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71. 发明授权

US4822747A Polyacrylamide gel particles having hapten moieties bound thereto as immunoassay reagent 失效
标题翻译：具有作为免疫测定试剂结合的半抗原部分的聚丙烯酰胺凝胶颗粒
公开(公告)号：US4822747A
公开(公告)日：1989-04-18
申请号：US939902
申请日：1986-12-09
申请人： Richard D. Johnson , H. Volker Runzheimer , Ronald G. Sommer , Kin F. Yip
发明人： Richard D. Johnson , H. Volker Runzheimer , Ronald G. Sommer , Kin F. Yip
IPC分类号： G01N33/53 , G01N33/543 , G01N33/547 , G01N33/548 , G01N33/72 , G01N33/96
CPC分类号： G01N33/548 , G01N33/54313 , G01N33/723 , G01N33/96
摘要： An immobilized hapten reagent for use in a specific binding assay for the determination of a hapten or binding analog thereof in a liquid test sample and methods for preparing and using the immobilized hapten reagent. The immobilized hapten reagent comprises a hapten moiety covalently linked substantially only to the external surface of a polyacrylamide gel particle. The immobilized hapten reagent is prepared by forming a reaction mixture comprising the hapten moiety and the carrier material in a nonswelling solvent wherein the gel particle is substantially impervious to the hapten moiety and wherein an analytically insignificant amount of the hapten moiety is nonspecifically bound to the gel particle. The immobilized hapten reagent is characterized by being substantially stable in aqueous solutions and exhibits an insignificant amount of leakage of the hapten moiety into a liquid test medium.
摘要翻译：用于特异性结合测定中用于测定液体测试样品中的半抗原或结合类似物的固定化半抗原试剂以及制备和使用固定化半抗原试剂的方法。固定化的半抗原试剂包含基本上仅与聚丙烯酰胺凝胶颗粒的外表面共价连接的半抗原部分。通过在非溶剂溶剂中形成包含半抗原部分和载体材料的反应混合物来制备固定化的半抗原试剂，其中凝胶颗粒基本上不渗透半抗原部分，并且其中分析上不显着量的半抗原部分非特异性结合凝胶粒子。固定化的半抗原试剂的特征在于在水溶液中基本上是稳定的，并且将半抗原部分泄漏的液体试验介质的数量不大。

72. 发明授权

US4822565A Carrier device for immunological assay 失效
标题翻译：用于免疫测定的载体装置
公开(公告)号：US4822565A
公开(公告)日：1989-04-18
申请号：US710123
申请日：1985-03-11
申请人： Dora Kohler
发明人： Dora Kohler
IPC分类号： G01N33/544 , G01N33/548 , G01N35/10 , B01L9/00 , G01N33/543
CPC分类号： G01N33/548 , G01N33/544 , G01N35/1065 , Y10S436/809 , Y10S436/81
摘要： A carrier coated with antibodies or antigens is proposed, which performs a serological reaction with human, animal or plant material to be investigated and which is received in a multi-well plate. The carrier is made from nitrocellulose or a material having the adsorption characteristics of nitrocellulose and its shape is adapted to the multi-well plate used for performing serological reactions and is introduced into the latter. A plastic reinforcing structure provides support for the coated carrier. The coated carrier is not completely bound to the plastic reinforcing structure, so that both sides of the carrier will be contacted by material in the multi-well plate.
摘要翻译：提出了涂有抗体或抗原的载体，其与待研究的人，动物或植物材料进行血清学反应，并将其接收在多孔板中。载体由硝化纤维素或具有硝酸纤维素吸附特性的材料制成，其形状适用于用于进行血清学反应的多孔板，并引入其中。塑料增强结构为涂层载体提供支撑。涂覆的载体不完全结合到塑料增强结构，使得载体的两侧将与多孔板中的材料接触。

73. 发明授权

US4820627A Method of preparing particles suitable for tabletting into diagnostic reagents 失效
标题翻译：制备适于压片成诊断试剂的颗粒的方法
公开(公告)号：US4820627A
公开(公告)日：1989-04-11
申请号：US842968
申请日：1986-03-24
申请人： John K. McGeehan
发明人： John K. McGeehan
IPC分类号： C12Q1/00 , G01N33/48 , G01N33/531 , G01N33/548
CPC分类号： C12Q1/00 , G01N33/531 , G01N33/548 , Y10T436/10 , Y10T436/25
摘要： A completely water-soluble, solid, labile biochemical-containing diagnostic reagent is prepared without the need of a lyophilization step, by spraying, preferably by a fluidized bed process, an aqueous solution of labile biochemical, e.g., an enzyme, onto small particles, e.g., lower than 20 mesh, of an inert, completely water-soluble solid bulking agent, e.g., mannitol; drying the resultant labile biochemical-coated bulking agent to the desired dryness; and then forming resultant dried labile biochemical-coated bulking agent into a tablet suitable for a diagnostic test reagent, said tablet having a predetermined rate of dissolution.
摘要翻译：制备完全水溶性，固体，不稳定的含生物化学物质的诊断试剂，而不需要冷冻干燥步骤，优选通过流化床方法将不稳定生物化学物质例如酶的水溶液喷雾到小颗粒上，例如低于20目的惰性完全水溶性固体填充剂，例如甘露糖醇; 将所得不稳定的生物化学涂覆的填充剂干燥至所需的干燥度; 然后将得到的干燥不稳定的生物化学包衣的填充剂形成适用于诊断试剂的片剂，所述片剂具有预定的溶解速率。

74. 发明授权

US4778751A Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies 失效
标题翻译：使用配体标记的抗原或配体标记的抗体测量生物流体中的抗原或抗体的方法
公开(公告)号：US4778751A
公开(公告)日：1988-10-18
申请号：US862123
申请日：1986-05-12
申请人： A. Said El Shami , Olusola O. Alaba , Charles A. Kasal
发明人： A. Said El Shami , Olusola O. Alaba , Charles A. Kasal
IPC分类号： G01N33/545 , G01N33/531 , G01N33/538 , G01N33/543 , G01N33/548 , G01N33/537
CPC分类号： G01N33/54306 , G01N33/531 , G01N33/538 , G01N33/548 , Y10S435/805 , Y10S435/971 , Y10S436/823
摘要： The method of measuring circulating antigens or antibodies by using a ligand labeled specific antigen or ligand labeled specific antibody chemically attached to a soluble matrix or backbone, a differently labeled anti-antigen or anti-antibody and a solid phase anti-ligand directed at the ligand attached to the specific antigen or specific antibody. This is achieved by either one or two analytical schemes:(a) Reacting a patient sample with a ligand labeled specific antigen or a ligand labeled specific antibody in the liquid phase in the presence of a differently labeled specific anti-antigen or labeled specific anti-antibody. This immunological complex is reacted with an immobilized anti-ligand on a solid support which is directed against the ligand attached to the specific antigen or antibody through the liquid matrix. Subsequently the solid phase is washed and checked for the label on the anti-antigen or anti-antibody which is directly proportional to the concentration of specific antigen or antibody.(b) Reacting a patient sample with the ligand labeled specific antigen or a ligand labeled specific antibody in the liquid phase, then contacting said liquid phase with an immobilized anti-ligand on a solid support; washing the solid support and reacting it with a differently labeled anti-antigen or anti-antibody. This is followed by re-washing of the solid support and checking for the presence of the label attached to the anti-antigen or anti-antibody which is directly proportional to the concentration of the specific antigen or antibody. The ligand is covalently attached to the specific antigen or antibody through a soluble liquid matrix or backbone to which the specific antigen or antibody has been chemically attached. This invention is applicable to a wide variety of antigen or antibody detection as described herein.
摘要翻译：通过使用化学连接到可溶性基质或骨架的配体标记的特异性抗原或配体标记的特异性抗体，不同标记的抗原或抗体抗体和指向配体的固相抗配体来测量循环抗原或抗体的方法附着于特异性抗原或特异性抗体。这通过以下一种或两种分析方案实现：（a）在不同标记的特异性抗原或标记的特异性抗原的存在下，在液相中用配体标记的特异性抗原或配体标记的特异性抗体反应患者样品，抗体。该免疫复合物与固定支持物上的固定化抗配体反应，所述固定支持物通过液体基质针对与特定抗原或抗体连接的配体。随后洗涤固相并检查抗原或抗体上的标记，其与特异性抗原或抗体的浓度成正比。（b）在液相中用配体标记的特异抗原或配体标记的特异性抗体反应患者样品，然后将所述液相与固定支持物上的固定化抗配体接触; 洗涤固体支持物并使其与不同标记的抗原或抗体抗体反应。然后重新洗涤固体支持物并检查与抗原或抗体的附着物的存在，其与特异性抗原或抗体的浓度成正比。配体通过特定抗原或抗体化学连接的可溶性液体基质或骨架共价连接到特异性抗原或抗体上。本发明可应用于本文所述的多种抗原或抗体检测。

75. 发明授权

US4727037A Assay kit and method for the determination of antibody class and subclass 失效
标题翻译：用于测定抗体类和亚类的测定试剂盒和方法
公开(公告)号：US4727037A
公开(公告)日：1988-02-23
申请号：US580519
申请日：1984-02-15
申请人： David B. Ring
发明人： David B. Ring
IPC分类号： G01N33/543 , G01N33/548 , G01N33/68 , G01N33/53 , G01N33/577
CPC分类号： G01N33/548 , G01N33/543 , G01N33/6854 , Y10S435/805 , Y10S435/81 , Y10S435/97 , Y10S436/808 , Y10S436/809 , Y10S436/81
摘要： A method of rapid determination of the isotype class for a panel of monoclonal antibodies is described. The assay comprises adsorbing on a solid support medium antibodies directed to specific immunoglobulin heavy and light chains. Once such isotype-specific antibodies are bound to the nitrocellulose paper, the treated strips can be incubated with the monoclonal antibody of interest. Upon formation of a complex between the specific iso-type antisera and the monoclonal antibody, the complex is visualized by reaction with a chromogenic substance. In the preferred embodiment of the invention, the treated nitrocellulose strips are stored in kit form. Using these prepared strips, the isotyping assay can be performed in less than two hours with a minimum of technical manipulation and expenditure of reagents.
摘要翻译：描述了一组快速测定单克隆抗体单克隆抗体类别的方法。该测定包括吸附在固体支持培养基上针对特异性免疫球蛋白重链和轻链的抗体。一旦这样的同种型特异性抗体与硝化纤维素纸结合，则处理的条可以与感兴趣的单克隆抗体一起温育。在形成特异性异型抗血清和单克隆抗体之间的复合物时，通过与显色物质的反应可视化复合物。在本发明的优选实施方案中，经处理的硝酸纤维素条以试剂盒形式储存。使用这些制备的条带，可以在不到两个小时内进行同型化分析，同时以最小的技术操作和试剂消耗。

76. 发明授权

US4459360A Multiple-component binding assay system and method of making and using it 失效
标题翻译：多组分结合测定系统及其制备和使用方法
公开(公告)号：US4459360A
公开(公告)日：1984-07-10
申请号：US308935
申请日：1981-10-05
申请人： Vincent A. Marinkovich
发明人： Vincent A. Marinkovich
IPC分类号： C12M1/34 , A61K39/36 , B65D71/00 , C12Q1/00 , G01N33/52 , G01N33/53 , G01N33/543 , G01N33/548 , G01N33/58 , G01N33/60 , G01N23/06
CPC分类号： G01N33/521 , G01N33/548 , Y10S436/804 , Y10S436/808 , Y10S436/81
摘要： A multiple component binding assay system, and related methods of making and using it, having a plurality of coated filaments mounted at their opposite ends on a support, in a predetermined spaced relationship, for simultaneously screening a liquid test sample for a plurality of components. The system is particularly suitable for screening the sample for IgE class antibodies specific for certain allergens. The filaments are preferably cotton threads, each thread binding a different allergen through a covalent bond. The support, which is preferably cut away adjacent the mid-portion of each filament to reduce background interference, is composed of a plastic or other water insoluble material, permitting inexpensive and relatively simple manufacture. After any IgE class antibodies present in the liquid sample have reacted with the corresponding allergens coated on the filaments, they are detected using second antibodies labeled with radioactive, fluorescent or enzymatic tracers. By using a high energy radioactive tracer, such as .sup.125 I, autoradiographic techniques with phosphor screen enhancement allow for diagnostic analysis by a densitometer or even the unaided human eye.
摘要翻译：多组分结合测定系统及其制备和使用它的相关方法，其具有以预定的间隔关系安装在支撑体上的相对端的多个涂层细丝，用于同时筛选多个组分的液体测试样品。该系统特别适用于筛选样品中特定于某些过敏原的IgE类抗体。长丝优选为棉线，每根线通过共价键结合不同的变应原。优选地，邻近每根丝的中间部分切割以减少背景干扰的支撑体由塑料或其它水不溶性材料组成，允许廉价且相对简单的制造。在液体样品中存在的任何IgE类抗体已经与涂覆在细丝上的相应变应原反应后，使用用放射性，荧光或酶示踪剂标记的第二抗体检测它们。通过使用诸如125I的高能放射性示踪剂，具有荧光屏增强的放射自显影技术允许通过密度计或甚至无人检测的眼睛进行诊断分析。

77. 发明授权

US4330440A Activated matrix and method of activation 失效
标题翻译：活化基质和活化方法
公开(公告)号：US4330440A
公开(公告)日：1982-05-18
申请号：US128847
申请日：1980-03-10
申请人： John S. Ayers , Geoffrey S. Bethell , William S. Hancock , Milton T. W. Hearn
发明人： John S. Ayers , Geoffrey S. Bethell , William S. Hancock , Milton T. W. Hearn
IPC分类号： C08B15/06 , C08B37/00 , C12N11/10 , G01N33/543 , G01N33/548 , C08L1/00
CPC分类号： G01N33/548 , C08B15/06 , C08B37/0039 , C12N11/10 , G01N33/54353
摘要： The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affinity chromatography matrix which remains an uncharged species at varying pH's. The carbonylated product is also useful for the preparation of other compounds: Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose, typical macroporous synthetic polymers are acrylamides, acrylates and methacrylates, typical rigid supports are silica beads coated with hydroxy alkyl groups and typical cabonylating agents are N,N'-carbonyl diimidazole; N,N'carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.
摘要翻译：本发明涉及交联的取代和未取代的多糖，它们与大孔合成聚合物的共聚物，大孔合成聚合物和具有侧羟基烷基的刚性载体的羰基化。羰基化产物可用于制备在不同pH值下保持不带电荷的物质的亲和层析基质。羰基化产物也可用于制备其他化合物：典型的多糖是琼脂糖，淀粉，葡聚糖，纤维素和再生纤维素，典型的大孔合成聚合物是丙烯酰胺，丙烯酸酯和甲基丙烯酸酯，典型的刚性载体是用羟基烷基涂覆的二氧化硅珠，典型的卡罗胺化剂是N，N'-羰基二咪唑; N，N'-羰基二-1,2,3-苯并三唑; 和N，N'-羰基二-1,2,4-三唑。

78. 发明授权

US3847745A Preparation of water-insoluble biologically active materials 失效
标题翻译：水不溶性生物活性材料的制备
公开(公告)号：US3847745A
公开(公告)日：1974-11-12
申请号：US34318373
申请日：1973-03-20
申请人： EXPLOATERINGS AB TBF
发明人： AXEN R , VRETBLAD P , PORATH J
IPC分类号： A61K39/44 , A61K47/48 , C08B37/00 , C08F8/00 , C08G85/00 , C08H1/00 , C12N11/06 , C12N11/08 , C12N11/10 , C12Q1/00 , G01N33/543 , G01N33/548 , C07G7/02
CPC分类号： A61K39/44 , A61K47/58 , A61K47/61 , C08F8/00 , C12N11/08 , C12N11/10 , C12Q1/003 , G01N33/54353 , G01N33/548 , Y10S530/814 , Y10S530/816
摘要： Biologically active materials such as enzymes and hormones are coupled by an isonitrile group-containing compound to insoluble organic, hydrophilic polymeric carriers.
摘要翻译：诸如酶和激素的生物活性物质通过含异腈基的化合物偶联到不溶性有机的亲水性聚合物载体上。

79. 发明授权

US3694163A Test system for the determination of substances in test fluids and process for the preparation thereof 失效
标题翻译：用于测定液体中物质的测试系统及其制备方法
公开(公告)号：US3694163A
公开(公告)日：1972-09-26
申请号：US3694163D
申请日：1970-04-13
申请人： MILES LAB
发明人： SHERELIS ROBERT THOMAS
IPC分类号： C08L1/08 , C12Q1/00 , G01N13/00 , G01N27/40 , G01N33/487 , G01N33/548 , G01N35/00 , C12K1/10 , G01N31/06 , G01N33/16
CPC分类号： B01D69/141 , B01D71/16 , C12Q1/001 , G01N33/548 , Y10S435/805
摘要： A TEST SYSTEM AND DEVICE FOR THE DETERMINATION OF SUBSTANCES IN TEST FLUIDS WHICH UTILIZES A NOVEL CELLULOSE DERIVATIVE MEMBRANE IN CONJUNCTION WITH A TEST REAGENT SPECIFICALLY REACTABLE WITH THE SUBSTANCE BEING DETECTED AND SENSING MEANS FOR DETECTING ELECTRICAL, CHEMICAL OR PHYSICAL CHANGES IN THE MEMBRANE WHEN THE TEST REAGENT CONTACTS THE SUBSTANCE BEING DETECTED. THE MEMBRANE IS PREPARED USING A CONTROLLED ORGANIC PHASE EVAPORATION: AQUEOUS INVERSION PROCESS FROM A SOLVENT SOLUTION OF POLYMERIC CELLULOSE DERIVATIVE AND AN INTERACTING SWELLING AGENT, PREFERABLY FORMAMIDE, SAID REAGENT CONTAINED IN SAID SOLUTION AND THE RESULTING MEMBRANE IN EITHER DISSOLVED DISPERSED FORM.

80. 发明申请

US20180143191A1 Lateral flow membrane designed for multiparameter readouts and compact multiparameter lateral flow immunoassay device comprising the same 审中-公开
公开(公告)号：US20180143191A1
公开(公告)日：2018-05-24
申请号：US15315553
申请日：2015-04-28
申请人： Sartorius Stedim Biotech GmbH
发明人： Denise Van Rossum , Eric Jallérat , Susanne Drebing , Dieter Melzner
IPC分类号： G01N33/558 , G01N33/543 , G01N33/76 , G01N33/548 , B01L3/00 , G01N21/84
CPC分类号： G01N33/558 , B01L3/5023 , B01L3/502707 , B01L2200/12 , B01L2300/069 , B01L2300/0825 , B01L2300/0861 , B01L2300/0883 , B01L2400/0406 , G01N21/8483 , G01N33/50 , G01N33/538 , G01N33/54386 , G01N33/548 , G01N33/76 , G01N2333/59
摘要： The present invention relates to a multiparameter lateral flow strip (1), comprising a microporous membrane layer (2) supported on a liquid-impermeable support layer (3), for lateral flow of a liquid through the microporous membrane layer (2), wherein the microporous membrane layer (2) has two or more flow lanes (4) in the direction of lateral flow, wherein said two or more flow lanes (4) are separated by hydrophobic separation channels (5), and wherein each of said two or more flow lanes (4) comprises a detection spot (6) including a binding agent, wherein said two or more flow lanes (4) are isomorphic lanes. Furthermore, the present invention relates to a multiparameter lateral flow immunoassay device comprising said multiparameter lateral flow membrane (1), the use of said multiparameter configured lateral flow membrane (1) in an immunological test, as well as to a method for the manufacture of said multiparameter configured lateral flow membrane (1).

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