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1. 发明申请

US20190002892A1 METHODS AND COMPOSITIONS FOR PRODUCTION OF AROMATIC AND OTHER COMPOUNDS IN YEAST 审中-公开
公开(公告)号：US20190002892A1
公开(公告)日：2019-01-03
申请号：US16019822
申请日：2018-06-27
申请人： Iowa State University Research Foundation, Inc.
发明人： Zengyi Shao , Mingfeng Cao , Miguel Suastegui , Meirong Gao
IPC分类号： C12N15/81 , C12N9/10 , C12N9/02 , C12N9/88 , C12P5/00
CPC分类号： C12N15/81 , C12N9/0004 , C12N9/10 , C12N9/88 , C12N15/102 , C12N2830/002 , C12N2830/008 , C12N2830/702 , C12P5/007 , C12P7/42 , C12P7/44
摘要： The present disclosure includes methods and components for production of valuable industrial compounds in yeast. In an embodiment, the present invention provides a nucleic acid construct with increased stability for gene expression or gene editing comprising: a nucleic acid sequence encoding one or more of SEQ ID NO: 1-8 (CENs 1-8); and one or more regulatory elements functional in a yeast cell. In an embodiment of the present invention the nucleic acid constructs are vectors, preferably episomal vectors. High expression promoters, as well as methods for increasing production of compounds such as aromatics are disclosed.

2. 发明申请

US20120301922A1 NOVEL P. PASTORIS PASTORIS PROMOTERS, AND THE USE THEREOF TO DIRECT EXPRESSION OF PROTEINS IN YEAST, PREFERABLY USING A HAPLOID MATING STRATEGY 有权
标题翻译：新颖的PASTORIS PASTORIS促销者，以及它们用来直接表达蛋白质在YEAST中，优先使用HAPLOID MATING策略
公开(公告)号：US20120301922A1
公开(公告)日：2012-11-29
申请号：US13542851
申请日：2012-07-06
申请人： James M. Cregg , Ilya I. Tolstorukov
发明人： James M. Cregg , Ilya I. Tolstorukov
IPC分类号： C12N15/113 , C12P21/00 , C12N1/19 , C12N15/12 , C12N15/81
CPC分类号： C12N9/88 , C07K14/39 , C07K16/2809 , C12N9/0006 , C12N9/1205 , C12N15/81 , C12N15/815 , C12N2830/005 , C12N2830/702 , C12P21/02 , C12Y402/01011 , Y02P20/52
摘要： Novel promoters which are derived from P. pastoris pastoris which are inducible or repressible under specific growth conditions are provided. These promoters are useful for regulating the expression of a desired structural gene, e.g., a mammalian polypeptide. Particularly preferred is the use of these novel promoters to regulate gene expression in polyploidal yeast such as diploidal P. pastoris produced by mating or spheroplast fusion.
摘要翻译：提供了在特定生长条件下可诱导或阻抑的来自巴斯德一种巴斯德毕赤酵母的新型启动子。这些启动子可用于调节所需结构基因例如哺乳动物多肽的表达。特别优选使用这些新型启动子来调节多倍体酵母中的基因表达，例如通过交配或原生质球融合产生的二倍体巴斯德毕赤酵母。

3. 发明授权

US07527925B1 Purified pol DNA, a recombinant DNA construct for expression of HIV pol polypeptide sequences and a cell containing such construct 失效
标题翻译：纯化的pol DNA，用于表达HIV pol多肽序列的重组DNA构建体和含有此构建体的细胞
公开(公告)号：US07527925B1
公开(公告)日：2009-05-05
申请号：US08443433
申请日：1995-05-17
申请人： Paul A. Luciw , Dino Dina , Kathelyn Steimer , Ray Sanchez Pescador , Carlos George-Nascimento , Deborah Parkes , Rob Hallewell , Philip J. Barr , Martha Truett
发明人： Paul A. Luciw , Dino Dina , Kathelyn Steimer , Ray Sanchez Pescador , Carlos George-Nascimento , Deborah Parkes , Rob Hallewell , Philip J. Barr , Martha Truett
IPC分类号： A61K39/00 , C07H21/02
CPC分类号： C07K14/005 , A61K38/00 , C07K2319/00 , C12N9/0089 , C12N15/81 , C12N15/85 , C12N2740/16222 , C12N2830/00 , C12N2830/15 , C12N2830/55 , C12N2830/702 , C12N2830/704 , C12Q1/703 , Y10S435/948 , Y10S435/974
摘要： Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.
摘要翻译：提供多核苷酸序列用于诊断与淋巴结病综合征和/或获得性免疫缺陷综合征相关的人宿主中的逆转录病毒感染的存在，用于表达多肽和使用多肽来制备抗体，其中多肽和抗体可以是用作诊断试剂或用于治疗，例如疫苗和被动免疫。这些序列提供与所指示的综合征相关的病毒感染因子的检测，并可用于表达抗原多肽。

4. 发明授权

US06939849B1 Recombinant PDGF A-chain homodimers and methods of use 失效
标题翻译：重组PDGF A链同源二聚体及其使用方法
公开(公告)号：US06939849B1
公开(公告)日：2005-09-06
申请号：US08453350
申请日：1995-05-30
申请人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
发明人： Carl-Henrik Heldin , Christer Betsholtz , Bengt Westermark , Timothy J. Knott , James Scott , Graeme I. Bell
IPC分类号： C07K14/49 , C12N9/02 , C12N15/62 , C12N15/81 , C12N15/85 , A61K38/18 , C12N1/21 , C12N15/18 , C12N15/63
CPC分类号： C12N9/0089 , C07K14/49 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/75 , C12N15/62 , C12N15/81 , C12N15/85 , C12N2800/108 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/36 , C12N2830/55 , C12N2830/702
摘要： DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
摘要翻译：编码两种形式的PDGF A链多肽的DNA，构建用于在酵母和哺乳动物细胞中表达这种DNA的表达载体，以及在酵母和哺乳动物细胞中表达这种DNA以产生活性PDGF A链同源二聚体和活性PDGFA- 链/ B链异源二聚体。

5. 发明申请

US20040018625A1 Inducible methods for repressing gene function 审中-公开
标题翻译：抑制基因功能的诱导方法
公开(公告)号：US20040018625A1
公开(公告)日：2004-01-29
申请号：US10350799
申请日：2003-01-24
发明人： Kevin Struhl , Zarmik Moqtaderi
IPC分类号： C12N015/85 , A61K031/65
CPC分类号： C07K14/00 , C07K2319/00 , C12N15/635 , C12N15/67 , C12N15/85 , C12N2830/002 , C12N2830/005 , C12N2830/702
摘要： Methods for the rapid repression of gene function in eucaryotic cells are disclosed including inducible means for both shutting down a targeted gene's transcription and rapidly removing a targeted gene's polypeptide product.
摘要翻译：公开了在真核细胞中快速抑制基因功能的方法，包括用于关闭靶基因的转录并快速去除靶基因的多肽产物的诱导方法。

6. 发明授权

US6013432A Immunoassay of HIV antibodies using recombinant or synthetic selected pol sequence 失效
标题翻译：使用重组或合成选择的pol序列免疫测定HIV抗体
公开(公告)号：US6013432A
公开(公告)日：2000-01-11
申请号：US443434
申请日：1995-05-17
申请人： Paul A. Luciw , Dino Dina
发明人： Paul A. Luciw , Dino Dina
IPC分类号： A61K38/00 , C07K14/16 , C12N9/02 , C12N15/48 , C12N15/81 , C12N15/85 , C12Q1/70 , G01N33/53 , G01N33/569
CPC分类号： C07K14/005 , C12N15/81 , C12N15/85 , C12N9/0089 , C12Q1/703 , A61K38/00 , C07K2319/00 , C12N2740/16222 , C12N2830/00 , C12N2830/15 , C12N2830/55 , C12N2830/702 , C12N2830/704 , Y10S435/96 , Y10S435/974 , Y10S530/806 , Y10S530/81 , Y10S530/826
摘要： Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.
摘要翻译：提供多核苷酸序列用于诊断与淋巴结病综合征和/或获得性免疫缺陷综合征相关的人宿主中的逆转录病毒感染的存在，用于表达多肽和使用多肽来制备抗体，其中多肽和抗体可以是用作诊断试剂或用于治疗，例如疫苗和被动免疫。这些序列提供与所指示的综合征相关的病毒感染因子的检测，并可用于表达抗原多肽。

7. 发明授权

US5891429A Recombinant human granulocyte-macrophage-colony stimulating factor (GM-CSF) 失效
标题翻译：重组人粒细胞巨噬细胞集落刺激因子（GM-CSF）
公开(公告)号：US5891429A
公开(公告)日：1999-04-06
申请号：US466308
申请日：1995-06-06
申请人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong , Elizabeth A. Wang
发明人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong , Elizabeth A. Wang
IPC分类号： A61K38/00 , C07K14/535 , C12N15/27 , C12N15/70 , C12N15/81 , C12N15/85 , A61K38/19 , C07K1/20
CPC分类号： C12N15/85 , C07K14/535 , C12N15/70 , C12N15/81 , A61K38/00 , C12N2800/206 , C12N2830/00 , C12N2830/702 , C12N2840/105 , C12N2840/44
摘要： A method for preparing and isolating a transformation vector containing CSF/cDNA is described. The method comprises:preparing RNA from a cell that produces CSF;preparing polyadenylated messenger RNA from said RNA;preparing single stranded cDNA from said messenger RNA;converting the single stranded cDNA to double stranded cDNA;inserting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies;picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool;transfecting the plasmid DNA into suitable host cells for expressing CSF protein;culturing the transfected cells and assaying the supernatant for CSF activity; andselecting CSF positive pools and screening the colonies used to make the pool to identify a colony having CSF activity. Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line. The invention also provides a method of purifying the CSF proteins and the purified proteins so produced.
摘要翻译：描述了制备和分离含有CSF / cDNA的转化载体的方法。该方法包括：从产生CSF的细胞制备RNA; 从所述RNA制备聚腺苷酸化的信使RNA; 从所述信使RNA制备单链cDNA; 将单链cDNA转化为双链cDNA; 将双链cDNA插入转化载体并用所述载体转化细菌以形成菌落; 分别取200至500个菌落的池，每个池中分离出质粒DNA; 将质粒DNA转染到合适的宿主细胞中以表达CSF蛋白; 培养转染的细胞并测定上清液的CSF活性; 并选择CSF阳性池并筛选用于制备池的菌落以鉴定具有CSF活性的菌落。还描述了编码具有CSF活性的蛋白质（即CSF / cDNA）的cDNA，用含有这种CSF / cDNA的重组载体转化的微生物或细胞系，以及通过培养表达所述CSF / cDNA来产生CSF蛋白的方法微生物或细胞系。本发明还提供了纯化CSF蛋白质和如此制备的纯化蛋白质的方法。

8. 发明授权

US5705149A Use of interleukin-7 to stimulate proliferation of hematopoietic cell precursors 失效
公开(公告)号：US5705149A
公开(公告)日：1998-01-06
申请号：US446908
申请日：1995-05-22
申请人： Anthony E. Namen , Raymond G. Goodwin , Stephen D. Lupton , Diane Y. Mochizuki
发明人： Anthony E. Namen , Raymond G. Goodwin , Stephen D. Lupton , Diane Y. Mochizuki
IPC分类号： A61K38/00 , C07K14/54 , C07K16/24 , C12N15/62 , C12N15/70 , C12N15/81 , C12N15/85 , A61K38/20
CPC分类号： C07K16/244 , C07K14/5418 , C12N15/625 , C12N15/70 , C12N15/81 , C12N15/85 , A61K38/00 , C07K2317/34 , C07K2319/02 , C07K2319/036 , C07K2319/43 , C07K2319/50 , C07K2319/75 , C12N2800/108 , C12N2830/00 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/60 , C12N2830/702 , C12N2830/704 , C12N2830/85 , C12N2840/44 , Y10S514/885
摘要： Mammalian Interleukin-7 proteins (IL-7s), DNAs and expression vectors encoding mammalian IL-7s, and processes for producing mammalian IL-7s as products of cell culture, including recombinant systems, are disclosed.

9. 发明授权

US5688688A Vector for expression of a polypeptide in a mammalian cell 失效
标题翻译：用于在哺乳动物细胞中表达多肽的载体
公开(公告)号：US5688688A
公开(公告)日：1997-11-18
申请号：US288336
申请日：1994-08-10
申请人： Paul A. Luciw , Dino Dina , Steven Rosenberg , Barbara S. Chapman , Richard M. Thayer , Nancy L. Haigwood
发明人： Paul A. Luciw , Dino Dina , Steven Rosenberg , Barbara S. Chapman , Richard M. Thayer , Nancy L. Haigwood
IPC分类号： C12N15/09 , A61K31/00 , A61K38/00 , A61K39/21 , A61K39/395 , A61P31/00 , A61P31/12 , A61P31/14 , A61P31/18 , A61P37/00 , A61P37/02 , C07H21/04 , C07K14/005 , C07K14/155 , C07K14/16 , C07K14/195 , C07K16/00 , C07K19/00 , C12N1/19 , C12N1/21 , C12N7/00 , C12N7/02 , C12N9/02 , C12N15/00 , C12N15/48 , C12N15/49 , C12N15/81 , C12N15/85 , C12P21/02 , C12Q1/68 , C12Q1/70 , C12R1/19 , C12R1/865 , G01N33/53 , G01N33/569 , G01N33/577 , C12N15/67 , C12N15/11
CPC分类号： C07K14/005 , C12N15/81 , C12N15/85 , C12N9/0089 , C12Q1/703 , A61K38/00 , C07K2319/00 , C07K2319/40 , C07K2319/735 , C07K2319/75 , C12N2740/16122 , C12N2740/16222 , C12N2800/108 , C12N2830/00 , C12N2830/002 , C12N2830/005 , C12N2830/15 , C12N2830/36 , C12N2830/55 , C12N2830/702 , C12N2830/704 , Y10S435/81 , Y10S435/82 , Y10S435/974 , Y10S435/975 , Y10S530/826
摘要： Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.
摘要翻译：提供多核苷酸序列用于诊断与淋巴结病综合征和/或获得性免疫缺陷综合征相关的人宿主中的逆转录病毒感染的存在，用于表达多肽和使用多肽来制备抗体，其中多肽和抗体可以是用作诊断试剂或用于治疗，例如疫苗和被动免疫。这些序列提供与所指示的综合征相关的病毒感染因子的检测，并可用于表达抗原多肽。

10. 发明授权

US5629160A Diagnostic assay for inhibitor of tissue-type and urokinase-type plasminogen activators, and gene coding for the inhibitor 失效
标题翻译：组织型和尿激酶型纤溶酶原激活物抑制剂的诊断测定，以及抑制剂的基因编码
公开(公告)号：US5629160A
公开(公告)日：1997-05-13
申请号：US248348
申请日：1994-05-24
申请人： David J. Loskutoff , Tor Ny , Michael Sawdey
发明人： David J. Loskutoff , Tor Ny , Michael Sawdey
IPC分类号： C07K14/035 , C07K14/81 , C07K16/18 , C12N15/81 , C12N15/85 , G01N33/86 , G01N33/53
CPC分类号： G01N33/86 , C07K14/005 , C07K14/8132 , C07K16/18 , C12N15/81 , C12N15/85 , C07K2319/00 , C07K2319/02 , C07K2319/33 , C07K2319/61 , C12N2710/16622 , C12N2830/00 , C12N2830/702 , G01N2333/8132 , G01N2333/9723 , G01N2333/9726
摘要： A biological reagent and diagnostic system for the detection and quantitation of endothelial plasminogen activator inhibitor (PAI) are disclosed, as are substantially pure, recombinant human endothelial PAI, its biologically pure gene and a vector for cloning the gene and expressing a gene product.
摘要翻译：公开了用于检测和定量内皮纤溶酶原激活物抑制剂（PAI）的生物试剂和诊断系统，基本上纯的重组人内皮PAI，其生物学纯基因和用于克隆基因并表达基因产物的载体。

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