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1. 发明授权

US08691968B2 Compositions and methods for the protection of nucleophilic groups 有权
标题翻译：用于保护亲核基团的组合物和方法
公开(公告)号：US08691968B2
公开(公告)日：2014-04-08
申请号：US13001735
申请日：2009-07-02
申请人： Fei Mao , Xing Xin , Wai-Yee Leung
发明人： Fei Mao , Xing Xin , Wai-Yee Leung
IPC分类号： C07H21/00 , C12Q1/68 , B01L3/00
CPC分类号： C12Q1/6844 , C07K1/064 , C08H1/00 , C12N9/1252 , C12N9/127 , C12P19/34 , C12Q2521/101 , C12Q2521/119
摘要： The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention.
摘要翻译：本发明提供了与包含亲核基团的分子的保护和去保护有关的组合物，方法和试剂盒，例如耐热聚合酶的保护和去保护。还提供使用根据本发明保护的聚合酶进行核酸扩增的方法。

2. 发明申请

US20100317016A1 DYES AND LABELED MOLECULES 有权
标题翻译：染色体和标签分子
公开(公告)号：US20100317016A1
公开(公告)日：2010-12-16
申请号：US12820983
申请日：2010-06-22
申请人： Fei Mao , Wai-Yee Leung , Xing Xin
发明人： Fei Mao , Wai-Yee Leung , Xing Xin
IPC分类号： C12Q1/68 , G01N33/566 , C07H21/00
CPC分类号： C12Q1/6876 , C07D209/08 , C07D213/38 , C07D221/10 , C07D417/06 , C07D417/14 , C07D491/147 , C07H21/00 , C09B15/00 , C12Q1/6851 , C12Q1/686 , C12Q2563/173
摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
摘要翻译：提供二聚和三聚体核酸染料以及相关系统和方法。这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

3. 发明授权

US07378968B2 Detecting the position of an RFID attached asset 有权
标题翻译：检测RFID附属资产的位置
公开(公告)号：US07378968B2
公开(公告)日：2008-05-27
申请号：US11210925
申请日：2005-08-24
申请人： Hao B J Wang , Rong Yao Fu , Song Song , Xing Xin Gao , Zhe Xiang , Jian Huang
发明人： Hao B J Wang , Rong Yao Fu , Song Song , Xing Xin Gao , Zhe Xiang , Jian Huang
IPC分类号： G08B13/14
CPC分类号： G01S5/0018 , G01S5/02
摘要： Provides asset position detecting apparatus and methods for determining the correspondence relation between an asset attached to an RFID tag and the position in which the asset is located in a predetermined space range. An example apparatus includes: a movable RFID reader movable in the space range, for detecting whether the asset exists in its read range, and reading the RFID tag attached to the asset within its read range, to obtain the specific identification of the detected asset; and position determining means for determining the position where the reader is located, wherein, when said RFID reader detects that said asset exists in its read range during its movement, the obtained identification of the asset and the current position of the RFID reader are associatively stored in a database, so as to obtain the information regarding the position of said asset.
摘要翻译：提供资产位置检测装置和方法，用于确定附着于RFID标签的资产与资产所位于预定空间范围内的位置之间的对应关系。一种示例性装置包括：在空间范围内可移动的可移动RFID读取器，用于检测资产是否存在于其读取范围内，以及读取在其读取范围内附着于资产的RFID标签，以获得所检测资产的特定标识; 以及位置确定装置，用于确定读取器所在的位置，其中当所述RFID读取器在其移动期间检测到所述资产存在于其读取范围内时，所获得的资产的识别和RFID读取器的当前位置被相关联在数据库中，以获得关于所述资产的位置的信息。

4. 发明申请

US20050272053A1 Oligonucleotides labeled with a plurality of fluorophores 失效
标题翻译：用多个荧光团标记的寡核苷酸
公开(公告)号：US20050272053A1
公开(公告)日：2005-12-08
申请号：US10993625
申请日：2004-11-19
申请人： Fei Mao , Xing Xin
发明人： Fei Mao , Xing Xin
IPC分类号： C07H20060101 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y10S435/975 , C12Q2565/1015 , C12Q2561/101 , C12Q2565/1025
摘要： An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5′-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.
摘要翻译：本发明的一个实施方案公开了通过用多个光谱相同或相似的染料和任选地与一种或多种猝灭剂染料标记寡核苷酸来设计标记的核酸探针和引物的新方法。根据本发明的一些实施方案标记的寡核苷酸在标记染料彼此分离时表现出可检测的信号增加，例如荧光信号。用于分离染料的方法包括切割标记的寡核苷酸包括使用具有5'-外切核酸酶活性的酶。在一个实施方案中，本发明的核酸引物可以在与靶序列杂交时发荧光，并掺入扩增产物。本发明的核酸探针和引物具有广泛的应用范围，从靶核酸序列的一般检测到临床诊断。包括本发明许多实施方案的核酸探针和引物的寡核苷酸的主要优点是它们的合成简便性，光谱通用性和优异的荧光信号。

5. 发明授权

US08530194B2 Oligonucleotides as temperature-sensitive inhibitors for DNA polymerases 失效
标题翻译：寡核苷酸作为DNA聚合酶的温度敏感抑制剂
公开(公告)号：US08530194B2
公开(公告)日：2013-09-10
申请号：US12088213
申请日：2006-09-26
申请人： Fei Mao , Xing Xin
发明人： Fei Mao , Xing Xin
IPC分类号： C12P19/34 , G01N33/53
CPC分类号： C12Q1/6848 , C12Q2527/101 , C12Q2525/205
摘要： Aspects of the invention relate to the use of novel oligonucleotides as temperature-sensitive inhibitors for thermostable DNA polymerases. Some inhibitors exhibit temperature-dependent and, in some cases, reversible inhibitory property by changing the conformation of at least a portion of the oligonucleotides from double-stranded to single stranded or in some cases vice versa in a temperature-dependent manner. Aspects also relate to the use of an the inhibitors in a hot-start PCR compositions, wherein the inhibitor may act to suppress the activity of the thermostable DNA polymerase below a desired activation temperature, Tact, and wherein the inhibitor is thermally inactivated above Tact, thus liberating the polymerase activity and initiating the DNA amplification process. Aspects further relate to a procedure for formulating the composition of a hot-start PCR reaction mixture. The hot-start PCR methods disclosed herein are generally faster, more flexible and lower in cost than existing methods.
摘要翻译：本发明的方面涉及新型寡核苷酸作为热稳定性DNA聚合酶的温度敏感性抑制剂的用途。一些抑制剂在温度依赖性方面通过将寡核苷酸的至少一部分构型从双链改变为单链或在一些情况下反之亦然地表现出温度依赖性，并且在一些情况下是可逆抑制性质。方面还涉及在热启动PCR组合物中使用抑制剂，其中抑制剂可以起作用以将热稳定性DNA聚合酶的活性抑制在所需活化温度Tact以下，并且其中抑制剂在Tact之上热灭活，从而释放聚合酶活性并引发DNA扩增过程。方面还涉及用于配制热启动PCR反应混合物的组合物的方法。本文公开的热启动PCR方法通常比现有方法更快，更灵活并且成本更低。

6. 发明申请

US20100285468A1 DETECTION AND/OR QUANTIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：检测和/或定量核酸
公开(公告)号：US20100285468A1
公开(公告)日：2010-11-11
申请号：US12679534
申请日：2008-09-24
申请人： Xing Xin
发明人： Xing Xin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6886 , C12Q2600/156 , C12Q2565/632 , C12Q2563/173 , C12Q2545/101 , C12Q2531/113 , C12Q2527/107
摘要： The present invention provides compositions, methods, and kits for nucleic acids analyses. In particular, melting analyses are used to detect the presence or absence and to quantify nucleic acids.
摘要翻译：本发明提供了用于核酸分析的组合物，方法和试剂盒。特别地，使用熔解分析来检测核酸的存在或不存在以及定量。

7. 发明授权

US07667024B2 Oligonucleotides labeled with a plurality of fluorophores 失效
标题翻译：用多个荧光团标记的寡核苷酸
公开(公告)号：US07667024B2
公开(公告)日：2010-02-23
申请号：US10993625
申请日：2004-11-19
申请人： Fei Mao , Xing Xin
发明人： Fei Mao , Xing Xin
IPC分类号： C07H21/02 , C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y10S435/975 , C12Q2565/1015 , C12Q2561/101 , C12Q2565/1025
摘要： An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5′-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.
摘要翻译：本发明的一个实施方案公开了通过用多个光谱相同或相似的染料和任选地与一种或多种猝灭剂染料标记寡核苷酸来设计标记的核酸探针和引物的新方法。根据本发明的一些实施方案标记的寡核苷酸在标记染料彼此分离时表现出可检测的信号增加，例如荧光信号。用于分离染料的方法包括切割标记的寡核苷酸包括使用具有5'-外切核酸酶活性的酶。在一个实施方案中，本发明的核酸引物可以在与靶序列杂交时发荧光，并掺入扩增产物。本发明的核酸探针和引物具有广泛的应用范围，从靶核酸序列的一般检测到临床诊断。包括本发明许多实施方案的核酸探针和引物的寡核苷酸的主要优点是它们的合成简便性，光谱通用性和优异的荧光信号。

8. 发明申请

US20090047714A1 OLIGONUCLEOTIDES AS TEMPERATURE-SENSITIVE INHIBITORS FOR DNA POLYMERASES 失效
标题翻译：寡核苷酸作为DNA聚合酶的温敏敏感抑制剂
公开(公告)号：US20090047714A1
公开(公告)日：2009-02-19
申请号：US12088213
申请日：2006-09-26
申请人： Fei Mao , Xing Xin
发明人： Fei Mao , Xing Xin
IPC分类号： C12P19/34 , C07H21/04 , C12N9/00
CPC分类号： C12Q1/6848 , C12Q2527/101 , C12Q2525/205
摘要： Aspects of the invention relate to the use of novel oligonucleotides as temperature-sensitive inhibitors for thermostable DNA polymerases. Some inhibitors exhibit temperature-dependent and, in some cases, reversible inhibitory property by changing the conformation of at least a portion of the oligonucleotides from double-stranded to single stranded or in some cases vice versa in a temperature-dependent manner. Aspects also relate to the use of an the inhibitors in a hot-start PCR compositions, wherein the inhibitor may act to suppress the activity of the thermostable DNA polymerase below a desired activation temperature, Tact, and wherein the inhibitor is thermally inactivated above Tact, thus liberating the polymerase activity and initiating the DNA amplification process. Aspects further relate to a procedure for formulating the composition of a hot-start PCR reaction mixture. The hot-start PCR methods disclosed herein are generally faster, more flexible and lower in cost than existing methods.
摘要翻译：本发明的方面涉及新型寡核苷酸作为热稳定性DNA聚合酶的温度敏感性抑制剂的用途。一些抑制剂在温度依赖性方面通过将寡核苷酸的至少一部分构型从双链改变为单链或在一些情况下反之亦然地表现出温度依赖性，并且在一些情况下是可逆抑制性质。方面还涉及在热启动PCR组合物中使用抑制剂，其中抑制剂可以起作用以将热稳定性DNA聚合酶的活性抑制在所需活化温度Tact以下，并且其中抑制剂在Tact之上热灭活，从而释放聚合酶活性并引发DNA扩增过程。方面还涉及用于配制热启动PCR反应混合物的组合物的方法。本文公开的热启动PCR方法通常比现有方法更快，更灵活并且成本更低。

9. 发明授权

US08530195B2 Dyes and labeled molecules 有权
标题翻译：染料和标记分子
公开(公告)号：US08530195B2
公开(公告)日：2013-09-10
申请号：US12820983
申请日：2010-06-22
申请人： Fei Mao , Wai-Yee Leung , Xing Xin
发明人： Fei Mao , Wai-Yee Leung , Xing Xin
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C07D209/08 , C07D213/38 , C07D221/10 , C07D417/06 , C07D417/14 , C07D491/147 , C07H21/00 , C09B15/00 , C12Q1/6851 , C12Q1/686 , C12Q2563/173
摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
摘要翻译：提供二聚和三聚体核酸染料以及相关系统和方法。这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

10. 发明申请

US20100086916A1 OLIGONUCLEOTIDE PROBES AND USES THEREOF 失效
标题翻译：寡核苷酸探针及其用途
公开(公告)号：US20100086916A1
公开(公告)日：2010-04-08
申请号：US12307358
申请日：2007-07-19
申请人： Fei Mao , Xing Xin
发明人： Fei Mao , Xing Xin
IPC分类号： C12Q1/68 , C07H21/00 , C12N9/10
CPC分类号： C07H19/04 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6851 , C12Q2561/101 , C12Q2527/107
摘要： The present invention provides a dually labeled oligonucleotide probe, methods of preparing and using the same. The subject probes are particularly useful for high-sensitive nucleic acid detection via hybridization assays including but not limited to template-directed polymerization reactions.
摘要翻译：本发明提供双重标记的寡核苷酸探针，其制备和使用方法。本发明探针通过杂交测定法对于高敏感性核酸检测特别有用，包括但不限于模板指导的聚合反应。

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