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    • 1. 发明公开
    • 균류를 이용한 키토산 생산방법
    • 从真菌生产壳聚糖的方法
    • KR1020030071389A
    • 2003-09-03
    • KR1020020011063
    • 2002-02-28
    • 강수태공청식경상대학교산학협력단
    • 강수태공청식오광수
    • C12P19/04
    • PURPOSE: A method for producing chitosan from fungi is provided, thereby simplifying the production process of chitosan, and decreasing the amount of waste water and waste matters. CONSTITUTION: A method for producing chitosan from fungi comprises the steps of: culturing Absidia coerulea IFO 5301 or Gongronella butleri IFO 8080 at pH 5.5 to 6.5 and 24 to 27 deg. C for 4 days and followed by culturing in a batch bioreactor at pH 5.5 to 6.5, 27 deg. C for 2 days with agitation of 600 to 650 rpm; filtering and washing with ethanol the cultured fungal mycelia, and freeze-drying the fungal mycelia; pulverizing the dried fungal mycelia and extracting them with 1N-NaOH at 121 deg. C for 15 minutes; filtering and washing with ethanol the alkali insoluble material and freeze-drying it; pulverizing the dried alkali insoluble materials(AIM) and treating it with acid; centrifuging the acid treated AIM and filtering the supernatant thereof; adding 20% NaOH into the filtered solution; and centrifuging, neutralizing and freeze-drying the precipitate to obtain fungal chitosan(FCS).
    • 目的:提供从真菌生产壳聚糖的方法,从而简化了壳聚糖的生产过程,减少了废水和废物的数量。 构成:从真菌制备壳聚糖的方法包括以下步骤:在pH5.5至6.5和24至27度下培养耻垢菌IFO 5301或Gongronella butleri IFO 8080。 培养4天,然后在分批生物反应器中在pH 5.5至6.5,27度下培养。 在600至650rpm的搅拌下搅拌2天; 用乙醇过滤和洗涤培养的真菌菌丝体,并冷冻干燥真菌菌丝体; 粉碎干燥的真菌菌丝体,并在121℃用1N-NaOH萃取。 C 15分钟; 用乙醇过滤和洗涤碱不溶物并冷冻干燥; 粉碎干燥的碱不溶物(AIM)并用酸处理; 离心酸处理的AIM并过滤其上清液; 向过滤的溶液中加入20%NaOH; 离心,中和冷冻干燥沉淀,得到真菌壳聚糖(FCS)。
    • 3. 发明公开
    • 균류로부터 생산된 키토산을 이용한 늦죽순 병조림의 백탁방지방법
    • 从真菌生产壳聚糖的方法及使用枸杞子防止BAMBOO-SHOOT瓶的润湿的方法
    • KR1020030097166A
    • 2003-12-31
    • KR1020020034352
    • 2002-06-19
    • 강수태박광식공청식경상대학교산학협력단
    • 오광수허종화
    • C12P19/26
    • PURPOSE: A method for producing chitosan from Fungus and a method for preventing turbidness of a bamboo-shoot bottle using the produced chitosan are provided, thereby decreasing the amount of acid and alkali used to decrease the amount of the wastewater and wastes, and mass-producing the chitosan. CONSTITUTION: A method for producing chitosan from Fungus comprises the steps of: culturing Absidia coerulea IFO 5301 or Gongronella butleri IFO 8080 in a medium containing 2.0% of carbon source, 1.0% of organic nitrogen source, 0.5% of inorganic nitrogen source, 0.1% of phosphate source, 0.1% of potassium source, 0.1% of sodium chloride, and 0.01 to 0.05% of metal salt at pH 5.5 to 6.5 and 24 to 30 deg. C for 6 day; and culturing the medium in a fed-batch bioreactor at pH 5.5 to 6.5, 27 deg. C and 600 to 800 rpm for 36 to 48 hours; and isolating and separating chitosan from the cultured Absidia coerulea IFO 5301 or Gongronella butleri IFO 8080. A method for preventing turbidness of a bamboo-shoot bottle comprises adding 10 to 40 mg of the chitosan produced from fungus into a bottle containing boiled bamboo-shoot.
    • 目的:提供从真菌生产壳聚糖的方法和使用生产的壳聚糖防止竹笋瓶浑浊的方法,从而减少用于减少废水和废物量的酸和碱的量, 生产壳聚糖。 构成:从真菌生产壳聚糖的方法包括以下步骤:在含有2.0%碳源,1.0%有机氮源,0.5%无机氮源,0.1%碳酸钾的培养基中培养茜草IFO 5301或Gongronella butleri IFO8080, 磷酸盐源,0.1%钾源,0.1%氯化钠和0.01至0.05%的金属盐,pH 5.5至6.5和24至30度。 C 6天; 并在补料分批生物反应器中在pH 5.5至6.5,27℃培养培养基。 C和600至800rpm,36至48小时; 从培养的茜草IFO 5301或Gongronella butleri IFO8080中分离和分离壳聚糖。防止竹笋浑浊的方法包括将10至40mg由真菌产生的壳聚糖加入到含有笋竹的瓶中。
    • 6. 发明公开
    • 갈변화 방지 및 풍미가 개선된 우렁쉥이 젓갈의 제조방법
    • 降低低密度白蛋白的抑制方法和油脂增强方法
    • KR1020080010532A
    • 2008-01-31
    • KR1020060070609
    • 2006-07-27
    • 경상대학교산학협력단김영아강수태
    • 오광수김영아강수태강정구강진영노태현남기호
    • A23L1/325
    • A23L17/20A23L17/75
    • A preparation method of salted sea squirt is provided to enhance the quality of salted sea squirt by soaking sea squirt in a NaHSO3 solution. A preparation method of salted sea squirt comprises the steps of: (i) shredding sea squirt into 5mm size and soaking it in a mixed solution of 10%(w/w) of salt and 1%(w/w) of sodium erythorbic acid for 20min; (ii) dehydrating the soaked sea squirt and soaking it in a 0.1% NaHSO3 solution for 1min; and (iii) dehydrating the soaked sea squirt, adding 20%(w/w) of a mixture of salted and fermented anchovy sauce and rice gruel, 0.25%(w/w) of lactic acid, 2%(w/w) of ethanol and 6%(w/w) of sorbitol to the dehydrated sea squirt, and then aging the mixture at 5°C for 15day.
    • 提供盐水喷雾剂的制备方法,通过在NaHSO 3溶液中浸泡海绵来提高盐水喷雾的质量。 盐水喷雾剂的制备方法包括以下步骤:(i)将海喷粉碎至5mm尺寸,并将其浸入10%(w / w)盐和1%(w / w)的异抗坏血酸的混合溶液中 20分钟 (ii)使浸泡的海水喷雾器脱水并将其浸入0.1%NaHSO 3溶液中1分钟; 和(iii)将浸泡的海水喷雾器脱水,加入20%(w / w)的咸和发酵的鲥鱼酱和米粉,0.25%(w / w)的乳酸,2%(w / w) 乙醇和6%(w / w)的山梨醇加入到脱水的海水中,然后在5℃下将混合物老化15天。
    • 7. 发明授权
    • 전복 통조림의 가공방법
    • 一种处理ABALONE CAN的方法
    • KR100859848B1
    • 2008-09-23
    • KR1020070060866
    • 2007-06-21
    • 경상대학교산학협력단공청식이성만
    • 김정균오광수공청식이성만엄광식최동진
    • A23B4/00A23B4/02A23B4/023A23B4/005
    • Y02A40/946A23B4/023A23B4/0056
    • A method of preparing canned abalone by addition of bamboo salt and salt during the manufacture is provided to remove a fishy smell without separate seasoning and to give pharmacological efficacy of bamboo salt. Non-skinned or skinned abalone with a pH of 6.20 to 6.75 and the volatile basic nitrogen content in the range 5 to 8mg/100g is used. The non-skinned abalone is washed, heated with steam at 100deg.C for 15 to 25min. The skinned abalone is washed and heated with steam or water at 100deg.C for 15 to 25min. The steamed abalone is cooled to less than 10deg.C, washed with meat broth, dehydrated and dried. In the drying process, the abalone is salted with 6% table salt for 12 to 24hr, rubbed with a brush, washed with meat broth, dehydrated, boiled with hot water at 95deg.C for 1 to 2hr, dried in the shade for 1hr and re-dried at 70deg.C. The drying and re-drying process are repeated for 2 to 3 days. The processed abalone is finally dried for 5 days to a moisture content of 30 to 40%, put into a can, degassed at 95 to 100deg.C for 20 to 30min, poured with 1.0 to 1.5% by weight of table salt and 0.5 to 1.0% by weight of bamboo salt, sealed at 20 to 30cm/Hg, sterilized at 113 to 121deg.C for 40 to 80min and cooled at 38deg.C for 20 to 30min and at room temperature for 12hr.
    • 提供制造期间加入竹盐和盐的罐装鲍鱼的方法,以在没有单独调味的情况下除去鱼腥味,并提供竹盐的药理功效。 使用pH值为6.20〜6.75的非皮肤或皮肤鲍鱼,挥发性碱性氮含量在5〜8mg / 100g的范围内。 将无皮鲍鱼洗涤,100℃蒸汽加热15〜25分钟。 将皮肤鲍鱼用蒸汽或水在100℃下洗涤15至25分钟。 蒸鲍鱼冷却至10℃以下,用肉汤洗净,脱水干燥。 在干燥过程中,鲍鱼用6%盐水浸盐12〜24小时,用刷子擦拭,用肉汤洗涤,脱水,用95℃的热水煮沸1〜2小时,在阴凉处干燥1小时 并在70℃重新干燥。 干燥和再干燥过程重复2至3天。 将加工的鲍鱼最后干燥5天,水份含量为30〜40%,放入罐中,在95〜100℃下脱气20〜30分钟,倒入1.0〜1.5重量%的餐盐,0.5〜 竹盐1.0重量%,密封于20〜30cm / Hg,113〜121℃灭菌40〜80分钟,在38℃下冷却20〜30分钟,室温12小时。
    • 8. 发明公开
    • 풍미가 개선된 진주조개육 엑기스를 이용한 천연조미료의제조방법
    • 制备包含梨形肌肉提取物的自然风味的增强方法
    • KR1020080069463A
    • 2008-07-28
    • KR1020070007221
    • 2007-01-23
    • 경상대학교산학협력단강수태이성만지민 쑤
    • 오광수김정균강수태강정구이성만지민쑤
    • A23L1/221A23L1/33A23L1/231
    • A23L27/10A23L27/21A23L27/88A23V2002/00A23V2250/2042
    • A method of manufacturing natural seasonings is provided to prepare natural seasonings with characteristic flavor and taste and functionality of pearl oyster muscle and utilizes pearl oyster muscle which is hitherto been discarded. Natural seasonings are prepared by the steps of: mixing pearl oyster muscle extract(Brix 20deg.), 0.4M glucose, 0.4M glycine and 0.4M cystine in a weight ratio of 4:2:1:1 and reacting at 120 to 150deg.C for 140 to 150min while maintaining a pH of 6.9 to 7.6; and additionally adding oyster concentrates thereto. The pearl oyster muscle extract is obtained by the steps of: mixing pearl oyster muscle with 2 to 3 times the weight of water; blanching at 90 to 100deg.C for 3 to 10min; adding Alcalase thereto and heating at 50 to 60deg.C for 3 to 5hr while stirring; heating at 90 to 100deg.C for 3 to 10min to deactivate the Alcalase; adding exopeptidase and hydrolyzing at 40 to 50deg.C for 3 to 5hr while stirring; heating at 90 to 100deg.C for 3 to 10min to deactivate the exopeptidase; and separating the residue, centrifuging and concentrating.
    • 提供天然调味料的制备方法,以制备具有珍珠牡蛎肌的特征风味和味道及功能的天然调味品,并利用迄今为止被丢弃的珍珠牡蛎肌。 天然调味料的制备步骤如下:将珍珠牡蛎肌提取物(Brix 20g),0.4M葡萄糖,0.4M甘氨酸和0.4M胱氨酸以4:2:1:1的重量比混合并在120至150℃下反应。 C保持140至150分钟,同时保持6.9至7.6的pH; 并另外添加牡蛎浓缩物。 珍珠牡蛎肌提取物通过以下步骤获得:将珍珠牡蛎肌与水重量的2至3倍混合; 漂洗90〜100℃3〜10min; 向其中加入Alcalase并在搅拌下在50至60℃加热3至5小时; 在90〜100℃下加热3〜10分钟使Alcalase失活; 加入外肽酶,并在搅拌下于40〜50℃水解3〜5小时; 在90〜100℃加热3〜10分钟,使外肽酶失活; 并分离残留物,离心和浓缩。