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51. 发明申请

US20140363852A1 METHODS AND APPARATUS FOR SYNTHESIZING NUCLEIC ACIDS 有权
标题翻译：用于合成核酸的方法和装置
公开(公告)号：US20140363852A1
公开(公告)日：2014-12-11
申请号：US14459014
申请日：2014-08-13
申请人： Molecular Assemblies, Inc.
发明人： J. William Efcavitch , Suhaib Siddiqi
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , B01J2219/00596 , B01J2219/00722 , C12P19/34 , C12Q1/06 , C12Q1/6806 , C12Q2521/101 , C12Q2525/117 , Y02P20/582
摘要： The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template. Because the nucleotide analogs have an unmodified 3′ OH, i.e., as found in “natural” deoxyribose and ribose molecules, the analogs result in natural polynucleotides suitable for incorporation into biological systems.
摘要翻译：本发明提供使用酶和特别设计的核苷酸类似物合成多核苷酸（例如DNA和RNA）的改进方法。使用本发明的方法，多核苷酸的特异性序列可以在碱性水溶液环境中从头合成，而不使用核酸模板。因为核苷酸类似物具有未修饰的3'OH，即如在“天然”脱氧核糖和核糖分子中所发现的那样，所述类似物产生适合并入生物系统的天然多核苷酸。

52. 发明授权

US08859205B2 Methods and compositions for improving efficiency of nucleic acids amplification reactions 有权
标题翻译：提高核酸扩增反应效率的方法和组合物
公开(公告)号：US08859205B2
公开(公告)日：2014-10-14
申请号：US12683950
申请日：2010-01-07
申请人： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
发明人： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
IPC分类号： C12Q1/68 , C12P19/34 , C07K1/00 , C12N9/12 , C07K16/40
CPC分类号： C12P19/34 , C07K16/40 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2319/80 , C12N9/1252 , C12Q1/6804 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2521/101
摘要： The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
摘要翻译：本发明提供了提高核酸扩增反应效率的方法和组合物。本发明包括显示比野生型聚合酶增加的活性以及降低的外切核酸酶活性的杂合聚合酶。本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。

53. 发明授权

US08741617B2 DNA polymerases with increased 3′-mismatch discrimination 有权
公开(公告)号：US08741617B2
公开(公告)日：2014-06-03
申请号：US13162689
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
IPC分类号： C12N9/00 , C12N15/54
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6827 , C12Q1/6848 , C12Q1/6858 , C12Q1/686 , C12Y207/07007 , C12Q2521/101 , C12Q2535/125
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

54. 发明授权

US08722583B2 Method for selecting a chemical entity from a tagged library 有权
标题翻译：从标记库选择化学实体的方法
公开(公告)号：US08722583B2
公开(公告)日：2014-05-13
申请号：US11402957
申请日：2006-04-13
申请人： Alex Haahr Gouliaev , Thomas Franch
发明人： Alex Haahr Gouliaev , Thomas Franch
IPC分类号： C40B20/04 , C12Q1/68 , C07H21/04
CPC分类号： C12N15/1065 , C12N15/1068 , C12Q1/6806 , C12Q2521/101 , C12Q2521/501 , C40B50/10 , C40B50/16
摘要： Disclosed is a method for determining the identity of a chemical entity having a preselected property. The chemical entity is identified from a library composed of a plurality of different chemical entities each appended to unique identifier tags. An anti-tag having the capability of specifically interacting with the unique identifier tag is recovered during the method and used for identification purposes.
摘要翻译：公开了一种用于确定具有预选性质的化学实体的身份的方法。从由多个不同化学实体组成的库中识别化学实体，每个化学实体各自附加到唯一标识符标签。在该方法期间恢复具有与唯一标识符标签特异性交互的能力的反标签，并用于识别目的。

55. 发明申请

US20140113349A1 Solubilized Phospholipids for Stabilizing Nucleic Acid Polymerases 有权
标题翻译：用于稳定核酸聚合酶的溶解磷脂
公开(公告)号：US20140113349A1
公开(公告)日：2014-04-24
申请号：US14136152
申请日：2013-12-20
申请人： New England Biolabs, Inc.
发明人： Donald Johnson , Thomas C. Evans, JR.
IPC分类号： C12N9/96
CPC分类号： C12N9/96 , C12N9/1241 , C12Q1/686 , C12Q2521/101 , C12Q2527/127
摘要： Compositions and methods are provided that relate to solubilized phospholipids and their use in stabilizing nucleic acid polymerases. For example, a phospholipid with a tail containing at least 8 carbons can be solubilized in the presence of an amphipathic molecule.
摘要翻译：提供了涉及溶解磷脂及其在稳定核酸聚合酶中的用途的组合物和方法。例如，具有至少8个碳的尾部的磷脂可以在两亲性分子的存在下溶解。

56. 发明授权

US08691968B2 Compositions and methods for the protection of nucleophilic groups 有权
标题翻译：用于保护亲核基团的组合物和方法
公开(公告)号：US08691968B2
公开(公告)日：2014-04-08
申请号：US13001735
申请日：2009-07-02
申请人： Fei Mao , Xing Xin , Wai-Yee Leung
发明人： Fei Mao , Xing Xin , Wai-Yee Leung
IPC分类号： C07H21/00 , C12Q1/68 , B01L3/00
CPC分类号： C12Q1/6844 , C07K1/064 , C08H1/00 , C12N9/1252 , C12N9/127 , C12P19/34 , C12Q2521/101 , C12Q2521/119
摘要： The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention.
摘要翻译：本发明提供了与包含亲核基团的分子的保护和去保护有关的组合物，方法和试剂盒，例如耐热聚合酶的保护和去保护。还提供使用根据本发明保护的聚合酶进行核酸扩增的方法。

57. 发明授权

US08685675B2 Enzymes for amplification and copying bisulphite modified nucleic acids 有权
标题翻译：用于扩增和复制亚硫酸氢盐修饰的核酸的酶
公开(公告)号：US08685675B2
公开(公告)日：2014-04-01
申请号：US12744305
申请日：2008-11-27
申请人： Douglas Spencer Millar
发明人： Douglas Spencer Millar
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12N9/1252 , C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/101
摘要： The invention relates to the use of enzymes for copying or amplifying bisulphite modified or treated nucleic acids, wherein the enzymes are more effective in copying or amplifying the nucleic acid compared with native Taq polymerase under substantially the same conditions.
摘要翻译：本发明涉及用于复制或扩增亚硫酸氢盐修饰或处理的核酸的酶的用途，其中所述酶在基本相同的条件下与天然Taq聚合酶相比更有效地复制或扩增核酸。

58. 发明授权

US08614072B2 Polymerase incorporation of non-standard nucleotides 有权
标题翻译：聚合酶掺入非标准核苷酸
公开(公告)号：US08614072B2
公开(公告)日：2013-12-24
申请号：US12999138
申请日：2009-06-16
申请人： Steven Albert Benner , Zunyi Yang
发明人： Steven Albert Benner , Zunyi Yang
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6853 , C12P19/34 , C12Q1/686 , C12Q2521/101 , C12Q2525/117 , C12Q2525/191
摘要： The disclosed invention teaches processes to amplify oligonucleotides by contacting templates and primers with DNA polymerases and triphosphates of non-standard nucleotides, which form nucleobase pairs fitting the standard Watson-Crick geometry, but joined by hydrogen bonding patterns different from those that join standard A:T and G:C pairs. Thus, this invention relates to nucleotide analogs and their derivatives that, when incorporated into DNA and RNA, expand the number of replicatable nucleotides beyond the four found in standard DNA and RNA. The invention further relates to polymerases that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives, and more specifically, polymerases and non-standard nucleoside triphosphates that support the polymerase chain reaction (PCR), including PCR where the products contain more than one non-standard nucleotide unit.
摘要翻译：所公开的发明教导了通过使模板和引物与DNA聚合酶和非标准核苷酸的三磷酸接触来扩增寡核苷酸的过程，其形成适合标准Watson-Crick几何形状的核碱基对，但是通过与加入标准A： T和G：C对。因此，本发明涉及核苷酸类似物及其衍生物，其在掺入DNA和RNA时扩大超出标准DNA和RNA中发现的四种可重复核苷酸的数量。本发明还涉及使用相应的三磷酸衍生物，更具体地，支持聚合酶链式反应（PCR）的聚合酶和非标准核苷三磷酸（包括产物含有的PCR）的寡核苷酸产物，将这些非标准核苷酸类似物结合到聚合酶中的聚合酶多个非标准核苷酸单位。

59. 发明申请

US20130330734A1 CONTROLLED INHIBITION AND RE-ACTIVATION OF DNA POLYMERASES BY CLEAVABLE OLIGONUCLEOTIDE INHIBITORS 有权
标题翻译：可溶性寡核苷酸抑制剂控制DNA聚合酶的抑制和再活化
公开(公告)号：US20130330734A1
公开(公告)日：2013-12-12
申请号：US13914745
申请日：2013-06-11
申请人： SAMSUNG TECHWIN CO., LTD.
发明人： Win D. CHEUNG , Jason A. OPDYKE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6848 , C12Q2521/101 , C12Q2521/307 , C12Q2527/127 , C12Q2549/101 , C12Q2521/327
摘要： A hot start enzyme composition is described that includes a hot start nuclease, a nucleic acid polymerase, and a substantially double-stranded oligonucleotide that inhibits the catalytic activity of the nucleic acid polymerase at temperatures lower than the melting temperature of the oligonucleotide.
摘要翻译：描述了一种热启动酶组合物，其包括热启动核酸酶，核酸聚合酶和基本上双链的寡核苷酸，其在低于寡核苷酸的解链温度的温度下抑制核酸聚合酶的催化活性。

60. 发明申请

US20130244340A1 Nanopore Based Molecular Detection and Sequencing 审中-公开
标题翻译：基于纳米孔的分子检测和测序
公开(公告)号：US20130244340A1
公开(公告)日：2013-09-19
申请号：US13745688
申请日：2013-01-18
申请人： GENIA TECHNOLOGIES, INC.
发明人： Randall Davis , Roger Chen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , G01N33/48721 , C12Q2521/101 , C12Q2565/607
摘要： This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.
摘要翻译：本公开提供用于分子鉴定和使用纳米孔的聚合物（例如，核酸）测序的系统和方法。聚合物可以通过纳米孔或靠近纳米孔，聚合物的各种亚基可能影响流过纳米孔的电流。可以通过测量跨过纳米孔和/或膜施加的多个电压的电流来识别各种亚基。在一些情况下，标记的核苷酸的聚合会向纳米孔呈现标签分子，其可以通过测量施加在纳米孔和/或膜上的多个电压的电流来鉴定。本文还提供了用纳米孔对双链核酸分子的有义链和反义链进行测序的系统和方法，以及使用核糖核酸（RNA）速度突变分子来减缓核酸分子通过的方法或靠近纳米孔。

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