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    • 10. 发明公开
    • Microchip for analysis, analysis system having the same, and analysis method
    • Vorrichtung,Verfahren und System zur Analyze mittels Microchips
    • EP1593968A2
    • 2005-11-09
    • EP05252674.6
    • 2005-04-28
    • SEIKO INSTRUMENTS INC.
    • Yamamoto, MinaoShinogi, Masataka
    • G01N35/00B01L3/00B01L9/00G01N33/543
    • B82Y30/00B01L3/5027B01L3/502715B01L9/527B01L2200/0684B01L2200/10B01L2200/141B01L2300/0816B01L2300/1805B01L2400/0487B01L2400/0655
    • It is a problem to provide a microchip for analysis which is miniaturized, is disposable for ease to use, and does not have a risk of contamination in flow channels. On a substrate 2, a first flow channel having a buffer solution supply channel 6 and a reaction bath section 3, a second flow channel having a sample solution supply channel 5 and a waste solution channel 7, and a connecting channel 9 connecting both the flow channels upstream of the reaction bath section 3 are provided. The waste solution channel 7 and the reaction bath section 3 are connected to a waste solution tank 8 and a pump 11. After a ligand is modified in the reaction bath section 3, a sample solution is allowed to flow in the sample solution supply channel 5 and the waste solution channel 7, and a buffer solution is allowed to flow in the buffer solution supply channel 6, the connecting channel 9 and the waste solution channel 7, subsequently to flow in the buffer solution supply channel 6 and the reaction bath section 3. Then, a temperature adjusting section 13 and the pump 11 are set such that a desired temperature of a fluid and a flow rate are obtained. Subsequently, the sample solution is supplied to the sample solution supply channel 5, the connecting channel 9, and the reaction bath section 3, a binding reaction is brought between the ligand and an analyte in the sample solution, and the state of binding is detected.
    • 提供微型化的微芯片是一个问题,其微型化,一次性易于使用,并且不具有流动通道污染的风险。 在基板2上,具有缓冲溶液供给流路6和反应槽部3的第一流路,具有试样溶液供给流路5和废液流路7的第二流路,以及连接流路 提供了反应浴部分3上游的通道。 废液通道7和反应槽部分3连接到废溶液罐8和泵11.在反应槽部分3中对配体进行改性后,允许样品溶液在样品溶液供应通道5中流动 和废液溶液通道7,并且缓冲溶液在缓冲溶液供给通道6,连接通道9和废液通道7中流动,随后流入缓冲溶液供应通道6和反应槽部分3 然后,设定温度调节部13和泵11,使得获得所需的流体温度和流量。 随后,将样品溶液供给到样品溶液供给通道5,连接通道9和反应槽部3,使配体与样品溶液中的分析物结合反应,检测结合状态 。