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1. 发明授权

US08722380B2 DNA polymerases with increased 3′-mismatch discrimination 有权
公开(公告)号：US08722380B2
公开(公告)日：2014-05-13
申请号：US13162688
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
IPC分类号： C12N9/00 , C12N15/54
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6858 , C12Q1/686 , C12Y207/07007 , C12Q2521/101
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

2. 发明申请

US20130196382A1 DNA POLYMERASES WITH IMPROVED ACTIVITY 有权
公开(公告)号：US20130196382A1
公开(公告)日：2013-08-01
申请号：US13558760
申请日：2012-07-26
申请人： Keith Bauer , Thomas W. Myers , Fred Reichert , Shawn Suko
发明人： Keith Bauer , Thomas W. Myers , Fred Reichert , Shawn Suko
IPC分类号： C12N9/12
CPC分类号： C12N9/1276 , C12N9/1252 , C12N15/1096 , C12Q1/686 , C12Y207/07049
摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

3. 发明授权

US06668106B1 Crosspoint microwave switching architecture for optical telecommunications 失效
标题翻译：光通信交叉点微波交换架构
公开(公告)号：US06668106B1
公开(公告)日：2003-12-23
申请号：US09792287
申请日：2001-02-23
申请人： Jules D. Levine , Thomas W. Myers , Christopher Weller
发明人： Jules D. Levine , Thomas W. Myers , Christopher Weller
IPC分类号： G02B626
CPC分类号： H04Q11/0005 , G02B6/356 , H04J14/0212 , H04Q2011/0035
摘要： The present invention combines several features to create a novel type of optical telecommunications switching component using microwave waveguides as opposed to the usual methods employing light deflection or digital signal processing. Some of the components of the device include: (1) an optical crossconnect (OXC) which allows any combination of wavelengths to be added or dropped from an element to an optical network, (2) an optical add/drop multiplexer (OADM) which allows any wavelength to be converted to any other, (3) a microwave crossbar switch array optimized for this purpose, and (4) a means for reshaping the exiting signal from the microwave crosspoint switch array without regard to signal format and without need for complex and expensive digital signal processing. The minimal architecture for this functionality is shown to be three N×N microwave crossbar switch arrays.
摘要翻译：与使用光偏转或数字信号处理的通常方法相反，本发明组合了若干特征以创建使用微波波导的新型光通信开关部件。器件的一些部件包括：（1）光学交叉连接（OXC），其允许将波长的任何组合从元件添加或掉落到光网络;（2）光分插复用器（OADM），其中允许将任何波长转换为任何其他（3）为此目的而优化的微波交叉开关阵列，以及（4）用于重新形成来自微波交叉点开关阵列的退出信号的装置，而不考虑信号格式，并且不需要复杂和昂贵的数字信号处理。该功能的最小架构显示为三个NxN微波交叉开关阵列。

4. 发明授权

US5618703A Unconventional nucleotide substitution in temperature selective RT-PCR 失效
标题翻译：温度选择性RT-PCR中的非常规核苷酸取代
公开(公告)号：US5618703A
公开(公告)日：1997-04-08
申请号：US199509
申请日：1994-02-22
申请人： David H. Gelfand , Thomas W. Myers
发明人： David H. Gelfand , Thomas W. Myers
IPC分类号： C12N9/12 , C12N15/70 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/703 , C12N15/70 , C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6876
摘要： Methods are provided for distinguishing between RNA and DNA templates in an amplification reaction. In a preferred embodiment of the invention, the amplification reaction is a PCR and the reaction is catalyzed by a thermostable DNA polymerase or both reverse transcription and amplification of a target RNA. The invention particularly relates to selective amplification of RNA in the presence of homologous DNA, for example, HIV nucleic acids.
摘要翻译：提供了用于区分扩增反应中的RNA和DNA模板的方法。在本发明的一个优选实施方案中，扩增反应是PCR，并且反应由热稳定的DNA聚合酶或靶向RNA的逆转录和扩增两者催化。本发明特别涉及在同源DNA例如HIV核酸存在下的RNA的选择性扩增。

5. 发明授权

US5561058A Methods for coupled high temperatures reverse transcription and polymerase chain reactions 失效
标题翻译：用于偶联高温逆转录和聚合酶链反应的方法
公开(公告)号：US5561058A
公开(公告)日：1996-10-01
申请号：US449050
申请日：1995-05-24
申请人： David H. Gelfand , Thomas W. Myers , Christopher L. Sigua
发明人： David H. Gelfand , Thomas W. Myers , Christopher L. Sigua
IPC分类号： C12N9/12 , C12N15/70 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C12N9/1252 , C12N15/70 , C12N9/1276 , C12P19/34 , C12Q1/6848 , C12Q1/686
摘要： Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
摘要翻译：提供了通过热活性DNA聚合酶复制和扩增RNA序列的方法。在优选的实施方案中，将高温逆转录与一个管中的核酸扩增相结合，一种使用热稳定DNA聚合酶的酶程序。还提供了用于消除由于先前的逆转录反应引起的扩增污染的方法。提供特别适用于本发明方法的试剂。

6. 发明授权

US5310652A Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription 失效
标题翻译：用热稳定DNA聚合酶 - 高温逆转录逆转录
公开(公告)号：US5310652A
公开(公告)日：1994-05-10
申请号：US82182
申请日：1993-06-24
申请人： David H. Gelfand , Thomas W. Myers
发明人： David H. Gelfand , Thomas W. Myers
IPC分类号： C12N9/12 , C12N15/70 , C12P19/34 , C12Q1/68 , C12Q1/70 , C07H5/04 , C07H15/12
CPC分类号： C12Q1/703 , C12N15/70 , C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6827 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6876
摘要： Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
摘要翻译：提供了通过热活性DNA聚合酶复制和扩增RNA序列的方法。在优选的实施方案中，将高温逆转录与一个管中的核酸扩增相结合，一种使用热稳定DNA聚合酶的酶程序。还提供了用于消除由于先前的逆转录反应引起的扩增污染的方法。

7. 发明授权

US09090883B2 DNA polymerases with improved activity 有权
公开(公告)号：US09090883B2
公开(公告)日：2015-07-28
申请号：US13558760
申请日：2012-07-26
申请人： Keith Bauer , Thomas W. Myers , Fred Reichert , Shawn Suko
发明人： Keith Bauer , Thomas W. Myers , Fred Reichert , Shawn Suko
IPC分类号： C12N9/12 , C12N15/11 , C12N15/10
CPC分类号： C12N9/1276 , C12N9/1252 , C12N15/1096 , C12Q1/686 , C12Y207/07049
摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

8. 发明授权

US08735121B2 DNA polymerases with increased 3′-match discrimination 有权
公开(公告)号：US08735121B2
公开(公告)日：2014-05-27
申请号：US13162674
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
IPC分类号： C12N9/00 , C12N15/54
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/686 , C12Y207/07007
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

9. 发明授权

US08722379B2 DNA polymerases with increased 3′-mismatch discrimination 有权
公开(公告)号：US08722379B2
公开(公告)日：2014-05-13
申请号：US13162677
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
IPC分类号： C12N9/12 , C12N15/54
CPC分类号： C12N9/1252 , C12Q1/6806 , C12Y207/07007
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

10. 发明授权

US08163487B2 2′-terminator nucleotide-related methods and systems 有权
标题翻译： 2'-终止子核苷酸相关方法和系统
公开(公告)号：US08163487B2
公开(公告)日：2012-04-24
申请号：US13045427
申请日：2011-03-10
申请人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas W. Myers
发明人： David Harrow Gelfand , Fred Lawrence Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas W. Myers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/101 , C12Q2525/101 , C12Q2525/186
摘要： The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要翻译：本发明提供扩增引物核酸和测序靶核酸的方法。所述方法包括使用2'-终止子核苷酸来实现链终止。除了相关的反应混合物和试剂盒之外，本发明还提供计算机和计算机可读介质。

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