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    • 2. 发明公开
    • 캔디다 유틸리스 기원의 글루타티온 신테타제 암호화 유전자
    • 从坎迪达酵母编码GLUTATHIONE SYNTHETASE的基因
    • KR1020050027009A
    • 2005-03-17
    • KR1020040070005
    • 2004-09-02
    • 아지노모토 가부시키가이샤
    • 니시우치히로아키니시무라야스시
    • C12N15/55
    • C12N9/93A23L27/21A23V2002/00C12P13/12C12P21/02C12Y603/02003A23V2250/0616
    • A gene encoding glutathione synthetase from Candida utilis is provided, thereby cheaply producing yeast extract using Candida utilis which is grown by using the glutathione synthetase and gamma-glutamylcysteine synthetase genes, does not produce ethanol as a by-product under strictly aerobic condition, and has high content of gamma-glutamylcysteine. A DNA encoding a protein having (A) the amino acid sequence of SEQ ID NO: 2; or (B) the amino acid sequence of SEQ ID NO: 2 including substitution, deletion, insertion or addition of one or more amino acids, and glutathione synthetase activity is provided, wherein the DNA (a) comprises 58th to 1485th nucleotides in the nucleotide sequence of SEQ ID NO: 1; or (b) is hybridizable with the DNA comprising the 58th to 1485th nucleotides in the nucleotide sequence of SEQ ID NO: 1 or a probe that can be prepared therefrom under stringent conditions, and encodes a protein having glutathione synthetase activity. A microorganism Candida utilis which is modified by the DNA to have glutathione synthetase activity of 0.003 micromol GSH/mg protein per hour or less is provided.
    • 提供了从产朊假丝酵母中编码谷胱甘肽合成酶的基因,由此通过使用谷胱甘肽合成酶和γ-谷氨酰半胱氨酸合成酶基因生长的产朊假单胞菌廉价生产酵母提取物,在严格有氧条件下不产生作为副产物的乙醇,并具有 高含量的γ-谷氨酰半胱氨酸。 编码具有(A)SEQ ID NO:2的氨基酸序列的蛋白质的DNA; 或(B)包括一个或多个氨基酸的取代,缺失,插入或添加的SEQ ID NO:2的氨基酸序列,并且提供谷胱甘肽合成酶活性,其中DNA(a)在核苷酸中包含58至1485位核苷酸 SEQ ID NO:1的序列; 或(b)可与SEQ ID NO:1的核苷酸序列中包含第58至第1485位核苷酸的DNA杂交,或在严格条件下可由其制备的探针,并编码具有谷胱甘肽合成酶活性的蛋白质。 提供了由DNA修饰为每小时或更少具有0.003微摩尔GSH / mg蛋白质的谷胱甘肽合成酶活性的微生物产朊假丝酵母。
    • 9. 发明公开
    • 훈연 향미가 강화된 식품
    • 通过将含有硫的化合物加入到食品中的具有增强烟熏味的食物
    • KR1020050014757A
    • 2005-02-07
    • KR1020040060379
    • 2004-07-30
    • 아지노모토 가부시키가이샤
    • 니시모리유키니시우치히로아키이토유지니시무라야스시가와구치히로카즈
    • A23L1/318
    • A23L13/70A23L27/00A23L27/27A23V2200/15
    • PURPOSE: Food with enhanced smoke flavor is provided, thereby reinforcing the smoke flavor in food by adding sulfur-containing compound to food, so that treatment of food with smoking or dipping food in smoking liquid can be eliminated. CONSTITUTION: The food with enhanced smoke flavor is prepared by adding sulfur-containing compound into food selected from processed meat product, processed seafood product and vegetables, wherein the sulfur-containing compound is selected from sulfur-containing amino acid, sulfur-containing peptide and sulfur-containing vitamin; the sulfur-containing compound is selected from cysteine, cystine, methionine, taurine, glutathione, glutamylcysteine, cysteinyl glycine, thiamine and salts thereof; and the amount of sulfur-containing compound added is 0.000000001 to 10.0%.
    • 目的:提供具有增强烟味的食品,通过向食品中加入含硫化合物来增强食品中的烟味,从而可以消除吸烟或吸食食物吸食食物的处理。 构成:通过向选自加工肉制品,加工海产品和蔬菜的食品中加入含硫化合物制备具有增强烟味的食品,其中含硫化合物选自含硫氨基酸,含硫肽和 含硫维生素; 含硫化合物选自半胱氨酸,胱氨酸,甲硫氨酸,牛磺酸,谷胱甘肽,谷氨酰半胱氨酸,半胱氨酰甘氨酸,硫胺素及其盐; 加入的含硫化合物的量为0.00000000〜10.0%。