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1. 发明授权

US4595524A Two component stain composition for producing a Giemsa blood stain effect 失效
标题翻译：用于产生吉姆萨血液染色效果的双组分染色组合物
公开(公告)号：US4595524A
公开(公告)日：1986-06-17
申请号：US594182
申请日：1984-03-28
申请人： Kin F. Yip , Frances F. Rhodes
发明人： Kin F. Yip , Frances F. Rhodes
IPC分类号： G01N1/30 , G01N33/50 , G01N31/00 , B66D3/04 , G01N1/00
CPC分类号： G01N33/5094 , G01N2001/305 , Y10T436/10 , Y10T436/107497 , Y10T436/108331
摘要： Disclosed is a novel, 2 component Giemsa stain. The stain comprises a first component which is an aqueous solution of methylene blue, Azure B and a preservative and a second component which is an acetone/dimethyl formamide/water solvent system containing the sodium salt of eosin Y and a buffer. The Giemsa stain effect is obtained by first fixing a blood slide with methanol and then applying the freshly mixed first and second components of the stain. The slide is then rinsed and dried to prepare it for examination.
摘要翻译：公开了一种新颖的2组分吉姆萨染色。染色剂包括作为亚甲基蓝的水溶液的第一组分，Azure B和防腐剂，第二组分是含有曙红Y的钠盐和缓冲剂的丙酮/二甲基甲酰胺/水溶剂体系。通过首先用甲醇固定血液载玻片，然后施加新鲜混合的染色剂的第一和第二成分，获得吉姆萨染色效果。然后将载玻片漂洗并干燥以准备检查。

2. 发明授权

US5385847A Method for the determination of urinary protein and creatinine 失效
标题翻译：测定尿蛋白和肌酐的方法
公开(公告)号：US5385847A
公开(公告)日：1995-01-31
申请号：US160239
申请日：1993-12-02
申请人： Kin F. Yip , Amy H. Chu , Brenda Tudor
发明人： Kin F. Yip , Amy H. Chu , Brenda Tudor
IPC分类号： B01L3/00 , G01N33/493 , G01N33/53 , G01N33/68 , G01N33/70 , G01N33/546 , G01N33/00 , G01N33/557
CPC分类号： B01L3/502 , G01N33/493 , G01N33/6827 , G01N33/70 , Y10S435/973 , Y10T436/147777
摘要： Disclosed is a method for the determination of urinary protein and creatinine in a single reaction vessel using a continuous process. The method involves adjusting the pH to a level suitable for carrying out an immuno assay for the protein and making such a determination by an immuno agglutination technique. Raising the pH to a level suitable for determining the creatinine quickly dissipates the cloudiness caused by the agglutination reaction, so that the creatinine determination can be carried out without delay.
摘要翻译：公开了一种使用连续方法在单个反应容器中测定尿蛋白和肌酐的方法。该方法包括将pH调节到适于进行蛋白质免疫测定的水平，并通过免疫凝集技术进行这种测定。将pH提高到适合于测定肌酸酐的水平，迅速消散由凝集反应引起的混浊，从而可以毫不拖延地进行肌酸酐测定。

3. 发明授权

US4968742A Preparation of ligand-polymer conjugate having a controlled number of introduced ligands 失效
标题翻译：具有受控数目的引入配体的配体 - 聚合物缀合物的制备
公开(公告)号：US4968742A
公开(公告)日：1990-11-06
申请号：US118566
申请日：1987-11-09
申请人： Lynette A. Lewis , Kin F. Yip
发明人： Lynette A. Lewis , Kin F. Yip
IPC分类号： C08F8/00 , G01N33/531 , G01N33/543 , G01N33/545 , G01N33/547 , G01N33/72
CPC分类号： G01N33/545 , G01N33/531 , G01N33/54313 , G01N33/723
摘要： A method for chemically coupling a controllable number of ligands, e.g., haptens, to a polymeric material by derivatization of the polymer with an activating agent to introduce a couplable functional group. The derivatization is performed in the presence of a blocking agent which is reactive with the same functionality on the polymer as the activating agent. By reacting the polymer with a mixture of a predetermined ratio of excess amounts of the activating and blocking agents, a controllable number of couplable functional groups are introduced to the polymer for subsequent linkage to the desired ligand. The resulting polymer-ligand conjugates are useful as reagents in immunoassays, particularly immunoturbidimetric assays.

4. 发明授权

US4822747A Polyacrylamide gel particles having hapten moieties bound thereto as immunoassay reagent 失效
标题翻译：具有作为免疫测定试剂结合的半抗原部分的聚丙烯酰胺凝胶颗粒
公开(公告)号：US4822747A
公开(公告)日：1989-04-18
申请号：US939902
申请日：1986-12-09
申请人： Richard D. Johnson , H. Volker Runzheimer , Ronald G. Sommer , Kin F. Yip
发明人： Richard D. Johnson , H. Volker Runzheimer , Ronald G. Sommer , Kin F. Yip
IPC分类号： G01N33/53 , G01N33/543 , G01N33/547 , G01N33/548 , G01N33/72 , G01N33/96
CPC分类号： G01N33/548 , G01N33/54313 , G01N33/723 , G01N33/96
摘要： An immobilized hapten reagent for use in a specific binding assay for the determination of a hapten or binding analog thereof in a liquid test sample and methods for preparing and using the immobilized hapten reagent. The immobilized hapten reagent comprises a hapten moiety covalently linked substantially only to the external surface of a polyacrylamide gel particle. The immobilized hapten reagent is prepared by forming a reaction mixture comprising the hapten moiety and the carrier material in a nonswelling solvent wherein the gel particle is substantially impervious to the hapten moiety and wherein an analytically insignificant amount of the hapten moiety is nonspecifically bound to the gel particle. The immobilized hapten reagent is characterized by being substantially stable in aqueous solutions and exhibits an insignificant amount of leakage of the hapten moiety into a liquid test medium.
摘要翻译：用于特异性结合测定中用于测定液体测试样品中的半抗原或结合类似物的固定化半抗原试剂以及制备和使用固定化半抗原试剂的方法。固定化的半抗原试剂包含基本上仅与聚丙烯酰胺凝胶颗粒的外表面共价连接的半抗原部分。通过在非溶剂溶剂中形成包含半抗原部分和载体材料的反应混合物来制备固定化的半抗原试剂，其中凝胶颗粒基本上不渗透半抗原部分，并且其中分析上不显着量的半抗原部分非特异性结合凝胶粒子。固定化的半抗原试剂的特征在于在水溶液中基本上是稳定的，并且将半抗原部分泄漏的液体试验介质的数量不大。

5. 发明授权

US4774340A Method for preparing 3-hydroxy pyrroles and esters thereof 失效
标题翻译： 3-羟基吡咯及其酯的制备方法
公开(公告)号：US4774340A
公开(公告)日：1988-09-27
申请号：US855735
申请日：1986-04-25
申请人： Paul F. Corey , Frederick E. Ward , Kin F. Yip , Meitak T. Yip
发明人： Paul F. Corey , Frederick E. Ward , Kin F. Yip , Meitak T. Yip
IPC分类号： C07D207/36 , C07D303/48 , C07D333/32 , C07D339/04 , C12Q1/37 , C12Q1/44
CPC分类号： C07D333/32 , C07D207/36 , C07D303/48 , C07D339/04 , C12Q1/37 , C12Q1/44
摘要： A novel compound useful in detecting leukocytes, esterase and protease in a test sample. The compound has the structure ##STR1## in which: A is an acid residue, R is lower alkyl, aryl, carboxyl, carboxyl ester, amido or cyano, R* is H or lower alkyl, and X is O, S, or NR', in which R' is H, lower alkyl or aryl.
摘要翻译：用于检测试样中白细胞，酯酶和蛋白酶的新型化合物。该化合物具有以下结构：其中：A为酸残基，R为低级烷基，芳基，羧基，羧基酯，酰氨基或氰基，R *为H或低级烷基，X为O，S或NR '，其中R'是H，低级烷基或芳基。

6. 发明授权

US5045480A Gel particles having hapten moieties bound thereto as immunoassay reagent 失效
标题翻译：具有作为免疫测定试剂结合的半抗原部分的凝胶颗粒
公开(公告)号：US5045480A
公开(公告)日：1991-09-03
申请号：US308658
申请日：1989-02-09
申请人： Richard D. Johnson , H.-Volker Runzheimer , Ronald G. Sommer , Kin F. Yip
发明人： Richard D. Johnson , H.-Volker Runzheimer , Ronald G. Sommer , Kin F. Yip
IPC分类号： G01N33/543
CPC分类号： G01N33/54353 , G01N33/54313
摘要： An immobilized hapten reagent for use in a specific binding assay for the determination of a hapten or binding analog thereof in a liquid test sample and methods for preparing and using the immobilized hapten reagent. The immobilized hapten reagent comprises a hapten moiety covalenty linked substantially only to the external surface of a gel particle. The immobilized hapten reagent is prepared by forming a reaction mixture comprising the hapten moiety and the carrier material in a nonswelling solvent wherein the gel particle is substantially impervious to the hapten moiety and wherein an analytically insignificant amount of the hapten moiety is nonspecifically bound to the gel particle. The immobilized hapten reagent is characterized by being substantially stable in aqueous solutions and exhibits an insignificant amount of leakage of the hapten moiety into a liquid test medium.
摘要翻译：用于特异性结合测定中用于测定液体测试样品中的半抗原或结合类似物的固定化半抗原试剂以及制备和使用固定化半抗原试剂的方法。固定化的半抗原试剂包含基本上仅与凝胶颗粒的外表面共价连接的半抗原部分。通过在非溶剂溶剂中形成包含半抗原部分和载体材料的反应混合物来制备固定化的半抗原试剂，其中凝胶颗粒基本上不渗透半抗原部分，并且其中分析上不显着量的半抗原部分非特异性结合凝胶粒子。固定化的半抗原试剂的特征在于在水溶液中基本上是稳定的，并且将半抗原部分泄漏的液体试验介质的数量不大。

7. 发明授权

US4552697A Compound useful in detecting ions and method of preparing it 失效
标题翻译：用于检测离子的化合物及其制备方法
公开(公告)号：US4552697A
公开(公告)日：1985-11-12
申请号：US493951
申请日：1983-05-12
申请人： Kin F. Yip , Steven C. Charlton
发明人： Kin F. Yip , Steven C. Charlton
IPC分类号： C07C49/67 , C07C49/747 , C07C51/347 , C07C57/30 , C07C59/84 , C07C67/00 , C07C241/00 , C07C249/02 , C07C251/20 , C07C251/22 , G01N33/84 , C07C97/22
CPC分类号： G01N33/84 , C07C49/67 , C07C49/747 , C07C51/347 , C07C57/30 , C07C59/84
摘要： A novel compound is disclosed having the structure ##STR1## in which R is H or lower alkyl, R* is intermediate alkyl, and X is halogen or pseudohalogen. Also disclosed is a method for preparing the compound, the method including the steps of reacting (intermediate alkyl)benzene (I) with succinic anhydride to form (intermediate alkyl)-benzoylpropionic acid (II), successively reducing and dehydrating (II) to form 7-(intermediate alkyl)-1-tetralone (III), alkylating (III) to form 2-hydroxy(lower alkylidene)-7-(intermediate alkyl)-1-tetralone (IV), acrylating (IV) to form 2-acyloxy(lower alkylidene)-7-(intermediate alkyl)-1-tetralone (V), reacting (V) in the presence of an cycloalkene to form 7(intermediate alkyl)-2-(lower alkyl)-1-naphthol (VI), reacting (VI) with a 2,6-dihaloquinone-4-haloimide to form the compound (I), and isolating it.
摘要翻译：公开了具有结构式（Ⅰ）的新化合物，其中R为H或低级烷基，R *为中间体烷基，X为卤素或假卤素。还公开了一种制备该化合物的方法，该方法包括使（中间体烷基）苯（I）与琥珀酸酐反应形成（中间体烷基） - 苯甲酰基丙酸（II）的步骤，依次还原和脱水（II）形成 7-（中间体烷基）-1-四氢萘酮（III），烷基化（III）以形成2-羟基（低级亚烷基）-7-（中间体烷基）-1-四氢萘酮（IV），丙烯酸酯化（IV）酰基氧基（低级亚烷基）-7-（中间体烷基）-1-四氢萘酮（V），使（V）在环烯烃存在下反应形成7（中间体烷基）-2-（低级烷基）-1-萘酚），使（VI）与2,6-二卤代醌-4-卤代酰亚胺反应以形成化合物（I），并将其分离。

8. 发明授权

US4392864A Stabilized Romanowsky stain solution 失效
标题翻译：稳定的Romanowsky染色溶液
公开(公告)号：US4392864A
公开(公告)日：1983-07-12
申请号：US344390
申请日：1982-02-01
申请人： Elaine A. Helfrich , Kin F. Yip
发明人： Elaine A. Helfrich , Kin F. Yip
IPC分类号： C09B67/10 , C09B67/22 , C09B67/44 , G01N1/30 , G01N33/48 , C09B44/00 , G01N1/00
CPC分类号： G01N1/30 , C09B67/0033 , C09B67/0083
摘要： Disclosed is a Romanowsky stain having improved stability characteristics which comprises a methanol solution of Methylene Blue, an azure dye and the free acid of Eosin Y.
摘要翻译：公开了具有改进的稳定性特征的Romanowsky染色剂，其包括亚甲蓝的甲醇溶液，天青染料和曙红Y的游离酸。

9. 发明授权

US5620579A Apparatus for reduction of bias in amperometric sensors 失效
标题翻译：用于减小电流传感器偏压的装置
公开(公告)号：US5620579A
公开(公告)日：1997-04-15
申请号：US435993
申请日：1995-05-05
申请人： Marvin A. Genshaw , Dijia Huang , Matthew K. Musho , Kin F. Yip
发明人： Marvin A. Genshaw , Dijia Huang , Matthew K. Musho , Kin F. Yip
IPC分类号： G01N33/483 , C12Q1/00 , G01N27/327 , G01N27/416 , G01N27/48 , G01N33/66 , G01N33/72 , G01N33/92 , G01N27/26
CPC分类号： C12Q1/004
摘要： Apparatus and method are provided for determining the concentration of an analyte in a fluid test sample by applying the fluid test sample to the surface of a working electrode which is electrochemically connected to a reference electrode which surface bears a composition comprising an enzyme specific for the analyte. A mediator is reduced in response to a reaction between the analyte and the enzyme. An oxidizing potential is applied between the electrodes to return at least a portion of the mediator back to its oxidized form before determining the concentration of the analyte to thereby increase the accuracy of the analyte determination. Following this initially applied potential, the circuit is switched to an open circuit or to a potential that substantially reduces the current to minimize the rate of electrochemical potential at the working electrode. A second potential is applied between the electrodes and the current generated in the fluid test sample is measured to determine analyte concentration. Optionally, the accuracy of the analyte determination is further enhanced algorithmically.
摘要翻译：提供了设备和方法，用于通过将流体测试样品施加到电化学连接到参考电极的工作电极的表面上来确定流体测试样品中分析物的浓度，所述参考电极的表面承载包含对于分析物特异的酶的组合物。响应分析物和酶之间的反应而减少介体。在确定分析物的浓度之前，在电极之间施加氧化电位以将至少一部分介体返回到其氧化形式，从而提高分析物确定的准确度。在该最初施加的电位之后，电路被切换到开路或电位，其基本上减小电流以最小化工作电极处的电化学电势的速率。在电极之间施加第二电位，并测量在流体测试样品中产生的电流以确定分析物浓度。任选地，分析物确定的准确度进一步在算法上增强。

10. 发明授权

US5372948A Assay and reaction vessel with a compartmentalized solubilization chamber 失效
标题翻译：测定和反应容器与分隔的溶解室
公开(公告)号：US5372948A
公开(公告)日：1994-12-13
申请号：US32643
申请日：1993-03-17
申请人： Kin F. Yip
发明人： Kin F. Yip
IPC分类号： G01N31/22 , B01L3/00 , G01N33/48 , G01N33/53 , G01N33/543 , G01N33/72 , G01N33/539 , G01N33/546
CPC分类号： B01L3/502 , G01N33/723 , G01N33/726
摘要： Disclosed are a method and device for performing sequential analytical reactions involving a first dry reagent and a second dry reagent comprised of two or more components having different rates of solubilization. The invention enables one to fully solubilize the components of the second reagent before they are brought into contact with each other to thereby avoid interference with the reaction kinetics which result when one or both of the components are not fully dissolved prior to their being brought into contact. The invention is especially useful in conjunction with immunoassay formats involving latex bound antibodies and polymeric agglutinators.
摘要翻译：公开了一种用于执行包括第一干试剂和由两种或更多种具有不同溶解速率的组分组成的第二干试剂的顺序分析反应的方法和装置。本发明使得能够使第二试剂的组分在它们彼此接触之前完全溶解，从而避免干扰当其中一种或两种组分在其被接触之前不完全溶解时产生的反应动力学。本发明特别适用于涉及胶乳结合抗体和聚合凝集素的免疫测定方法。

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