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1. 发明申请

US20040144647A1 Electrophoresis device and the use thereof 失效
标题翻译：电泳装置及其用途
公开(公告)号：US20040144647A1
公开(公告)日：2004-07-29
申请号：US10471984
申请日：2004-03-08
发明人： Wolfgang Dorner , Renate Konrad , Raimund Konrad
IPC分类号： G01N027/27 , G01N027/453
CPC分类号： G01N27/44795 , G01N27/44773
摘要： The invention relates to a device for electrophoretically separating molecules. The inventive device comprises: at least one plate (100) having a top side (200) and an underside (300); a first separating channel (210) provided for receiving at least one first separating medium (220); at least one second channel (310), which is orthogonal to said first separating channel and which is provided for receiving a second separating medium; optionally comprises implements provided for filling the device with reagents, solvents, buffers, separating media and/or for loading the device with a sample to be separated, and; terminals for applying electric separating voltage to the separating channels. In the device, the first separating channel is located on the plate top side and comprises at least one first opening (216) that leads to the plate underside, whereby the second separating channel is located on the plate underside and comprises at least one second opening (316). The first opening of the first channel and the second opening of the second channel are connected to one another.

2. 发明申请

US20040094418A1 Microchannel device 审中-公开
标题翻译：微通道设备
公开(公告)号：US20040094418A1
公开(公告)日：2004-05-20
申请号：US10467839
申请日：2003-12-15
发明人： Timothy Ingram Cox , Mark Christopher Tracey
IPC分类号： G01N027/27 , G01N027/403
CPC分类号： B01F15/0203 , B01F5/0068 , B01F5/0082 , B01F13/0059 , B01F13/0093 , B01F15/024
摘要： This invention relates to a microchannel device comprising a microchannel and a liquid introduction means for introducing at least two liquids into the microchannel; characterised in that the introduction means comprises a pulse means for introducing each liquid into the microchannel in the form of a plurality of pulses, and for staggering the pulses of each liquid relative to the pulses of the other liquids. The device may be used to mix liquids for microfluidic applications.
摘要翻译：本发明涉及一种微通道装置，其包括微通道和用于将至少两种液体引入微通道的液体引入装置; 其特征在于，引入装置包括用于以多个脉冲的形式将每个液体引入微通道的脉冲装置，并且用于相对于其它液体的脉冲交错每个液体的脉冲。该装置可用于混合用于微流体应用的液体。

3. 发明申请

US20040026252A1 Extended pathlength detection in separations 审中-公开
标题翻译：分离中的扩展路径长度检测
公开(公告)号：US20040026252A1
公开(公告)日：2004-02-12
申请号：US10347187
申请日：2003-01-21
发明人： Qingbo Li
IPC分类号： G01N027/27
CPC分类号： G01N27/44721
摘要： The present invention relates to a separation apparatus for the separation and detection of components of a sample. The apparatus includes at least one separation lane suitable for at least partially separating the sample components along a separation direction. A light source configured to emit light suitable to interact with the sample components is disposed in optical communication with the separation lane. Light from the light source enters the separation lane at a light entry point therealong. At least some of the light that enters the separation lane exits the separation lane at a light exit point. The light exit point is spaced apart from the light entry point along the separation direction. A light detector detects the light exiting the separation lane.
摘要翻译：本发明涉及用于分离和检测样品组分的分离装置。该装置包括至少一个适于沿着分离方向至少部分地分离样品组分的分离通道。被配置为发射适合于与样品组分相互作用的光的光源被设置成与分离通道光学连通。来自光源的光在其入口点进入分离通道。进入分离通道的至少一些光在光出口处离开分离通道。光出射点沿着分离方向与光入射点间隔开。光检测器检测离开分离通道的光。

4. 发明申请

US20040011649A1 Method and device for injecting a sample in an electrophoresis capillary 审中-公开
标题翻译：用于在电泳毛细管中注射样品的方法和装置
公开(公告)号：US20040011649A1
公开(公告)日：2004-01-22
申请号：US10276079
申请日：2003-04-07
发明人： Gilbert Gauguet , Rainer Siebert
IPC分类号： G01N027/27
CPC分类号： G01N27/44743
摘要： A method of injecting a sample into an electrophoresis capillary, in which method the capillary (1) is plunged into the sample and an electric field is applied between the ends of the capillary (1) to cause the sample to migrate into the capillary (1), the method being characterized in that the sample is previously introduced into a part (3) presenting a channel (4) of dimensions perpendicular to the direction in which said channel (4) extends that are smaller than about four times the outside diameter of the capillary (1), and in that in order to plunge the capillary (1) into the sample, said capillary (1) is introduced into said channel (4).
摘要翻译：将样品注入电泳毛细管的方法，其中毛细管（1）插入样品中，并且在毛细管（1）的端部之间施加电场以使样品迁移到毛细管（1）中），所述方法的特征在于，样品预先引入到具有垂直于所述通道（4）延伸的方向的尺寸的通道（4）的部分（3）中，所述通道（4）的尺寸小于所述通道（4）延伸的方向的大约的四倍毛细管（1），并且为了将毛细管（1）插入样品中，所述毛细管（1）被引入所述通道（4）中。

5. 发明申请

US20030217925A1 Electrophoresis gel identifier and method of making and using the same 审中-公开
标题翻译：电泳凝胶标识符及其制作和使用方法
公开(公告)号：US20030217925A1
公开(公告)日：2003-11-27
申请号：US10414940
申请日：2003-04-15
申请人： PageGel, Inc.
发明人： William F. Alpenfels , Anthony Paul DiScuillo , Gary Lee Ousley
IPC分类号： G01N027/27 , G01N027/403
CPC分类号： G01N27/44704
摘要： A method of creating an electrophoresis gel having a gel electrophoresis identifier includes providing a surface including a gel electrophoresis identifier marker; providing an electrophoresis gel on the surface having the gel electrophoresis identifier marker; and forming a gel electrophoresis identifier on the electrophoresis gel with the gel electrophoresis identifier marker of the surface. The gel electrophoresis identifier is used to identify and orient the gel.
摘要翻译：产生具有凝胶电泳标识物的电泳凝胶的方法包括提供包括凝胶电泳标识符标记的表面; 在具有凝胶电泳识别标记的表面上提供电泳凝胶; 并用表面的凝胶电泳标识符标记在电泳凝胶上形成凝胶电泳标识物。凝胶电泳标识符用于鉴定和定向凝胶。

6. 发明申请

US20030150727A1 Monster capillary array electrophoresis scanner 有权
标题翻译：怪物毛细管阵列电泳扫描仪
公开(公告)号：US20030150727A1
公开(公告)日：2003-08-14
申请号：US10285290
申请日：2002-10-31
申请人： Affymetrix, Inc.
发明人： Richard A. Mathies , James R. Scherer
IPC分类号： G01N027/27
CPC分类号： G01N27/44721 , G01N27/44782
摘要： An improved rotary confocal fluorescence scanner capable of detecting analytes separated on over a 1,000 capillaries simultaneously. This system uses a confocal microscope objective and mirror assembly that rotates inside a vertical ring of capillaries to provide rapid and efficient excitation and detection of fluorescently labeled fragments separated within a cylindrical capillary array. Use of automated procedures to load and run all capillaries permits one to read more than 350,000 base pairs of raw sequence data per hour.
摘要翻译：改进的旋转共聚焦荧光扫描仪能够同时检测超过1,000个毛细管分离的分析物。该系统使用在垂直的毛细管环内旋转的共焦显微镜物镜和镜组件，以提供在圆柱形毛细管阵列内分离的荧光标记片段的快速和有效的激发和检测。使用自动化程序加载和运行所有毛细管，可以读取每小时超过35万个碱基对原始序列数据。

7. 发明申请

US20030116437A1 Electrophoresis in microfabricated devices using photopolymerized polyacrylamide gels and electrode-defined sample injection 失效
标题翻译：使用光聚合聚丙烯酰胺凝胶和电极定义的样品注射在微制造装置中进行电泳
公开(公告)号：US20030116437A1
公开(公告)日：2003-06-26
申请号：US10277348
申请日：2002-10-22
申请人： The Regents Of The University Of Michigan
发明人： Mark A. Burns , Sundaresh N. Brahmasandra , Victor M. Ugaz
IPC分类号： G01N027/453 , G01N027/27
CPC分类号： G01N27/44743 , G01N27/44704 , G01N27/44791
摘要： The present invention relates to a novel, small-scale, electrophoretic separation system based on photodefined polymers and electrode-defined sample injection. Diffusion and displacement coefficients may be modified by varying the gel concentration, the intensity of the incident UV radiation and the temperature at which the gel is run. The device is an major advance over current technology since it provides for a significant reduction in size of the micro-electrophoresis apparatus and a significant cost savings.
摘要翻译：本发明涉及一种基于光定义聚合物和电极定义样品注入的新型小规模电泳分离系统。可以通过改变凝胶浓度，入射的UV辐射的强度和凝胶运行的温度来改变扩散和置换系数。该设备是超过现有技术的重大进步，因为它提供了显着减小微电泳装置的尺寸并显着降低了成本。

8. 发明申请

US20020012910A1 AUTOMATED HYBRIDIZATION/IMAGING DEVICE FOR FLUORESCENT MULTIPLEX DNA SEQUENCING 无效
标题翻译：用于荧光多重DNA序列的自动化混合/成像装置
公开(公告)号：US20020012910A1
公开(公告)日：2002-01-31
申请号：US08563462
申请日：1995-11-28
发明人： ROBERT B. WEISS , ALVIN W. KIMBALL , RAYMOND F. GESTELAND , F. MARK FERGUSON , DIANE M. DUNN , LEONARD J. DI SERA , JOSHUA L. CHERRY
IPC分类号： C12Q001/68 , G01N021/00 , G01N031/00 , G01N033/00 , C12P019/34 , C12M001/38 , C12M003/00 , G01N027/453 , C12M001/36 , C12M001/34 , G01R001/00 , C25B013/00 , G01N027/27 , G01N027/403 , G02B013/18 , G02B003/06 , C25B011/00 , C02F011/00 , C25B009/00 , C02F001/40
CPC分类号： G01N27/44721 , B01L7/52 , C12Q1/6869
摘要： A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots.
摘要翻译：公开了一种利用集成的自动成像杂交室系统对DNA进行自动多重测序的方法。该系统包括用于安装含有尺寸分级的多重测序反应产物的膜的杂交室装置，用于流体输送到室装置的装置，用于光传输到膜的成像装置和在室中从膜发出的荧光的图像记录设备和可编程控制器装置，用于控制系统的操作。多重反应产物与探针杂交，然后将酶（例如碱性磷酸酶）结合到探针上的结合部分，并且通过流体递送将荧光底物（例如苯并噻唑衍生物）引入腔室装置仪器。该酶将荧光底物转化为荧光产品，当在来自成像装置的光束在腔室装置中照射时，荧光产物激发荧光产物的荧光以产生杂交图谱。通过成像装置的CCD照相机部件对杂交图案进行成像，以获得一系列数字信号。这些信号由控制器装置转换为与核苷酸序列相对应的核苷酸序列的一串自动序列读取器。该方法和装置也适用于其他基于膜的应用，例如菌落和斑块杂交以及Southern，Northern和Western印迹。

9. 发明申请

US20010045358A1 Ultra high throughput microfluidic analytical systems and methods 有权
标题翻译：超高通量微流体分析系统和方法
公开(公告)号：US20010045358A1
公开(公告)日：2001-11-29
申请号：US09920090
申请日：2001-07-31
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael Kennedy
IPC分类号： G01N027/26 , G01N027/447 , G01N027/453 , G01N027/27 , G01N021/85
CPC分类号： G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
摘要翻译：分析系统和方法使用模块化接口结构来提供样品基底和分析单元之间的界面，其中分析单元通常具有用于实现各种分析和控制功能的特定界面布置。使用模块化接口结构的每个模块的许多变体有利地提供了使用具有特定分析和控制系统接口布置的特定基板或类别的基板执行大量测试的成本有效和有效的方式。还提供了用于同时分析多个并行微通道中的反应或条件的改进的光学照明和检测系统。通过使用包括多个激发频率的激发束同时照射非法线入射的多个平行微通道并且同时在垂直于衬底的方向上检测来自微通道中的物质的发射，本发明提供了增加的吞吐量和改进的排放物检测使用具有多个检测器的检测模块。

10. 发明申请

US20010035352A1 Toner characterization cell 失效
标题翻译：墨粉表征细胞
公开(公告)号：US20010035352A1
公开(公告)日：2001-11-01
申请号：US09771487
申请日：2001-01-25
发明人： Alexander Borisovich Ozerov
IPC分类号： G01N027/27 , G01N027/26
CPC分类号： G01N33/32 , G01N27/447
摘要： A toner characterization cell used to determine characteristics of insoluble particles in a liquid medium. First and second electrode are spaced apart and an electric field is applied between the electrodes and a displacement current is measured. Optical density measuring devices measure the change in optical density in the cell adjacent each electrode. The characteristics are determined from the displacement current and the change in optical density. The characterizing feature may be particle mobility. The invention also relates to a method of determining the characteristics.
摘要翻译：用于确定液体介质中不溶性颗粒特性的调色剂表征池。第一和第二电极间隔开，并且在电极之间施加电场并测量位移电流。光密度测量装置测量每个电极附近的电池中光密度的变化。特性由位移电流和光密度的变化决定。特征特征可以是粒子移动性。本发明还涉及一种确定特征的方法。

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