专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP1691196A1 MICROWELL ARRAY CHIP AND ITS MANUFACTURING METHOD 有权转让
标题翻译： MICROWELL-ARRAY-CHIP UND VERFAHREN ZU DESSEN HERSTELLUNG
公开(公告)号：EP1691196A1
公开(公告)日：2006-08-16
申请号：EP04773462.9
申请日：2004-09-22
申请人： Toyama Prefecture , Muraguchi, Atsushi , Kishi, Hiroyuki , Tokimitsu, Yoshiharu , Kondo, Sachiko
发明人： MURAGUCHI, Atsushi , KISHI, Hiroyuki , Tokimitsu, Yoshiharu , KONDO, Sachiko , Obata, Tsutomu , Fujiki, Satoshi , Yokoyama, Yoshiyuki , Nabesawa, Hirofumi , Takabayashi, Sotohiro , Tanino, Katsumi
IPC分类号： G01N33/53 , G01N37/00
CPC分类号： G01N33/54366 , A61K35/12 , A61K48/00 , B01J19/0046 , B01J2219/00659 , C12M23/12 , G06F19/00
摘要： A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein microwell markers are present on the same substrate surface as the openings of the microwells. A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein protrusions are present in the openings of said microwells so as to narrow said openings. A method for manufacturing the microwell array chip. The method comprises the steps of: forming a film on at least one principal surface of a substrate; applying a resist coating on the film that has been formed; exposing the resist surface through a mask having a microwell pattern and removing uncured portions of resist; etching the exposed portions of said film and substrate to fabricate wells in the form of a microwell array; and removing the resist. A microwell array chip made of silicon and having multiple microwells, each microwell being used to store a single specimen organic cell, wherein each microwell is of a size and shape holding just one organic cell.
摘要翻译：一种微孔阵列芯片，其在基底的主表面上具有多个微孔，所述微孔具有允许在每个微孔中仅存储单个有机细胞的形状和尺寸，其中微孔标记物存在于相同的基底表面上，微孔。一种微孔阵列芯片，其在基底的主表面上具有多个微孔，所述微孔具有允许在每个微孔中仅存储单个有机细胞的形状和尺寸，其中突起存在于所述微孔的开口中以便缩窄说开口。一种微孔阵列芯片的制造方法。该方法包括以下步骤：在衬底的至少一个主表面上形成膜; 在已经形成的膜上涂覆抗蚀涂层; 通过具有微孔图案的掩模曝光抗蚀剂表面并除去未固化的抗蚀剂部分; 蚀刻所述膜和衬底的暴露部分以制造微孔阵列形式的阱; 并除去抗蚀剂。一种由硅制成并具有多个微孔的微孔阵列芯片，每个微孔用于存储单个样品有机电池，其中每个微孔具有仅具有一个有机电池的尺寸和形状。

2. 发明公开

EP1614749A1 METHOD OF CONSTRUCTING HYBRIDOMA PRODUCING ANTIGEN-SPECIFIC ANTIBODY USING SINGLE ANTIGEN-SPECIFIC B LYMPHOCYTE AND METHOD OF PRODUCING MONOCLONAL ANTIBODY 审中-公开
标题翻译： PROCESS FOR抗原特异性抗体的杂交瘤制造的构建使用用于生产单克隆抗体的一个抗原特异性B淋巴细胞和方法
公开(公告)号：EP1614749A1
公开(公告)日：2006-01-11
申请号：EP04723704.5
申请日：2004-03-26
申请人： Toyama New Industry Organizaion , Muraguchi, Atsushi , Kishi, Hiroyuki , Tamiya, Eiichi , Suzuki, Masayasu
发明人： MURAGUCHI, Atsushi , KISHI, Hiroyuki , TAMIYA, Eiichi , SUZUKI, Masayasu , TOHBO, Kihachiro , UENO, Minoru , NAKAZATO, Hiroyoshi
IPC分类号： C12N15/08 , C12P21/08
CPC分类号： C07K16/00 , C12N5/163
摘要： A method for producing a monoclonal antibody and antigen-specific antibody-producing hybridomas using a single antigen-specific B lymphocyte is provided. The method for producing antigen-specific antibody-producing hybridomas comprises selecting a single B lymphocyte reacting specifically with a certain antigen, an "antigen-specific B lymphocyte", culturing the antigen-specific B lymphocyte selected, and fusing the cultured antigen-specific B lymphocyte with myeloma cells to obtain the hybridomas. The monoclonal antibody is produced with thus obtained hybridomas.
摘要翻译：提供了一种用于使用单个抗原特异性B淋巴细胞生产的单克隆抗体和抗原特异性抗体的杂交瘤的方法。用于生产抗原特异性抗体的杂交瘤的方法，包括选择一个单一的B淋巴细胞与某种抗原的，“抗原特异性B淋巴细胞”特异性起反应，培养所述抗原特异性B淋巴细胞选择，并且融合培养的抗原特异性B 淋巴细胞与骨髓瘤细胞，以获得杂交瘤。的单克隆抗体产生与这样获得的杂交瘤。

3. 发明授权

EP2184345B1 CELL SCREENING METHOD 有权转让
标题翻译： CELL筛选过程
公开(公告)号：EP2184345B1
公开(公告)日：2016-03-23
申请号：EP08792087.2
申请日：2008-08-01
申请人： Toyama Prefecture , Valneva
发明人： JIN, Aishun , KISHI, Hiroyuki , MURAGUCHI, Atsushi , OBATA, Tsutomu
IPC分类号： G01N33/50 , G01N33/543 , G01N33/558 , G01N33/53 , G01N33/15 , C12M1/34 , C12M1/18 , C12Q1/02
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.

4. 发明公开

EP1942179A1 CHIP PROVIDED WITH FILM HAVING HOLE PATTERN WITH THE USE OF THERMORESPONSIVE POLYMER AND METHOD OF PRODUCING THE SAME 有权
标题翻译：与孔图形芯片提供，使用其制造的温度下反应性聚合物和方法FILM
公开(公告)号：EP1942179A1
公开(公告)日：2008-07-09
申请号：EP06798370.0
申请日：2006-09-27
申请人： Toyama Prefecture , Nissan Chemical Industries, Ltd.
发明人： TAMIYA, Eiichi , YOKOYAMA, Yoshiyuki , FUJIKI, Satoshi , TANINO, Katsumi , MURAGUCHI, Atsushi , KISHI, Hiroyuki , TOKIMITSU, Yoshiharu , YAMAMURA, Shohei
IPC分类号： C12M3/00 , C12N5/06
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2535/10 , C12N2537/10 , C12N2539/10 , Y10T428/24273 , Y10T436/25 , Y10T436/25375
摘要： [PROBLEMS] To provide a novel chip useful for tretaing cells and the like which has a mechanism and a structure wherein the size of a hole pattern is arbitrarily changed so that cells can easily move in and get out from the hole in scattering or collecting cells but can hardly get out from the hole during washing or antigen-stimulation. [MEANS FOR SOLVING PROBLEMS A chip comprising a crosslinked product of a temperature-responsive polymer as a constituting member and being provided with a film having a hole pattern on the surface of a baseboard. A method of producing a chip which comprises a crosslinked product of a temperature-responsive polymer as a constituting member and is provided with a film having a hole pattern on the surface of the baseboard. This method comprises applying a composition containing a crosslinkable temperature-responsive polymer, a composition containing a crosslinkable temperature-responsive polymer and a crosslinking agent or a composition containing a temperature-responsive polymer and a crosslinking agent on the surface of a baseboard to thereby form a coating film, crosslinking the coating film to thereby form the crosslinked product as described above and then forming a hole pattern on the coating film of the crosslinked product.
摘要翻译： [问题]提供一种具有一个机构的新型芯片等，用于tretaing细胞是有用的，并和结构worin孔图案的尺寸被任意地改变，以便做细胞可以很容易地移进和散射或收集细胞从孔走出但几乎不能从孔洗涤或抗原刺激过程中全身而退。用于解决技术问题的芯片，包括一个温度响应性聚合物作为构成部件的交联产物和设置有具有基板的表面上的孔图案的膜。制造芯片，它包括一个温度响应性聚合物的交联产物，其为构成构件和设置有具有该基板的表面上的孔图案的膜的方法。该方法包括将含有可交联的温度响应性聚合物的组合物，含有交联性温度响应性聚合物和交联剂或含有温度响应性聚合物的组合物和基板的表面上的交联剂的组合物，从而形成涂层电影，交联的涂膜，从而形成如上述，然后描述在所述膜交联产品的涂层的孔图案的交联产物。

5. 发明授权

EP1942179B1 CHIP PROVIDED WITH FILM HAVING HOLE PATTERN WITH THE USE OF THERMORESPONSIVE POLYMER AND METHOD OF PRODUCING THE SAME 有权
标题翻译：提供具有孔图案的膜以及使用热敏聚合物的芯片及其制造方法
公开(公告)号：EP1942179B1
公开(公告)日：2017-05-10
申请号：EP06798370.0
申请日：2006-09-27
申请人： Toyama Prefecture , Nissan Chemical Industries, Ltd.
发明人： TAMIYA, Eiichi , YOKOYAMA, Yoshiyuki , FUJIKI, Satoshi , TANINO, Katsumi , MURAGUCHI, Atsushi , KISHI, Hiroyuki , TOKIMITSU, Yoshiharu , YAMAMURA, Shohei
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N2533/12 , C12N2535/10 , C12N2537/10 , C12N2539/10 , Y10T428/24273 , Y10T436/25 , Y10T436/25375

6. 发明授权

EP1691196B1 MICROWELL ARRAY CHIP AND ITS MANUFACTURING METHOD 有权转让
标题翻译： MICROWELL-阵列芯片及其制造方法
公开(公告)号：EP1691196B1
公开(公告)日：2012-12-26
申请号：EP04773462.9
申请日：2004-09-22
申请人： Toyama Prefecture , Vivalis
发明人： MURAGUCHI, Atsushi , KISHI, Hiroyuki , Tokimitsu, Yoshiharu , KONDO, Sachiko , Obata, Tsutomu , Fujiki, Satoshi , Yokoyama, Yoshiyuki , Nabesawa, Hirofumi , Takabayashi, Sotohiro , Tanino, Katsumi
IPC分类号： G01N33/53 , G01N37/00
CPC分类号： G01N33/54366 , A61K35/12 , A61K48/00 , B01J19/0046 , B01J2219/00659 , C12M23/12 , G06F19/00
摘要： A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein microwell markers are present on the same substrate surface as the openings of the microwells. A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein protrusions are present in the openings of said microwells so as to narrow said openings. A method for manufacturing the microwell array chip. The method comprises the steps of: forming a film on at least one principal surface of a substrate; applying a resist coating on the film that has been formed; exposing the resist surface through a mask having a microwell pattern and removing uncured portions of resist; etching the exposed portions of said film and substrate to fabricate wells in the form of a microwell array; and removing the resist. A microwell array chip made of silicon and having multiple microwells, each microwell being used to store a single specimen organic cell, wherein each microwell is of a size and shape holding just one organic cell.

7. 发明公开

EP2184345A1 CELL SCREENING METHOD 有权转让
标题翻译： ZELLEN筛选，VERFAHREN
公开(公告)号：EP2184345A1
公开(公告)日：2010-05-12
申请号：EP08792087.2
申请日：2008-08-01
申请人： Toyama Prefecture , SC World Inc.
发明人： JIN, Aishun , KISHI, Hiroyuki , MURAGUCHI, Atsushi , OBATA, Tsutomu
IPC分类号： C12M1/34 , C12M1/18 , C12Q1/02 , G01N33/15 , G01N33/50 , G01N33/53
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.
摘要翻译：提供了一种允许同时测量保持在芯片上的10,000多个抗原刺激的淋巴细胞的反应性质和单个细胞状态的单独测定的方法和手段。微孔阵列包括多个孔和在基底构件的一个主表面上的涂层，所述孔具有允许仅将单个细胞进入每个孔的尺寸。能够与孔中包含的细胞产生的物质结合的物质的涂层存在于孔周围的主表面上。筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在上述微孔阵列的孔中; 将涂层和孔浸入培养液中，并使培养液中的物质从孔扩散到涂层中的状态下培养细胞; 将标本物质特异性地与存在于样品池中的靶细胞产生的物质结合到涂层上; 并且通过所述标记物质检测由所述涂层中的物质结合的靶细胞产生的物质以指定所述靶细胞。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式