会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 11. 发明公开
    • 카피수 보존 RNA 분석 방법
    • RNA复制数量保存RNA分析方法
    • KR1020160138168A
    • 2016-12-02
    • KR1020167029321
    • 2015-03-20
    • 렉소겐 게엠베하
    • 몰,파멜라
    • C12Q1/68
    • C12Q1/6806C12N15/1096C12Q1/6851C12Q1/6853C12Q1/686C12Q2521/101C12Q2521/107C12Q2521/327C12Q2525/155C12Q2525/173C12Q2525/179C40B20/04C12Q2537/16
    • 본발명은주형 RNA를수득한후, 주형 RNA의미리선택된 3' 말단핵산영역에제1 올리고뉴클레오티드프라이머를어닐링시키는단계, 주형에특이적인방식으로제1 올리고뉴클레오티드프라이머를신장시켜제1 신장된가닥을수득하는단계, RNA 주형을제거하는단계, 하나이상의추가의올리고뉴클레오티드프라이머를제1 신장된가닥에어닐링시키는단계, 제1 신장된가닥에어닐링된폴리뉴클레오티드의가닥치환없이, 또는치환된가닥을파괴하는폴리머라제를사용하여주형에특이적인방식으로하나이상의추가의올리고뉴클레오티드프라이머를신장시켜추가의신장생성물을생성하는단계, 제1 올리고뉴클레오티드프라이머에상보적인, 신장된핵산부분을포함하는상기추가의신장생성물의신장생성물을단리시키고/거나, 증폭시키는단계를포함하는, 주형 RNA 분자의증폭된핵산부분을생성하는방법; 뿐만아니라, 상기방법을수행하기위한키트를제공한다.
    • 本发明提供了生成模板RNA分子的扩增核酸部分的方法,其包括在获得模板RNA之后,在模板RNA的预选的3'末端核酸区退火第一寡核苷酸引物,延伸第一寡核苷酸 引物,从而获得第一细长链,去除RNA模板,将一个或多个另外的寡核苷酸引物退火至第一细长链,以模板特异性方式延伸一个或多个其它寡核苷酸引物而不退出多核苷酸的链置换 或使用破坏位移链的聚合酶,由此产生进一步的延伸产物,分离和/或扩增所述另外的延伸产物的延伸产物,其包含与第一寡核苷酸引物互补的核酸部分; 以及用于执行该方法的套件。
    • 18. 发明公开
    • 기지의 서열에 인접한 미지의 DNA 서열을 증폭하는 방법
    • 用于放大不知名的DNA序列的方法来了解知识序列
    • KR1020090005183A
    • 2009-01-12
    • KR1020087027939
    • 2006-05-04
    • 주식회사 씨젠
    • 천종윤
    • C12Q1/68C12N15/09
    • C12Q1/6844C12Q1/6853C12N15/09C12Q2527/107C12Q2525/179C12Q2525/101C12Q2525/185C12Q2525/161
    • A method for amplifying the unknown nucleotide sequence adjacent to the known nucleotide sequence, and a kit used in the method are provided to remove the high background problem completely and to improve confidence. A method for amplifying the unknown nucleotide sequence adjacent to the known nucleotide sequence comprises the steps of first-stage amplifying the unknown nucleotide sequence with the first degenerate DNA working annealing control primer (DW-ACP) at a first annealing temperature where the first degenerate DW-ACP acts as a primer by carrying out at least one cycle comprising primer annealing, primer extension and denaturing processes; and second-stage amplifying the amplified product at a second annealing temperature higher than the first annealing temperature by carrying out at least one cycle comprising primer annealing using the DW-ACP and the first target-specific primer (TSP), primer extension and denaturing processes, wherein the first degenerate DW-ACP comprises the degenerate random nucleotide hybrided with the known nucleotide sequence and the hybrid nucleotide sequence complementary to the one position of the known nucleotide sequence.
    • 提供了用于扩增与已知核苷酸序列相邻的未知核苷酸序列的方法,以及用于该方法的试剂盒,以完全去除高背景问题并提高置信度。 用于扩增与已知核苷酸序列相邻的未知核苷酸序列的方法包括以第一退火温度在第一退火温度下用第一退化DNA工作退火控制引物(DW-ACP)首先扩增未知核苷酸序列的步骤,其中第一退化DW -ACP通过进行包括引物退火,引物延伸和变性过程的至少一个循环作为引物; 通过使用DW-ACP和第一靶特异性引物(TSP),引物延伸和变性过程进行至少一个包括引物退火的循环,在高于第一退火温度的第二退火温度下对扩增产物进行第二阶段扩增 其中所述第一简并DW-ACP包含与已知核苷酸序列杂交的简并随机核苷酸和与所述已知核苷酸序列的一个位置互补的杂交核苷酸序列。
    • 20. 发明公开
    • 스캐닝 프로브 현미경 및 나노코드를 이용하여 핵산을검출하는 방법 및 조성물
    • 使用扫描探针显微镜和纳米级检测核酸的方法和组合物
    • KR1020060135719A
    • 2006-12-29
    • KR1020067014916
    • 2004-12-28
    • 인텔 코포레이션
    • 버린앤드류야마카와미네오
    • C12Q1/68B82Y5/00
    • C12Q1/6874B82Y5/00B82Y10/00C12Q1/6876C12Q2525/179C12Q2563/155C12Q2565/601
    • A method for determining a nucleotide sequence of a nucleic acid is provided that includes contacting the nucleic acid with a series of labeled oligonucleotides for binding to the nucleic acid, wherein each labeled oligonucleotide includes a known nucleotide sequence and a molecular nanocode. The nanocode of an isolated labeled oligonucleotides that binds to the nucleic acid is then detected using SPM. Nanocodes of the present invention in certain aspects include detectable features beyond the arrangement of tags that encode information about the barcoded object, which assist in detecting the tags that encode information about the barcoded object. The detectable features include structures of a nanocode or associated with a nanocode, referred to herein as detectable feature tags, for error checking/error-correction, encryption, and data reduction/compression.
    • 提供了用于确定核酸的核苷酸序列的方法,其包括使核酸与一系列用于结合核酸的标记的寡核苷酸接触,其中每个标记的寡核苷酸包括已知的核苷酸序列和分子纳代码。 然后使用SPM检测与核酸结合的分离的标记寡核苷酸的纳代码。 在某些方面,本发明的纳代码包括超过编码关于条形码对象的信息的标签布置的可检测特征,其有助于检测编码关于条形码对象的信息的标签。 可检测特征包括用于错误校验/纠错,加密和数据缩减/压缩的纳代码或与纳代码相关联的结构,这里称为可检测特征标签。