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1. 发明授权

US5681726A Method of double stranded DNA synthesis 失效
标题翻译：双链DNA合成方法
公开(公告)号：US5681726A
公开(公告)日：1997-10-28
申请号：US116049
申请日：1993-09-02
申请人： William David Huse , Connie Jo Hansen
发明人： William David Huse , Connie Jo Hansen
IPC分类号： C12N15/10 , C12P19/34
CPC分类号： C12N15/1096
摘要： The present invention provides an improved method for the synthesis of double stranded DNA, particularly complementary DNA for the construction of directional complementary DNA libraries. The method comprises synthesizing a first strand of DNA complementary to a selected RNA or DNA template by contacting with the template a linker/primer comprising a selected restriction site, a suitable RNA or DNA dependent DNA polymerase, and substrates comprising a deoxyribonucleotide triphosphate analog. The linker/primer and deoxyribonucleotide triphosphate analog are selected such that incorporation of the nucleotide analog in the first strand substantially protects the double stranded DNA from cleavage, under conditions sufficient to cleave or substantially cleave the linker/primer, at the selected restriction site.
摘要翻译：本发明提供了用于合成双链DNA，特别是用于构建定向互补DNA文库的互补DNA的改进方法。该方法包括通过与模板接触来合成与所选择的RNA或DNA模板互补的第一链，包含选定的限制性位点，合适的RNA或DNA依赖性DNA聚合酶的接头/引物，以及包含脱氧核糖核苷酸三磷酸类似物的底物。选择接头/引物和脱氧核苷酸三磷酸类似物，使得在所选择的限制性位点处，在足以切割或基本上切割接头/引物的条件下，在第一链中的核苷酸类似物的引入基本上保护双链DNA不被切割。

2. 发明授权

US09085762B2 Compositions and methods using split polymerases 有权
标题翻译：使用裂解聚合酶的组合物和方法
公开(公告)号：US09085762B2
公开(公告)日：2015-07-21
申请号：US11968152
申请日：2007-12-31
申请人： Holly Hogrefe , Lydia Wu , Jeffrey D. Fox , Connie Jo Hansen
发明人： Holly Hogrefe , Lydia Wu , Jeffrey D. Fox , Connie Jo Hansen
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.
摘要翻译：本发明涉及使用由至少两个稳定缔合以形成单个聚合酶的离散多肽组成的裂解聚合酶的组合物和方法。本发明还涉及用于表达本发明的裂解聚合酶的核酸构建体，以及使用本发明的裂解聚合酶的方法。本发明的酶在许多应用中可用于要求可检测的核酸标记，并且在定量PCR（QPCR）和DNA测序应用中特别有用。

3. 发明授权

US07642079B2 Proteases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：蛋白酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US07642079B2
公开(公告)日：2010-01-05
申请号：US10530643
申请日：2003-10-10
申请人： Michelle Cayouette , Connie Jo Hansen , Amy McClure , May Sun , Svetlana Gramatikova , Mark Dycaico , Nelson R. Barton , Justin T. Stege , Nahla M. Aboushadi
发明人： Michelle Cayouette , Connie Jo Hansen , Amy McClure , May Sun , Svetlana Gramatikova , Mark Dycaico , Nelson R. Barton , Justin T. Stege , Nahla M. Aboushadi
IPC分类号： C12N9/48
CPC分类号： C12N9/52 , A01K2217/05
摘要： The invention is directed to polypeptides having protease activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of diagnostic, therapeutic, and industrial contexts. The polypeptides of the invention can be used as, e.g., an additive for a detergent, for processing foods and for chemical synthesis utilizing a reverse reaction. Additionally, the polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to ethanol diodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in increasing starch yield from corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.
摘要翻译：本发明涉及具有蛋白酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。本发明的多肽可用于多种诊断，治疗和工业背景。本发明的多肽可用作例如用于洗涤剂的添加剂，用于加工食品和利用反向反应进行化学合成。此外，本发明的多肽可用于食品加工，酿造，浴添加剂，醇生产，肽合成，对映选择性，皮革工业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗，丝绸脱胶，生物膜降解，生物质转化为乙醇二聚体，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，用于增加玉米湿法研磨的淀粉产量，以及药物如消化助剂和抗炎（抗炎）剂。

4. 发明申请

US20080227159A1 Compositions and Methods Using Split Polymerases 有权
标题翻译：使用分裂聚合酶的组合物和方法
公开(公告)号：US20080227159A1
公开(公告)日：2008-09-18
申请号：US11968152
申请日：2007-12-31
申请人： Holly Hogrefe , Lydia Wu , Jeffrey D. Fox , Connie Jo Hansen
发明人： Holly Hogrefe , Lydia Wu , Jeffrey D. Fox , Connie Jo Hansen
IPC分类号： C12P19/34 , C12N9/10 , C07H21/00
CPC分类号： C12N9/1252
摘要： The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.
摘要翻译：本发明涉及使用由至少两个稳定缔合以形成单个聚合酶的离散多肽组成的裂解聚合酶的组合物和方法。本发明还涉及用于表达本发明的裂解聚合酶的核酸构建体，以及使用本发明的裂解聚合酶的方法。本发明的酶在许多应用中可用于要求可检测的核酸标记，并且在定量PCR（QPCR）和DNA测序应用中特别有用。

5. 发明授权

US08268605B2 Compositions and methods utilizing DNA polymerases 有权
标题翻译：使用DNA聚合酶的组合物和方法
公开(公告)号：US08268605B2
公开(公告)日：2012-09-18
申请号：US09896923
申请日：2001-06-29
申请人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
发明人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
IPC分类号： C12N9/12
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
摘要翻译：本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

6. 发明授权

US08043839B2 Xylanases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：木聚糖酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：US08043839B2
公开(公告)日：2011-10-25
申请号：US12279326
申请日：2007-02-14
申请人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
发明人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
IPC分类号： C12N9/24 , C12N1/20 , C12N1/15 , C12N1/16 , C12N1/19 , C12N15/70 , C12P21/06 , C07H21/04 , A01H5/00
CPC分类号： C12N9/248 , A23K10/12 , A23K20/163 , A23L5/00 , A23L7/00 , A23L29/30 , A23L31/00 , A23L31/10 , A23L33/125 , A23V2002/00 , C12N9/2405 , C12N9/2437 , C12N9/2482 , C12N9/2488 , C12N9/2494 , C12P19/02 , C12P19/14 , C12Y302/01008 , D06L4/40 , D06L4/70 , D21C3/003 , D21C9/10 , D21H17/005 , Y02E50/16
摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及具有木聚糖酶，甘露聚糖酶和/或葡聚糖酶活性的酶，例如催化内部-1,4-木糖苷键或内切-1,4-羟基化酶键的水解; 和/或将线性多糖β-1,4-木聚糖降解成木糖。因此，本发明提供了分解作为植物细胞壁的主要成分的半纤维素的方法和方法，包括在任何植物或木材或木制品，木材废料，纸浆，纸制品中水解半纤维素的方法和方法，废纸或副产品。此外，还提供了设计新的木聚糖酶，甘露聚糖酶和/或葡聚糖酶的方法及其使用方法。木聚糖酶，甘露聚糖酶和/或葡聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

7. 发明申请

US20090155238A1 XYLANASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权
标题翻译： XYLANASES，核酸编码它们及其制备和使用方法
公开(公告)号：US20090155238A1
公开(公告)日：2009-06-18
申请号：US12279326
申请日：2007-02-14
申请人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
发明人： David Weiner , David Blum , Alexander Varvak , Shaun Healey , Kristine Chang , Geoff Hazlewood , Thomas Todaro , Grace Desantis , Hwai Chang , Connie Jo Hansen , Scott W. Beaver , Thomas Woodward , Charles Hancock
IPC分类号： A61K38/47 , C12N15/11 , C07H21/04 , C12N15/00 , C12N5/06 , C12N1/21 , C12N1/19 , C12P7/02 , A23K1/165 , D06L3/11 , C12P7/10 , D21C3/00 , C12N5/04 , A01K67/027 , A01H5/00 , C12N9/24 , C12P21/04
CPC分类号： C12N9/248 , A23K10/12 , A23K20/163 , A23L5/00 , A23L7/00 , A23L29/30 , A23L31/00 , A23L31/10 , A23L33/125 , A23V2002/00 , C12N9/2405 , C12N9/2437 , C12N9/2482 , C12N9/2488 , C12N9/2494 , C12P19/02 , C12P19/14 , C12Y302/01008 , D06L4/40 , D06L4/70 , D21C3/003 , D21C9/10 , D21H17/005 , Y02E50/16
摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及具有木聚糖酶，甘露聚糖酶和/或葡聚糖酶活性的酶，例如催化内部β-1,4-木糖苷键或内-A-1,4-ghicanase键的水解; 和/或将线性多糖β-1,4-木聚糖降解成木糖。因此，本发明提供了分解作为植物细胞壁的主要成分的半纤维素的方法和方法，包括在任何植物或木材或木制品，木材废料，纸浆，纸制品中水解半纤维素的方法和方法，废纸或副产品。此外，还提供了设计新的木聚糖酶，甘露聚糖酶和/或葡聚糖酶的方法及其使用方法。木聚糖酶，甘露聚糖酶和/或葡聚糖酶在增加的pH和温度下具有增加的活性和稳定性。

8. 发明申请

US20080280291A1 Compositions and methods utilizing DNA polymerases 有权
公开(公告)号：US20080280291A1
公开(公告)日：2008-11-13
申请号：US11435018
申请日：2006-05-15
申请人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
发明人： Joseph A. Sorge , Connie Jo Hansen , Holly Hogrefe
IPC分类号： C12Q1/68 , C12N9/00
CPC分类号： B82Y10/00 , B82Y5/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.

9. 发明申请

US20090221029A1 PFU REPLICATION ACCESSORY FACTORS AND METHODS OF USE 审中-公开
标题翻译： PFU复制附件因素及使用方法
公开(公告)号：US20090221029A1
公开(公告)日：2009-09-03
申请号：US12258288
申请日：2008-10-24
申请人： Holly Hurlbut Hogrefe , Janice Marie Cline , Connie Jo Hansen
发明人： Holly Hurlbut Hogrefe , Janice Marie Cline , Connie Jo Hansen
IPC分类号： C12P21/06 , C07H21/04 , C12N15/63 , C12N1/21
CPC分类号： C12Q1/6844 , C07K14/195 , C12N9/00 , C12N9/1252 , C12N9/22 , C12Q1/686 , C12Q2521/101 , C12Q2527/125
摘要： This invention provides isolated polynucleotides that encode replication accessory factors. The invention also provides novel DNA replication accessory factors, which have been isolated and purified from the hyperthermophilic archaea Pyrococcus furiosus. The invention also provides various methods of enhancing a nucleic acid polymerase reaction comprising the addition of the replication accessory factors to the reaction. This invention further provides methods of synthesizing, amplifying, and mutagenizing nucleic acids of interest employing the replication accessory factors. This invention also provides kits comprising, at least one of the replication accessory factors. This invention also provides kits useful for various methods that comprise at least one replication accessory factor.
摘要翻译：本发明提供编码复制辅助因子的分离的多核苷酸。本发明还提供了新的DNA复制附属因子，其已经从超嗜热古细菌火球菌（Pyrococcus furiosus）中分离和纯化。本发明还提供增强核酸聚合酶反应的各种方法，包括向反应中加入复制辅助因子。本发明进一步提供了使用复制辅助因子合成，扩增和诱变感兴趣的核酸的方法。本发明还提供了包含至少一个复制附属因子的试剂盒。本发明还提供了可用于包含至少一个复制辅助因子的各种方法的试剂盒。

10. 发明授权

US6143531A Method of double stranded DNA synthesis 失效
标题翻译：双链DNA合成方法
公开(公告)号：US6143531A
公开(公告)日：2000-11-07
申请号：US899029
申请日：1997-07-22
申请人： William David Huse , Connie Jo Hansen
发明人： William David Huse , Connie Jo Hansen
IPC分类号： C12N15/10 , C12P19/34 , C07H21/04
CPC分类号： C12N15/1096
摘要： The present invention provides an improved method for the synthesis of double stranded DNA, particularly complementary DNA for the construction of directional complementary DNA libraries. The method comprises synthesizing a first strand of DNA complementary to a selected RNA or DNA template by contacting with the template a linker/primer comprising a selected restriction site, a suitable RNA or DNA dependent DNA polymerase, and substrates comprising a deoxyribonucleotide triphosphate analog. The linker/primer and deoxyribonucleotide triphosphate analog are selected such that incorporation of the nucleotide analog in the first strand substantially protects the double stranded DNA from cleavage, under conditions sufficient to cleave or substantially cleave the linker/primer, at the selected restriction site.
摘要翻译：本发明提供了用于合成双链DNA，特别是用于构建定向互补DNA文库的互补DNA的改进方法。该方法包括通过与模板接触来合成与所选择的RNA或DNA模板互补的第一链，包含选定的限制性位点，合适的RNA或DNA依赖性DNA聚合酶的接头/引物，以及包含脱氧核糖核苷酸三磷酸类似物的底物。选择接头/引物和脱氧核苷酸三磷酸类似物，使得在所选择的限制性位点处，在足以切割或基本上切割接头/引物的条件下，在第一链中的核苷酸类似物的引入基本上保护双链DNA不被切割。

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