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1. 发明授权

US06916612B2 Sets of oligonucleotide-binding e-tag probes 失效
标题翻译：套寡核苷酸结合电子标签探针
公开(公告)号：US06916612B2
公开(公告)日：2005-07-12
申请号：US09825246
申请日：2001-04-02
申请人： Sharat Singh , Tracy Matray , Ahmed Chenna
发明人： Sharat Singh , Tracy Matray , Ahmed Chenna
IPC分类号： C12N15/09 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/68 , B01D57/02 , C07H21/04
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C40B70/00 , G01N33/561 , Y10S435/81
摘要： Probe sets for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or “e-tag probes”, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了用于已知选择的核苷酸靶序列的多路检测的探针组。检测涉及到识别标签作为目标识别的结果。探针组包括电泳标签探针或“e-标签探针”，其包含与靶结合部分连接的检测区域和称为迁移率调节剂的迁移率限定区域。 e-标签探针的靶结合部分与互补靶序列杂交，随后是e-标签探针的核酸酶切割和可检测的电子标签或电子标签记录的释放。将混合物暴露于捕获剂，其结合未切割和/或部分切割的e-标签探针，然后进行电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

2. 发明申请

US20050048553A1 Multiplexed analysis by chromatographic separation of molecular tags 审中-公开
标题翻译：通过色谱分离分子标签的多重分析
公开(公告)号：US20050048553A1
公开(公告)日：2005-03-03
申请号：US10918876
申请日：2004-08-16
申请人： Ahmed Chenna , Tracy Matray , Vincent Hernandez , Herbert Hooper , Sharat Singh
发明人： Ahmed Chenna , Tracy Matray , Vincent Hernandez , Herbert Hooper , Sharat Singh
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/58 , C12Q1/6809 , G01N33/6845 , G01N2030/027 , C12Q2565/102 , C12Q2565/137
摘要： Methods and kits are disclosed for determining, either in a homogeneous or heterogeneous assay format, one or more target analytes in a sample using binding compositions coupled to molecular tags by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such binding compositions under conditions that permit stable complexes to form between the binding compositions and analytes. In one aspect of the invention, the interaction between the binding compositions and their respective binding sites brings a cleavage-inducing moiety into close proximity to cleavable linkages or provides a recognizable substrate for a cleavage-inducing moiety. In this way, one or more molecular tags for each of the analytes are released from the complexes. Released molecular tags are chromatographically separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.
摘要翻译：公开了用于以同质或非均相测定形式测定样品中一种或多种目标分析物的方法和试剂盒，其使用通过可切割键连接分子标签的结合组合物。通常，在允许在结合组合物和分析物之间形成稳定的复合物的条件下，形成包含样品和包含多种这样的结合组合物的试剂的测定混合物。在本发明的一个方面，结合组合物和它们各自的结合位点之间的相互作用使切割诱导部分靠近可裂解连接，或为切割诱导部分提供可识别的底物。以这种方式，每个分析物的一个或多个分子标签从配合物中释放出来。释放的分子标签被色谱分离，并且基于释放和分离的分子标签的分析确定靶分析物的存在和/或量。

3. 发明授权

US06770439B2 Sets of generalized target-binding e-tag probes 有权
标题翻译：一套广泛的目标绑定电子标签探针
公开(公告)号：US06770439B2
公开(公告)日：2004-08-03
申请号：US09825244
申请日：2001-04-02
申请人： Sharat Singh , Tracy Matray , Ahmed Chenna
发明人： Sharat Singh , Tracy Matray , Ahmed Chenna
IPC分类号： C12Q168
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C40B70/00 , G01N33/561 , Y10S435/81
摘要： Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。检测涉及到识别标签作为目标识别的结果。探针组包括电泳标签探针或e-标签探针，其包含检测区域和称为迁移率调节剂的迁移率限定区域，两者都连接到靶结合部分。将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

4. 发明授权

US07001725B2 Kits employing generalized target-binding e-tag probes 失效
标题翻译：套件采用广义目标绑定电子标签探针
公开(公告)号：US07001725B2
公开(公告)日：2006-02-21
申请号：US09824851
申请日：2001-04-02
申请人： Sharat Singh , Tracy Matray , Ahmed Chenna
发明人： Sharat Singh , Tracy Matray , Ahmed Chenna
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , B65D85/42 , G01N27/26
CPC分类号： C07H19/10 , C07H19/06 , C07H21/00 , Y10S435/808
摘要： Kits for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, a capture agent and optionally a cleaving agent. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了用于多个检测一种或多种配体和靶抗配糖体的结合或相互作用的试剂盒。检测涉及到识别标签作为目标识别的结果。试剂盒包括电泳标签探针或电子标签探针，捕获剂和任选的裂解剂。电子标签探针包括检测区域和称为迁移率调节剂的迁移率限定区域，两者都与靶结合部分连接。在使用试剂盒时，将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

5. 发明授权

US06955874B2 Kits employing oligonucleotide-binding e-tag probes 失效
标题翻译：使用寡核苷酸结合电子标签探针的试剂盒
公开(公告)号：US06955874B2
公开(公告)日：2005-10-18
申请号：US09824905
申请日：2001-04-02
申请人： Sharat Singh , Tracy Matray , Ahmed Chenna
发明人： Sharat Singh , Tracy Matray , Ahmed Chenna
IPC分类号： C12N15/09 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/68 , B65D69/00 , C07H21/02 , C07H21/04 , G01H27/26
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C40B70/00 , G01N33/561 , Y10S435/81
摘要： Kits for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, capture agent and optionally a nuclease. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, the target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了用于已知选择的核苷酸靶序列的多重检测的试剂盒。检测涉及到识别标签作为目标识别的结果。该试剂盒包括一组电泳标签探针或电子标签探针，捕获剂和任选的核酸酶。电子标签探针包括检测区域和称为迁移率调节剂的迁移率限定区域，两者都与靶结合部分连接。在使用试剂盒时，e-标签探针的靶结合部分与互补的靶序列杂交，随后是e-标签探针的核酸酶切割和可检测的电子标签或电子标签记录的释放。将混合物暴露于捕获剂，其结合未切割和/或部分切割的e-标签探针，然后进行电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

6. 发明授权

US07037654B2 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents 失效
标题翻译：用于增强使用可切割电泳标签试剂的测定中检测的方法和组合物
公开(公告)号：US07037654B2
公开(公告)日：2006-05-02
申请号：US10011201
申请日：2001-11-09
申请人： Ahmed Chenna , Sharat Singh
发明人： Ahmed Chenna , Sharat Singh
IPC分类号： C12Q1/68 , C07K7/00 , A23J1/00 , C07H21/04 , C25D17/00
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/6818 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00 , G01N33/561 , C12Q2521/319 , C12Q2563/131 , C12Q2565/30
摘要： Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。检测涉及到识别标签作为目标识别的结果。探针组包括电泳标签探针或e-标签探针，其包含检测区域和称为迁移率调节剂的迁移率限定区域，两者都连接到靶结合部分。这些探针包括与位于探针中的切割部分相邻的交互功能，使得交互功能不构成电子标签记录器的一部分。将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

7. 发明授权

US06632630B2 Monooxygenase assays 失效
标题翻译：单加氧酶测定
公开(公告)号：US06632630B2
公开(公告)日：2003-10-14
申请号：US09820289
申请日：2001-03-28
申请人： Sharat Singh , Ahmed Chenna , Hossein Salimi-Moosari , Ian Gibbons
发明人： Sharat Singh , Ahmed Chenna , Hossein Salimi-Moosari , Ian Gibbons
IPC分类号： C12Q126
CPC分类号： C12Q1/26 , G01N2333/795 , G01N2333/80 , G01N2333/90245 , Y10S435/968
摘要： Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.
摘要翻译：使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒，其特征在于具有氧化成醛的可氧化亚甲基和荧光肼。向反应混合物中加入荧光肼，通过毛细管电泳分析得到的腙。该方法可用于评价化合物的酶调节活性

8. 发明授权

US06673550B2 Electrophoretic tag reagents comprising fluorescent compounds 有权
标题翻译：包含荧光化合物的电泳标签试剂
公开(公告)号：US06673550B2
公开(公告)日：2004-01-06
申请号：US10008495
申请日：2001-11-09
申请人： Tracy Matray , Vincent Hernandez , Sharat Singh
发明人： Tracy Matray , Vincent Hernandez , Sharat Singh
IPC分类号： C12Q168
CPC分类号： C07H21/04 , C07D311/82 , C07H19/073 , C07H19/10 , C07H19/173 , C12Q1/6823 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00 , G01N33/561 , G01N33/58 , G01N33/6845 , C12Q2563/167
摘要： Electrophoretic probes comprising fluorescent compounds as detection groups and mobility modifiers are disclosed for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. In one embodiment, detection involves the release of identifying tags as a consequence of target recognition. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters. Typically, uncleaved or partially cleaved e-tag probes are removed and the mixture of e-tag reporters is separated by any technique that provides for separation by mass or mass to charge ratio and the like and detected to provide for target identification.
摘要翻译：公开了包含作为检测基团的荧光化合物和迁移率调节剂的电泳探针，用于多个检测一种或多种配体和靶抗配液体的结合或相互作用。在一个实施例中，检测涉及作为目标识别的结果的识别标签的释放。将目标抗配糖与一组电子标签探针接触，并用选定的裂解剂处理接触的抗配糖剂，导致电子标签记录器的混合。通常，去除未切割或部分切割的电子标签探针，并且通过任何通过质量或质量与电荷比等进行分离并且被检测以提供靶标鉴定的技术分离电子标签报告物的混合物。

9. 发明申请

US20050079565A1 Detecting and profiling molecular complexes 有权
标题翻译：检测和分析分子复合物
公开(公告)号：US20050079565A1
公开(公告)日：2005-04-14
申请号：US10916291
申请日：2004-08-10
申请人： Po-Ying Chan-Hui , Sharat Singh , Hossein Salimi-Moosavi , Hasan Tahir , Gerald Wallweber , Hrair Kirakosssian , Tracy Matray
发明人： Po-Ying Chan-Hui , Sharat Singh , Hossein Salimi-Moosavi , Hasan Tahir , Gerald Wallweber , Hrair Kirakosssian , Tracy Matray
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/537 , G01N33/542 , G01N33/543 , G01N33/566
CPC分类号： G01N33/542 , G01N33/566 , G01N2333/71 , G01N2333/726 , Y10S436/824
摘要： Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.
摘要翻译：提供了用于检测样品（例如细胞或组织裂解物）中分子复合物（特别是蛋白质）的形成的方法。在一个方面，提供了对复合物中的第一种蛋白质特异性的切割探针和一种或多种特异于复合物中的一种或多种第二种蛋白质的结合化合物。在结合时，诱导切割探针以产生仅在切割探针的局部区域中切割附着于结合化合物的分子标签的活性物质，例如单线态氧。将释放的分子标签与测定混合物和彼此分离，以提供与复合物中存在的蛋白质的数量和类型相关的读数。

10. 发明授权

US06627400B1 Multiplexed measurement of membrane protein populations 有权
标题翻译：膜蛋白种群的多重测量
公开(公告)号：US06627400B1
公开(公告)日：2003-09-30
申请号：US09698846
申请日：2000-10-27
申请人： Sharat Singh , Tracy Matray
发明人： Sharat Singh , Tracy Matray
IPC分类号： C12Q168
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00
摘要： Families of compositions are provided as labels, referred to as eTag reporters for attaching to polymeric compounds and assaying based on release of the eTag reporters from the polymeric compound and separation and detection. For oligonucleotides, the eTag reporters are synthesized at the end of the oligonucleotide by using phosphite or phosphate chemistry, whereby mass-modifying regions, charge-modifying regions and detectable regions are added sequentially to produce the eTag labeled reporters. By using small building blocks and varying their combination large numbers of different eTag reporters can be readily produced attached to a binding compound specific for the target compound of interest for identification. Protocols are used that release the eTag reporter when the target compound is present in the sample.
摘要翻译：提供组合物家族作为标记，被称为eTag报告基因，用于附着于聚合物上，并基于从高分子化合物释放eTag报告物并进行分离和检测。对于寡核苷酸，通过使用亚磷酸酯或磷酸酯化学在寡核苷酸的末端合成eTag报告物，由此顺序加入质量修饰区域，电荷修饰区域和可检测区域以产生eTag标记的记录。通过使用小的构建块并改变它们的组合，可以容易地产生大量不同的eTag报告物，其连接到目的化合物特异性的结合化合物用于鉴定。当目标化合物存在于样品中时，使用释放eTag报告物的方案。

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