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1. 发明公开

EP1566635A4 MICROWELL ARRAY CHIP FOR DETECTING ANTIGEN-SPECIFIC LYMPHOCYTE, METHOD OF DETECTING ANTIGEN-SPECIFIC LYMPHOCYTE AND METHOD OF CLONING ANTIGEN-SPECIFIC LYMPHOCYTE ANTIGEN RECEPTOR GENE 无效
标题翻译： MICRO WELL-ARRAY芯片抗原检测特异性淋巴细胞的，方法抗原特异性淋巴细胞中克隆抗原受体基因抗原特异性淋巴细胞的检测和方法
公开(公告)号：EP1566635A4
公开(公告)日：2006-12-27
申请号：EP03812277
申请日：2003-09-30
申请人： MURAGUCHI ATSUSHI , KISHI HIROYUKI , TAMIYA EIICHI , SUZUKI MASAYASU
发明人： MURAGUCHI ATSUSHI , KISHI HIROYUKI , TAMIYA EIICHI , SUZUKI MASAYASU
IPC分类号： C07K16/08 , C40B60/14 , G01N33/53 , G01N33/543 , G01N33/569 , G01N33/50 , C12M1/34 , C12Q1/02 , G01N33/15 , G01N37/00
CPC分类号： C07K16/082 , B01J2219/00317 , B01J2219/00743 , C07K2317/21 , C40B60/14 , G01N33/5302 , G01N33/54366 , G01N33/56972
摘要： A microwell array chip having plural microwells, containing a test lymphocyte in each microwell and being used for detecting a single antigen-specific lymphocyte, wherein the microwells have such a shape and size that only one lymphocyte is contained in each microwell. A method of detecting an antigen-specific lymphocyte which comprises adding an antigen to each of the microwells of this microwell array chip, thus stimulating the cells and detecting a cell reacting with the antigen.

2. 发明公开

EP2365331A3 Method to select a single antigen-specfic lymphocyte. 有权转让
标题翻译：选择单一抗原特异性淋巴细胞的方法。
公开(公告)号：EP2365331A3
公开(公告)日：2011-11-23
申请号：EP10184204.5
申请日：2003-09-30
申请人： Muraguchi, Atsushi , Kishi, Hiroyuki , Suzuki, Masayasu , Tamiya, Eiichi
发明人： Muraguchi, Atsushi , Kishi, Hiroyuki , Suzuki, Masayasu , Tamiya, Eiichi
IPC分类号： G01N33/50 , G01N33/15 , G01N37/00 , C12M1/34 , C12Q1/02 , C07K16/08 , G01N33/53 , G01N33/543 , G01N33/569
CPC分类号： C07K16/082 , B01J2219/00317 , B01J2219/00743 , C07K2317/21 , C40B60/14 , G01N33/5302 , G01N33/54366 , G01N33/56972
摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.
摘要翻译：微孔阵列芯片具有多个微孔并且用于在每个微孔中包含单个淋巴细胞样本并且以单个单位检测抗原特异性淋巴细胞; 其中所述微孔阵列芯片具有其中每个微孔中仅包含一个淋巴细胞的形状和尺寸。一种检测抗原特异性淋巴细胞的方法，包括在上述微孔阵列芯片中的每个微孔中加入抗原，刺激淋巴细胞样本，并检测淋巴细胞样本与抗原反应的步骤。

3. 发明公开

EP1566635A1 MICROWELL ARRAY CHIP FOR DETECTING ANTIGEN-SPECIFIC LYMPHOCYTE, METHOD OF DETECTING ANTIGEN-SPECIFIC LYMPHOCYTE AND METHOD OF CLONING ANTIGEN-SPECIFIC LYMPHOCYTE ANTIGEN RECEPTOR GENE 无效
标题翻译： MICRO WELL-ARRAY芯片抗原检测特异性淋巴细胞的，方法抗原特异性淋巴细胞中克隆抗原受体基因抗原特异性淋巴细胞的检测和方法
公开(公告)号：EP1566635A1
公开(公告)日：2005-08-24
申请号：EP03812277.6
申请日：2003-09-30
申请人： Muraguchi, Atsushi , Kishi, Hiroyuki , Tamiya, Eiichi , Suzuki, Masayasu
发明人： Muraguchi, Atsushi , Kishi, Hiroyuki , Tamiya, Eiichi , Suzuki, Masayasu
IPC分类号： G01N33/50 , G01N33/15 , G01N37/00 , C12M1/34 , C12Q1/02
CPC分类号： C07K16/082 , B01J2219/00317 , B01J2219/00743 , C07K2317/21 , C40B60/14 , G01N33/5302 , G01N33/54366 , G01N33/56972
摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.
摘要翻译：微孔阵列芯片做具有多个微孔中，并且被用于在包含每个微孔的单个淋巴细胞标本并检测在单个单位抗原特异性淋巴细胞; worin微孔阵列芯片是一个形状，并且其中仅一个淋巴细胞包含在每个微孔的尺寸。检测抗原特异性淋巴细胞，其包括在上述微孔阵列芯片添加抗原每个微孔，刺激淋巴细胞样品，并检测样品的淋巴细胞与该抗原反应的步骤的方法。

4. 发明公开

EP2365331A2 Method to select a single antigen-specfic lymphocyte. 有权转让
标题翻译： Vefahren zur Klonierung eines Antigenspezifischen Lymphozyten zu klonen。
公开(公告)号：EP2365331A2
公开(公告)日：2011-09-14
申请号：EP10184204.5
申请日：2003-09-30
申请人： Muraguchi, Atsushi , Kishi, Hiroyuki , Suzuki, Masayasu , Tamiya, Eiichi
发明人： Muraguchi, Atsushi , Kishi, Hiroyuki , Suzuki, Masayasu , Tamiya, Eiichi
IPC分类号： G01N33/50 , G01N33/15 , G01N37/00 , C12M1/34 , C12Q1/02 , C07K16/08
CPC分类号： C07K16/082 , B01J2219/00317 , B01J2219/00743 , C07K2317/21 , C40B60/14 , G01N33/5302 , G01N33/54366 , G01N33/56972
摘要： A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method of detecting antigen-specific lymphocytes comprising the steps of adding antigen to each microwell in the above microwell array chip, stimulating the lymphocyte specimen, and detecting lymphocyte specimens reacting with the antigen.
摘要翻译：具有多个微孔并用于在每个微孔中含有单个淋巴细胞标本的微孔阵列芯片，并以单个单元检测抗原特异性淋巴细胞; 其中微孔阵列芯片的形状和尺寸在每个微孔中仅包含一个淋巴细胞。一种检测抗原特异性淋巴细胞的方法，包括以下步骤：在上述微孔阵列芯片中的每个微孔中加入抗原，刺激淋巴细胞标本，以及检测与抗原反应的淋巴细胞标本。

5. 发明公开

EP1614749A4 METHOD OF CONSTRUCTING HYBRIDOMA PRODUCING ANTIGEN-SPECIFIC ANTIBODY USING SINGLE ANTIGEN-SPECIFIC B LYMPHOCYTE AND METHOD OF PRODUCING MONOCLONAL ANTIBODY 审中-公开
标题翻译： PROCESS FOR抗原特异性抗体的杂交瘤制造的构建使用用于生产单克隆抗体的一个抗原特异性B淋巴细胞和方法
公开(公告)号：EP1614749A4
公开(公告)日：2006-10-25
申请号：EP04723704
申请日：2004-03-26
申请人： TOYAMA NEW INDUSTRY ORGANIZAIO , MURAGUCHI ATSUSHI , KISHI HIROYUKI , TAMIYA EIICHI , SUZUKI MASAYASU
发明人： MURAGUCHI ATSUSHI , KISHI HIROYUKI , TAMIYA EIICHI , SUZUKI MASAYASU , TOHBO KIHACHIRO , UENO MINORU , NAKAZATO HIROYOSHI
IPC分类号： C12N15/08 , C07K16/00 , C12N5/16 , C12P21/08
CPC分类号： C07K16/00 , C12N5/163
摘要： It is intended to provide a method of constructing a hybridoma producing an antigen-specific antibody with the use of an antigen-specific B lymphocyte and a method of producing a monoclonal antibody. The method of constructing a hybridoma producing an antigen-specific antibody comprises selecting a B lymphocyte reacting specifically with a certain antigen (an antigen-specific B lymphocyte), culturing the thus selected antigen-specific B lymphocyte, and fusing the antigen-specific B lymphocytes having proliferated by the culture with myeloma cells to give hybridomas. Using a hybridoma constructed by this method, a monoclonal antibody is produced.

6. 发明公开

EP1614749A1 METHOD OF CONSTRUCTING HYBRIDOMA PRODUCING ANTIGEN-SPECIFIC ANTIBODY USING SINGLE ANTIGEN-SPECIFIC B LYMPHOCYTE AND METHOD OF PRODUCING MONOCLONAL ANTIBODY 审中-公开
标题翻译： PROCESS FOR抗原特异性抗体的杂交瘤制造的构建使用用于生产单克隆抗体的一个抗原特异性B淋巴细胞和方法
公开(公告)号：EP1614749A1
公开(公告)日：2006-01-11
申请号：EP04723704.5
申请日：2004-03-26
申请人： Toyama New Industry Organizaion , Muraguchi, Atsushi , Kishi, Hiroyuki , Tamiya, Eiichi , Suzuki, Masayasu
发明人： MURAGUCHI, Atsushi , KISHI, Hiroyuki , TAMIYA, Eiichi , SUZUKI, Masayasu , TOHBO, Kihachiro , UENO, Minoru , NAKAZATO, Hiroyoshi
IPC分类号： C12N15/08 , C12P21/08
CPC分类号： C07K16/00 , C12N5/163
摘要： A method for producing a monoclonal antibody and antigen-specific antibody-producing hybridomas using a single antigen-specific B lymphocyte is provided. The method for producing antigen-specific antibody-producing hybridomas comprises selecting a single B lymphocyte reacting specifically with a certain antigen, an "antigen-specific B lymphocyte", culturing the antigen-specific B lymphocyte selected, and fusing the cultured antigen-specific B lymphocyte with myeloma cells to obtain the hybridomas. The monoclonal antibody is produced with thus obtained hybridomas.
摘要翻译：提供了一种用于使用单个抗原特异性B淋巴细胞生产的单克隆抗体和抗原特异性抗体的杂交瘤的方法。用于生产抗原特异性抗体的杂交瘤的方法，包括选择一个单一的B淋巴细胞与某种抗原的，“抗原特异性B淋巴细胞”特异性起反应，培养所述抗原特异性B淋巴细胞选择，并且融合培养的抗原特异性B 淋巴细胞与骨髓瘤细胞，以获得杂交瘤。的单克隆抗体产生与这样获得的杂交瘤。

7. 发明公开

EP1500933A1 DEVICE, METHOD, AND KIT FOR GENE DETECTION 审中-公开
标题翻译： VORRICHTUNG，VERFAHREN UND KIT ZUM GENNACHWIS
公开(公告)号：EP1500933A1
公开(公告)日：2005-01-26
申请号：EP03717688.0
申请日：2003-04-22
申请人： Hokuto Scientific Industry, Co., Ltd. , Tamiya, Eiichi
发明人： Tamiya, Eiichi, Tamiya Laboratory , Kobayashi, Masaaki, Tamiya Laboratory , NAGASHIO, Ichiro
IPC分类号： G01N33/53 , C12M1/00
CPC分类号： B01L3/502 , B01L3/50825 , B01L2300/042 , B01L2300/047 , B01L2300/087 , B01L2400/0683
摘要： A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber. The partition member is capable of unsealing; the reaction chamber is comprised of at least two members capable of forming a sealed reaction chamber by mutually fitting an opening means provided therein; and the detection chamber or reaction chamber comprises a gene detection element. A gene detection method in which a gene amplification operation and a gene detection operation are conducted in a sealed state using a gene detection device comprising a sealable reaction chamber and a sealable detection chamber, having a partition member which is positioned between the reaction chamber and the detection chamber and which separates the reaction chamber from the detection chamber, with test sample and gene amplification reaction solution being packed in said reaction chamber and a gene-binding agent capable of binding with a gene and generating a detectable signal being packed in said detection chamber. (1) At least the reaction chamber is imparted with conditions capable of amplifying the gene to be detected; (2) following the operation of (1), the partition member is unsealed while maintaining the gene detection tool in a sealed state; (3) through a passage created in the partition member by the unsealing, the solution in the reaction chamber is brought into contact with the gene-binding agent capable of binding the gene in the detection chamber and generating a detectable signal; and (4) the signal obtained is detected. A gene detection kit comprising the gene detection tool, a gene amplification reaction solution or gene amplification reagent, and a gene-binding agent capable of binding a gene and generating a detectable signal.
摘要翻译：一种基因检测工具，其包括可密封的反应室和可密封的检测室以及位于所述反应室和所述检测室之间的分隔部件，其分离所述反应室和所述检测室。分隔件能够开封; 反应室由至少两个能够通过相互配合设置在其中的开启装置形成密封反应室的构件组成; 并且检测室或反应室包括基因检测元件。一种基因检测方法，其中使用包括可密封反应室和可密封检测室的基因检测装置在密封状态下进行基因扩增操作和基因检测操作，所述基因检测装置具有位于反应室和反应室之间的分隔部件检测室，并且将反应室与检测室分离，将测试样品和基因扩增反应溶液包装在所述反应室中，以及能够与基因结合并产生可检测信号的基因结合剂，所述基因结合剂包装在所述检测室。（1）至少使反应室具有能够扩增待检测基因的条件; （2）在（1）的操作之后，在保持基因检测工具处于密封状态的同时将分隔构件开封; （3）通过开封在分隔构件中产生的通道，使反应室中的溶液与能够结合检测室中的基因并产生可检测信号的基因结合剂接触; 和（4）检测获得的信号。一种基因检测试剂盒，其包括基因检测工具，基因扩增反应溶液或基因扩增试剂，以及能够结合基因并产生可检测信号的基因结合剂。

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