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    • 3. 发明授权
    • 박테리아셀룰로오즈의제조방법
    • KR100438394B1
    • 2004-08-16
    • KR1019970703540
    • 1996-08-30
    • 아지노모토 가부시키가이샤
    • 코우다토루나리토미타카아키야노히사토요시나가푸미히로
    • C12P19/04
    • C12P19/04Y10S435/822Y10S435/823
    • The present invention relates to a process for the production of cellulosic material which comprises culturing a cellulose-producing bacterium while maintaining the internal pressure within the fermentation tank at about 1.1 kg/cm A or more, preferably at about 1.2 kg/cm A or more, more preferably at about 1.5 kg/cm A or more, generally in the later stage of the cultivation (the growth decline phase and stationary phase), namely, at the stage where a concentration of the cellulosic material in a culture medium reaches about 10 g/L or more, preferably about 12 g/L or more; at the culturing stage where an apparent density of the culture medium at 10 rad/s or 1 l/s reaches about 10 Pa.s or more; at the culturing stage where the K value (consistency index) reaches about 10 Pa.s or more considering that rheology follows the Power law model; or at the stage where the oxygen-demand of the culture medium reaches about 35 mmol/L.hr or more. According to the present invention, the power required for agitation during the cultivation for the production of BC may be remarkably reduced, and the BC production rate and yield may be increased.
    • 生产纤维素材料的方法本发明涉及生产纤维素材料的方法,其包括培养产纤维素细菌,同时保持发酵罐内的内部压力为约1.1kg / cm 2。 A或更多,优选约1.2kg / cm 2 A或更多,更优选约1.5kg / cm 2 通常在培养的后期阶段(生长衰退期和静止期),即在培养基中纤维素材料的浓度达到约10g / L或更高,优选约12 g / L以上; 在10rad / s或1l / s的培养基表观密度达到约10Pa.s或更高的培养阶段; 在K值(稠度指数)达到约10Pa·s的培养阶段, 或更多考虑流变学遵循幂律模型; 或者在培养基的需氧量达到约35mmol / L.hr或更高的阶段。 根据本发明,用于生产BC的培养期间搅拌所需的动力可显着降低,并且BC生产速率和产量可增加。 <图像>
    • 5. 发明公开
    • 만노스의 정량용 시약
    • 量化分析方法和定量分析方法
    • KR1020010051468A
    • 2001-06-25
    • KR1020000065556
    • 2000-11-06
    • 세키스이 메디칼 가부시키가이샤
    • 에비누마히로유키우시자와고지
    • C12Q1/32
    • C12Q1/32Y10S435/823Y10S435/962
    • PURPOSE: Provided a method for quantitatively determining mannose, which comprises reacting mannose in a specimen with an enzyme oxidizing the mannose by dehydrogenation, in the presence of an electron acceptor, and quantitatively determining a reduced form of the electron acceptor. CONSTITUTION: The method quantitatively analyzing the mannose comprises reacting mannose in a specimen with an enzyme which is capable of oxidizing the mannose by dehydrogenation, in the presence of an electron acceptor, and quantitatively determining a formed reductant of the electron acceptor. Wherein, the enzyme is preferably a glucose dehydrogenase which belongs to an enzyme number EC class 1.1.1.119, more preferably a aldohexose dehydrogenase which is derived from a microorganism belonging to a gluconobacter genus. As the specimen, it is preferred that the specimen is at least one biological specimen selected from the group consisting of blood, serum, plasma, cerebrospinal fluid and urine, or a specimen prepared from biological specimen.
    • 目的:提供一种定量测定甘露糖的方法,其包括使样品中的甘露糖与通过脱氢氧化甘露糖的酶在电子受体存在下反应,并定量测定电子受体的还原形式。 构成:定量分析甘露糖的方法包括使样品中的甘露糖与能够通过脱氢氧化甘露糖的酶在电子受体存在下反应,并定量测定形成的电子受体的还原剂。 其中,酶优选为属于酶类型EC类别1.1.1.119的葡萄糖脱氢酶,更优选衍生自属于葡萄球菌属的微生物的己糖异构酶。 作为标本,优选为从血液,血清,血浆,脑脊髓液和尿液中选出的至少一种生物样品,或者由生物样品制备的试样。