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1. 发明申请

US20120130050A1 BIOMOLECULE FIXING BOARD AND METHOD OF MANUFACTURING THE SAME 有权
标题翻译：生物分子固定板及其制造方法
公开(公告)号：US20120130050A1
公开(公告)日：2012-05-24
申请号：US13388671
申请日：2010-07-29
申请人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
发明人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
IPC分类号： C07K17/14 , C07K1/04
CPC分类号： G01N33/54353 , A61K2039/625 , G01N33/543 , G01N33/54393
摘要： This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.
摘要翻译：本发明提供一种生物分子修饰底物，其包含选择性地固定在其上给定区域上的生物分子。生物分子修饰基质包括：至少包含第一表面和第二表面的基底; 第一连接分子，其包含烃链和能够选择性地结合到烃链一端的第一表面的官能团，其通过该官能团与第一表面结合; 第二连接分子，其包含能够结合第一连接分子的烃链的反应性基团，其通过反应性基团和烃链之间的键与第一连接分子结合; 和通过第二连接分子与其结合的生物分子。

2. 发明授权

US09040251B2 Biomolecule fixing board and method of manufacturing the same 有权
标题翻译：生物分子固定板及其制造方法
公开(公告)号：US09040251B2
公开(公告)日：2015-05-26
申请号：US13388671
申请日：2010-07-29
申请人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
发明人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
IPC分类号： G01N33/53 , C07K1/04 , G01N33/551 , G01N33/543 , A61K39/00
CPC分类号： G01N33/54353 , A61K2039/625 , G01N33/543 , G01N33/54393
摘要： This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.
摘要翻译：本发明提供一种生物分子修饰底物，其包含选择性地固定在其上给定区域上的生物分子。生物分子修饰基质包括：至少包含第一表面和第二表面的基底; 第一连接分子，其包含烃链和能够选择性地结合到烃链一端的第一表面的官能团，其通过该官能团与第一表面结合; 第二连接分子，其包含能够结合第一连接分子的烃链的反应性基团，其通过反应性基团和烃链之间的键与第一连接分子结合; 和通过第二连接分子与其结合的生物分子。

3. 发明授权

US08228505B2 Apparatus and method for detecting target substance, or device used for these apparatus and method 有权
标题翻译：用于检测目标物质的装置和方法，或用于这些装置和方法的装置
公开(公告)号：US08228505B2
公开(公告)日：2012-07-24
申请号：US12233184
申请日：2008-09-18
申请人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
发明人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
IPC分类号： G01N21/55
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a material other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译：本发明的目的是检测具有高对比度的目标物质。本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率指数比底物。从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。检测由目标物质通过ev逝光的等离子体共振产生的光。根据本发明，可以减少对目标物质以外的材料的ev逝光的照射，从而可以减少来自目标物质以外的武器例如悬浮在目标物质周围的分子的发光。

4. 发明申请

US20090023202A1 NUCLEIC ACID ANALYSIS DEVICE AND NUCLEIC ACID ANALYZER USING THE SAME 有权
标题翻译：使用相同的核酸分析装置和核酸分析仪
公开(公告)号：US20090023202A1
公开(公告)日：2009-01-22
申请号：US12173472
申请日：2008-07-15
申请人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
发明人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
IPC分类号： C12M1/34
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译：本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射进行局部表面等离子体激光，并且其中用于核酸分析的核酸探针或核酸合酶在样品中放置在产生表面等离子体的区域中。本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强效果的区域中固定DNA探针或核酸合酶，从而可以进行基本伸长反应的测量，而不用荧光分子去除未反应的底物。

5. 发明授权

US09207235B2 Nucleic acid analyzer, reaction device for nucleic acid analysis and substrate of reaction device for nucleic acid analysis 有权
标题翻译：核酸分析仪，核酸分析反应装置和核酸分析反应装置底物
公开(公告)号：US09207235B2
公开(公告)日：2015-12-08
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
IPC分类号： B01L3/00 , G01N33/543 , G01N21/64 , G01N33/53 , G01N33/58 , G01N35/00
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译：提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。

6. 发明授权

US08865403B2 Nucleic acid analyzing device and nucleic acid analyzer 有权
标题翻译：核酸分析装置和核酸分析仪
公开(公告)号：US08865403B2
公开(公告)日：2014-10-21
申请号：US12997469
申请日：2009-05-13
申请人： Masatoshi Narahara , Toshiro Saito , Naoshi Itabashi , Jiro Yamamoto , Hiroyuki Uchiyama
发明人： Masatoshi Narahara , Toshiro Saito , Naoshi Itabashi , Jiro Yamamoto , Hiroyuki Uchiyama
IPC分类号： C12Q1/68 , C12M1/36 , G01N21/00 , G01N21/64
CPC分类号： G01N21/648
摘要： An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.
摘要翻译：本发明的目的在于从未反应的底物的荧光团区分与通过核酸合成并入探针中的核苷酸连接的单个荧光团。本发明涉及通过荧光分析样品中的核酸的核酸分析装置，其中通过照射产生局部表面等离子体激元，并且用于分析样品中的核酸的探针位于表面等离子体为生成。根据本发明，由于可以有效地产生由于表面等离子体激元引起的荧光增强作用并且将探针固定在荧光增强作用范围内的区域，因此可以测量核酸合成而不去除未反应的附着有荧光团的核苷酸。

7. 发明申请

US20120316087A1 Nucleic Acid Analyzer, Reaction Device for Nucleic Acid Analysis and Substrate of Reaction Device for Nucleic Acid Analysis 有权
标题翻译：核酸分析仪，用于核酸分析的反应装置和用于核酸分析的反应装置的底物
公开(公告)号：US20120316087A1
公开(公告)日：2012-12-13
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
IPC分类号： C40B60/10
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译：提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。

8. 发明申请

US20090079988A1 APPARATUS AND METHOD FOR DETECTING TARGET SUBSTANCE, OR DEVICE USED FOR THESE APPARATUS AND METHOD 有权
标题翻译：用于检测目标物质的装置和方法或用于这些装置和方法的装置
公开(公告)号：US20090079988A1
公开(公告)日：2009-03-26
申请号：US12233184
申请日：2008-09-18
申请人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
发明人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
IPC分类号： G01N21/55 , G01J1/58
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译：本发明的目的是检测具有高对比度的目标物质。本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率指数比底物。从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。检测由目标物质通过ev逝光的等离子体共振产生的光。根据本发明，可以减少对目标物质以外的其他武器的ev逝光的照射，从而可以减少来自与目标物质以外的武装物（例如，悬浮在目标物质周围的分子）的发光。

9. 发明授权

US08865459B2 Nucleic acid analysis device and nucleic acid analyzer using the same 有权
标题翻译：核酸分析装置和使用其的核酸分析仪
公开(公告)号：US08865459B2
公开(公告)日：2014-10-21
申请号：US12173472
申请日：2008-07-15
申请人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
发明人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
IPC分类号： B01L3/00 , G01N21/00 , G01N21/64 , G01N21/05
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译：本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射进行局部表面等离子体激光，并且其中用于核酸分析的核酸探针或核酸合酶在样品中放置在产生表面等离子体的区域中。本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强效果的区域中固定DNA探针或核酸合酶，从而可以进行基本伸长反应的测量，而不用荧光分子去除未反应的底物。

10. 发明申请

US20110081655A1 NUCLEIC ACID ANALYZING DEVICE AND NUCLEIC ACID ANALYZER 有权
标题翻译：核酸分析装置和核酸分析仪
公开(公告)号：US20110081655A1
公开(公告)日：2011-04-07
申请号：US12997469
申请日：2009-05-13
申请人： Masatoshi Narahara , Toshiro Saito , Naoshi Itabashi , Jiro Yamamoto , Hiroyuki Uchiyama
发明人： Masatoshi Narahara , Toshiro Saito , Naoshi Itabashi , Jiro Yamamoto , Hiroyuki Uchiyama
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N21/648
摘要： An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.
摘要翻译：本发明的目的在于从未反应的底物的荧光团区分与通过核酸合成并入探针中的核苷酸连接的单个荧光团。本发明涉及通过荧光分析样品中的核酸的核酸分析装置，其中通过照射产生局部表面等离子体激元，并且用于分析样品中的核酸的探针位于表面等离子体为生成。根据本发明，由于可以有效地产生由于表面等离子体激元引起的荧光增强作用并且将探针固定在荧光增强作用范围内的区域，因此可以测量核酸合成而不去除未反应的附着有荧光团的核苷酸。

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