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1. 发明申请

US20110104760A1 METHODS AND COMPOSITIONS FOR IMPROVING EFFICIENCY OF NUCLEIC ACIDS AMPLIFICATION REACTIONS 有权
标题翻译：提高核酸扩增反应效率的方法和组合
公开(公告)号：US20110104760A1
公开(公告)日：2011-05-05
申请号：US12683950
申请日：2010-01-07
申请人： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
发明人： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
IPC分类号： C12P19/34 , C12N9/96 , C12N9/12 , C07K16/00 , C07H21/04
CPC分类号： C12P19/34 , C07K16/40 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2319/80 , C12N9/1252 , C12Q1/6804 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2521/101
摘要： The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
摘要翻译：本发明提供了提高核酸扩增反应效率的方法和组合物。本发明包括显示比野生型聚合酶增加的活性以及降低的外切核酸酶活性的杂合聚合酶。本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。

2. 发明授权

US08859205B2 Methods and compositions for improving efficiency of nucleic acids amplification reactions 有权
标题翻译：提高核酸扩增反应效率的方法和组合物
公开(公告)号：US08859205B2
公开(公告)日：2014-10-14
申请号：US12683950
申请日：2010-01-07
申请人： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
发明人： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
IPC分类号： C12Q1/68 , C12P19/34 , C07K1/00 , C12N9/12 , C07K16/40
CPC分类号： C12P19/34 , C07K16/40 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2319/80 , C12N9/1252 , C12Q1/6804 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2521/101
摘要： The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
摘要翻译：本发明提供了提高核酸扩增反应效率的方法和组合物。本发明包括显示比野生型聚合酶增加的活性以及降低的外切核酸酶活性的杂合聚合酶。本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。

3. 发明授权

US06897023B2 Method for determining relative abundance of nucleic acid sequences 失效
标题翻译：确定核酸序列相对丰度的方法
公开(公告)号：US06897023B2
公开(公告)日：2005-05-24
申请号：US09967238
申请日：2001-09-27
申请人： Rongdian Fu , Sydney Brenner , Glenn Albrecht
发明人： Rongdian Fu , Sydney Brenner , Glenn Albrecht
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2545/114 , C12Q2563/179 , C12Q2565/626
摘要： Disclosed are methods for identifying nucleic acid sequences which are of different abundances in different nucleic acid source populations, e.g. differentially expressed genes or genomic variations among individuals or populations of individuals. In one embodiment, probes derived from the source nucleic acid populations are derivatized with a terminal sample ID (SID) sequence characteristic of that population. Upon competitive hybridization of the probes to a reference or index nucleic acid library containing all the sequences in the populations being compared, the SID tags remain single stranded, and those from the different sources are then annealed to one another. Unhybridized (remainder) SID sequences are then quantified. By labeling such remainder SID sequences with a fluorescent dye, FACS sorting of beads containing the hybridized probes can be carried out. The signal ratio upon which such sorting is based is enhanced compared to competitive hybridization using labeled probes without SID sequences.
摘要翻译：公开了用于鉴定在不同核酸源群体中具有不同丰度的核酸序列的方法，例如，差异表达基因或个体个体或个体群体的基因组变异。在一个实施方案中，衍生自源核酸群体的探针用该群体特征的终端样品ID（SID）序列衍生化。当探针与包含正在比较的群体中的所有序列的参考或指数核酸文库竞争性杂交时，SID标签保持单链，然后来自不同来源的那些序列彼此退火。然后对未杂交（其余）SID序列进行定量。通过用荧光染料标记这些剩余的SID序列，可以进行含有杂交探针的珠的FACS分选。与使用没有SID序列的标记探针的竞争性杂交相比，这种分选所基于的信号比率增加。

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