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31. 发明申请

US20140206559A1 ASSAY FOR METASTATIC POTENTIAL OF TUMOR CELLS 审中-公开
标题翻译：肿瘤细胞分化潜能的测定
公开(公告)号：US20140206559A1
公开(公告)日：2014-07-24
申请号：US14119479
申请日：2012-05-23
申请人： George M. Whitesides , Ratmir Derda , Renee Fuller , Frederique T. Deiss
发明人： George M. Whitesides , Ratmir Derda , Renee Fuller , Frederique T. Deiss
IPC分类号： G01N33/483
CPC分类号： G01N33/4833 , G01N33/5091 , G01N33/574
摘要： The present invention relates to methods, compositions and kits related to a novel in vitro assay for a high-capacity and high-throughput method for measuring the ability of cancer cells to migrate in a three-dimensional cellular assay. The three-dimensional cellular invasion assay provides a method for determining and quantitating the metastatic potential and invasive capacity of a cancer cell. Other aspects of the invention further relate to the use of the in vitro assay to screen for agents and compounds capable of inhibiting intravasation, and thereby modulating the metastatic potential of cancer cells. The methods, compositions and three-dimensional assay provide a highly sensitive assay system capable of mimicking the in vivo cellular and molecular interactions required for successful completion of intravasation.
摘要翻译：本发明涉及与用于测量癌细胞在三维细胞测定中迁移的能力的高容量和高通量方法的新体外测定相关的方法，组合物和试剂盒。三维细胞侵袭测定提供了确定和定量癌细胞转移潜能和侵袭能力的方法。本发明的其它方面进一步涉及使用体外测定来筛选能够抑制渗透的药剂和化合物，从而调节癌细胞的转移潜力。方法，组合物和三维测定提供了能够模拟成功完成渗透所需的体内细胞和分子相互作用的高度灵敏的测定系统。

32. 发明申请

US20130280693A1 DENSITY-BASED SEPARATION OF BIOLOGICAL ANALYTES USING MULTIPHASE SYSTEMS 有权
公开(公告)号：US20130280693A1
公开(公告)日：2013-10-24
申请号：US13817985
申请日：2011-08-22
申请人： Charles R. Mace , Ashok A. Kumar , Dyann F. Wirth , George M. Whitesides
发明人： Charles R. Mace , Ashok A. Kumar , Dyann F. Wirth , George M. Whitesides
IPC分类号： G01N33/49 , G01N33/574
CPC分类号： G01N33/491 , B03B5/28 , B03B5/442 , B03D3/00 , G01N33/18 , G01N33/5375 , G01N33/57492
摘要： The disclosed methods use a multi-phase system to separate samples according to the density of an analyte of interest. The method uses a multi-phase system that comprises two or more phase-separated solutions and a phase component such as a surfactant or polymer. The density of the analyte of interest differs from the densities of the rest of the sample. The density of the analyte of interest is substantially the same as one or more phases. Thus, when the sample is introduced to the multi-phase system, the analyte of interest migrates to the phase having the same density as the analyte of interest, passing through one or more phases sequentially.

33. 发明授权

US08501416B2 Fluidic structures including meandering and wide channels 有权
标题翻译：流体结构包括曲折和宽通道
公开(公告)号：US08501416B2
公开(公告)日：2013-08-06
申请号：US11887487
申请日：2006-04-19
申请人： Vincent Linder , Samuel K. Sia , George M. Whitesides , Max Narovlyansky , Adam Siegel
发明人： Vincent Linder , Samuel K. Sia , George M. Whitesides , Max Narovlyansky , Adam Siegel
IPC分类号： G01N33/53
CPC分类号： G01N33/54366 , B01L3/502746 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0883 , B01L2400/0406 , G01N21/01 , G01N21/82 , G01N33/54373
摘要： The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analysis due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area. Although this invention mainly describes the use of meandering and wide channels in heterogeneous immunoassays on a microfluidic chip, this invention could be used for amplifying optical signals for other types of reactions and/or assays.
摘要翻译：本发明一般涉及微流体结构，更具体地涉及包括曲折和宽通道的微流体结构和方法。微流控系统可以提供用于执行各种反应和分析的有利环境，因为与常规系统相比，所需的样品和试剂量的减少，操作系统的尺寸的减小和反应时间的减少。不幸的是，微量流体通道的小尺寸有时会导致在不放大光学器件（例如显微镜或光电倍增管）的情况下检测物质的困难。一系列紧密包装的微通道，即曲折区域或具有大约毫米数量级的宽通道可以通过创建宽的测量区域来解决这个问题。尽管本发明主要描述了在微流体芯片上的异源免疫测定中使用曲折和宽通道，但是本发明可用于放大用于其他类型反应和/或测定的光学信号。

34. 发明授权

US08124743B2 Purification of a bivalently active antibody using a non-chromatographic method 有权
标题翻译：使用非色谱法纯化二价活性抗体
公开(公告)号：US08124743B2
公开(公告)日：2012-02-28
申请号：US12303089
申请日：2007-06-01
申请人： Vijay M. Krishnamurthy , Lara A. Estroff , Vincent Semetey , Samuel W. Thomas , George K. Kaufman , Zihni Basar Bilgicer , George M. Whitesides
发明人： Vijay M. Krishnamurthy , Lara A. Estroff , Vincent Semetey , Samuel W. Thomas , George K. Kaufman , Zihni Basar Bilgicer , George M. Whitesides
IPC分类号： C07K16/00 , C07K1/22 , C07K1/30
CPC分类号： C07K16/065 , C07K16/468 , C07K2317/31 , C07K2317/54 , Y10S436/822
摘要： The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment.
摘要翻译：本发明公开了一种纯化二价抗体或抗体片段的方法，所述二价抗体或抗体片段在使用非色谱法的抗体或抗体片段来源的两个Fab位点都是活性的，其包括通过将多价半抗原加入到可溶性抗体或抗体片段的盐溶液，其中多价半抗原具有两个半抗原之间的接头，其有效地防止配体的半抗原与相同的抗体或抗体片段的结合。

35. 发明申请

US20110315229A1 FLUID DELIVERY SYSTEM AND METHOD 有权
标题翻译：流体输送系统和方法
公开(公告)号：US20110315229A1
公开(公告)日：2011-12-29
申请号：US13226154
申请日：2011-09-06
申请人： Vincent Linder , Samuel K. Sia , George M. Whitesides
发明人： Vincent Linder , Samuel K. Sia , George M. Whitesides
IPC分类号： F15D1/00
CPC分类号： G01N33/5304 , B01L3/0293 , B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2200/16 , B01L2300/0816 , B82Y30/00 , G01N33/543 , G01N35/08 , G01N35/1002 , G01N35/1065 , Y10T137/0318
摘要： A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.
摘要翻译：一种用于递送一种或多种流体的方法和装置。流体可以从普通容器依次递送到化学，生物或生化过程。

36. 发明授权

US07993905B2 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US07993905B2
公开(公告)日：2011-08-09
申请号：US12166287
申请日：2008-07-01
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
IPC分类号： C12N1/00 , C12N11/00 , C12N11/08
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

37. 发明申请

US20110189786A1 COTTON THREAD AS A LOW-COST MULTI-ASSAY DIAGNOSTIC PLATFORM 有权
标题翻译：棉线作为低成本多测量诊断平台
公开(公告)号：US20110189786A1
公开(公告)日：2011-08-04
申请号：US12934702
申请日：2009-03-27
申请人： Meital Reches , Michael D. Dickey , Manish J. Butte , George M. Whitesides
发明人： Meital Reches , Michael D. Dickey , Manish J. Butte , George M. Whitesides
IPC分类号： G01N21/78 , C40B60/12
CPC分类号： B01L3/5023 , B01L3/5088 , B01L2200/0605 , B01L2200/10 , B01L2300/0825 , B01L2300/0845 , B01L2300/087 , B01L2300/12 , B01L2300/161 , B01L2400/0406 , G01N33/54386
摘要： Hydrophilic threads as platforms for inexpensive, low volume, portable diagnostic systems, and methods of making the same are described. A diagnostic system includes a hydrophilic loading thread having an inlet zone at a proximal end; a testing zone at a distal end; and an intermediate zone located between the inlet zone and the testing zone, wherein the testing zone does not directly contact the inlet zone. In another aspect, a diagnostic system includes (i) a hydrophilic loading thread that includes an inlet zone at a proximal end and an intermediate zone at a distal end; and (ii) one or more additional hydrophilic threads that contact the intermediate zone of the loading thread. A method of detecting the presence or absence of an analyte in a fluid sample includes applying the sample to an inlet zone of a diagnostic system that includes a hydrophilic loading thread with an inlet zone at a proximal end; an intermediate zone; and a testing zone at a distal end; wherein the testing zone does not directly contact the inlet zone.
摘要翻译：描述了作为廉价，低容量，便携式诊断系统的平台的亲水性线，以及制造它们的方法。诊断系统包括在近端具有入口区域的亲水加载螺纹; 远端的测试区; 以及位于入口区域和测试区域之间的中间区域，其中测试区域不直接接触入口区域。在另一方面，诊断系统包括（i）亲水加载线，其包括在近端处的入口区域和在远端处的中间区域; 和（ii）接触加载螺纹的中间区域的一个或多个附加亲水丝线。检测流体样品中分析物的存在或不存在的方法包括将样品施加到诊断系统的入口区域，诊断系统的入口区域包括具有近端的入口区域的亲水加载螺纹; 中间区和远端的测试区; 其中所述测试区域不直接接触所述入口区域。

38. 发明授权

US07741014B2 Patterning and alteration of molecules 有权
标题翻译：分子的形成和改变
公开(公告)号：US07741014B2
公开(公告)日：2010-06-22
申请号：US11401485
申请日：2006-04-10
申请人： Declan Ryan , Babak Amir-Parviz , Vincent Linder , Vincent Semetey , Samuel K. Sia , George M. Whitesides
发明人： Declan Ryan , Babak Amir-Parviz , Vincent Linder , Vincent Semetey , Samuel K. Sia , George M. Whitesides
IPC分类号： G03F7/00 , H01L21/302
CPC分类号： G03F7/165 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B82Y5/00 , B82Y30/00 , C03C17/28 , C03C23/0005 , C03C2218/32 , C40B80/00 , G03F7/203
摘要： A series of methods, compositions, and articles for patterning a surface with multiple, aligned layers of molecules, by exposing the molecules to electromagnetic radiation is provided. In certain embodiments, a single photomask acts as an area-selective filter for light at multiple wavelengths. A single set of exposures of multiple wavelengths through this photomask may make it possible to fabricate a pattern comprising discontinuous multiple regions, where the regions differ from each other in at least one chemical and/or physical property, without acts of alignment between the exposures. In certain embodiments, the surface includes molecules attached thereto that can be photocleaved upon exposure to a certain wavelength of radiation, thereby altering the chemical composition on at least a portion of the surface.
摘要翻译：提供了一系列用于通过将分子暴露于电磁辐射来图案化具有多个对准的分子层的表面的方法，组合物和制品。在某些实施例中，单个光掩模用作多个波长的光的区域选择滤光器。通过该光掩模的多组波长的一组曝光可以使得可以制造包括不连续多个区域的图案，其中区域在至少一种化学和/或物理性质上彼此不同，而不影响曝光之间的对准。在某些实施方案中，表面包括附着于其上的分子，其可以在暴露于一定波长的辐射时被光刻，从而改变表面的至少一部分上的化学组成。

39. 发明授权

US07724541B2 Self-assembled electrical networks 有权
标题翻译：自组装电网
公开(公告)号：US07724541B2
公开(公告)日：2010-05-25
申请号：US11352923
申请日：2006-02-13
申请人： David H. Gracias , Joe Tien , George M. Whitesides
发明人： David H. Gracias , Joe Tien , George M. Whitesides
IPC分类号： H05K7/06 , H01L49/00
CPC分类号： B82Y10/00 , B82Y30/00 , H01L25/50 , H01L2224/95122 , H01L2224/95144 , H01L2224/95145 , H01L2924/01019 , H01L2924/0102 , H01L2924/12041 , Y10T29/49002 , Y10T29/49128 , Y10T29/4913 , Y10T29/49155 , Y10T29/49169 , Y10T29/49208 , Y10T29/4921
摘要： Techniques for self assembly of macro-scale objects, optionally defining electrical circuitry, are described, as well as articles formed by self assembly. Components can be joined, during self-assembly by minimization of free energy, capillary attraction, or a combination.
摘要翻译：描述用于大规模物体的自组装的技术，可选地限定电路，以及通过自组装形成的制品。组件可以在自组装过程中通过最小化自由能，毛细管吸引力或组合来结合。

40. 发明申请

US20090166903A1 MOLDED WAVEGUIDES 有权
标题翻译：模制波形
公开(公告)号：US20090166903A1
公开(公告)日：2009-07-02
申请号：US12398132
申请日：2009-03-04
申请人： Enoch Kim , Younan Xia , Milan Mrksich , Rebecca J. Jackman , Xiao-Mei Zhao , Stephen P. Smith , Mara G. Prentiss , George M. Whitesides , Christian Marzolin
发明人： Enoch Kim , Younan Xia , Milan Mrksich , Rebecca J. Jackman , Xiao-Mei Zhao , Stephen P. Smith , Mara G. Prentiss , George M. Whitesides , Christian Marzolin
IPC分类号： B29D11/00
CPC分类号： B81C1/00206 , A61K47/6957 , B01J19/0046 , B01J2219/00382 , B01J2219/00416 , B01J2219/0043 , B01J2219/00466 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00713 , B29C31/04 , B29C33/42 , B29C37/0053 , B29C39/24 , B29C43/021 , B29C67/246 , B29D11/00346 , B29D11/00663 , B29K2105/0002 , B29L2011/0016 , B81B2201/0214 , B81C1/00031 , B81C99/0085 , B81C2201/034 , B82Y30/00 , C40B60/14 , G01N21/7703 , G01N2021/7776 , G02B6/138 , H05K3/0079 , H05K3/061
摘要： Chemically or biochemically active agents or other species are patterned on a substrate surface by providing a micromold having a contoured surface and forming, on a substrate surface, a chemically or biochemically active agent or fluid precursor of a structure. A chemically or biochemically active agent or fluid precursor also can be transferred from indentations in an applicator to a substrate surface. The substrate surface can be planar or non-planar. Fluid precursors of polymeric structures, inorganic ceramics and salts, and the like can be used to form patterned polymeric articles, inorganic salts and ceramics, reactive ion etch masks, etc. at the surface. The articles can be formed in a pattern including a portion having a lateral dimension of less than about 1 millimeter or smaller. The indentation pattern of the applicator can be used to transfer separate, distinct chemically or biochemically active agents or fluid precursors to separate, isolated regions of a substrate surface. Waveguide arrays, combinatorial chemical or biochemical libraris, etc. can be made. Differences in refractive index of waveguide and cladding can be created by subjecting the waveguide and cladding, made of indentical prepolymeric material, to different polymerization or cross-linking conditions. Interferometers are defined by coupling arrays of waveguides, where coupling can be controlled by altering the difference in refractive index between cladding and waveguide at any desired location of the array. Alteration and refractive index can be created photochemically, chemically, or the like. Sensors also are disclosed, including biochemical sensors.
摘要翻译：化学或生物化学活性剂或其它物质通过提供具有轮廓表面的微胶体并在基底表面上形成结构的化学或生物化学活性剂或流体前体而在基底表面上图案化。化学或生物化学活性剂或流体前体也可以从敷料器中的凹陷转移到基底表面。衬底表面可以是平面的或非平面的。聚合物结构，无机陶瓷和盐等的流体前体可用于在表面形成图案化聚合物制品，无机盐和陶瓷，反应离子蚀刻掩模等。制品可以形成为包括横向尺寸小于约1毫米或更小的部分的图案。涂抹器的压痕图案可以用于将单独的，不同的化学或生物化学活性剂或流体前体转移到基底表面的分离的，分离的区域。可以制作波导阵列，组合化学或生化图谱等。波导和包层的折射率差异可以通过将由凹凸预聚物材料制成的波导和包层进行不同的聚合或交联条件来产生。干涉仪通过耦合波导阵列来定义，其中可以通过改变阵列的任何所需位置处的包层和波导之间的折射率差来控制耦合。改变和折射率可以通过光化学，化学等方式产生。还公开了传感器，包括生化传感器。

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