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1. 发明授权

US07993905B2 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US07993905B2
公开(公告)日：2011-08-09
申请号：US12166287
申请日：2008-07-01
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
IPC分类号： C12N1/00 , C12N11/00 , C12N11/08
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

2. 发明授权

US07410791B2 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US07410791B2
公开(公告)日：2008-08-12
申请号：US11331849
申请日：2006-01-13
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
IPC分类号： C12N11/00 , C12N11/08 , C12N1/00
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

3. 发明申请

US20060210595A1 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
公开(公告)号：US20060210595A1
公开(公告)日：2006-09-21
申请号：US11331849
申请日：2006-01-13
申请人： Rahul Singhvi , Amit Kumar , George Whitesides , Donald Ingber , Gabriel Lopez , Daniel Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George Whitesides , Donald Ingber , Gabriel Lopez , Daniel Wang , Gregory Stephanopoulos
IPC分类号： A61F2/06 , A61F13/00
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

4. 发明授权

US5510262A Cell-culturing apparatus and method employing a macroporous support 失效
标题翻译：细胞培养装置和使用大孔支持物的方法
公开(公告)号：US5510262A
公开(公告)日：1996-04-23
申请号：US153204
申请日：1993-11-16
申请人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
发明人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
IPC分类号： C12M1/04 , C12M1/40 , C12M3/06 , C12N5/00 , C12M3/00
CPC分类号： C12M25/18 , C12M23/24 , C12M29/04 , C12M29/06 , C12N5/0068
摘要： An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.
摘要翻译：公开了一种用于通过使介质流过细胞培养反应器中的生物相容性大孔陶瓷颗粒的填充床来培养细胞的装置和方法。在一个实施方案中，培养基中的氧和其他营养物质通过生物相容性大孔陶瓷颗粒的孔对流流动被显着转运到设置在孔内的细胞中。可以通过将氧气喷射到细胞培养反应器中来将氧气引入细胞培养反应器。培养基中的氧含量也可以通过增加介质中氧的溶解度来增加，例如通过向培养基中加入全氟化碳或其它氧载体。通过在填充床中的多个点处将含氧气体喷射到介质中，或者通过将氧气引入到介质中，通过设置在所述填充床中的固相氧气可渗透膜，可以进一步增加反应器的氧气容量包装床或者，可以将含氧气体引导通过设置在生物相容性大孔载体的至少一个通道内的透氧导管。

5. 发明授权

US5262320A Cell-culturing apparatus and method employing a macroporous support 失效
标题翻译：细胞培养装置和使用大孔支持物的方法
公开(公告)号：US5262320A
公开(公告)日：1993-11-16
申请号：US921920
申请日：1992-07-29
申请人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
发明人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
IPC分类号： C12M1/04 , C12M1/40 , C12M3/06 , C12N5/00 , A01N1/02 , C12M3/00
CPC分类号： C12M25/18 , C12M23/24 , C12M29/04 , C12M29/06 , C12N5/0068
摘要： An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.
摘要翻译：公开了一种用于通过使介质流过细胞培养反应器中的生物相容性大孔陶瓷颗粒的填充床来培养细胞的装置和方法。在一个实施方案中，培养基中的氧和其他营养物质通过生物相容性大孔陶瓷颗粒的孔对流流动被显着转运到设置在孔内的细胞中。可以通过将氧气喷射到细胞培养反应器中来将氧气引入细胞培养反应器。培养基中的氧含量也可以通过增加介质中氧的溶解度来增加，例如通过向培养基中加入全氟化碳或其它氧载体。通过在填充床中的多个点处将含氧气体喷射到介质中，或者通过将氧气引入到介质中，通过设置在所述填充床中的固相氧气可渗透膜，可以进一步增加反应器的氧气容量包装床或者，可以将含氧气体引导通过设置在生物相容性大孔载体的至少一个通道内的透氧导管。

6. 发明授权

US09284570B2 Microbial production of natural sweeteners, diterpenoid steviol glycosides 有权
标题翻译：微生物生产天然甜味剂，二萜类甜菊糖苷
公开(公告)号：US09284570B2
公开(公告)日：2016-03-15
申请号：US13306633
申请日：2011-11-29
申请人： Gregory Stephanopoulos , Parayil K. Ajikumar
发明人： Gregory Stephanopoulos , Parayil K. Ajikumar
IPC分类号： C12P15/00 , C12N9/90 , C12N9/88 , C12N9/10 , C12N9/02 , C12N9/12 , C12N15/00 , C12N1/20 , C07H21/04 , C12N15/82 , C12N15/70 , A01H5/00 , A01H13/00 , A23L1/236 , A23L2/60 , C12P7/42 , C12P17/02
CPC分类号： C12N15/8243 , A01H5/00 , A01H13/00 , A23L2/60 , A23L27/36 , C12N9/0042 , C12N9/0073 , C12N9/0085 , C12N9/1085 , C12N9/1288 , C12N9/88 , C12N9/90 , C12N15/70 , C12P7/42 , C12P15/00 , C12P17/02 , C12Y106/02004 , C12Y114/13 , C12Y114/13076 , C12Y114/13078 , C12Y114/99009 , C12Y205/01029 , C12Y402/03019 , C12Y505/01012 , C12Y505/01013 , Y02P20/52
摘要： The invention relates to recombinant expression of a steviol or steviol glycosides biosynthetic pathway enzymes in cells and the production of steviol or steviol glycosides.
摘要翻译：本发明涉及细胞中甜菊醇或甜菊糖苷苷合成途径酶的重组表达，以及甜菊醇或甜菊糖苷的生产。

7. 发明授权

US08512988B2 Microbial engineering for the production of chemical and pharmaceutical products from the isoprenoid pathway 有权
标题翻译：用于从类异戊二烯途径生产化学和药物产品的微生物工程
公开(公告)号：US08512988B2
公开(公告)日：2013-08-20
申请号：US12943477
申请日：2010-11-10
申请人： Parayil K. Ajikumar , Gregory Stephanopoulos , Heng Phon Too
发明人： Parayil K. Ajikumar , Gregory Stephanopoulos , Heng Phon Too
IPC分类号： C12P5/00 , C12P5/02 , C12Q1/02 , C12N15/74 , C12N1/20
CPC分类号： C12N15/70 , C12P15/00 , C12P17/02
摘要： The invention relates to recombinant expression of a taxadiene synthase enzyme and a geranylgeranyl diphosphate synthase (GGPPS) enzyme in cells and the production of terpenoids.
摘要翻译：本发明涉及一种紫杉二烯合酶和一种香叶基香叶基二磷酸合成酶（GGPPS）在细胞中的表达和萜类化合物的产生。

8. 发明申请

US20120164678A1 MICROBIAL PRODUCTION OF NATURAL SWEETENERS, DITERPENOID STEVIOL GLYCOSIDES 有权
标题翻译：微生物生产天然维生素DITERPENOID STEVIOL GLYCOSIDES
公开(公告)号：US20120164678A1
公开(公告)日：2012-06-28
申请号：US13306633
申请日：2011-11-29
申请人： Gregory Stephanopoulos , Parayil K. Ajikumar
发明人： Gregory Stephanopoulos , Parayil K. Ajikumar
IPC分类号： C12Q1/02 , C12P7/40 , C07H21/04 , C12N9/00 , C12N1/21 , C12N1/19 , C12P19/56 , C12P5/00
CPC分类号： C12N15/8243 , A01H5/00 , A01H13/00 , A23L2/60 , A23L27/36 , C12N9/0042 , C12N9/0073 , C12N9/0085 , C12N9/1085 , C12N9/1288 , C12N9/88 , C12N9/90 , C12N15/70 , C12P7/42 , C12P15/00 , C12P17/02 , C12Y106/02004 , C12Y114/13 , C12Y114/13076 , C12Y114/13078 , C12Y114/99009 , C12Y205/01029 , C12Y402/03019 , C12Y505/01012 , C12Y505/01013 , Y02P20/52
摘要： The invention relates to recombinant expression of a steviol or steviol glycosides biosynthetic pathway enzymes in cells and the production of steviol or steviol glycosides.
摘要翻译：本发明涉及细胞中甜菊醇或甜菊糖苷苷合成途径酶的重组表达，以及甜菊醇或甜菊糖苷的生产。

9. 发明授权

US08062878B2 Methods for microbial production of terpenoids 失效
标题翻译：萜类化合物微生物生产方法
公开(公告)号：US08062878B2
公开(公告)日：2011-11-22
申请号：US12615985
申请日：2009-11-10
申请人： Effendi Leonard , Parayil K. Ajikumar , Kristala Lanett Jones Prather , Gregory Stephanopoulos , Kelly Thayer
发明人： Effendi Leonard , Parayil K. Ajikumar , Kristala Lanett Jones Prather , Gregory Stephanopoulos , Kelly Thayer
IPC分类号： C12N1/00 , C12N1/20 , C12P1/00
CPC分类号： C12N9/88 , C12N9/1085 , C12P15/00 , C12Y205/01029
摘要： The invention relates to recombinant expression of terpenoid synthase enzymes and geranylgeranyl diphosphate synthase (GGPPS) enzymes in cells and the production of diterpenoids.
摘要翻译：本发明涉及细胞中萜类合酶和香叶基香叶基二磷酸合成酶（GGPPS）酶的重组表达和二萜类化合物的生成。

10. 发明授权

US07739055B2 Methods and systems for generating and evaluating peptides 失效
标题翻译：用于产生和评估肽的方法和系统
公开(公告)号：US07739055B2
公开(公告)日：2010-06-15
申请号：US11561266
申请日：2006-11-17
申请人： Gregory Stephanopoulos , Kyle Jensen , Christopher Loose
发明人： Gregory Stephanopoulos , Kyle Jensen , Christopher Loose
IPC分类号： G06F19/00
CPC分类号： C07K7/08 , A61K38/00 , C07K14/001 , C07K14/4723 , C40B50/02 , G06F19/18 , G06F19/22 , G06F19/24 , G06F19/28
摘要： Methods to create databases of peptides having a desirable property, such as antimicrobial activity, involving analyzing a database of known peptides for a pattern statistically associated with the desirable property are described herein, The set of sequences being analyzed may include sequences of a desired length containing all or substantially all combinations of amino acids that conform to at least one of the set of patterns. Once the database is identified, the database may be processed in a pattern recognition procedure that identifies a set of patterns that may be representative of a peptide having the desirable property. A set of newly generated peptides sequences may then be processed to score these new sequences against the identified patterns to correlate the patterns to the sequences and determine a degree of association or similarity between one or more of the new sequences and the set of identified patterns.
摘要翻译：本文描述了创建具有期望性质（例如抗微生物活性）的肽的数据库的方法，其涉及分析已知肽的数据库以获得与所需性质统计学相关的模式。所分析的一组序列可以包含期望长度的序列，其含有所有或基本上所有符合该组图案中的至少一个的氨基酸组合。一旦识别了数据库，就可以在模式识别程序中处理数据库，该模式识别程序识别可以代表具有所需性质的肽的一组模式。然后可以处理一组新产生的肽序列以针对所识别的模式对这些新序列进行评估，以将模式与序列相关联，并确定一个或多个新序列与所识别的模式集合之间的关联度或相似度。

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