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1. 发明公开

EP2495334A4 METHOD FOR DETECTION OF TARGET NUCLEIC ACID 有权
标题翻译：检测方法的靶核酸的
公开(公告)号：EP2495334A4
公开(公告)日：2013-08-21
申请号：EP10826718
申请日：2010-10-26
申请人： NGK INSULATORS LTD
发明人： NIWA KOUSUKE , HIROTA TOSHIKAZU , KAWASE MITSUO
IPC分类号： C12Q1/68 , C12M1/00 , C12N15/09 , G01N33/53 , G01N33/543 , G01N37/00
CPC分类号： C12Q1/689 , C12Q1/6858 , C12Q2600/158 , C12Q2531/107 , C12Q2537/143 , C12Q2563/107 , C12Q2565/514
摘要： An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer 30 comprising an identification sequence 32 complementary to a target sequence 12 in a target nucleic acid and a tag addition sequence 36, and a second primer 40 having a label 42 are prepared. The first primer 30 and the second primer 40 are used for the target nucleic acid 10 in a sample to amplify a chimeric DNA 60 having a tag sequence 66 and the label 42. The chimeric DNA 60 is hybridized with a detection probe 104 on a solid phase 100 to obtain signal intensity information based on the label 42, and the target nucleic acid 10 is detected based on the signal intensity information.

2. 发明公开

EP1367122A4 HIGHLY SENSITIVE NUCLEIC ACID HYBRIDIZATION METHOD AND GENE ANALYSIS METHOD BY USING THE SAME 审中-公开
标题翻译： HIGH SENSIBLES杂交方法对于核酸和GENANALYSEVERFAHREN BY同样的使用
公开(公告)号：EP1367122A4
公开(公告)日：2006-04-26
申请号：EP02701660
申请日：2002-03-01
申请人： NGK INSULATORS LTD
发明人： KAWASE MITSUO , YAMADA KAZUNARI , MIYAZAKI ISAO , MATSUDA CHIHO
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6827 , C12Q2521/301 , C12Q2527/101
摘要： A nucleic acid hybridization method involving an annealing step wherein a first single-stranded nucleic acid fragment having been immobilized on the surface of an immobilization material and a second single-stranded nucleic acid fragment having been fluorescence- or radioisotope-labeled are prepared and a complementary double-strand of the first single-stranded nucleic acid fragment with the second single-stranded nucleic acid fragment is formed, and an enzyme treatment step. In the annealing step, the complementary double strand is formed under a temperature gradient from a high temperature region to a low temperature region. In the enzyme treatment stage, endonuclease is added and thus a non-complementary nucleic acid moiety contained in the complementary double strand is recognized and excised. Hybridization by this method ensures a high assay sensitivity with a convenient operation.

3. 发明公开

EP2453022A4 METHOD FOR DETECTION OR ANALYSIS OF TARGET SEQUENCE IN GENOMIC DNA 审中-公开
标题翻译：方法检测和靶序列的基因组DNA分析
公开(公告)号：EP2453022A4
公开(公告)日：2013-09-18
申请号：EP10797207
申请日：2010-07-09
申请人： NGK INSULATORS LTD
发明人： KAWASE MITSUO , HIROTA TOSHIKAZU , NIWA KOUSUKE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6837 , C12Q2565/519 , C12Q2561/125

4. 发明公开

EP1726648A4 DNA ARRAY AND METHOD OF DETECTING SINGLE NUCLEOTIDE POLYMORPHISM 审中-公开
标题翻译： DNA阵列和方法用于检测的单核苷酸多态性
公开(公告)号：EP1726648A4
公开(公告)日：2008-04-09
申请号：EP05721539
申请日：2005-03-18
申请人： TOYO BOSEKI , NGK INSULATORS LTD
发明人： KAWASE MITSUO , YOSHIDA YASUKO , YAMADA KAZUNARI , TAKARADA YUTAKA , HASHIMOTO KOUZO
IPC分类号： C12Q1/68 , C12N15/09 , G01N37/00
CPC分类号： C12Q1/6827 , C12Q2565/501 , C12Q2537/143 , C12Q2525/204
摘要： A DNA array for detecting a single nucleotide polymorphism (SNP) in a gene having first probe spots, which comprise one or more spots wherein a sequence part hybridizable with a gene polynucleotide is exactly complementary to a first polymorphism mode of the gene, and second probe spots, which comprise one or more spots wherein the sequence part is exactly complementary to a second polymorphism mode of the gene, on a solid support. The probe spots constituting the first probe spots and the second probe spots differ in length from spot to spot. This DNA array makes it possible to more exactly detect an SNP.

5. 发明公开

EP2762562A4 METHOD FOR DETECTING TARGET NUCLEIC ACID 有权
标题翻译：检测目标核酸的方法
公开(公告)号：EP2762562A4
公开(公告)日：2015-05-06
申请号：EP12832061
申请日：2012-09-14
申请人： NGK INSULATORS LTD
发明人： NIWA KOUSUKE , HIROTA TOSHIKAZU , KAWASE MITSUO
IPC分类号： C12N15/09 , C12Q1/68 , G01N33/543
CPC分类号： C12Q1/6837 , C12Q1/6844 , C12Q2525/161 , C12Q2565/519
摘要： The disclosure of the present description provides a method for detecting a target nucleic acid, whereby probe hybridization can be accomplished efficiently. To that end, a target nucleic acid is amplified using a first primer having a tag sequence complementary to a detection probe pre-associated with the target nucleic acid and a first recognition sequence that recognizes a first base sequence in the target nucleic acid and also having a linking site capable of inhibiting or arresting a DNA polymerase reaction disposed between the tag sequence and the first recognition sequence, and a second primer having a second recognition sequence that recognizes a second base sequence in the target nucleic acid, the amplified fragment is brought into contact with a detection probe so as to allow hybridization, and the hybridization product is detected.
摘要翻译：本说明书的公开内容提供了用于检测靶核酸的方法，由此可以有效地实现探针杂交。为此目的，使用具有与靶核酸预先相关联的检测探针互补的标签序列的第一引物和识别靶核酸中的第一碱基序列的第一识别序列扩增靶核酸，并且还具有能够抑制或阻止设置在标记序列和第一识别序列之间的DNA聚合酶反应的连接位点和具有识别靶核酸中的第二碱基序列的第二识别序列的第二引物，将扩增的片段置于与检测探针接触以允许杂交，并检测杂交产物。

6. 发明公开

EP0821963A4 ANTICANCER SUBSTANCE SUPPRESSING CANCEROUS METASTASIS 失效
标题翻译：癌转移抑制ANTI抗癌剂
公开(公告)号：EP0821963A4
公开(公告)日：1999-07-14
申请号：EP96901963
申请日：1996-02-08
申请人： NGK INSULATORS LTD , BISEIKEN CO LTD
发明人： HASEGAWA TAKAAKI , MIYAMOTO KENICHI , KAWASE MITSUO , YOSHIDA YASUKO , INUKAI TADAHIKO
IPC分类号： A61K31/726 , A61P35/00 , C07H7/027 , A61K31/70
CPC分类号： A61K31/7012 , A61K31/726 , C07H7/027
摘要： The present invention is aimed at providing an anticancer agent which can inhibit the metastasis of cancer cells when used alone. In addition, the present invention is also aimed at providing an anticancer agent which can inhibit the metastasis of cancer cells while causing a small side effect and very small toxicity even for an extended time of administration. The cancer metastasis-inhibiting anticancer agent according to the present invention is characterized by containing sialic acid, its salt, a polymer of sialic acid or a salt of the polymer as an effective ingredient.

7. 发明公开

EP0778026A4 REMEDY FOR ENDOTOXINEMIA AND MULTIPLE ORGAN FAILURE INDUCED THEREBY 失效
标题翻译：对内毒素药物并由此引发多器官功能衰竭
公开(公告)号：EP0778026A4
公开(公告)日：1998-08-12
申请号：EP95939419
申请日：1995-12-12
申请人： NGK INSULATORS LTD , BISEIKEN CO LTD
发明人： OHTA MICHIO , HASEGAWA TAKAAKI , NADAI MASAYUKI , YOSHIDA YASUKO , KAWASE MITSUO , INUKAI TADAHIKO
IPC分类号： A61K31/7012 , A61K31/715 , A61K31/726 , A61P31/00 , A61P43/00 , A61K31/70 , C07H7/027
CPC分类号： A61K31/726 , A61K31/7012 , A61K31/715 , Y10S514/921
摘要： A remedy for endotoxinemia and multiple organ failure induced thereby, containing sialic acid, its salt, sialic acid polymer, or its salt as the active ingredient. It is highly efficacious against death from shock and organ failure induced by endotoxin and is highly safe.

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