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1. 发明授权

US3902971A Biological detecting method and apparatus 失效
标题翻译：生物检测方法和装置
公开(公告)号：US3902971A
公开(公告)日：1975-09-02
申请号：US45952665
申请日：1965-05-20
申请人： AKZONA INC
发明人： FLETCHER JAMES C , HENDRIX CHARLES E , KILNER SCOTT B , LEHAN FRANK W , MAGILL PAUL L , MISHUCK ELI , PERKINS JR WILLIAM A , ROBINSON MERTON A , SWEENY KEITH H , TEW RICHARD W
IPC分类号： G01N33/569 , C12K1/04
CPC分类号： G01N33/569 , Y10S435/866 , Y10S435/871
摘要： This disclosure describes apparatus and the method for continuously sampling either gaseous or liquid media such as atmosphere or water to detect the presence or absence of specific pathogenic micro-organisms. The system as described employs collection apparatus which entrains particles of microorganism size in continuous flowing stream of water. A suspension of fluorescently tagged antibodies is injected into the flowing stream and thereby maintained in contact for a sufficient period of time for the specific antibodies to attach to any of its corresponding organism in the collection stream. The resultant mixture of the two streams is passed through a continuous filter having pore size selected to hold on its surface reacted antigenantibody complexes while passing the remainder of the affluent. After washing to remove extraneous residue, the surface of the filter is exposed to light of a wavelength which excites the fluorescently tagged antibodies if resident on the tape, and the number of such fluorescent particles appearing in the field of a scanned field is counted. In the absence of any fluorescent particles, the original collection stream is known to be free of the particular microorganism. The disclosure describes specific antibody-antigen complexes and their recommended fluorescent dye and exciting wavelength. A wide band preamplifier for detecting the signals on the system is similarly disclosed.
摘要翻译：本公开描述了用于连续取样气体或液体介质如大气或水的装置和方法，以检测特定致病微生物的存在或不存在。所描述的系统使用收集装置，其将连续流动的水中的微生物尺寸的颗粒夹带。将荧光标记的抗体的悬浮液注入流动流中，从而保持接触足够的时间以使特异性抗体附着于收集流中任何相应的生物体。将得到的两股物流混合物通过连续过滤器，该过滤器的孔径选择为保持其表面上反应的抗原 - 抗体复合物，同时通过富余物的其余部分。洗涤除去外来残留物后，将过滤器的表面暴露于激发荧光标记的抗体的波长的光，如果驻留在带上，并且计数在扫描场的场中出现的这种荧光颗粒的数量。在没有任何荧光颗粒的情况下，已知原始收集流不含特定微生物。本公开描述了特异性抗体 - 抗原复合物及其推荐的荧光染料和激发波长。类似地公开了用于检测系统上的信号的宽带前置放大器。

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