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1. 发明授权

US07734422B2 Method for evaluating DNA probes position on substrate 失效
标题翻译：评估DNA探针在底物上的位置的方法
公开(公告)号：US07734422B2
公开(公告)日：2010-06-08
申请号：US11113195
申请日：2005-04-25
申请人： Kazuhito Rokutan , Hiroyuki Tomita , Toshiro Saito
发明人： Kazuhito Rokutan , Hiroyuki Tomita , Toshiro Saito
IPC分类号： G01N33/48 , G06F19/00 , G01N31/00 , C07H21/00 , C07H21/04 , C12Q1/68 , C12P19/34 , C12M1/00
CPC分类号： G06F19/20 , C12Q1/6883 , C12Q2565/513 , C12Q2600/158 , C12Q2600/166
摘要： An oligonucleotide array comprising an array of multiple oligonucleotides with different base sequences fixed onto known and separate positions on a support substrate, wherein said oligonucleotides are biological stress related genes or complementary sequence chains to the said genes, and the said oligonucleotides are classified according to their gene functions, wherein the fixation region on the support substrate is divided into the said classification.
摘要翻译：一种寡核苷酸阵列，其包含具有不同碱基序列的多个寡核苷酸阵列，其固定在载体底物上的已知和分开的位置上，其中所述寡核苷酸是与所述基因相关的生物应激相关基因或互补序列链，并且所述寡核苷酸根据它们基因功能，其中支撑衬底上的固定区域被划分为所述分类。

2. 发明申请

US20050208561A1 Method for evaluating DNA probes position on substrate 失效
标题翻译：评估DNA探针在底物上的位置的方法
公开(公告)号：US20050208561A1
公开(公告)日：2005-09-22
申请号：US11113195
申请日：2005-04-25
申请人： Kazuhito Rokutan , Hiroyuki Tomita , Toshiro Saito
发明人： Kazuhito Rokutan , Hiroyuki Tomita , Toshiro Saito
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N37/00 , C12M1/34
CPC分类号： G06F19/20 , C12Q1/6883 , C12Q2565/513 , C12Q2600/158 , C12Q2600/166
摘要： An oligonucleotide array comprising an array of multiple oligonucleotides with different base sequences fixed onto known and separate positions on a support substrate, wherein said oligonucleotides are biological stress related genes or complementary sequence chains to the said genes, and the said oligonucleotides are classified according to their gene functions, wherein the fixation region on the support substrate is divided into the said classification.
摘要翻译：一种寡核苷酸阵列，其包含具有不同碱基序列的多个寡核苷酸阵列，其固定在载体底物上的已知和分开的位置上，其中所述寡核苷酸是与所述基因相关的生物应激相关基因或互补序列链，并且所述寡核苷酸根据它们基因功能，其中支撑衬底上的固定区域被划分为所述分类。

3. 发明申请

US20080281531A1 Method for Diagnosing Depression 审中-公开
标题翻译：诊断抑郁症的方法
公开(公告)号：US20080281531A1
公开(公告)日：2008-11-13
申请号：US12048241
申请日：2008-03-14
申请人： Kazuhito Rokutan , Tetsuro Ohmori , Toshiro Saito , Masayuki Ohta
发明人： Kazuhito Rokutan , Tetsuro Ohmori , Toshiro Saito , Masayuki Ohta
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6883 , C12Q2600/106 , C12Q2600/158 , Y02A90/24 , Y02A90/26
摘要： This invention relates to a method for diagnosing whether or not a subject suffers from depression in a simple manner with high accuracy using the peripheral whole blood sample of the subject. Specifically, the present invention relates to a method for diagnosing depression comprising the steps of: measuring expression levels of 18 genes selected from the group consisting of FASLG; CX3CR1, TBX21, ID2, SLAMF7, PRSS23, YWHAQ, TARDBP, ADRB2, PPP1R8, MMAA, SQLE, PDHA1, HAVCR2, RACGAP1, AHNAK, EDG8, and DUSP5, in peripheral blood isolated from a subject; and determining whether or not the subject suffers from depression based on the expression levels of the 18 genes.
摘要翻译：本发明涉及使用对象的周边全血样品以高精度诊断受试者是否患有抑郁症的方法。具体地，本发明涉及一种诊断抑郁症的方法，包括以下步骤：测量选自FASLG的18种基因的表达水平; CX3CR1，TBX21，ID2，SLAMF7，PRSS23，YWHAQ，TARDBP，ADRB2，PPP1R8，MMAA，SQLE，PDHA1，HAVCR2，RACGAP1，AHNAK，EDG8和DUSP5; 并根据18个基因的表达水平确定受试者是否患有抑郁症。

4. 发明申请

US20050282207A1 Method of stress evaluation 审中-公开
标题翻译：压力评估方法
公开(公告)号：US20050282207A1
公开(公告)日：2005-12-22
申请号：US11152365
申请日：2005-06-15
申请人： Kazuhito Rokutan , Kyoko Morita , Kumiko Kamihara , Toshiro Saito
发明人： Kazuhito Rokutan , Kyoko Morita , Kumiko Kamihara , Toshiro Saito
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q2600/158
摘要： This invention relates to a simple method for evaluating stress of a normal healthy subject with high accuracy is provided. In this method, the expression profile of specific genes that serve as stress marker genes is analyzed, and stress of the subject is evaluated based on the analysis results.
摘要翻译：本发明涉及一种用于以高精度评估正常健康受试者的应力的简单方法。在该方法中，分析了作为应激标记基因的特定基因的表达谱，并根据分析结果评价受试者的应激。

5. 发明申请

US20050239110A1 Method of diagnosing depression 审中-公开
标题翻译：诊断抑郁症的方法
公开(公告)号：US20050239110A1
公开(公告)日：2005-10-27
申请号：US11091674
申请日：2005-03-29
申请人： Kazuhito Rokutan , Tetsuro Ohmori , Kyoko Morita , Masayuki Ohta , Toshiro Saito
发明人： Kazuhito Rokutan , Tetsuro Ohmori , Kyoko Morita , Masayuki Ohta , Toshiro Saito
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N37/00
CPC分类号： C12Q1/6883 , C12Q2600/112 , C12Q2600/158 , Y02A90/24 , Y02A90/26
摘要： This invention provides a novel method of diagnosing the conditions of depression of a patient in a simple, objective, and accurate manner. In this method, gene expression is analyzed using mRNA of a subject's peripheral blood to evaluate whether or not the subject is afflicted with depression, the type of depression of a subject who had been evaluated as being afflicted with depression is identified, and the conditions of depression are then diagnosed in accordance with the type of depression.
摘要翻译：本发明提供一种以简单，客观，准确的方式诊断患者抑郁状况的新方法。在该方法中，使用受试者的外周血的mRNA来分析基因表达，以评价受试者是否患有抑郁症，鉴定被评估为患有抑郁症的受试者的抑郁症的类型，以及然后根据抑郁症的类型诊断抑郁症。

6. 发明申请

US20130053280A1 NUCLEIC ACID ANALYSIS DEVICE, METHOD FOR PRODUCING SAME, AND NUCLEIC ACID ANALYZER 审中-公开
公开(公告)号：US20130053280A1
公开(公告)日：2013-02-28
申请号：US13697019
申请日：2011-05-09
申请人： Koshin Hamasaki , Toshiro Saito
发明人： Koshin Hamasaki , Toshiro Saito
IPC分类号： C40B60/12 , C40B40/10 , C40B50/14 , C40B40/06
CPC分类号： C12Q1/6806 , C12Q1/6834 , G01N21/05 , G01N21/554 , G01N21/648 , G01N33/54346 , C12Q2565/518 , C12Q2565/507 , C12Q2563/155
摘要： Disclosed is a technique for binding microparticles to patterned bonding pads of a metal (e.g., gold) formed on a support. The microparticles each carry a nucleic acid synthetase or DNA probe immobilized thereon for capturing a nucleic acid sample fragment. The technique involves forming, on a support surface, a film having a thickness equivalent to that of the bonding pads; controlling the size of microparticles with respect to the size of bonding pads; and thereby immobilizing microparticles each bearing a single nucleic acid sample fragment to the bonding pads in a one-to-one manner in a grid form. This allows high-density regular alignment and immobilization of many types of nucleic acid fragment samples on a support and enables high-throughput analysis of nucleic acid samples. Typically, immobilization of microparticles at 1-micrometer intervals easily provides a high density of 106 nucleic acid fragments per square millimeter.

7. 发明授权

US08228505B2 Apparatus and method for detecting target substance, or device used for these apparatus and method 有权
标题翻译：用于检测目标物质的装置和方法，或用于这些装置和方法的装置
公开(公告)号：US08228505B2
公开(公告)日：2012-07-24
申请号：US12233184
申请日：2008-09-18
申请人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
发明人： Masatoshi Narahara , Satoshi Takahashi , Toshiro Saito
IPC分类号： G01N21/55
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a material other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译：本发明的目的是检测具有高对比度的目标物质。本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率指数比底物。从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。检测由目标物质通过ev逝光的等离子体共振产生的光。根据本发明，可以减少对目标物质以外的材料的ev逝光的照射，从而可以减少来自目标物质以外的武器例如悬浮在目标物质周围的分子的发光。

8. 发明申请

US20120130050A1 BIOMOLECULE FIXING BOARD AND METHOD OF MANUFACTURING THE SAME 有权
标题翻译：生物分子固定板及其制造方法
公开(公告)号：US20120130050A1
公开(公告)日：2012-05-24
申请号：US13388671
申请日：2010-07-29
申请人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
发明人： Yasuhiko Tada , Hiroshi Yoshida , Toshiro Saito , Masatoshi Narahara , Hiroaki Nakagawa
IPC分类号： C07K17/14 , C07K1/04
CPC分类号： G01N33/54353 , A61K2039/625 , G01N33/543 , G01N33/54393
摘要： This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.
摘要翻译：本发明提供一种生物分子修饰底物，其包含选择性地固定在其上给定区域上的生物分子。生物分子修饰基质包括：至少包含第一表面和第二表面的基底; 第一连接分子，其包含烃链和能够选择性地结合到烃链一端的第一表面的官能团，其通过该官能团与第一表面结合; 第二连接分子，其包含能够结合第一连接分子的烃链的反应性基团，其通过反应性基团和烃链之间的键与第一连接分子结合; 和通过第二连接分子与其结合的生物分子。

9. 发明申请

US20110281320A1 DEVICE FOR ANALYZING NUCLEIC ACIDS AND APPARATUS FOR ANALYZING NUCLEIC ACIDS 审中-公开
标题翻译：用于分析核酸的装置和分析核酸的装置
公开(公告)号：US20110281320A1
公开(公告)日：2011-11-17
申请号：US13147117
申请日：2010-01-18
申请人： Toshiro Saito , Kazumichi Imai
发明人： Toshiro Saito , Kazumichi Imai
IPC分类号： C12N11/14 , C12M1/34 , C07H21/00 , C12M1/40 , C12N11/00
CPC分类号： C12Q1/6816 , C12Q2565/50 , C12Q2563/143
摘要： An object of the present invention is to regularly align microparticles, on each of which a nucleic acid synthetase or a DNA probe capable of capturing a nucleic acid sample fragment is immobilized, on a support so as to improve throughput of nucleic acid analysis. The present invention relates to a method comprising immobilizing a nucleic acid synthetase, a DNA probe, or the like in advance to a microparticle, forming a pattern of metal pads each having a diameter smaller than the microparticle diameter with gold or the like on a support, and allowing a microparticle to be bound to the pads via a chemical bond. In addition, when the surfaces of microparticles are electrically charged, a pattern of metal pads each having a diameter equivalent to or larger than the microparticle diameter is formed with gold or the like on a support and a microparticle is allowed to be bound to the pads via a chemical bond. According to the present invention, many types of nucleic acid fragment samples can be regularly aligned at a high density and immobilized on a support. This allows high throughput analysis of nucleic acid samples. For example, if microparticles are immobilized at 1-micron pitches, a high density of 106 nucleic acid fragments/emm2 can be readily achieved.
摘要翻译：本发明的一个目的是在载体上规则地将微粒（其中每一个核酸合成酶或能够捕获核酸样品片段的DNA探针）定位在载体上，以便提高核酸分析的生产能力。本发明涉及一种将核酸合成酶，DNA探针等预先固定在微粒上的方法，在支撑体上形成具有小于微粒径的直径的金属垫的图案，并且允许微粒通过化学键与焊盘结合。此外，当微粒表面带电时，在支撑体上形成金等等于或大于微粒直径的金属垫的图案，并且允许微粒结合到垫通过化学键。根据本发明，许多类型的核酸片段样品可以高密度地定期排列并固定在载体上。这允许核酸样品的高通量分析。例如，如果微粒以1微米间距固定，则可以容易地实现高密度的106个核酸片段/ emm2。

10. 发明申请

US20090023202A1 NUCLEIC ACID ANALYSIS DEVICE AND NUCLEIC ACID ANALYZER USING THE SAME 有权
标题翻译：使用相同的核酸分析装置和核酸分析仪
公开(公告)号：US20090023202A1
公开(公告)日：2009-01-22
申请号：US12173472
申请日：2008-07-15
申请人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
发明人： Masatoshi Narahara , Toshiro Saito , Satoshi Takahashi
IPC分类号： C12M1/34
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译：本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射进行局部表面等离子体激光，并且其中用于核酸分析的核酸探针或核酸合酶在样品中放置在产生表面等离子体的区域中。本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强效果的区域中固定DNA探针或核酸合酶，从而可以进行基本伸长反应的测量，而不用荧光分子去除未反应的底物。

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