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11. 发明申请

US20130338041A1 NUCLEIC ACID ANALYSIS DEVICE AND NUCLEIC ACID ANALYZER 有权
标题翻译：核酸分析装置和核酸分析仪
公开(公告)号：US20130338041A1
公开(公告)日：2013-12-19
申请号：US14002125
申请日：2012-01-24
申请人： Koshin Hamasaki , Toshiro Saito , Takayuki Obara
发明人： Koshin Hamasaki , Toshiro Saito , Takayuki Obara
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6874 , C40B40/06 , G01N21/6452 , G01N21/648 , G01N2021/6439 , C12Q2521/543 , C12Q2535/122 , C12Q2563/149 , C12Q2565/513 , C12Q2523/308
摘要： In the conventional nucleic acid analysis devices and nucleic acid analyzers, there was no technique available for sequencing a single nucleic acid molecule easily and highly efficiently. The present invention enabled a highly efficient single molecule immobilization of nucleic acid with good reproductivity in a short time at a low price by providing small metallic bonding pads at predetermined positions on a support substrate, firmly fixing a hydrophobic linker on the bonding pads, and bonding on to the linker bulky microparticles onto which a single molecule of a nucleic acid sample fragment is immobilized. According to the present invention, in the nucleic acid analysis device which uses a nucleic acid analyzer, the nucleotide read length can be extended and many types of nucleic acid molecule to be analyzed can be analyzed at one time.
摘要翻译：在常规核酸分析装置和核酸分析仪中，没有技术可以容易且高效率地测序单个核酸分子。本发明通过在支撑衬底上的预定位置处提供小的金属粘合垫，在接合焊盘上牢固地固定疏水性接头，并且在接合焊盘上牢固地固定疏水性接头，从而能够以低价格在短时间内以高效率的单分子固定具有良好繁殖力的核酸在其上固定有单个分子的核酸样品片段的连接体庞大的微粒上。根据本发明，在使用核酸分析仪的核酸分析装置中，可以延长核苷酸读取长度，并且可以一次分析多种类型的要分析的核酸分子。

12. 发明申请

US20090245604A1 NUCLEIC ACID ANALYZING APPARATUS 有权
标题翻译：核酸分析装置
公开(公告)号：US20090245604A1
公开(公告)日：2009-10-01
申请号：US12369342
申请日：2009-02-11
申请人： Akira MAEKAWA , Toshiro Saito , Kiyoyuki Kagii , Takayuki Obara
发明人： Akira MAEKAWA , Toshiro Saito , Kiyoyuki Kagii , Takayuki Obara
IPC分类号： G06K9/00
CPC分类号： G01N21/6452 , G01N21/648 , G06T7/0004 , G06T7/136 , G06T2207/10064 , G06T2207/30024
摘要： The present invention provides a nucleic acid analyzing apparatus which achieves highly accurate analytical ability even in single molecule DNA analysis. The nucleic acid analyzing apparatus detects locations of fluorescent bright spots in image information about light emission, deletes defective bright spots, and thereby creates intensity trace data about proper bright spots.
摘要翻译：本发明提供即使在单分子DNA分析中也能够获得高精度的分析能力的核酸分析装置。核酸分析装置检测关于发光的图像信息中的荧光亮点的位置，删除有缺陷的亮点，从而生成关于适当的亮点的强度追踪数据。

13. 发明申请

US20060134667A1 Method for detecting fusion gene 审中-公开
标题翻译：融合基因检测方法
公开(公告)号：US20060134667A1
公开(公告)日：2006-06-22
申请号：US11274314
申请日：2005-11-16
申请人： Masatoshi Narahara , Toshiro Saito , Takayuki Obara , Kenji Yasuda
发明人： Masatoshi Narahara , Toshiro Saito , Takayuki Obara , Kenji Yasuda
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/6834 , C12Q1/6886 , C12Q2600/158 , C12Q2525/143 , C12Q2521/119 , C12Q2525/149
摘要： The present invention relates to a method for detecting fusion gene transcripts resulting from chromosomal translocation. Specifically, the method of the present invention comprises allowing at least two or more probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or complementary base sequence thereof, and each of which immobilized on a support, to hybridize with a sample containing a nucleic acid derived from a fusion gene, thereby allowing the detection of two or more fusion genes at a time.
摘要翻译：本发明涉及一种检测由染色体易位引起的融合基因转录物的方法。具体地说，本发明的方法包括允许至少两个或更多个探针，每个探针含有夹心融合基因或其互补碱基序列的断点的外显子的部分碱基序列，并且每个探针固定在载体上，与含有衍生自融合基因的核酸的样品杂交，从而一次检测两个或更多个融合基因。

14. 发明申请

US20130053280A1 NUCLEIC ACID ANALYSIS DEVICE, METHOD FOR PRODUCING SAME, AND NUCLEIC ACID ANALYZER 审中-公开
公开(公告)号：US20130053280A1
公开(公告)日：2013-02-28
申请号：US13697019
申请日：2011-05-09
申请人： Koshin Hamasaki , Toshiro Saito
发明人： Koshin Hamasaki , Toshiro Saito
IPC分类号： C40B60/12 , C40B40/10 , C40B50/14 , C40B40/06
CPC分类号： C12Q1/6806 , C12Q1/6834 , G01N21/05 , G01N21/554 , G01N21/648 , G01N33/54346 , C12Q2565/518 , C12Q2565/507 , C12Q2563/155
摘要： Disclosed is a technique for binding microparticles to patterned bonding pads of a metal (e.g., gold) formed on a support. The microparticles each carry a nucleic acid synthetase or DNA probe immobilized thereon for capturing a nucleic acid sample fragment. The technique involves forming, on a support surface, a film having a thickness equivalent to that of the bonding pads; controlling the size of microparticles with respect to the size of bonding pads; and thereby immobilizing microparticles each bearing a single nucleic acid sample fragment to the bonding pads in a one-to-one manner in a grid form. This allows high-density regular alignment and immobilization of many types of nucleic acid fragment samples on a support and enables high-throughput analysis of nucleic acid samples. Typically, immobilization of microparticles at 1-micrometer intervals easily provides a high density of 106 nucleic acid fragments per square millimeter.

15. 发明授权

US08228505B2 Apparatus and method for detecting target substance, or device used for these apparatus and method 有权
标题翻译：用于检测目标物质的装置和方法，或用于这些装置和方法的装置
公开(公告)号：US08228505B2
公开(公告)日：2012-07-24
申请号：US12233184
申请日：2008-09-18
申请人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
发明人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
IPC分类号： G01N21/55
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a material other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译：本发明的目的是检测具有高对比度的目标物质。本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率指数比底物。从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。检测由目标物质通过ev逝光的等离子体共振产生的光。根据本发明，可以减少对目标物质以外的材料的ev逝光的照射，从而可以减少来自目标物质以外的武器例如悬浮在目标物质周围的分子的发光。

16. 发明申请

US20120130050A1 BIOMOLECULE FIXING BOARD AND METHOD OF MANUFACTURING THE SAME 有权
标题翻译：生物分子固定板及其制造方法
公开(公告)号：US20120130050A1
公开(公告)日：2012-05-24
申请号：US13388671
申请日：2010-07-29
申请人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
发明人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
IPC分类号： C07K17/14 , C07K1/04
CPC分类号： G01N33/54353 , A61K2039/625 , G01N33/543 , G01N33/54393
摘要： This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.
摘要翻译：本发明提供一种生物分子修饰底物，其包含选择性地固定在其上给定区域上的生物分子。生物分子修饰基质包括：至少包含第一表面和第二表面的基底; 第一连接分子，其包含烃链和能够选择性地结合到烃链一端的第一表面的官能团，其通过该官能团与第一表面结合; 第二连接分子，其包含能够结合第一连接分子的烃链的反应性基团，其通过反应性基团和烃链之间的键与第一连接分子结合; 和通过第二连接分子与其结合的生物分子。

17. 发明申请

US20090023202A1 NUCLEIC ACID ANALYSIS DEVICE AND NUCLEIC ACID ANALYZER USING THE SAME 有权
标题翻译：使用相同的核酸分析装置和核酸分析仪
公开(公告)号：US20090023202A1
公开(公告)日：2009-01-22
申请号：US12173472
申请日：2008-07-15
申请人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
发明人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
IPC分类号： C12M1/34
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译：本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射进行局部表面等离子体激光，并且其中用于核酸分析的核酸探针或核酸合酶在样品中放置在产生表面等离子体的区域中。本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强效果的区域中固定DNA探针或核酸合酶，从而可以进行基本伸长反应的测量，而不用荧光分子去除未反应的底物。

18. 发明授权

US07022412B2 Member having metallic layer, its manufacturing method and its application 失效
标题翻译：具有金属层的构件，其制造方法及其应用
公开(公告)号：US07022412B2
公开(公告)日：2006-04-04
申请号：US10452291
申请日：2003-06-03
申请人： Hiroshi Yoshida , Toshiro Saito , Haruo Akahoshi
发明人： Hiroshi Yoshida , Toshiro Saito , Haruo Akahoshi
IPC分类号： B32B15/08 , B32B15/16 , H05K1/03 , H05K1/05
CPC分类号： B32B15/08 , C23C18/1605 , C23C18/1653 , C23C18/1696 , C23C18/2086 , C23C18/285 , C23C18/30 , H05K1/0346 , H05K1/036 , H05K3/181 , H05K3/184 , H05K3/38 , H05K3/387 , H05K3/4661 , H05K2203/0315 , H05K2203/1105 , Y10T428/24917 , Y10T428/31725 , Y10T428/31765
摘要： A member useful in a printed wiring board comprises a resin insulating layer, a metallic fine particle or catalyst layer, a metal oxide layer, and a metallic or electroless plating layer, wherein the resin insulating layer contains a resin containing an aromatic amide site such as an epoxy-terminated aromatic polyamide.
摘要翻译：可用于印刷线路板的部件包括树脂绝缘层，金属微粒或催化剂层，金属氧化物层和金属或化学镀层，其中树脂绝缘层包含含有芳族酰胺位点的树脂，例如环氧封端的芳族聚酰胺。

19. 发明授权

US4869659A Toggle-type die-fastening apparatus 失效
标题翻译：切割式模具紧固装置
公开(公告)号：US4869659A
公开(公告)日：1989-09-26
申请号：US211535
申请日：1988-05-25
申请人： Seiya Sakai , Mitsuo Suzuki , Toshiro Saito
发明人： Seiya Sakai , Mitsuo Suzuki , Toshiro Saito
IPC分类号： B29C45/76
CPC分类号： B29C45/7653
摘要： A toggle-type die-fastening apparatus has a movable platen that carries one mold part and is supported by and guided along a plurality of tie bars whose one ends are fixed to a fixed platen on which another mold part is mounted. The other ends of the tie bars are supported by a toggle support, and a toggle mechanism is disposed between the toggle support and the movable platen for pressing the mold part carried by the movable platen against the mold part mounted on the fixed platen. A die-fastening force adjusting nut is threaded onto the end portion of each tie bar that is closer to the toggle support so as to bear the reaction of the die-fastening force generated by the toggle mechanism. An adjusting cylinder is formed in this portion, and it is connected to a hydraulic circuit so that the pressure within each of the cylinders is controlled by a pressure adjusting valve in such a manner as to correspond to a die-fastening force set by a setting device. Therefore, the die-fastening forces of all the tie bars can be made the same, thereby eliminating any imbalance between the die-fastening forces of the tie bars caused by changes in thermal balance during the operation of the molding machine.

20. 发明授权

US07734422B2 Method for evaluating DNA probes position on substrate 失效
标题翻译：评估DNA探针在底物上的位置的方法
公开(公告)号：US07734422B2
公开(公告)日：2010-06-08
申请号：US11113195
申请日：2005-04-25
申请人： Kazuhito Rokutan , Hiroyuki Tomita , Toshiro Saito
发明人： Kazuhito Rokutan , Hiroyuki Tomita , Toshiro Saito
IPC分类号： G01N33/48 , G06F19/00 , G01N31/00 , C07H21/00 , C07H21/04 , C12Q1/68 , C12P19/34 , C12M1/00
CPC分类号： G06F19/20 , C12Q1/6883 , C12Q2565/513 , C12Q2600/158 , C12Q2600/166
摘要： An oligonucleotide array comprising an array of multiple oligonucleotides with different base sequences fixed onto known and separate positions on a support substrate, wherein said oligonucleotides are biological stress related genes or complementary sequence chains to the said genes, and the said oligonucleotides are classified according to their gene functions, wherein the fixation region on the support substrate is divided into the said classification.
摘要翻译：一种寡核苷酸阵列，其包含具有不同碱基序列的多个寡核苷酸阵列，其固定在载体底物上的已知和分开的位置上，其中所述寡核苷酸是与所述基因相关的生物应激相关基因或互补序列链，并且所述寡核苷酸根据它们基因功能，其中支撑衬底上的固定区域被划分为所述分类。

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