会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 9. 发明公开
    • GENERATION OF INDUCED PLURIPOTENT STEM (IPS) CELLS
    • 埃策ON ON ON TEN EN EN EN EN)))))))
    • EP2297300A1
    • 2011-03-23
    • EP09753663.5
    • 2009-05-26
    • Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.
    • KIM, Jeong, BeomZAEHRES, HolmSCHÖLER, Hans, Robert
    • C12N5/06
    • C12N5/0696A61D19/04C12N15/85C12N15/8509C12N15/873C12N2501/602C12N2501/603C12N2501/604C12N2501/606C12N2502/13C12N2506/08C12N2506/13C12N2510/00C12N2799/027G01N33/5073
    • The present invention relates to a method of generating an induced pluripotent stem (iPS) cell comprising the step of introducing into a target cell one or two coding sequences each giving rise upon transcription to a factor that contributes to the reprogramming of said target cell into an induced pluripotent stem cell and selected from Oct3/4 or a factor belonging to the Myc, Klf and Sox families of factors, wherein the target cell endogenously expresses at least the factors that are not encoded by the coding sequences to be introduced and selected from Oct3/4 or factors belonging to the Myc, Klf and Sox families of factors, and wherein the cell resulting from the introduction of the one or two coding sequences expresses the combination of factor Oct3/4 and at least one factor of each family of factors selected from the group of Myc, Klf and Sox. Furthermore, the present invention relates to an induced pluripotent stem cell generated by the method of the invention and a method of identifying a compound that contributes to the reprogramming of a target cell into an induced pluripotent stem cell. Also, a method of generating a transgenic non-human animal and a composition comprising an iPS cell generated by the method of the present invention for gene therapy, regenerative medicine, cell therapy or drug screening are envisaged.
    • 本发明涉及产生诱导多能干细胞(iPS)细胞的方法,该方法包括以下步骤:向靶细胞中引入一个或两个编码序列,每个编码序列在转录时引起有助于将所述靶细胞重新编程成为 诱导多能干细胞,选自Oct3 / 4或属于Myc,Klf和Sox家族因子的因子,其中目标细胞至少内源性表达至少不被引入并选自Oct3的编码序列编码的因子 / 4或属于Myc,Klf和Sox家族因子的因子,并且其中由引入一个或两个编码序列产生的细胞表达因子Oct3 / 4与所选因子的至少一个因子的组合 从Myc,Klf和Sox组。 此外,本发明涉及通过本发明的方法产生的诱导多能干细胞和鉴定有助于将靶细胞重编程为诱导多能干细胞的化合物的方法。 此外,设想了产生转基因非人动物的方法和包含通过本发明的方法产生的用于基因治疗,再生医学,细胞治疗或药物筛选产生的iPS细胞的组合物。