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1. 发明授权

EP3052656B1 METHODS OF DETERMINING POLYMORPHISMS 有权
公开(公告)号：EP3052656B1
公开(公告)日：2018-12-12
申请号：EP14848135.1
申请日：2014-09-30
申请人： President and Fellows of Harvard College
发明人： MACLEOD, Iain J. , ROWLEY, Christopher F. , ESSEX, Max
IPC分类号： C12Q1/6827 , C12Q1/70
CPC分类号： C12Q1/6827 , C12Q1/703 , C12Q2600/106 , C12Q2600/156 , C12Q2525/15 , C12Q2525/161 , C12Q2525/179 , C12Q2525/191
摘要： Methods and compositions for determining the presence of a polymorphism at a target nucleotide position in a plurality of target nucleic acid sequences is provided.

2. 发明授权

EP2765424B1 METHOD FOR ANALYZING BIOMOLECULES 有权转让
公开(公告)号：EP2765424B1
公开(公告)日：2018-12-05
申请号：EP12838806.3
申请日：2012-10-04
申请人： Hitachi High-Technologies Corporation
发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
IPC分类号： G01N33/553 , C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6804 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N33/54326 , G01N33/54346 , G01N33/54393 , G01N33/587 , G01N2021/6421 , C12Q2525/161 , C12Q2545/114 , C12Q2563/107 , C12Q2563/143 , C12Q2563/149 , C12Q2565/514 , C12Q2563/119
摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.

3. 发明授权

EP2809797B1 DETECTION OF TARGET NUCLEIC ACID SEQUENCE BY PTO CLEAVAGE AND EXTENSION-DEPENDENT SIGNALING OLIGONUCLEOTIDE HYBRIDIZATION ASSAY 有权
公开(公告)号：EP2809797B1
公开(公告)日：2018-11-28
申请号：EP12867064.3
申请日：2012-07-03
申请人： Seegene, Inc.
发明人： CHUN, Joug Yoon , LEE, Young Jo
IPC分类号： C12Q1/68 , C12Q1/34 , C12N15/11
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q2521/319 , C12Q2525/131 , C12Q2525/161 , C12Q2525/186 , C12Q2533/101 , C12Q2563/173 , C12Q2565/1015 , C12Q2565/514
摘要： The present invention relates to the detection of a target nucleic acid sequence by a PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) assay. The present invention does not use probes to be hybridized with target nucleic acid sequences for providing target signals. Interestingly, the present invention uses probes (signaling oligonucleotides) to be hybridized with the extended strand formed in a target-dependent manner in which the extended strand is synthesized using the CTO artificially selected as templates.

4. 发明授权

EP1196630B2 DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS 有权
公开(公告)号：EP1196630B2
公开(公告)日：2018-10-17
申请号：EP00926204.9
申请日：2000-04-20
申请人： Illumina, Inc.
发明人： GUNDERSON, Kevin , STUELPNAGEL, John, R. , CHEE, Mark, S.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , C12Q1/6813 , C12Q1/6834 , C12Q1/6869 , C40B40/06 , C12Q2565/301 , C12Q2533/101 , C12Q2521/543 , C12Q2533/107 , C12Q2525/161 , C12Q2561/125 , C12Q2565/518 , C12Q2565/607 , C12Q2537/143
摘要： The present invention provides a method of sequencing a plurality of target nucleic acids each comprising a first domain and an adjacent second domain, in which a plurality of hybridization complexes each comprising a target sequence and a sequencing primer hybridize to the first domain of said target sequence, the hybridization complexes are attached to microspheres,

5. 发明公开

EP3377651A1 A METHOD AND A KIT FOR NUCLEIC ACID DETECTION 有权
公开(公告)号：EP3377651A1
公开(公告)日：2018-09-26
申请号：EP16822268.5
申请日：2016-11-16
申请人： Fondazione Istituto Italiano di Tecnologia
发明人： POMPA, Pier Paolo , VALENTINI, Paola
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/682 , C12Q2521/301 , C12Q2525/131 , C12Q2525/161
摘要： The invention relates to a novel technique for detecting DNA or RNA target nucleic acids, and to a suitable kit for its implementation. The new technique described is based on isothermal amplification of nucleic acids mediated by cooperative hybridization, designated as linear CHAMP.

6. 发明公开

EP3371309A1 COMBINATORIAL SETS OF NUCLEIC ACID BARCODES FOR ANALYSIS OF NUCLEIC ACIDS ASSOCIATED WITH SINGLE CELLS 有权
公开(公告)号：EP3371309A1
公开(公告)日：2018-09-12
申请号：EP16801644.2
申请日：2016-11-04
申请人： Atreca, Inc.
发明人： WITHEY, Gary
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1096 , C12N15/1065 , C12Q1/6806 , C12Q2525/131 , C12Q2525/155 , C12Q2525/191 , C12Q2563/149 , C12Q2563/185 , C12Q2525/161 , C12Q2535/122 , C12Q2563/159 , C12Q2563/179 , C12Q2565/514 , C12Q2525/173 , C12Q2523/313 , C12Q2523/319
摘要： Provided herein are methods of identifying the origin of a nucleic acid sample. The methods include forming a reaction mixture comprising a nucleic acid sample comprising nucleic acid molecules from a single cell and a set of barcodes, incorporating the set of barcodes into the nucleic acid molecules of the sample, and identifying the set of barcodes incorporated into the nucleic acid molecules of the single cell thereby identifying the origin of the nucleic acid sample.

7. 发明公开

EP3368668A1 METHOD AND APPARATUS FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION 有权
公开(公告)号：EP3368668A1
公开(公告)日：2018-09-05
申请号：EP16860845.3
申请日：2016-10-27
申请人： Silicon Valley Scientific, Inc.
发明人： JOVANOVICH, Stevan, B. , WAGNER, Peter
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1003 , C12N15/1017 , C12N15/1093 , C12Q1/6806 , C12Q2525/191 , C12Q2535/122 , C12Q2563/179 , C12Q2525/161 , C12Q2525/173 , C12Q2563/149 , C12Q2563/159 , C12Q2565/514 , C12Q2521/507
摘要： A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.

8. 发明公开

EP3360972A1 SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS 有权
公开(公告)号：EP3360972A1
公开(公告)日：2018-08-15
申请号：EP18158658.7
申请日：2009-01-15
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844 , C12Q2565/537 , C12Q2565/519 , C12Q2565/501 , C12Q2531/101 , C12Q2525/161 , C12Q2525/155
摘要： The present invention relates to a method for preparing sample nucleic acid complements, which comprises: a. preparing a mixture comprising sample nucleic acid and a collection of solid supports under conditions in which a single molecule of the sample nucleic acid hybridizes to a nucleic acid molecule on a solid support in the collection, wherein: the nucleic acid molecules on each solid support in the collection comprise a single-stranded solid phase nucleic acid species including a primer sequence, an identifier sequence and a probe sequence orientated 5' - (primer sequence) - (identifier sequence) - (probe sequence) - 3'; the probe sequence of each solid phase nucleic acid species on a solid support in the collection hybridizes to a subsequence of the sample nucleic acid sequence when the probe sequence is complementary to a nucleotide subsequence in the sample nucleic acid sequence; the solid phase nucleic acid species of each solid support in the collection share a common probe sequence or the solid phase nucleic acid species do not share a common probe sequence with other solid phase nucleic acid species on the solid support; the solid phase nucleic acid species on each solid support in the collection share a common identifier sequence; and the sample nucleic acid is single-stranded nucleic acid; and b. contacting the mixture with extension reagents under conditions in which solid phase nucleic acid species hybridized to sample nucleic acid is extended by the reagents; whereby sample nucleic acid complements comprising the shared, unique identifier sequence of a solid support in the collection and comprising subsequences of the sample nucleic acid sequence are prepared.

9. 发明公开

EP3250703A4 LIGATION ASSAYS IN LIQUID PHASE 有权转让
公开(公告)号：EP3250703A4
公开(公告)日：2018-08-01
申请号：EP16744000
申请日：2016-01-26
申请人： BIOSPYDER TECH INC
发明人： STEVENS ANTHONY , SELIGMANN BRUCE , YEAKLEY JOANNE M , MCCOMB JOEL
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2521/325 , C12Q2525/155 , C12Q2525/161 , C12Q2525/185 , C12Q2525/186 , C12Q2533/101 , C12Q2533/107 , C12Q2535/122 , C12Q2565/629 , C12Q2521/307 , C12Q2561/125
摘要： Ligation assays in liquid phase for detecting nucleic acid sequences.

10. 发明公开

EP3256624A4 MATERIALS AND METHODS TO ANALYZE RNA ISOFORMS IN TRANSCRIPTOMES 无效
公开(公告)号：EP3256624A4
公开(公告)日：2018-07-25
申请号：EP16749926
申请日：2016-02-12
申请人： VACCINE RES INSTITUTE OF SAN DIEGO
发明人： WELSH JOHN
IPC分类号： C40B20/04 , C07H21/00 , C40B30/04 , C40B40/06 , C40B50/06 , C40B70/00
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6832 , C12Q2521/101 , C12Q2521/107 , C12Q2525/161 , C12Q2525/173 , C12Q2525/179 , C12Q2525/185 , C12Q2531/101 , C12Q2533/101 , C12Q2535/122 , C12Q2563/159
摘要： The present invention includes, but is not limited to, methods, assays, and compositions for preparing libraries of nucleic acid molecules from biological samples, and for detecting and measuring the abundances of nucleic acid molecules, including RNA and DNA. The methods, assays, and compositions of the present invention provide in whole or in part for the detecting and measuring the abundances of nucleic acid molecules, and particularly but not limited to those nucleic acids that have structures that cannot be determined reliably by routine alignment to a reference sequence or concatenation of smaller sequences by matching homologous ends.

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