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71. 发明授权

US4446230A Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae 失效
标题翻译：淋病实验室诊断试验方法和淋病奈瑟氏球菌检测菌株
公开(公告)号：US4446230A
公开(公告)日：1984-05-01
申请号：US317023
申请日：1981-10-30
申请人： Leonard J. Zubrzycki
发明人： Leonard J. Zubrzycki
IPC分类号： A61B10/00 , A61B10/02 , C12N1/20 , C12N1/21 , C12Q1/04 , C12Q1/12 , C12R1/36 , C12Q1/68
CPC分类号： C12R1/36 , C12N1/20 , C12Q1/04 , C12Q1/12 , G01N2333/22 , Y10S435/871
摘要： A strain of Neisseria gonorrhoeae ATCC 31953 is described which is abnormal in that it has characteristically poor growth on chocolate agar at a temperature range of about 30.degree. C. to about 37.degree. C. in a CO.sub.2 atmosphere suitable for growth of N. gonorrhoeae. This strain is resistant to nalidixic acid at the 5-10 mcg/ml level and resistant to streptomycin at the 1000 mcg/ml level or greater. N. gonorrhoeae ATCC 31953 is a test strain suitable for use in the method described for the laboratory diagnosis of gonorrhea. The method comprises the steps of (1) applying a non-toxic preparation of a patient's specimen material, directly to a culture of Neisseria gonorrhoeae ATCC 31953, which has abnormal growth characteristics, which is in or on a biological medium suitable for growth of normal Neisseria gonorrhoeae, and observing for the restoration of normal growth to the abnormal growth strain Neisseria gonorrhorae ATCC 31953, in or on the biological medium of step (1), under conditions normal for growth of Neisseria gonorrhoeae. The observence of growth indicates positive detection of N. gonorrhoeae DNA.
摘要翻译：描述了淋病奈瑟氏球菌ATCC 31953的菌株，其特征在于在适合于淋病奈瑟氏球菌生长的CO 2气氛中，在约30℃至约37℃的温度范围内巧克力琼脂的特征性差的生长。该菌株对5-10mcg / ml水平的萘啶酸耐受，在1000mcg / ml或更高水平下耐链霉素。淋病奈瑟氏球菌ATCC 31953是适合用于实验室诊断淋病的方法中使用的测试菌株。该方法包括以下步骤：（1）将患者标本材料的无毒制剂直接施用于具有异常生长特征的淋病奈瑟氏球菌ATCC 31953的培养物，其在适于正常生长的生物培养基中或其上淋病奈瑟氏球菌，并且在步骤（1）的生物培养基中或其上，在淋病奈瑟氏球菌生长条件下观察恢复正常生长至异常生长菌株淋病奈瑟氏球菌ATCC 31953。生长的观察表明淋病奈瑟氏球菌DNA阳性检测。

72. 发明授权

US4386066A Immunogenic complex from N. gonorrhoeae 失效
标题翻译：来自淋病奈瑟氏球菌的免疫原性复合物
公开(公告)号：US4386066A
公开(公告)日：1983-05-31
申请号：US294855
申请日：1981-08-20
申请人： Edward C. O'Rourke , W. Wardle Fullerton
发明人： Edward C. O'Rourke , W. Wardle Fullerton
IPC分类号： A61K39/095 , C12N1/20 , C12P21/00
CPC分类号： A61K39/095 , Y10S435/871
摘要： A vesicular, immunogenic, non-pyrogenic complex is obtained from isolates of N. gonorrhoeae by cultivating N. gonorrhoeae on a suitable nutrient medium; harvesting and centrifuging the cells; extracting the cell pellet with TRITON X-100 at alkaline pH; suspending the solids remaining after extraction in aqueous medium and precipitating in the cold with ethanol.
摘要翻译：通过在合适的营养培养基上培养淋病奈瑟氏球菌从淋病奈瑟氏球菌的分离株获得泡状，免疫原性，非致热原复合物; 收获和离心细胞; 在碱性pH下用TRITON X-100萃取细胞沉淀; 悬浮在水介质中提取后残留的固体，并在乙醇中冷却沉淀。

73. 发明授权

US4070249A Apparatus for compensating for pressure within a biological test device 失效
标题翻译：用于补偿生物测试装置内的压力的装置
公开(公告)号：US4070249A
公开(公告)日：1978-01-24
申请号：US693836
申请日：1976-06-08
申请人： Pierre R. Janin , Holger Hagen
发明人： Pierre R. Janin , Holger Hagen
IPC分类号： G01N37/00 , C12K1/04
CPC分类号： B01L3/502 , B01L2300/042 , B01L2300/048 , B01L2300/0663 , B01L2300/0832 , B01L2300/0854 , B01L2300/0864 , B01L2300/14 , B01L2400/0457 , B01L3/50825 , Y10S435/822 , Y10S435/849 , Y10S435/871 , Y10S435/873 , Y10S435/88
摘要： A biological testing device comprising a container and plug is disclosed. The container in combination with the plug makes it possible to test a bacterial suspension with a number of different reagents simultaneously. The container which is open at one end has a plurality of microtubes arranged around its lower inside periphery. The plug which fits within the opening of the container distributes the bacterial suspension in substantially equal amounts to each of the microtubes having different testing reagents. A compression compensator adapted for use with the biological testing device is also disclosed. The compensator comprises a chamber which is in communication with the interior of the container when the plug is in place therein. The chamber contains a disc therein which can move in the manner of a piston to increase the volume of the chamber and thereby receive any gas or vapor vented from the testing device during use.
摘要翻译：公开了一种包括容器和塞子的生物测试装置。容器与塞子组合使得可以同时测试具有多种不同试剂的细菌悬浮液。在一端开口的容器具有围绕其下内周布置的多个微管。适合于容器开口内的塞子以相当数量的细菌悬浮液分配具有不同测试试剂的每个微管。还公开了适用于生物测试装置的压缩补偿器。补偿器包括当塞子在其中就位时与容器的内部连通的腔室。该室包含其中可以以活塞的方式移动的盘，以增加室的体积，从而接收在使用期间从测试装置排出的任何气体或蒸气。

74. 发明授权

US4053363A Sputum analysis method 失效
标题翻译： SPUTUM分析方法
公开(公告)号：US4053363A
公开(公告)日：1977-10-11
申请号：US700215
申请日：1976-06-28
申请人： Gordon L. Dorn
发明人： Gordon L. Dorn
IPC分类号： G01N33/48 , B01F17/56 , C12Q1/04 , G01N33/50 , C12K1/00 , G01N33/16
CPC分类号： C12Q1/04 , Y10S435/822 , Y10S435/842 , Y10S435/849 , Y10S435/852 , Y10S435/87 , Y10S435/871 , Y10S435/873 , Y10S435/874 , Y10S435/879 , Y10S435/882 , Y10S435/885 , Y10S435/921 , Y10S435/944
摘要： An in vitro diagnostic technique for quantitative analysis of human sputum, such as lung fluid, for the presence of microbial pathogens is provided wherein a sputum sample is contacted with a minor quantity of nontoxic saponin to degrade the viscosity of the sputum and thereafter the sputum is thoroughly admixed to distribute microbial pathogens generally uniformly therein. The sputum can be diluted as desired and then subjected to conventional analytical techniques, i.e., plating on growth media and recording results.

75. 发明授权

US4038143A Test kit for the genetic detection of microorganisms 失效
标题翻译：微生物遗传检测试剂盒
公开(公告)号：US4038143A
公开(公告)日：1977-07-26
申请号：US605059
申请日：1975-08-15
申请人： Elliot Juni
发明人： Elliot Juni
IPC分类号： C12N15/00 , C12Q1/04 , C12Q1/12 , C12Q1/14 , C12Q1/68 , C12K1/10
CPC分类号： C12Q1/6888 , C12N15/00 , C12Q1/04 , C12Q1/12 , C12Q1/14 , C12Q1/6806 , Y10S435/822 , Y10S435/832 , Y10S435/851 , Y10S435/871 , Y10S435/882 , Y10S435/885
摘要： A test kit for detecting the presence of a specific microorganism in a biological sample utilizing the phenomenon of genetic transformation. The test kit comprises a transformable variant of said specific microorganism, such as auxotrophs which are distinguishable from their wild parent strain. The variants are transformed by deoxyribonucleic acid (DNA) extracted from microorganism cells present in a biological test sample. The detection of transformed cells indicates the presence of the specific microorganism under determination in the sample. The microorganism cells present in the biological sample are lysed utilizing an aqueous solution containing a surfactant capable of performing the lysing step.

76. 发明授权

US4017420A Stable oxidase reagent solutions 失效
标题翻译：稳定的氧化酶试剂溶液
公开(公告)号：US4017420A
公开(公告)日：1977-04-12
申请号：US643465
申请日：1975-12-22
申请人： Betty Anne Bowie , Joseph F. Pagano
发明人： Betty Anne Bowie , Joseph F. Pagano
IPC分类号： C12Q1/12 , C12Q1/04 , C12Q1/26 , C09K3/00
CPC分类号： C12Q1/26 , C12Q1/04 , Y10S435/871
摘要： Stable oxidase reagent solutions containing N,N,N',N'-tetramethyl-p-phenylenediamine or N,N-dimethyl-p-phenylenediamine for determining the presence of oxidase in microbial colonies.
摘要翻译：含有N，N，N'，N'-四甲基对苯二胺或N，N-二甲基对苯二胺的稳定氧化酶试剂溶液，用于测定微生物菌落中氧化酶的存在。

77. 发明授权

US3936355A Microorganism growth media and the stabilization thereof 失效
标题翻译：微生物生长培养基及其稳定性
公开(公告)号：US3936355A
公开(公告)日：1976-02-03
申请号：US415013
申请日：1973-11-12
申请人： John W. Lawson
发明人： John W. Lawson
IPC分类号： C12Q1/12 , C12K1/10
CPC分类号： C12Q1/12 , Y10S435/871
摘要： Media for supporting the growth of certain types of microorganisims, especially Neisseria gonorrhoeae and Neisseria meningitidis, are described. These media, when supplemented with polyvinylpyrrolidone (PVP), albumin and penicillin induce microorganism transformation to the wall-defective or "L-form." The PVP is necessarily "detoxified" for use in the L-form inducing media.
摘要翻译：描述了用于支持某些类型的微生物，特别是淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌的生长的培养基。当补充有聚乙烯吡咯烷酮（PVP），白蛋白和青霉素时，这些培养基诱导微生物转化为壁缺陷或“L型”。 PVP必须“解毒”才能用于L型诱导培养基。

78. 发明授权

US3902971A Biological detecting method and apparatus 失效
标题翻译：生物检测方法和装置
公开(公告)号：US3902971A
公开(公告)日：1975-09-02
申请号：US45952665
申请日：1965-05-20
申请人： AKZONA INC
发明人： FLETCHER JAMES C , HENDRIX CHARLES E , KILNER SCOTT B , LEHAN FRANK W , MAGILL PAUL L , MISHUCK ELI , PERKINS JR WILLIAM A , ROBINSON MERTON A , SWEENY KEITH H , TEW RICHARD W
IPC分类号： G01N33/569 , C12K1/04
CPC分类号： G01N33/569 , Y10S435/866 , Y10S435/871
摘要： This disclosure describes apparatus and the method for continuously sampling either gaseous or liquid media such as atmosphere or water to detect the presence or absence of specific pathogenic micro-organisms. The system as described employs collection apparatus which entrains particles of microorganism size in continuous flowing stream of water. A suspension of fluorescently tagged antibodies is injected into the flowing stream and thereby maintained in contact for a sufficient period of time for the specific antibodies to attach to any of its corresponding organism in the collection stream. The resultant mixture of the two streams is passed through a continuous filter having pore size selected to hold on its surface reacted antigenantibody complexes while passing the remainder of the affluent. After washing to remove extraneous residue, the surface of the filter is exposed to light of a wavelength which excites the fluorescently tagged antibodies if resident on the tape, and the number of such fluorescent particles appearing in the field of a scanned field is counted. In the absence of any fluorescent particles, the original collection stream is known to be free of the particular microorganism. The disclosure describes specific antibody-antigen complexes and their recommended fluorescent dye and exciting wavelength. A wide band preamplifier for detecting the signals on the system is similarly disclosed.
摘要翻译：本公开描述了用于连续取样气体或液体介质如大气或水的装置和方法，以检测特定致病微生物的存在或不存在。所描述的系统使用收集装置，其将连续流动的水中的微生物尺寸的颗粒夹带。将荧光标记的抗体的悬浮液注入流动流中，从而保持接触足够的时间以使特异性抗体附着于收集流中任何相应的生物体。将得到的两股物流混合物通过连续过滤器，该过滤器的孔径选择为保持其表面上反应的抗原 - 抗体复合物，同时通过富余物的其余部分。洗涤除去外来残留物后，将过滤器的表面暴露于激发荧光标记的抗体的波长的光，如果驻留在带上，并且计数在扫描场的场中出现的这种荧光颗粒的数量。在没有任何荧光颗粒的情况下，已知原始收集流不含特定微生物。本公开描述了特异性抗体 - 抗原复合物及其推荐的荧光染料和激发波长。类似地公开了用于检测系统上的信号的宽带前置放大器。

79. 发明授权

US3891508A Method of rapid identification of gram-negative bacteria 失效
标题翻译：快速鉴定革兰氏阴性细菌的方法
公开(公告)号：US3891508A
公开(公告)日：1975-06-24
申请号：US36630373
申请日：1973-06-01
申请人： MERRICK JOSEPH M
发明人： MERRICK JOSEPH M
IPC分类号： C12Q1/04 , C12K1/04
CPC分类号： C12Q1/04 , G01N2333/255 , G01N2400/50 , Y10S435/803 , Y10S435/822 , Y10S435/828 , Y10S435/848 , Y10S435/851 , Y10S435/852 , Y10S435/871 , Y10S435/873 , Y10S435/874 , Y10S435/879
摘要： A method of rapid identification of Gram-negative bacteria by the quantitative and qualitative characterization of the sugars in the lipopolysaccharide (LPS) component of the bacterial cell wall. The invention comprises the procedures of bacterial cultivation, harvesting, LPS extraction, purification, and hydrolysis, and characterization of the sugar components of the LPS by chromatographic analysis.
摘要翻译：通过细菌细胞壁的脂多糖（LPS）组分中的糖的定量和定性表征来快速鉴定革兰氏阴性细菌的方法。本发明包括细菌培养，收获，LPS提取，纯化和水解的步骤，以及通过色谱分析表征LPS的糖组分。

80. 发明授权

US3876503A Method and instrument for the detection of neisseria gonorrheae without culture 失效
标题翻译：用于检测没有文化的NEISSERIA GONORRHEAE的方法和仪器
公开(公告)号：US3876503A
公开(公告)日：1975-04-08
申请号：US19373971
申请日：1971-10-29
申请人： MENNEN FREDERICK C
发明人： MENNEN FREDERICK C
IPC分类号： A61B10/00 , A61B10/02 , C12M1/24 , C12M1/30 , C12Q1/12 , C12K1/04
CPC分类号： C12Q1/12 , A61B10/0096 , A61B10/0291 , A61B2010/0006 , B01L3/5029 , B01L2200/10 , B01L2200/16 , B01L2300/069 , B01L2300/0809 , Y10S435/81 , Y10S435/871
摘要： A non-culture method and instrument for a rapid presumptive test for gonorrhea in which a specimen of exudate from a suspected case is placed in direct contact with a pledget containing a compound which reacts with Neisseria gonorrheae to produce a color change. The pledget, before the test, is in a dry condition and is activated by a wetting agent such as saline, when placed in contact with the pledget. The chemical compound used and incorporated into the pledget is selected from a group consisting of phenylenediamines. The instrument used consists of a flexible tube containing a fluid-filled frangible ampul at one end and the chemically impregnated pledget disposed immediately above same. A separate sterile swab is supplied for taking the specimen. The swab containing the specimen is inserted into the flexible tube. The flexible tube is then squeezed at the ampul portion, releasing the wetting agent by breaking the frangible ampul. The wetting agent when thus released activates the reagents in the pledget. A downward pressure of the swab containing the specimen places it in direct contact with the activated pledget. If gonorrhea is present in the specimen on the swab, a distinctive coloration of the specimen takes place within 2 minutes.

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