专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07001725B2 Kits employing generalized target-binding e-tag probes 失效
标题翻译：套件采用广义目标绑定电子标签探针
公开(公告)号：US07001725B2
公开(公告)日：2006-02-21
申请号：US09824851
申请日：2001-04-02
申请人： Sharat Singh , Tracy Matray , Ahmed Chenna
发明人： Sharat Singh , Tracy Matray , Ahmed Chenna
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , B65D85/42 , G01N27/26
CPC分类号： C07H19/10 , C07H19/06 , C07H21/00 , Y10S435/808
摘要： Kits for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, a capture agent and optionally a cleaving agent. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了用于多个检测一种或多种配体和靶抗配糖体的结合或相互作用的试剂盒。检测涉及到识别标签作为目标识别的结果。试剂盒包括电泳标签探针或电子标签探针，捕获剂和任选的裂解剂。电子标签探针包括检测区域和称为迁移率调节剂的迁移率限定区域，两者都与靶结合部分连接。在使用试剂盒时，将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

2. 发明授权

US06908594B1 Efficient microfluidic sealing 有权
标题翻译：高效的微流体密封
公开(公告)号：US06908594B1
公开(公告)日：2005-06-21
申请号：US09691957
申请日：2000-10-18
申请人： Samuel Benjamin Schaevitz , Travis Boone , Torleif Ove Bjornson
发明人： Samuel Benjamin Schaevitz , Travis Boone , Torleif Ove Bjornson
IPC分类号： B01L3/00 , B01L3/02 , B01L9/00 , B01L99/00 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/06 , B01L11/00
CPC分类号： B01L3/5025 , B01J2219/00315 , B01J2219/00317 , B01J2219/00331 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01J2219/00804 , B01J2219/0086 , B01J2219/00869 , B01L3/502715 , B01L3/50853 , B01L2200/0689 , B01L2300/041 , B01L2300/0829 , B01L2400/0415 , C40B40/06 , C40B40/10 , C40B60/14 , Y10T436/11 , Y10T436/117497 , Y10T436/118339
摘要： Improved sealing for microstructures in microfluidic devices having a plurality of units is provided by providing collars surrounding the openings to the microstructures, such as reservoirs. The collars are protrusions extending from the surface of the devices and the internal walls of the collars generally aligned with the internal walls of the microstructure. Conformable and/or adhesive lids are employed for sealing the microstructures.
摘要翻译：通过向微观结构（例如储存器）提供围绕开口的套环来提供具有多个单元的微流体装置中用于微结构的改进的密封。套环是从装置的表面延伸的凸起，并且套环的内壁与微观结构的内壁大致对准。采用适形和/或粘合剂盖来密封微结构。

3. 发明授权

US06846645B2 Multiplexed enzymatic assays 失效
标题翻译：多重酶检测
公开(公告)号：US06846645B2
公开(公告)日：2005-01-25
申请号：US10613583
申请日：2003-07-02
申请人： Qifeng Xue , Ian Gibbons
发明人： Qifeng Xue , Ian Gibbons
IPC分类号： G01N33/50 , C12Q1/00 , C12Q1/48 , G01N33/15 , G01N33/561 , B01D57/02 , C12Q1/02 , G01N33/53
CPC分类号： G01N33/561 , C12Q1/00 , C12Q1/485 , G01N2333/9121 , G01N2333/91215 , G01N2500/04 , G01N2500/20 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A mutiplexed enzyme assay method and substrate set for performing the method are disclosed. The method includes performing a plurality of enzyme reactions in the presence of a plurality of enzymes substrates, under conditions effective to convert an enzyme substrate to a corresponding product, where the product of each substrate and the substrate have different separation characteristics from each other and from the other substrates and their corresponding products. After performing the reactions, which may be carried out in separate or combined reactions, the substrates and products in said reactions are separated in a single separation medium. For each separated product and substrate, a separation characteristic effective to identify that product and substrate and a signal related to the amount of the product and substrate is detected. From this, one can determine the amount of substrate converted to the corresponding product in each of the reactions.
摘要翻译：公开了用于执行该方法的复合酶测定方法和底物组。该方法包括在有效将酶底物转化成相应产物的条件下，在多个酶底物的存在下进行多个酶反应，其中每个底物和底物的产物彼此具有不同的分离特性其他基材及其相应的产品。在进行可以在单独或组合反应中进行的反应之后，所述反应中的底物和产物在单一分离介质中分离。对于每个分离的产物和底物，检测到有效地鉴定该产物和底物的分离特性和与产物和底物的量相关的信号。由此，可以确定在每个反应中转化成相应产物的底物的量。

4. 发明申请

US20030017467A1 Multiple-site sample-handling apparatus and method 审中-公开
标题翻译：多站点样品处理设备和方法
公开(公告)号：US20030017467A1
公开(公告)日：2003-01-23
申请号：US09938439
申请日：2001-08-23
申请人： Aclara BioSciences, Inc.
发明人： Herbert H. Hooper , Sharat Singh , Travis D. Boone , Rolfe Anderson , David Albagli , Antonio J. Ricco
IPC分类号： G01N033/00
CPC分类号： B01J19/0046 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00454 , B01J2219/00495 , B01J2219/00511 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01J2219/00783 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/0086 , B01J2219/00873 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502784 , B01L3/527 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2200/16 , B01L2300/0636 , B01L2300/087 , B01L2300/0883 , B01L2300/0887 , B01L2400/0409 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B82Y30/00 , C40B40/06 , C40B40/10 , C40B50/14 , C40B60/14 , Y10T436/2575
摘要： A microchannel apparatus and method for processing a sample are disclosed. The apparatus include a multisite reaction channel, one or more sample-preparation stations upstream of the reaction channel, for carrying out one or more selected sample-preparation steps effective to convert a sample to the bulk-phase medium, and one or more product-processing stations downstream of the reaction channel, for processing products generated in one or more of the reaction regions. Also included is structure for transferring solvent or solvent components between one of the sample-preparation stations and one or more selected reaction regions in the reaction channel, and between one or more selected reaction regions in the reaction channel and one of said product-processing stations, under the control of a control unit.
摘要翻译：公开了一种用于处理样品的微通道装置和方法。该装置包括多反应通道，在反应通道上游的一个或多个样品制备站，用于进行有效将样品转化成体相介质的一个或多个选定的样品制备步骤，以及一个或多个产物 - 在反应通道下游的处理站，用于处理在一个或多个反应区域中产生的产物。还包括用于在一个样品制备工位之间和反应通道中的一个或多个选择的反应区域之间以及在反应通道中的一个或多个选择的反应区域和所述产物加工站之一之间转移溶剂或溶剂组分的结构在控制单元的控制下。

5. 发明申请

US20020189946A1 Microfluidic injection and separation system and method 审中-公开
标题翻译：微流控注射分离系统及方法
公开(公告)号：US20020189946A1
公开(公告)日：2002-12-19
申请号：US10113170
申请日：2002-03-29
申请人： Aclara BioSciences, Inc.
发明人： Ann K. Wainright , Stephen J. Williams
IPC分类号： G01N027/26 , G01N027/447
CPC分类号： B01L3/502784 , B01L2200/0605 , B01L2400/0421 , C07K1/28 , G01N27/44743 , G01N27/44747 , G01N27/44773 , G01N27/44791
摘要： Methods of sample loading and separation in a microfluidics device are described. The methods provide high resolution and high signal intensity, using, in a preferred embodiment, a simple two-electrode injection scheme with isotachophoretic (ITP) stacking, followed by ZE separation in the same channel.
摘要翻译：描述微流体装置中样品加载和分离的方法。所述方法提供高分辨率和高信号强度，在优选实施方案中，使用具有异羟基磷酸（ITP）层叠的简单双电极注入方案，然后在相同通道中进行ZE分离。

6. 发明申请

US20020142329A1 Compositions and methods employing cleavable electrophoretic tag reagents 审中-公开
标题翻译：使用可切割电泳标签试剂的组合物和方法
公开(公告)号：US20020142329A1
公开(公告)日：2002-10-03
申请号：US10008573
申请日：2001-11-09
申请人： Aclara BioSciences, Inc.
发明人： Tracy Matray , Vincent Hernandez , Sharat Singh
IPC分类号： C12Q001/68 , G01N033/53 , C07H021/04 , C07K016/46
CPC分类号： C07H21/04 , C07H1/06 , C07H21/00 , C12Q1/68 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00 , G01N33/561
摘要： Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The methods employ compositions comprising luminescent molecules such as, for example, fluorescent molecules, which are modified to provide for electrophoretic properties that differ for each modified luminescent molecule while maintaining substantially the same absorption, emission and quantum yield properties of the original luminescent molecule. The compositions may be cleavably linked to binding molecules to form the e-tag probes.
摘要翻译：提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。检测涉及到识别标签作为目标识别的结果。探针组包括电泳标签探针或e-标签探针，其包含检测区域和称为迁移率调节剂的迁移率限定区域，两者都连接到靶结合部分。将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。该方法使用包含发光分子（例如荧光分子）的组合物，其被修饰以提供对于每个修饰的发光分子不同的电泳性质，同时保持原始发光分子的基本相同的吸收，发射和量子产率性质。组合物可以与结合分子可裂解连接以形成e-标签探针。

7. 发明申请

US20020053399A1 Methods for fabricating enclosed microchannel structures 审中-公开
标题翻译：封闭微通道结构的制造方法
公开(公告)号：US20020053399A1
公开(公告)日：2002-05-09
申请号：US10016595
申请日：2001-12-07
申请人： Aclara Biosciences, Inc
发明人： David S. Soane , Herbert H. Hooper , M. Goretty Alonso-Amigo
IPC分类号： G09G003/32 , B32B031/00 , G01L001/20 , C07K001/26 , C02F001/469 , G01F001/64 , C08F002/58 , C25B007/00 , G01L009/18 , C25B015/00 , G01N027/26 , B01D057/02 , B01D059/42 , B01D059/50 , B01D061/42 , B01D061/58 , G01R001/00 , C02F001/40 , C25B009/00 , C02F011/00 , C25B011/00 , C25B013/00 , G01N027/27 , G01N027/403 , G01N027/453
CPC分类号： B29C65/02 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0887 , B29C65/1403 , B29C65/1406 , B29C65/1412 , B29C65/1425 , B29C65/48 , B29C65/4815 , B29C65/483 , B29C65/4835 , B29C65/484 , B29C65/4845 , B29C65/485 , B29C65/4865 , B29C65/524 , B29C66/5346 , B29C66/54 , B29C66/71 , B29C66/712 , B29L2031/756 , B29L2031/757 , G01N27/44704 , G01N27/44743 , G01N27/44752 , G01N27/44791 , B29K2023/06 , B29K2033/12 , B29K2067/003 , B29K2083/00
摘要： Methods are provided for the fabrication of polymeric microchannel structures having enclosed microchannels of capillary dimension. The microchannel structures are constructed of a base plate and a cover, sealed together. Microchannel structures having walls of a plastic material are formed in a generally planar surface of at least the base plate. The cover has at least one generally planar surface, and the microchannel structures are enclosed by bonding the planar surfaces of the cover and the base plate together. In some embodiments the surfaces of the cover and base plate are both of plastic material, and are directly thermally bonded. In some embodiments a bonding material is applied to one of the surface prior to bringing the surfaces together. Suitable bonding materials are disclosed.
摘要翻译：提供了用于制造具有毛细管尺寸的封闭微通道的聚合物微通道结构的方法。微通道结构由基板和盖构成，密封在一起。具有塑料材料壁的微通道结构形成在至少基板的大致平坦的表面上。盖具有至少一个大致平坦的表面，并且通过将盖和基板的平面表面粘合在一起来封闭微通道结构。在一些实施例中，盖和基板的表面都是塑料材料，并且是直接热粘结的。在一些实施例中，在使表面在一起之前，将粘合材料施加到该表面之一。公开了合适的粘合材料。

8. 发明授权

US6074827A Microfluidic method for nucleic acid purification and processing 失效
标题翻译：用于核酸纯化和加工的微流控法
公开(公告)号：US6074827A
公开(公告)日：2000-06-13
申请号：US18918
申请日：1998-02-05
申请人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
发明人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
IPC分类号： G01N33/48 , B01L3/00 , B01L7/00 , C12M1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/53 , G01N33/569 , C07H21/00 , C12P19/34
CPC分类号： G01N27/44743 , B01L3/50273 , B01L3/502753 , B01L3/502761 , G01N27/44791 , G01N33/5002 , G01N33/569 , B01F13/0059 , B01L2200/0631 , B01L2200/0668 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0877 , B01L2400/0409 , B01L2400/0415 , B01L2400/0421 , B01L7/52
摘要： Integrated microfluidic devices comprising at least an enrichment channel and a main electrophoretic flowpath are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet. The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译：提供包括至少富集通道和主电泳流路的集成微流体装置。在目标集成装置中，富集通道和主电泳流路被定位成使得废液通过排放口离开所述主电泳流路。主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。

9. 发明申请

US20040060821A1 Tandem isotachophoresis/zone electrophoresis method and system 有权
公开(公告)号：US20040060821A1
公开(公告)日：2004-04-01
申请号：US10676857
申请日：2003-09-30
申请人： ACLARA BioSciences, Inc.
发明人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
IPC分类号： G01N027/26
CPC分类号： B01L3/5027 , B01L3/502753 , B01L2200/0605 , B01L2300/0816 , B01L2400/0415 , B01L2400/0421 , B01L2400/049 , C07K1/26 , C07K1/28 , G01N27/44773
摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.

10. 发明申请

US20020092767A1 Multiple array microfluidic device units 审中-公开
标题翻译：多阵列微流体装置单元
公开(公告)号：US20020092767A1
公开(公告)日：2002-07-18
申请号：US09995909
申请日：2001-11-28
申请人： ACLARA BioSciences, Inc.
发明人： Torleif Ove Bjornson , Timothy F. Smith
IPC分类号： G01N027/26 , G01N027/447
CPC分类号： B01L3/0241 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00358 , C40B60/14 , G01N35/1065 , G01N35/1074 , G01N2035/1037
摘要： Microfluidic unit arrays and their use are provided for performing in parallel a plurality of operations. The units are arrayed in a format comparable to microtiter well formats, so that transfer by a dispenser having a plurality of dispensing units can be performed with the same footprint, the format of the source and microfluidic unit receiving reservoirs are substantially the same. Operations are carried out simultaneously under comparable conditions, which permits more exact comparisons between the operations.
摘要翻译：微流体单元阵列及其用途被提供用于并行执行多个操作。这些单元以与微量滴定孔格式相当的格式排列，使得具有多个分配单元的分配器的转印可以以相同的占地面积进行，源和微流体单元接收储存器的格式基本相同。操作在可比较的条件下同时进行，这样可以更准确地比较操作。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式