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1. 发明授权

US5470826A Single chain proteins with FHS suppressing activity isolated from follicular fluid and uses thereof 失效
标题翻译：从卵泡液分离出具有FHS抑制活性的单链蛋白及其用途
公开(公告)号：US5470826A
公开(公告)日：1995-11-28
申请号：US145194
申请日：1993-11-03
申请人： David M. De Kretser , Henry G. Burger , Milton T. W. Hearn , David M. Milne-Robertson , Richard E. H. Wettenhall , Robert I. McLachlan , Fiona De Vos
发明人： David M. De Kretser , Henry G. Burger , Milton T. W. Hearn , David M. Milne-Robertson , Richard E. H. Wettenhall , Robert I. McLachlan , Fiona De Vos
IPC分类号： A61K38/00 , A61K38/04 , A61K39/395 , A61P5/00 , A61P13/02 , A61P15/00 , C07K1/20 , C07K14/00 , C07K14/47 , C07K14/575 , C07K16/00 , C07K16/18 , C07K16/26 , C12P21/08 , A61K38/22
CPC分类号： C07K16/18 , C07K14/575 , C07K16/26 , A61K38/00 , Y10S530/85
摘要： Polypeptides exhibiting an inhibitory action over follitropin are disclosed. These polypeptides are designated follitropin suppressing proteins, or "FSP's," and range in size from 30 to 60 kD as determined by SDS-PAGE. Uses for FSP's, including regulation of fertility and as immunogens, are disclosed.
摘要翻译：本发明公开了对follitropin表现出抑制作用的多肽。这些多肽被命名为抑制蛋白质的蛋白质或“FSP”，并且通过SDS-PAGE测定，其尺寸范围为30至60kD。披露了FSP的用途，包括对生殖力的调节和作为免疫原。

2. 发明授权

US4224439A Activated matrix and method of activation 失效
标题翻译：活化基质和活化方法
公开(公告)号：US4224439A
公开(公告)日：1980-09-23
申请号：US874628
申请日：1978-02-02
申请人： John S. Ayers , Geoffrey S. Bethell , William S. Hancock , Milton T. W. Hearn
发明人： John S. Ayers , Geoffrey S. Bethell , William S. Hancock , Milton T. W. Hearn
IPC分类号： C08B15/06 , C08B37/00 , C12N11/10 , G01N33/543 , G01N33/548 , C08B3/00
CPC分类号： C12N11/10 , C08B15/06 , C08B37/0039 , G01N33/54353 , G01N33/548 , Y10S530/813
摘要： Carbonylation of a polysaccharide and the product of that carbonylation. The carbonylated product can be used to prepare an affinity chromatography matrix which is an uncharged species of varying pH's. The carbonylated product is also useful for the preparation of other compounds. Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose and typical carbonylating agents are N,N'-carbonyl diimidazole; N,N' carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.
摘要翻译：多糖的羰基化和该羰基化的产物。羰基化产物可用于制备不同pH值的不带电荷的亲和层析基质。羰基化产物也可用于制备其它化合物。典型的多糖是琼脂糖，淀粉，葡聚糖，纤维素和再生纤维素，典型的羰基化剂是N，N'-羰基二咪唑; N，N'羰基二-1,2,3-苯并三唑; 和N，N'-羰基二-1,2,4-三唑。

3. 发明授权

US4330440A Activated matrix and method of activation 失效
标题翻译：活化基质和活化方法
公开(公告)号：US4330440A
公开(公告)日：1982-05-18
申请号：US128847
申请日：1980-03-10
申请人： John S. Ayers , Geoffrey S. Bethell , William S. Hancock , Milton T. W. Hearn
发明人： John S. Ayers , Geoffrey S. Bethell , William S. Hancock , Milton T. W. Hearn
IPC分类号： C08B15/06 , C08B37/00 , C12N11/10 , G01N33/543 , G01N33/548 , C08L1/00
CPC分类号： G01N33/548 , C08B15/06 , C08B37/0039 , C12N11/10 , G01N33/54353
摘要： The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affinity chromatography matrix which remains an uncharged species at varying pH's. The carbonylated product is also useful for the preparation of other compounds: Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose, typical macroporous synthetic polymers are acrylamides, acrylates and methacrylates, typical rigid supports are silica beads coated with hydroxy alkyl groups and typical cabonylating agents are N,N'-carbonyl diimidazole; N,N'carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.
摘要翻译：本发明涉及交联的取代和未取代的多糖，它们与大孔合成聚合物的共聚物，大孔合成聚合物和具有侧羟基烷基的刚性载体的羰基化。羰基化产物可用于制备在不同pH值下保持不带电荷的物质的亲和层析基质。羰基化产物也可用于制备其他化合物：典型的多糖是琼脂糖，淀粉，葡聚糖，纤维素和再生纤维素，典型的大孔合成聚合物是丙烯酰胺，丙烯酸酯和甲基丙烯酸酯，典型的刚性载体是用羟基烷基涂覆的二氧化硅珠，典型的卡罗胺化剂是N，N'-羰基二咪唑; N，N'-羰基二-1,2,3-苯并三唑; 和N，N'-羰基二-1,2,4-三唑。

4. 发明授权

US4279724A Preparative electrofocusing in flat bed granulated polysaccharide gels 失效
标题翻译：在平板颗粒状多糖凝胶中制备电聚焦
公开(公告)号：US4279724A
公开(公告)日：1981-07-21
申请号：US58686
申请日：1979-07-18
申请人： Milton T. W. Hearn , Ross L. Prestidge
发明人： Milton T. W. Hearn , Ross L. Prestidge
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44795 , G01N27/44747
摘要： Methods are described for the generation of natural pH gradients in layers of granulated gels using mixtures of amphoteric or amphoteric non-amphoteric buffer reagents. Because of the favorable characteristics of these flat bed systems, high voltage gradients (20-50 V/cm) can be used for the electrofocusing experiments. Linear or step pH gradients can be formed depending on the choice of buffer system used. Buffer systems, suitable for the preparative electrofocusing of proteins over the pI ranges of 4.0-6.0, 4.5-8.0, 5.0-8.5 and 6.0-8.5 are described.
摘要翻译：描述了使用两性或两性非两性缓冲试剂的混合物在造粒凝胶层中产生天然pH梯度的方法。由于这些平床系统的有利特性，电聚焦实验可以使用高电压梯度（20-50V / cm）。根据所使用的缓冲系统的选择，可以形成线性或阶梯pH梯度。描述了适用于在4.0-6.0,4.5-8.0,5.0-8.5和6.0-8.5的pI范围内蛋白质的制备性电聚焦的缓冲体系。

5. 发明授权

US08748582B2 Affinity ligands and methods for protein purification 失效
标题翻译：亲和配体和蛋白质纯化方法
公开(公告)号：US08748582B2
公开(公告)日：2014-06-10
申请号：US13502103
申请日：2010-10-15
申请人： Milton T W Hearn , Anjali Makarand Bhagwat , William Roy Jackson , Simon John Mountford
发明人： Milton T W Hearn , Anjali Makarand Bhagwat , William Roy Jackson , Simon John Mountford
IPC分类号： C07K1/22 , C07D213/70 , C07D213/76 , C07D215/36 , C07D217/22 , C07D401/12 , C07D401/14 , C08B37/00
CPC分类号： B01D15/3804 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3244 , B01J20/3251 , B01J20/3255 , C07D213/32 , C07D213/70 , C07D215/36 , C07D217/22 , C07K1/22
摘要： The present invention relates generally to affinity ligands and chemical affinity ligand-matrix conjugates for use as chromatographic adsorbents and methods which utilize the adsorbents in the purification of proteins by affinity chromatography. The affinity ligand-matrix conjugates of the present invention comprise ligands of general formula (I): wherein m represents an integer from 0-2, n represents an integer from 0-6, p represents an integer from 0-4, R1 represents H or C1-3 alkyl, R2 is an optional substituent, and X is the position at which the ligand is immobilized, optionally via a linker.
摘要翻译：本发明一般涉及用作色谱吸附剂的亲和配体和化学亲和配体 - 基质共轭物以及利用吸附剂通过亲和层析纯化蛋白质的方法。本发明的亲和配体 - 基质缀合物包括通式（I）的配体：其中m表示0-2的整数，n表示0-6的整数，p表示0-4的整数，R1表示H 或C 1-3烷基，R 2是任选的取代基，并且X是任选地通过连接基固定配体的位置。

6. 发明授权

US06294174B1 Peptides immunologically related to known viral protein 失效
标题翻译：与已知病毒蛋白免疫相关的肽
公开(公告)号：US06294174B1
公开(公告)日：2001-09-25
申请号：US08290736
申请日：1994-11-16
申请人： Velibor Krsmanovic , Irena Cosic , Jean-Michel Biquard , Milton T. W. Hearn
发明人： Velibor Krsmanovic , Irena Cosic , Jean-Michel Biquard , Milton T. W. Hearn
IPC分类号： A61K3921
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , C07K1/00 , C07K14/001 , C07K14/70514 , C07K16/00 , C12N2740/16122 , C12N2740/16222
摘要： Peptides immunologically related to proteins expressed by a viral agent, having a sequence of amino acids ordered by means of the protein informational analysis techniques using the Fourier transform method with reference to the amino acid sequence of a target antigen against which antibodies are desired to be formed, or lymphocytes desired to be directed. The sequence has in the Fourier spectrum, one or more frequencies of practically the same value as the frequency of frequencies characteristic of the target antigen.
摘要翻译：与由病毒剂表达的蛋白免疫相关的肽，具有通过蛋白质信息分析技术使用傅里叶变换方法按照期望形成抗体的靶抗原的氨基酸序列排列的氨基酸序列进行免疫相关，或想要引导的淋巴细胞。该序列在傅里叶谱中具有与目标抗原特征频率的频率几乎相同的一个或多个频率。

7. 发明申请

US20120052757A1 MOLECULARLY IMPRINTED POLYMERS 审中-公开
标题翻译：分子印迹聚合物
公开(公告)号：US20120052757A1
公开(公告)日：2012-03-01
申请号：US13146970
申请日：2010-01-29
申请人： Milton T. W. Hearn , Steven Langford , Kellie Louise Tuck , Simon Harris , Reinhard Ingemar Boysen , Victoria Tamara Perchyonok , Basil Danylec , Lachlan Schwarz , Jamil Chowdhury
发明人： Milton T. W. Hearn , Steven Langford , Kellie Louise Tuck , Simon Harris , Reinhard Ingemar Boysen , Victoria Tamara Perchyonok , Basil Danylec , Lachlan Schwarz , Jamil Chowdhury
IPC分类号： C08J9/228 , C07C249/02 , C07C233/00 , C07C37/82 , C07F7/06 , B32B5/02 , C07C65/03 , C07C69/76 , C07C229/54 , C08F226/06 , C07J1/00
CPC分类号： B01J20/268 , A23L5/273 , A23V2002/00 , A61K31/74 , B01D15/3852 , B01J20/26 , B01J20/3057 , C07B63/00 , C07C37/82 , C08F226/06 , Y10T442/10
摘要： The present invention provides methods of designing molecularly imprinted polymers (MIPs) which have applications in extracting bioactive compounds from a range of bioprocessing feedstocks and wastes. The present invention is further directed to MIPs designed by the methods of the present invention.
摘要翻译：本发明提供了设计分子印迹聚合物（MIP）的方法，其可用于从一系列生物处理原料和废物中提取生物活性化合物。本发明还涉及通过本发明的方法设计的MIP。

8. 发明授权

US5102807A Inhibin isolated from ovarian follicular fluid 失效
标题翻译：从OVARIAN FOLLICULAR FLUID分离的盐酸盐
公开(公告)号：US5102807A
公开(公告)日：1992-04-07
申请号：US336052
申请日：1989-04-11
申请人： Henry G. Burger , David M. de Kretser , John K. Findlay , Francis J. Morgan , Milton T. W. Hearn , David Milne-Robertson , Robert G. Forage , Richard E. H. Weitenhall
发明人： Henry G. Burger , David M. de Kretser , John K. Findlay , Francis J. Morgan , Milton T. W. Hearn , David Milne-Robertson , Robert G. Forage , Richard E. H. Weitenhall
IPC分类号： C07K14/435 , A61K35/48 , A61K35/54 , A61K38/00 , A61K38/22 , A61K39/00 , A61K39/395 , C07K1/16 , C07K1/20 , C07K1/26 , C07K14/575 , C07K16/00 , C07K16/26 , G01N33/68
CPC分类号： G01N33/689 , C07K14/575 , C07K16/26 , A61K38/00 , A61K39/00 , Y10S436/817 , Y10S530/806 , Y10S530/85
摘要： A protein which satisfies all the biological criteria which are characteristic of inhibin has been isolated from a gonadal source. The purification and characterization of inhibin and the use of the purified material to raise antibodies, the use of inhibin and said antisera in a quantative radioimmunoassay, and applications in vitro and in vivo of inhibin and antibody to inhibin, are described.There is provided a purified protein, inhibin, characterised in thata. the apparent molecular weight as determined by SDS-PAGE is 56,000.+-.1,000b. the isoelectric point is in the range 6.9-7.3c. the protein can bind specifically to Concanavalin A-Sepharosed the protein consists of two sub-units, characterized in thati. their apparent molecular weights as determined by SDS-PAGE are 44,000.+-.3,000 and 14,000.+-.2,000 respectively.ii. the isoelectric point of the 44,000 molecular weight sub-unit is in the range 6.0-7.0iii. the N-terminal amino acid sequences of the two sub-units are as described hereine. the protein can suppress follicle stimulating hormone (FSH) but not luteinising hormone (LH), thyroid stimulating hormone or prolactin in an in vitro bioassay systemf. the protein can be labeled with radioactive iodine.There is also provided a method for isolating and purifying inhibin from mammalian ovarian follicular fluid, characterised bya. one or more gel permeation chromatography steps;b. one or more reversed-phase high performance liquid chromatography steps;c. one or more preparative polyacrylamide gel electrophoresis steps;d. Electrophoretic elution of the purified inhibin.
摘要翻译：已经从性腺源分离了满足抑制素特征的所有生物学标准的蛋白质。纯化和抑制素的表征和使用该纯化的物质的产生抗体，使用抑制素和所述一个定量放射免疫测定的抗血清，并在体外和抑制素和抗体抑制素的体内应用，进行了描述。提供了一种纯化的蛋白质抑制素，其特征在于a。通过SDS-PAGE测定的表观分子量为56,000 +/- 1,000b。等电点在6.9-7.3 c的范围内。蛋白质可以特异性结合伴刀豆球蛋白A-Sepharose d，蛋白质由两个亚单位组成，其特征在于i。它们通过SDS-PAGE测定的表观分子量分别为44,000 +/- 3,000和14,000 +/- 2,000。 ii。 44,000分子量子单元的等电点在6.0-7.0 iii范围内。两个亚单位的N-末端氨基酸序列如本文所述。该蛋白质能够抑制促卵泡激素（FSH），但不黄体生成激素（LH），促甲状腺激素或促乳素在体外生物测定系统F。蛋白质可以用放射性碘标记。还提供了一种从哺乳动物卵巢滤泡液中分离和纯化抑制素的方法，其特征在于a。一个或多个凝胶渗透色谱步骤; b。一个或多个反相高效液相色谱步骤; C。一个或多个制备型聚丙烯酰胺凝胶电泳步骤; d。纯化的抑制素的电泳洗脱。

9. 发明授权

US5364837A Method of modifying serum hormone levels using purified inhibin materials 失效
标题翻译：使用纯化的抑制素材料修饰血清激素水平的方法
公开(公告)号：US5364837A
公开(公告)日：1994-11-15
申请号：US389328
申请日：1989-08-03
申请人： Henry G. Burger , David M. de Kretser , John K. Findlay , Francis J. Morgan , Milton T. W. Hearn , David M. Milne-Robertson , Robert G. Forage
发明人： Henry G. Burger , David M. de Kretser , John K. Findlay , Francis J. Morgan , Milton T. W. Hearn , David M. Milne-Robertson , Robert G. Forage
IPC分类号： C07K14/435 , A61K35/48 , A61K35/54 , A61K38/00 , A61K38/22 , A61K39/00 , A61K39/395 , C07K1/16 , C07K1/20 , C07K1/26 , C07K14/575 , C07K16/00 , C07K16/26 , G01N33/68 , A61K37/24
CPC分类号： G01N33/689 , C07K14/575 , C07K16/26 , A61K38/00 , A61K39/00 , Y10S436/817 , Y10S530/806 , Y10S530/85
摘要： A protein which satisfies all the biological criteria which are characteristic of inhibin has been isolated from a gonadal source. The purification and characterization of inhibin and the use of the purified material to raise antibodies, the use of inhibin and said antisera in a quantative radioimmunoassay, and application in vitro and in vivo of inhibin and antibody to inhibin, are described. There is provided a purified protein, inhibin characterized in that the apparent molecular weight as determined by SDS-PAGE is 56,000.+-.1,000, the isoelectric point is in the range 6.9-7.3, and the protein can bind specifically to Concanavalin A-Sepharose. Moreover, the protein includes two subunits, characterized in that their apparent molecular weights as determined by SDS-PAGE are 44,000.+-.3,000 and 14,000.+-.2,000, respectively. Furthermore, the isoelectric point of the 44,000 molecular weight sub-unit is in the range of 6.0-7.0. In addition, the N-terminal amino acid sequence of the two subunits are described. With regard to the purified protein, inhibin, the protein can suppress follicle stimulating hormone (FSH) but not luteinizing hormone (EACH), thyroid stimulating hormone or prolactin in an in vitro bioassay system, and the protein can be labeled with radioactive iodine. In addition to the forgoing, there is also provided a method for isolating and purifying inhibin from mammalian ovarian follicular fluid, characterized by one or more gel permeation chromatography steps; reversed-phase high performance liquid chromatography steps; preparative polyacrylamide gel electrophoresis steps; and electrophoretic elution of the purified inhibin.
摘要翻译：已经从性腺源分离了满足抑制素特征的所有生物学标准的蛋白质。描述了抑制素的纯化和表征以及纯化材料用于产生抗体的用途，在定量放射免疫测定中使用抑制素和所述抗血清，以及体外和体内抑制素和抗体对抑制素的应用。提供了纯化的蛋白质，抑制素，其特征在于通过SDS-PAGE测定的表观分子量为56,000 +/- 1,000，等电点在6.9-7.3范围内，蛋白质可以特异性结合伴刀豆球蛋白A-琼脂糖凝胶。此外，蛋白质包括两个亚基，其特征在于，其通过SDS-PAGE测定的表观分子量分别为44,000 +/- 3,000和14,000 +/- 2,000。此外，44,000分子量子单元的等电点在6.0-7.0的范围内。此外，描述了两个亚基的N-末端氨基酸序列。关于纯化的蛋白质，抑制素，蛋白质可以在体外生物测定系统中抑制卵泡刺激素（FSH），而不是黄体生成激素（EACH），促甲状腺激素或催乳素，并且蛋白质可以用放射性碘标记。除了上述之外，还提供了分离和纯化哺乳动物卵泡滤液中抑制素的方法，其特征在于一个或多个凝胶渗透色谱步骤; 反相高效液相色谱步骤; 制备聚丙烯酰胺凝胶电泳步骤; 和纯化的抑制素的电泳洗脱。

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