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31. 发明申请

US20120045748A1 Particulate labels 审中-公开
标题翻译：微粒标签
公开(公告)号：US20120045748A1
公开(公告)日：2012-02-23
申请号：US13135364
申请日：2011-06-30
申请人： Richard C. Willson , Ulrich Strych , Binh V. Vu
发明人： Richard C. Willson , Ulrich Strych , Binh V. Vu
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6804 , C12Q1/70 , C12Q2522/101 , C12Q2563/131 , C12Q2563/179
摘要： A methodology for bioassays and diagnostics in which a particulate label (ranging in size from nm-scale molecular assemblages to organisms on the scale of tens or hundreds of microns), such as, but not limited to, nanoparticles, bacteria, bacteriophage, Daphnia, and magnetic particles, serve carriers for analytes bound by molecular recognition elements such as antibodies, aptamers, etc. The described methodology is generally applicable to most pathogen assays and molecular diagnostics and also leads to enhanced sensitivity and convenience of use.
摘要翻译：用于生物测定和诊断的方法，其中颗粒标签（尺寸范围从纳米级分子组合到几十或数百微米的尺度的生物体），例如但不限于纳米颗粒，细菌，噬菌体，水蚤，和磁性颗粒，为分子识别元件（例如抗体，适配体等）所结合的分析物提供载体。所描述的方法通常适用于大多数病原体测定和分子诊断，并且还导致增强的灵敏度和使用方便性。

32. 发明申请

US20110257382A1 Nucleic acid separation using immobilized metal affinity chromatography 审中-公开
标题翻译：使用固定化金属亲和色谱法进行核酸分离
公开(公告)号：US20110257382A1
公开(公告)日：2011-10-20
申请号：US12584889
申请日：2009-09-14
申请人： Richard C. Willson , Jason C. Murphy
发明人： Richard C. Willson , Jason C. Murphy
IPC分类号： C07H1/06
CPC分类号： C12N15/101
摘要： An immobilized metal affinity chromatography (IMAC) method for separating and/or purifying compounds containing a non-shielded purine or pyrimidine moiety or group such as nucleic acid, presumably through interaction with the abundant aromatic nitrogen atoms in the purine or pyrimidine moiety. The method can also be used to purify compounds containing purine or pyrimidine moieties where the purine and pyrimidine moieties are shielded from interaction with the column matrix from compounds containing a non-shielded purine or pyrimidine moiety or group. Thus, double-stranded plasmid and genomic DNA, which has no low binding affinity can be easily separated from RNA and/or oligonucleotides which bind strongly to metal-charged chelating matrices. IMAC columns clarify plasmid DNA from bacterial alkaline lysates, purify a ribozyme, and remove primers and other contaminants from PCR reactions. The metal ion affinity of yeast RNA decreases in the order: copper (II), nickel (II), zinc (II), and cobalt (II).
摘要翻译：用于分离和/或纯化含有非屏蔽嘌呤或嘧啶部分或核酸的化合物的固定化金属亲和层析（IMAC）方法，可能通过与嘌呤或嘧啶部分中丰富的芳族氮原子相互作用来进行。该方法还可用于纯化含有嘌呤或嘧啶部分的化合物，其中嘌呤和嘧啶部分被屏蔽以免与含有非屏蔽嘌呤或嘧啶部分或基团的化合物与柱基质相互作用。因此，具有低结合亲和力的双链质粒和基因组DNA可以容易地从与金属电荷螯合基质强结合的RNA和/或寡核苷酸中分离出来。 IMAC色谱柱澄清细菌碱性裂解物的质粒DNA，纯化核酶，并从PCR反应中除去引物和其他污染物。酵母RNA的金属离子亲和力按照铜（II），镍（II），锌（II）和钴（II）的顺序降低。

33. 发明申请

US20100210009A1 OPTICAL MICROLABELS: SHAPES AND REFLECTORS 失效
标题翻译：光学显微镜：形状和反射器
公开(公告)号：US20100210009A1
公开(公告)日：2010-08-19
申请号：US12769169
申请日：2010-04-28
申请人： Richard C. Willson , Paul Ruchhoeft
发明人： Richard C. Willson , Paul Ruchhoeft
IPC分类号： C12M1/34 , G01N21/01 , G01N33/48
CPC分类号： G01N33/54373 , B82Y5/00 , C12Q1/6837 , Y10S436/805 , Y10S977/701 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/773 , Y10S977/774 , Y10S977/834 , Y10S977/92 , C12Q2563/155
摘要： Labels and methods of producing labels for use in clinical, analytical and pharmaceutical development assays are provided. Labels may comprise shape-encoded particles which may be coupled to ligands such as DNA, RNA and antibodies, where different shapes are used to identify which ligand(s) are present. Labels may also comprise reflectors, including retroreflectors and retroreflectors susceptible to analyte-dependent assembly for efficient homogeneous assays.
摘要翻译：提供了生产用于临床，分析和药物开发测定的标签的标签和方法。标签可以包括可以与诸如DNA，RNA和抗体的配体偶联的形状编码的颗粒，其中使用不同的形状来鉴定哪些配体存在。标签还可以包括反射器，包括对分析物依赖性装配易于进行有效均匀测定的后向反射器和后向反射器。

34. 发明授权

US06623969B1 Process for testing catalysts using mass spectroscopy 有权
标题翻译：使用质谱法测试催化剂的方法
公开(公告)号：US06623969B1
公开(公告)日：2003-09-23
申请号：US09613082
申请日：2000-07-10
申请人： Richard C. Willson, III
发明人： Richard C. Willson, III
IPC分类号： G01N3110
CPC分类号： G01N31/10 , B01J19/0046 , B01J2219/00286 , B01J2219/00315 , B01J2219/00364 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00659 , B01J2219/00691 , B01J2219/00704 , B01J2219/00745 , B01J2219/00747 , C40B30/08 , C40B40/18 , Y10T436/214 , Y10T436/24
摘要： Methods for evaluating catalysts, in which a multicell holder, e.g., a honeycomb or plate, or a collection of individual support particles, is treated with solutions/suspensions of catalyst ingredients to produce cells, spots or pellets holding each of a variety of combinations of the ingredients, is dried, calcined or treated as necessary to stabilize the ingredients in the cells, spots or pellets, then is contacted with a potentially reactive feed stream or batch, e.g., biochemical, gas oil, hydrogen plus oxygen, propylene plus oxygen, CCl2F2 and hydrogen, etc. The reaction occurring in each cell can be measured, e.g., by infrared thermography, spectroscopic detection of products or residual reactants, or by sampling, e.g., multistreaming through low volume tubing, from the vicinity of each combination, followed by analysis, e.g., spectral analysis, chromatography, etc., or by observing temperature change in the vicinity of the catalyst, e.g., by thermographic techniques, to determine the relative efficacy of the catalysts in each combination. Robotic techniques can be employed in producing the cells, spots, pellets, etc.
摘要翻译：用于评价催化剂的方法，其中多单元保持器例如蜂窝或板或各个支撑颗粒的集合用催化剂成分的溶液/悬浮液处理以产生保持各种各样的组合的细胞，斑点或颗粒根据需要将成分干燥，煅烧或处理以稳定细胞，斑点或丸粒中的成分，然后与潜在的反应性进料流或批次接触，例如生化，瓦斯油，氢加氧，丙烯加氧， CCl 2 F 2和氢等。可以测量每个电池中发生的反应，例如通过红外热成像，产物或残余反应物的光谱检测，或通过采样，例如通过低体积管道从每个组合的附近进行多流式化，随后通过分析，例如光谱分析，色谱法等，或通过观察催化剂附近的温度变化，例如通过热成像技术测定 e催化剂在每种组合中的相对功效。机器人技术可用于生产细胞，斑点，丸粒等。

35. 发明授权

US4003770A Plasma spraying process for preparing polycrystalline solar cells 失效
标题翻译：制备多晶太阳能电池的等离子体喷涂工艺
公开(公告)号：US4003770A
公开(公告)日：1977-01-18
申请号：US561405
申请日：1975-03-24
申请人： Richard J. Janowiecki , Michael C. Willson , Douglas H. Harris
发明人： Richard J. Janowiecki , Michael C. Willson , Douglas H. Harris
IPC分类号： C23C4/04 , H01L21/00 , H01L31/0368 , H01L31/068 , H01L21/203 , H01L31/00
CPC分类号： H01L31/068 , C23C4/04 , H01L21/00 , H01L31/03682 , Y02E10/546 , Y02E10/547 , Y10S148/049 , Y10S148/122
摘要： Polycrystalline silicon films useful in preparing solar cells primarily for terrestrial application are prepared by a plasma spraying process. A doped silicon powder is injected into a high temperature ionized gas (plasma) to become molten and to be sprayed onto a low-cost substrate. Upon cooling, a dense polycrystalline silicon film is obtained. A p-n junction is formed on the sprayed film by spray deposition, diffusion or ion implantation. A sprayed junction is produced by plasma spraying a thin layer of silicon of opposite polarity or type over the initially deposited doped film. In forming a diffused junction, dopant is applied over the surface of the initial plasma-sprayed film usually from the vapor phase and heat is used to cause the dopant to diffuse into the film to form a shallow layer of opposite polarity to that in the original film. A junction is also formed by implanting dopant ions in the surface of the originally deposited film by the use of electrical fields. When used in conjunction with ohmic contacts and electrical conductors, the p-n junctions produced using plasma-sprayed polycrystalline silicon films are formed into solar cells which are useful for directly converting sunlight into electricity by means of the photovoltaic effect.
摘要翻译：用于制备主要用于陆地应用的太阳能电池的多晶硅膜通过等离子体喷涂方法制备。将掺杂的硅粉末注入到高温电离气体（等离子体）中以熔化并喷涂到低成本基材上。冷却后，得到致密的多晶硅膜。通过喷雾沉积，扩散或离子注入在喷涂膜上形成p-n结。通过在初始沉积的掺杂膜上等离子体喷涂相反极性或类型的薄层硅来产生喷涂结。在形成扩散结时，掺杂剂通常从气相中施加在初始等离子体喷涂膜的表面上，并且使用热使掺杂剂扩散到膜中以形成与原始反相极性相反的浅层电影。通过使用电场将掺杂剂离子注入原始沉积膜的表面中也形成结。当与欧姆接触和电导体结合使用时，使用等离子体喷涂的多晶硅膜产生的p-n结被形成为太阳能电池，其可用于通过光伏效应直接将阳光转换成电。

36. 发明授权

US12092641B2 Chemical detection assays 有权
公开(公告)号：US12092641B2
公开(公告)日：2024-09-17
申请号：US17271077
申请日：2019-08-22
申请人： Richard C. Willson , Victoria M. Hlavinka , Binh V. Vu , Federico Augusto Ruiz-Ruiz , Marco Antonio Rito Palomares , Mary A. Crum
发明人： Richard C. Willson , Victoria M. Hlavinka , Binh V. Vu , Federico Augusto Ruiz-Ruiz , Marco Antonio Rito Palomares , Mary A. Crum
IPC分类号： B01L3/00 , B01F23/00 , B01F23/41 , B01F101/23 , B23Q17/24 , C12M1/34 , C12Q1/04 , C12Q1/18 , C12Q1/32 , C12Q1/54 , C12Q1/686 , G01N21/3577 , G01N21/359 , G01N21/39 , G01N21/45 , G01N21/64 , G01N21/76 , G01N30/12 , G01N30/68 , G01N30/70 , G01N30/72 , G01N30/88 , G01N33/00 , G01N33/18 , G01N33/50 , G01N33/66 , G01N33/68 , G01N33/72
CPC分类号： G01N33/66 , C12Q1/32 , C12Q1/54 , G01N21/763 , G01N33/723
摘要： Disclosed are methods and kits for analyzing a sample comprising 1,5-anhydroglucitol and a possible first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte. Also provided are kits including reagents for practicing the claimed methods.

37. 发明授权

US09366506B2 Coated ballistic structures and methods of making same 有权
标题翻译：涂层弹道结构及其制作方法
公开(公告)号：US09366506B2
公开(公告)日：2016-06-14
申请号：US14031070
申请日：2013-09-19
申请人： Robert M. Willson , Michael C. Willson , Anil R. (Neil) Chaudhry
发明人： Robert M. Willson , Michael C. Willson , Anil R. (Neil) Chaudhry
IPC分类号： F41H5/02 , F41H5/04
CPC分类号： F41H5/0421 , F41H5/0414 , F41H5/0442 , F41H5/0492 , Y10T428/12535 , Y10T428/12576 , Y10T428/26
摘要： A durable ceramic and metallic coating has been applied to ceramic tiles to protect the tiles while undergoing a molten metal casting operation. The plasma sprayed coating consists of a ceramic top coat layer of aluminum oxide, zirconium oxide, or other oxides with or without a metallic bond coat layer and with or without a functionally gradient coating. This coating protects the underlying ceramic tile, which is composed of boron carbide, silicon carbide, alumina (Al2O3) or other type of hard ceramic, from reacting chemically with the molten metal. The molten metal is cast around the ceramic tiles to create a lattice of ceramic tiles that are used for protection from projectiles and shrapnel.
摘要翻译：耐用的陶瓷和金属涂层已被应用于瓷砖，以在进行熔融金属铸造操作时保护瓷砖。等离子喷涂涂层由氧化铝，氧化锆或具有或不具有金属粘结涂层的其它氧化物的陶瓷顶涂层组成，并具有或不具有功能梯度涂层。该涂层保护由碳化硼，碳化硅，氧化铝（Al 2 O 3）或其它类型的硬质陶瓷构成的下面的瓷砖与熔融金属化学反应。熔融金属铸造在瓷砖周围，以产生用于保护射弹和弹片的瓷砖格子。

38. 发明申请

US20130034498A1 OPTICAL MICROLABELS: SHAPES AND REFLECTORS 失效
标题翻译：光学显微镜：形状和反射器
公开(公告)号：US20130034498A1
公开(公告)日：2013-02-07
申请号：US13562260
申请日：2012-07-30
申请人： Richard C. Willson , Paul Ruchhoeft
发明人： Richard C. Willson , Paul Ruchhoeft
IPC分类号： G01N21/55 , G02B5/122 , A61K49/00
CPC分类号： G01N33/54373 , B82Y5/00 , C12Q1/6837 , Y10S436/805 , Y10S977/701 , Y10S977/702 , Y10S977/704 , Y10S977/705 , Y10S977/773 , Y10S977/774 , Y10S977/834 , Y10S977/92 , C12Q2563/155
摘要： Labels and methods of producing labels for use in clinical, analytical and pharmaceutical development assays are provided. Labels may comprise shape-encoded particles which may be coupled to ligands such as DNA, RNA and antibodies, where different shapes are used to identify which ligand(s) are present. Labels may also comprise reflectors, including retroreflectors and retroreflectors susceptible to analyte-dependent assembly for efficient homogeneous assays.
摘要翻译：提供了生产用于临床，分析和药物开发测定的标签的标签和方法。标签可以包括可以与诸如DNA，RNA和抗体的配体偶联的形状编码的颗粒，其中使用不同的形状来鉴定哪些配体存在。标签还可以包括反射器，包括对分析物依赖性装配易于进行有效均匀测定的后向反射器和后向反射器。

39. 发明授权

US07569347B2 Apparatus, methods and compositions for biotechnical separations 失效
标题翻译：用于生物技术分离的装置，方法和组合物
公开(公告)号：US07569347B2
公开(公告)日：2009-08-04
申请号：US11345252
申请日：2006-02-01
申请人： Richard C. Willson, III , Richard Don Goodin
发明人： Richard C. Willson, III , Richard Don Goodin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2521/107 , C12Q2527/125
摘要： RNA, preferably messenger RNA, is purified by use of selective precipitation, preferably by addition of compaction agents. Also included is a scalable method for the liquid-phase separation of DNA from RNA and RNA may also be recovered by fractional precipitation. Specific classes of compounds e.g. phase transfer catalysts (PTCs), most preferably selected polyamines of U.S. Pat. No. 6,617,108 polyamines which are quaternary compounds are unexpectedly potent in causing selective precipitation of DNA away from RNA, at low concentrations and in the presence of relatively elevated ionic strength selective removal of DNA can also remove both RNA and DNA, leaving behind a mixture which is advantageous for the further purification of, e.g., proteins. The invention include mini-preps, preferably of RNA or of plasmid and chromosomal DNA to obtain sequenceable and restriction digestible DNA in high yields in multiple simultaneous procedures and/or enhanced stripping of the compaction agent by a stripping method comprising high salt addition and pH shift, and combinations of these techniques. Isolation of RNA from bacterial lysates can employ selective precipitation by compaction agents.
摘要翻译：通过使用选择性沉淀，优选通过加入压实剂来纯化RNA，优选信使RNA。还包括用于DNA与RNA的液相分离的可扩展方法，RNA也可以通过分级沉淀来回收。特定类别的化合物例如相转移催化剂（PTC），最优选选择的多胺。作为季铵化合物的6,617,108多胺意外地以低浓度引起DNA远离RNA的选择性沉淀，并且在相对提升的离子强度的存在下，DNA的选择性去除也可以除去RNA和DNA，留下混合物对于例如蛋白质的进一步纯化是有利的。本发明包括优选的RNA或质粒和染色体DNA的微型预浸料，以在多个同时操作中获得高产率的可序列和限制性可消化的DNA，和/或通过包含高盐添加和pH漂移的剥离方法增强剥离压实剂，以及这些技术的组合。从细菌裂解液中分离RNA可以采用压实剂的选择性沉淀。

40. 发明申请

US20050064452A1 System and method for the detection of analytes 审中-公开
标题翻译：用于检测分析物的系统和方法
公开(公告)号：US20050064452A1
公开(公告)日：2005-03-24
申请号：US10832469
申请日：2004-04-26
申请人： Matthew Schmid , C. Willson
发明人： Matthew Schmid , C. Willson
IPC分类号： C12M1/34 , C12P19/34 , C12Q1/68 , G01N20060101
CPC分类号： C12Q1/6837 , C12Q2565/549 , C12Q2565/102
摘要： A system and method for the detection of an analyte using multiplexing of the sensing elements is described. In one embodiment, a sensor array includes sensing elements, and probes bound to one or more sensing elements. The sensor array is formed from a supporting member to which a plurality of sensing elements may be coupled. The sensing element may have a predefined shape, size or location. A signal may be produced when a target analyte interacts with a probe. In one embodiment, the identity of the target may be determined by the detection of the signals produced and the shapes of the sensing elements. Each analyte may be given a unique code that is represented by one or more sensing elements.
摘要翻译：描述了使用传感元件的多路复用来检测分析物的系统和方法。在一个实施例中，传感器阵列包括感测元件和绑定到一个或多个感测元件的探针。传感器阵列由多个感测元件可以联接到的支撑构件形成。感测元件可以具有预定的形状，尺寸或位置。当目标分析物与探针相互作用时，可能产生信号。在一个实施例中，可以通过检测所产生的信号和感测元件的形状来确定目标的身份。每个分析物可以被给予由一个或多个感测元件表示的唯一代码。

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