专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP2927238B1 Methods for LATE amplification and sequencing of a nucleic acid 有权
标题翻译：用于核酸的LATE扩增和测序的方法
公开(公告)号：EP2927238B1
公开(公告)日：2018-01-17
申请号：EP14195859.5
申请日：2005-10-17
申请人： BRANDEIS UNIVERSITY
发明人： Wangh, Lawrence J. , Rice, John , Pierce, Kenneth , Reis, Arthur , Hartshorn, Cristina , Sanchez, J. Aquiles , Salk, Jesse
IPC分类号： C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6851 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107 , C12Q2561/113
摘要： Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labelled primers and probes improves reproducibility and quantification. Low-temperature homogenous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utlilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit "single-tube" processing.

2. 发明公开

EP2855707A1 METHOD FOR ACCURATE SEQUENCING OF DNA 有权
标题翻译： VERFAHREN ZUR GENAUEN DNA-SEQUENZIERUNG
公开(公告)号：EP2855707A1
公开(公告)日：2015-04-08
申请号：EP13797483.8
申请日：2013-05-28
申请人： Board of Regents, The University of Texas System
发明人： OTWINOWSKI, Zbyszek , BOREK, Dominika
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q2525/191 , C12Q2531/107
摘要： DNA is sequenced by (a) independently sequencing first and second strands of a dsDNA to obtain corresponding first and second sequences; and (b) combining the first and second sequences to generate a consensus sequence of the dsDNA. By independently sequencing first and second strands the error probability of the consensus sequence approximates a multiplication of those of the first and second sequences.
摘要翻译：通过（a）独立测序dsDNA的第一和第二链来测序DNA以获得相应的第一和第二序列; 和（b）组合第一和第二序列以产生dsDNA的共有序列。通过对第一和第二链进行独立测序，共有序列的误差概率近似于第一和第二序列的错误概率。

3. 发明授权

EP1794319B1 METHOD FOR PROVIDING DNA FRAGMENTS DERIVED FROM AN ARCHIVED SAMPLE 有权
标题翻译： METHOD FOR PROVIDING DNA片段已封存的样品
公开(公告)号：EP1794319B1
公开(公告)日：2014-08-13
申请号：EP05802610.5
申请日：2005-09-30
申请人： Epigenomics AG
发明人： BALLHAUSE, Matthias , BERLIN, Kurt , DIETRICH, Dimo , KLUTH, Antje , SCHUSTER, Matthias , WAGNER, Ute , WASSERKORT, Reinhold , ZIEBARTH, Heike
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q1/6858 , C12Q1/686 , C12Q2523/125 , C12Q2527/137 , C12Q2527/149 , C12Q2531/107
摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.

4. 发明授权

EP2470669B1 METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 有权转让
标题翻译：方法多核苷酸的选择和扩增
公开(公告)号：EP2470669B1
公开(公告)日：2014-06-18
申请号：EP09848830.7
申请日：2009-08-25
申请人： Illumina, Inc.
发明人： SABOT, Andrea , RIGATTI, Roberto , SHEN, Min-jui, Richard
IPC分类号： C12Q1/68
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

5. 发明公开

EP2614157A4 COMPOSITIONS AND METHODS FOR NUCLEIC ACID BASED DIAGNOSTIC ASSAYS FOR VARIABLE SEQUENCE TARGETS 审中-公开
标题翻译：基于NUCLEIC测定为了具体目标变量序列组合物和诊断方法
公开(公告)号：EP2614157A4
公开(公告)日：2014-03-26
申请号：EP11824098
申请日：2011-09-08
申请人： UNIV BRANDEIS
发明人： PIERCE KENNETH E , RICE JOHN E , WANGH LAWRENCE J
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/6858 , C12Q1/686 , C12Q1/6888 , C12Q1/689 , C12Q1/6895 , C12Q1/70 , C12Q2531/107 , C12Q2537/143 , C12Q2561/113

6. 发明公开

EP2703501A1 Reagents and Methods for PCR 审中-公开
标题翻译： Reagenzien und VerfahrenfürPCR
公开(公告)号：EP2703501A1
公开(公告)日：2014-03-05
申请号：EP13195156.8
申请日：2010-03-11
申请人： BRANDEIS UNIVERSITY
发明人： Wangh, Lawrence J. , Rice, John , Rice, Nicholas , Jia, Yanwei
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107
摘要： Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least 32°C, and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified olgonucleotide being capable of binding to the 5' exonuclease domains of DNA polymerases and, when included in a PCR or other primer-dependent DNA amplification reaction at a concentration, generally not more than 2000 nM, that is effective for at least one of the functions of suppressing mispriming, increasing polymerase selectivity against 3' terminal mismatches, increasing polymerase selectivity against AT-rich 3' ends, reducing scatter among replicates, suppressing polymerase 5' exonuclease activity, and inhibiting polymerase activity; as well as amplification reaction mixtures containing such modified double-stranded oligonucleotides, and amplification reactions, amplification assays and kits that include such modified double-stranded oligonucleotides.
摘要翻译：修饰的双链寡核苷酸在其每条链上具有长度为6-50个核苷酸的长度为6-50个核苷酸的末端区域，其熔解温度Tm至少为32℃，并且包括2-4个修饰基团共价连接到不同的末端区域，优选连接到末端核苷酸，所述修饰基团是不具有非平面的庞大部分的多环取代基，所述修饰的寡核苷酸能够结合DNA聚合酶的5'核酸外切酶结构域，当包含在浓度通常不超过2000nM的PCR或其他引物依赖性DNA扩增反应中时，对于抑制错配的功能中的至少一个功能是有效的，增加聚合酶对3'末端错配的选择性，增加聚合酶选择性抗AT丰富的3'末端，减少重复之间的分散，抑制聚合酶5'核酸外切酶活性，抑制聚合酶活性; 以及含有这种修饰的双链寡核苷酸的扩增反应混合物，以及包括这种修饰的双链寡核苷酸的扩增反应，扩增测定和试剂盒。

7. 发明公开

EP2464737A4 SINGLE PROBE, MULTIPLE TEMPERATURE, NUCLEIC ACID DETECTION METHODS, KITS, AND COMPOSITIONS 审中-公开
标题翻译： EINZELSONDEN-UND MEHRTEMPERATURVERFAHREN，KITS UND ZUSAMMENSETZUNGEN ZURNUKLEINSÄUREERKENNUNG
公开(公告)号：EP2464737A4
公开(公告)日：2013-04-17
申请号：EP10808708
申请日：2010-08-11
申请人： UNIV BRANDEIS
发明人： REIS ARTHUR H JR , WANGH LAWRENCE J , PIERCE KENNETH
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6844 , C12Q1/6858 , C12Q1/689 , C12Q2600/156 , C12Q2600/16 , C12Q2527/107 , C12Q2531/107 , C12Q2565/1015

8. 发明授权

EP2046989B1 SPECIALIZED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION AND DETECTION 有权
标题翻译： SPECIALIZED寡核苷酸及其USE IN核酸扩增和检测
公开(公告)号：EP2046989B1
公开(公告)日：2013-03-20
申请号：EP07796898.0
申请日：2007-07-17
申请人： BRANDEIS UNIVERSITY
发明人： WANGH, Lawrence, J.
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/00 , C07H21/00
CPC分类号： C12Q1/686 , C12Q2531/107 , C12Q2527/143 , C12Q2525/125 , C12Q2561/101

9. 发明授权

EP2143805B1 Asymmetric PCR amplification 有权
标题翻译：不对称PCR扩增
公开(公告)号：EP2143805B1
公开(公告)日：2012-12-26
申请号：EP04797361.5
申请日：2004-11-22
申请人： CapitalBio Corporation , Tsinghua University
发明人： ZHANG, Zhiwei 18 Life Science Parkway , WANG, Can 18 Life Science Parkway , ZHU, Lingxiang 18 Life Science Parkway , ZHANG, Qiong 18 Life Science Parkway , CHANG, Jing 18 Life Science Parkway
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2531/107 , C12Q2525/155 , C12Q2525/15

10. 发明公开

EP2341151A1 Methods for determining sequence variants using ultra-deep sequencing 有权
标题翻译： Verfahren zur Bestimmung von Sequenzvarianten mittels Ultra-deep-Sequenzierung
公开(公告)号：EP2341151A1
公开(公告)日：2011-07-06
申请号：EP10179666.2
申请日：2006-04-12
申请人： 454 Life Sciences Corporation
发明人： Leamon, John, Harris , Lee, William, Lun , Simons, Jan, Fredrick , Desany, Brian , Ronan, Mike, Todd , Drake, James , Lohman, Kenton , Egholm, Michael , Rothberg, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2565/301 , C12Q2531/107 , C12Q2531/101 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114
摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.
摘要翻译：所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。本公开中提供的技术允许一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式