12N15/81 , C12N15/85 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N2800/108 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/36 , C12N2830/55 , C12N2830/702
摘要： DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
摘要翻译：编码两种形式的PDGF A链多肽的DNA，构建用于在酵母和哺乳动物细胞中表达这种DNA的表达载体，以及在酵母和哺乳动物细胞中表达这种DNA以产生活性PDGF A链同源二聚体和活性PDGFA- 链/ B链异源二聚体。

5. 发明授权

US5444151A Platelet derived growth factor antagonists 失效
标题翻译：血小板衍生生长因子拮抗剂
公开(公告)号：US5444151A
公开(公告)日：1995-08-22
申请号：US977234
申请日：1992-11-16
申请人： Flemming S. Vassbotn , Maria Andersson , Gudrun Backstrom , Ulla Engstrom , Carl-Henrik Heldin , Ulf Hellman , Arne stman , Bengt Westermark
发明人： Flemming S. Vassbotn , Maria Andersson , Gudrun Backstrom , Ulla Engstrom , Carl-Henrik Heldin , Ulf Hellman , Arne stman , Bengt Westermark
IPC分类号： C12N15/09 , A61K38/00 , A61K38/22 , C07H21/04 , C07K14/49 , C12N5/10 , C12N15/12 , C12P21/02 , C12R1/91 , C07K14/00 , C07K7/00 , C07K99/00
CPC分类号： C07K14/49 , A61K38/00 , Y10S930/12
摘要： The invention describes antagonists for PDGF. The antagonists contain amino acids, and may be monomers or dimers. Especially preferred are dimers which bind the PDGF receptors, but prevent dimerization of the bound receptors. Dimerization is necessary for PDGF effect, hence the antagonistic effect. Also described are nucleic acid sequences for making the antagonists, as well as cell lines transfected with the material.
摘要翻译：本发明描述了PDGF的拮抗剂。拮抗剂含有氨基酸，可以是单体或二聚体。特别优选的是结合PDGF受体但抑制结合受体的二聚化的二聚体。二聚化对于PDGF效应是必需的，因此是拮抗作用。还描述了用于制备拮抗剂的核酸序列，以及用该材料转染的细胞系。

6. 发明授权

US5219759A Recombinant DNA encoding PDGF A-chain polypeptide and expression vectors 失效
标题翻译：编码PDGF A链多肽和表达载体的重组DNA
公开(公告)号：US5219759A
公开(公告)日：1993-06-15
申请号：US574540
申请日：1990-08-27
申请人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
发明人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
IPC分类号： C07K14/49 , C12N9/02 , C12N15/18 , C12N15/62 , C12N15/81 , C12N15/85
CPC分类号： C12N9/0089 , C07K14/49 , C12N15/62 , C12N15/81 , C12N15/85 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N2800/108 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/36 , C12N2830/55 , C12N2830/702
摘要： DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
摘要翻译：编码两种形式的PDGF A链多肽的DNA，构建用于在酵母和哺乳动物细胞中表达这种DNA的表达载体，以及在酵母和哺乳动物细胞中表达这种DNA以产生活性PDGF A链同源二聚体和活性PDGFA- 链/ B链异源二聚体。

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