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1. 发明授权

US08735107B2 Lyase enzymes, nucleic acids encoding them and methods for making and using them 有权
标题翻译：裂解酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US08735107B2
公开(公告)日：2014-05-27
申请号：US13287567
申请日：2011-11-02
申请人： David P. Weiner , Alexander Varvak , Toby Richardson , Mircea Podar , Ellen Burke , Shaun Healey
发明人： David P. Weiner , Alexander Varvak , Toby Richardson , Mircea Podar , Ellen Burke , Shaun Healey
IPC分类号： C12P13/24 , C12P13/22
CPC分类号： C12N9/88 , C12P7/42 , C12P13/222 , C12P13/225 , C12P13/24 , C12Y403/01005 , C12Y403/01023 , D06M16/003
摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X═NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R═H or alkyl.
摘要翻译：本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。本发明的多肽可用于多种药物，农业和工业环境。在一个或几个o，m和p位置R = H或烷基时，X = NO 2，Cl，Br，NH 2，OH，H。

2. 发明申请

US20110027346A1 Lyase Enzymes, Nucleic Acids Encoding Them and Methods for Making and Using Them 审中-公开
标题翻译：裂解酶，编码它们的核酸及其制备和使用方法
公开(公告)号：US20110027346A1
公开(公告)日：2011-02-03
申请号：US12303088
申请日：2007-05-29
申请人： David Weiner , Alexander Varvak , Toby Richardson , Mircea Podar , Ellen Burke , Shaun Healey
发明人： David Weiner , Alexander Varvak , Toby Richardson , Mircea Podar , Ellen Burke , Shaun Healey
IPC分类号： A61K9/127 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , C12N1/15 , C12N1/19 , A61P7/00 , C12N9/88 , C12N11/16 , C12N11/14 , C12N11/08 , C40B40/10 , C12P7/40 , A61K38/51 , A61K9/14 , A23C9/12
CPC分类号： C12N9/88
摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.
摘要翻译：本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。本发明的多肽可用于多种药物，农业和工业环境。

3. 发明授权

US09234216B2 Variant CBH I polypeptides 有权
标题翻译：变体CBH I多肽
公开(公告)号：US09234216B2
公开(公告)日：2016-01-12
申请号：US13824315
申请日：2011-10-06
申请人： Justin T. Stege , Alexander Varvak , John Poland , Chris S. Lyon , Shaun Healey , Peter Luginbuhl
发明人： Justin T. Stege , Alexander Varvak , John Poland , Chris S. Lyon , Shaun Healey , Peter Luginbuhl
IPC分类号： C12P7/14 , C12N9/42 , C12P19/02 , C12P19/14
CPC分类号： C12P7/14 , C12N9/2437 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.
摘要翻译：在替代实施方案中，本发明提供具有木质纤维素分解（木质纤维素）活性的多肽，例如木质素分解和纤维素分解活性，包括例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶（cbhl）（例如，外切纤维二糖水解酶例如具有可以逐步释放纤维二糖单元和-1,4葡萄糖 - 葡萄糖二糖的“外显子”活性，β-葡糖苷酶，木聚糖酶，甘露聚糖，木糖苷酶（例如，（β-谷氨酸）和/或阿拉伯呋喃糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法在一个实施方案中，本发明提供了本发明的多肽的热稳定性和耐热性形式，使用本发明的多肽和核酸在各种制药，农业和工业环境中;例如，作为生物质生物转化的酶，例如木质纤维素残基，i 在一方面，这些糖用作生产乙醇和燃料的化学原料，例如生物燃料，例如合成液体或气体燃料，包括乙醇，甲醇等。

4. 发明授权

US09096871B2 Variant CBH I polypeptides with reduced product inhibition 有权
标题翻译：具有减少的产物抑制的变体CBH I多肽
公开(公告)号：US09096871B2
公开(公告)日：2015-08-04
申请号：US13824317
申请日：2011-10-06
申请人： Justin T. Stege , Alexander Varvak , John Poland , Chris S. Lyon , Shaun Healey , Peter Luginbuhl
发明人： Justin T. Stege , Alexander Varvak , John Poland , Chris S. Lyon , Shaun Healey , Peter Luginbuhl
IPC分类号： C12N9/42 , C12P7/14 , C12P19/02 , C12P19/14
CPC分类号： C12P7/14 , C12N9/2437 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.
摘要翻译：本公开涉及具有降低的产物抑制的变体CBH I多肽，以及包含变体CBH I多肽的组合物，例如纤维素酶组合物。变体CBH I多肽和相关组合物可用于各种农业和工业应用。本公开还涉及编码变体CBH I多肽的核酸和重组表达变体CBH I多肽的宿主细胞。

5. 发明申请

US20120177722A1 Lyaseenzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them 有权
标题翻译：裂解酶，核酸编码及其制备和使用方法
公开(公告)号：US20120177722A1
公开(公告)日：2012-07-12
申请号：US13287567
申请日：2011-11-02
申请人： David P. Weiner , Alexander Varvak , Toby Richardson , Mircea Podar , Ellen Burke , Shaun Healey
发明人： David P. Weiner , Alexander Varvak , Toby Richardson , Mircea Podar , Ellen Burke , Shaun Healey
IPC分类号： A61K9/127 , C12N15/63 , C12N9/88 , C40B50/06 , C40B30/00 , C12P7/40 , C12P7/42 , C12P7/22 , C12P13/22 , C12P17/10 , C12P13/24 , A61K38/51 , A61K31/7088 , A61K9/00 , A61K9/50 , A61K9/51 , A61K9/14 , A61P3/00 , C12N15/60 , B82Y5/00
CPC分类号： C12N9/88 , C12P7/42 , C12P13/222 , C12P13/225 , C12P13/24 , C12Y403/01005 , C12Y403/01023 , D06M16/003
摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and meth-°ds of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X═NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R═H or alkyl.
摘要翻译：本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。本发明的多肽可用于多种药物，农业和工业环境。在一个或几个o，m和p位置R = H或烷基时，X = NO 2，Cl，Br，NH 2，OH，H。

6. 发明授权

US08043839B2 Xylanases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：木聚糖酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：US08043839B2
公开(公告)日：2011-10-25
申请号：US12279326
申请日：2007-02-14
申请人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
发明人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
IPC分类号： C12N9/24 , C12N1/20 , C12N1/15 , C12N1/16 , C12N1/19 , C12N15/70 , C12P21/06 , C07H21/04 , A01H5/00
CPC分类号： C12N9/248 , A23K10/12 , A23K20/163 , A23L5/00 , A23L7/00 , A23L29/30 , A23L31/00 , A23L31/10 , A23L33/125 , A23V2002/00 , C12N9/2405 , C12N9/2437 , C12N9/2482 , C12N9/2488 , C12N9/2494 , C12P19/02 , C12P19/14 , C12Y302/01008 , D06L4/40 , D06L4/70 , D21C3/003 , D21C9/10 , D21H17/005 , Y02E50/16
摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及具有木聚糖酶，甘露聚糖酶和/或葡聚糖酶活性的酶，例如催化内部-1,4-木糖苷键或内切-1,4-羟基化酶键的水解; 和/或将线性多糖β-1,4-木聚糖降解成木糖。因此，本发明提供了分解作为植物细胞壁的主要成分的半纤维素的方法和方法，包括在任何植物或木材或木制品，木材废料，纸浆，纸制品中水解半纤维素的方法和方法，废纸或副产品。此外，还提供了设计新的木聚糖酶，甘露聚糖酶和/或葡聚糖酶的方法及其使用方法。木聚糖酶，甘露聚糖酶和/或葡聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

7. 发明申请

US20090155238A1 XYLANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权
标题翻译： XYLANASES，核酸编码它们及其制备和使用方法
公开(公告)号：US20090155238A1
公开(公告)日：2009-06-18
申请号：US12279326
申请日：2007-02-14
申请人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
发明人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
IPC分类号： A61K38/47 , C12N15/11 , C07H21/04 , C12N15/00 , C12N5/06 , C12N1/21 , C12N1/19 , C12P7/02 , A23K1/165 , D06L3/11 , C12P7/10 , D21C3/00 , C12N5/04 , A01K67/027 , A01H5/00 , C12N9/24 , C12P21/04
CPC分类号： C12N9/248 , A23K10/12 , A23K20/163 , A23L5/00 , A23L7/00 , A23L29/30 , A23L31/00 , A23L31/10 , A23L33/125 , A23V2002/00 , C12N9/2405 , C12N9/2437 , C12N9/2482 , C12N9/2488 , C12N9/2494 , C12P19/02 , C12P19/14 , C12Y302/01008 , D06L4/40 , D06L4/70 , D21C3/003 , D21C9/10 , D21H17/005 , Y02E50/16
摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及具有木聚糖酶，甘露聚糖酶和/或葡聚糖酶活性的酶，例如催化内部β-1,4-木糖苷键或内-A-1,4-ghicanase键的水解; 和/或将线性多糖β-1,4-木聚糖降解成木糖。因此，本发明提供了分解作为植物细胞壁的主要成分的半纤维素的方法和方法，包括在任何植物或木材或木制品，木材废料，纸浆，纸制品中水解半纤维素的方法和方法，废纸或副产品。此外，还提供了设计新的木聚糖酶，甘露聚糖酶和/或葡聚糖酶的方法及其使用方法。木聚糖酶，甘露聚糖酶和/或葡聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

8. 发明申请

US20080289056A1 CHEMOENZYMATIC METHODS FOR THE SYNTHESIS OF STATINS AND STATIN INTERMEDIATES 有权
标题翻译：统计学和中性体合成的化学方法
公开(公告)号：US20080289056A1
公开(公告)日：2008-11-20
申请号：US12032337
申请日：2008-02-15
申请人： William GREENBERG , Mark J. Burk , Alexander Varvak , Kelvin Wong
发明人： William GREENBERG , Mark J. Burk , Alexander Varvak , Kelvin Wong
IPC分类号： A01K67/027 , C12N15/11 , C12N15/00 , C12N9/00 , C12P21/04 , C07K1/00 , C12P17/06 , C12N15/82 , C12Q1/68 , C07K16/18 , A01H5/00 , C12N5/06
CPC分类号： C12P13/004 , C12N9/88 , C12P7/42 , C12P17/02 , C12P17/06 , C12P17/10 , C12P17/12
摘要： The invention provides aldolases, nucleic acids encoding them and methods for making and using them, including chemoenzymatic processes for making β,δ-dihydroxyheptanoic acid side chains and compositions comprising these side chains, e.g., [R—(R*,R*)]-2-(4-fluorophenyl)-b,d-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)-carbonyl]-1H-pyrrole-1-heptanoic acid (atorvastatin, LIPITOR™), rosuvastatin (CRESTOR™), fluvastatin (LESCOL™), related compounds and their intermediates.
摘要翻译：本发明提供醛缩酶，编码它们的核酸和制备和使用它们的方法，包括制备β，δ-二羟基庚酸侧链的化学酶法和包含这些侧链的组合物，例如[R-（R *，R *）] -2-（4-氟苯基）-b，d-二羟基-5-（1-甲基乙基）-3-苯基-4-（苯基氨基） - 羰基] -1H-吡咯-1-庚酸（阿托伐他汀，LIPITOR ）），罗苏伐他汀（CRESTOR TM），氟伐他汀（LESCOL TM）），相关化合物及其中间体。

9. 发明授权

US07414119B2 Aldolases, nucleic acids encoding them and methods for making and using them 失效
标题翻译：醛缩酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US07414119B2
公开(公告)日：2008-08-19
申请号：US10472157
申请日：2003-08-19
申请人： William Greenberg , Mark J. Burk , Alexander Varvak , Kelvin Wong
发明人： William Greenberg , Mark J. Burk , Alexander Varvak , Kelvin Wong
IPC分类号： C12N15/60 , C12N15/62 , C12N15/79 , C12N9/88 , C12N17/06
CPC分类号： C12P13/004 , C12N9/88 , C12P7/42 , C12P17/02 , C12P17/06 , C12P17/10 , C12P17/12
摘要： The invention provides novel aldolases, nucleic acids encoding them and methods for making and using them, including chemoenzymatic processes for making β,δ-dihydroxyheptanoic acid side chains and compositions comprising these side chains, e.g., [R-(R*,R*)]-2-(4-fluorophenyl)-b,d-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)-carbonyl]-1H-pyrrole-1-heptanoic acid (atorvastatin, LIPITOR™), rosuvastatin (CRESTOR™), fluvastatin (LESCOL™), related compounds and their intermediates.
摘要翻译：本发明提供了新的醛缩酶，编码它们的核酸以及制备和使用它们的方法，包括制备β，δ-二羟基庚酸侧链的化学酶法和包含这些侧链的组合物，例如[R-（R *，R *） ] -2-（4-氟苯基）-b，d-二羟基-5-（1-甲基乙基）-3-苯基-4-（苯基氨基） - 羰基] -1H-吡咯-1-庚酸（阿托伐他汀，LIPITOR TM）），罗苏伐他汀（CRESTOR TM），氟伐他汀（LESCOL TM）），相关化合物及其中间体。

10. 发明申请

US20140147894A1 VARIANT CBH I POLYPEPTIDES WITH REDUCED PRODUCT INHIBITION 有权
公开(公告)号：US20140147894A1
公开(公告)日：2014-05-29
申请号：US13824317
申请日：2011-10-06
申请人： Justin T. Stege , Alexander Varvak , John Poland , Chris S. Lyon , Shaun Healey , Peter Luginhuhl
发明人： Justin T. Stege , Alexander Varvak , John Poland , Chris S. Lyon , Shaun Healey , Peter Luginhuhl
IPC分类号： C12N9/42 , C12P7/14 , C12P19/02 , C12P19/14
CPC分类号： C12P7/14 , C12N9/2437 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.

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