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1. 发明授权

EP2872628B1 5´ PROTECTION DEPENDENT AMPLIFICATION 有权
标题翻译： 5保护依赖性扩增
公开(公告)号：EP2872628B1
公开(公告)日：2018-04-04
申请号：EP13736552.4
申请日：2013-07-10
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , GABLER, Irmlind , PAUL, Lukas
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1096 , C12P19/34 , C12Q1/6844 , C12Q1/6858 , C12Q2521/107
摘要： The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5' protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5' protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, and modifying the extension product of said nucleic acids without 5' protecting group either on the 5' end of the template strand or on the 3' end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified in step c), thereby specifically generating a labelled nucleic acid complementary to an RNA at least originally comprising a 5' protecting group.

2. 发明公开

EP2681544A1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 失效
标题翻译： ARRAY MADE OUT节点指向官能化电极
公开(公告)号：EP2681544A1
公开(公告)日：2014-01-08
申请号：EP12707463.1
申请日：2012-02-27
申请人： Lexogen GmbH
发明人： REDA, Torsten , HAGLMÜLLER, Jakob , SCHITTER, Georg , SEITZ, Alexander
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6837 , G01N27/3278
摘要： The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.

3. 发明授权

EP1954829B1 POLYNUCLEOTIDE AMPLIFICATION 有权转让
标题翻译：多核苷酸扩增
公开(公告)号：EP1954829B1
公开(公告)日：2010-06-09
申请号：EP06827949.6
申请日：2006-11-29
申请人： Lexogen GmbH
发明人： SEITZ, Alexander
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6844 , C12Q1/6853 , C12Q2539/113 , C12Q2521/131 , C12Q2525/179 , C12Q2521/107
摘要： The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3' end of the transcribed cDNA with a polynucleotide tail after the 3' end, c) amplification of the cDNA using a pair of primers, wherein a first (3') primer is specific for the 5' end of the cDNA and a second (51) primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3' polynucleotide tail of the cDNA.
摘要翻译：本发明提供了扩增样品中多核苷酸分子库的方法，其特征在于以下步骤：a）获得样品或RNA并逆转录整个RNA分子，从而产生全长cDNA或获得全长cDNA样品， b）在3'末端后用多核苷酸尾拖尾转录的cDNA的3'末端，c）用一对引物扩增cDNA，其中第一（3'）引物对于 cDNA和第二（51）引物对于多核苷酸尾部的上游部分和cDNA的3'多核苷酸尾部的下一个1至10个核苷酸是特异性的。

4. 发明公开

EP3894595A1 NUCLEIC ACID AMPLIFICATION AND IDENTIFICATION METHOD 审中-公开
公开(公告)号：EP3894595A1
公开(公告)日：2021-10-20
申请号：EP19816800.7
申请日：2019-12-13
申请人： Lexogen GmbH
发明人： GÖPEL, Yvonne , MOLL, Pamela , REDA, Torsten , SEITZ, Alexander
IPC分类号： C12Q1/6855

5. 发明授权

EP2531610B1 COMPLEXITIY REDUCTION METHOD 有权
标题翻译：完全简化法
公开(公告)号：EP2531610B1
公开(公告)日：2018-03-21
申请号：EP11702031.3
申请日：2011-02-02
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , HAGLMÜLLER, Jakob , REDA, Torsten
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6869 , C12Q2523/301 , C12Q2537/143 , C12Q2525/191
摘要： The present invention relates to a method for the reduction of the complexity of nucleic acid pool(s), comprising €¢ providing a sample with one or more nucleic acid molecules, €¢ cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments, €¢ amplifying one or more fragments of said nucleic acid molecules, wherein the one or more fragments constitute at least a fraction of all fragments of the nucleic acid molecules, and wherein the amplified or amplifying one or more fragments of said fraction are divided into different subpools, and wherein the fragments of each subpool comprise a common nucleic acid feature.

6. 发明授权

EP2681545B1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 失效
标题翻译： BETWEEN功能化电极网格点的BIOSENSORARRAY做出来的
公开(公告)号：EP2681545B1
公开(公告)日：2015-04-29
申请号：EP12708646.0
申请日：2012-02-27
申请人： Lexogen GmbH
发明人： REDA, Torsten , SEITZ, Alexander , HOLLÄNDER, Igor
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/6837 , B82Y15/00 , G01N27/3278 , G01N33/5438 , G01N33/553

7. 发明公开

EP4222284A1 METHOD AND MEANS FOR GENERATING TRANSCRIBED NUCLEIC ACIDS 审中-实审
公开(公告)号：EP4222284A1
公开(公告)日：2023-08-09
申请号：EP21783301.1
申请日：2021-10-01
申请人： Lexogen GmbH
发明人： SURVILA, Mantas , MOLL, Pamela , SEITZ, Alexander
IPC分类号： C12Q1/6865

8. 发明公开

EP3978626A1 METHOD AND MEANS FOR GENERATING TRANSCRIBED NUCLEIC ACIDS 审中-公开
公开(公告)号：EP3978626A1
公开(公告)日：2022-04-06
申请号：EP20199844.0
申请日：2020-10-02
申请人： Lexogen GmbH
发明人： SURVILA, Mantas , MOLL, Pamela , SEITZ, Alexander
IPC分类号： C12Q1/6865
摘要： The present invention relates to a method of generating transcribed nucleic acids, comprising the steps of providing a nucleic acid template, hybridizing an oligonucleotide probe to the nucleic acid template, wherein said oligonucleotide probe comprises a complementary part that hybridizes to the nucleic acid template and a non-complementary part in 5' direction of the complementary part that does not hybridize to the nucleic acid template and comprises a sequence of a transcription promoter, hydrolysing a 3' part of the nucleic acid template that is in 3' direction to a part of the nucleic acid template that hybridizes to the oligonucleotide probe and wherein said 3' part is not hybridized to the oligonucleotide probe, extending the nucleic acid template with nucleic acids complementary to the non-complementary part of the oligonucleotide probe, thereby generating a duplex of the sequence of the transcription promoter in sequence with the nucleic acid template, transcribing the nucleic acid template with a transcriptase that binds the duplex of the sequence of the transcription promoter, thereby generating the transcribed nucleic acids; and kits and nucleic acids for performing such a method.

9. 发明授权

EP2756098B1 METHOD FOR MAKING LIBRARY OF NUCLEIC ACID MOLECULES 有权
公开(公告)号：EP2756098B1
公开(公告)日：2018-06-06
申请号：EP12759455.4
申请日：2012-09-17
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , MOLL, Pamela , NAPORA, Magdalena Anna
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6816 , C12Q1/6853 , C12Q2521/107 , C12Q2525/113 , C12Q2533/107 , C12Q2521/501 , C12Q2525/161
摘要： The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3' end of said oligonucleotide stopper - said stopper may also be a primer itself - and uses and kits for performing said method.

10. 发明公开

EP2531610A1 COMPLEXITIY REDUCTION METHOD 有权
标题翻译： KOMPLEXITÄTSREDUZIERUNGSVERFAHREN
公开(公告)号：EP2531610A1
公开(公告)日：2012-12-12
申请号：EP11702031.3
申请日：2011-02-02
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , HAGLMÜLLER, Jakob , REDA, Torsten
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6869 , C12Q2523/301 , C12Q2537/143 , C12Q2525/191
摘要： The present invention relates to a method for the reduction of the complexity of nucleic acid pool(s), comprising €¢ providing a sample with one or more nucleic acid molecules, €¢ cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments, €¢ amplifying one or more fragments of said nucleic acid molecules, wherein the one or more fragments constitute at least a fraction of all fragments of the nucleic acid molecules, and wherein the amplified or amplifying one or more fragments of said fraction are divided into different subpools, and wherein the fragments of each subpool comprise a common nucleic acid feature.
摘要翻译：本发明涉及降低核酸池的复杂性的方法，其包括向样品提供一种或多种核酸分子，通过随机和/或序列独立地切割核酸分子切割步骤，从而获得核酸片段池，扩增所述核酸分子的一个或多个片段，其中所述一个或多个片段构成核酸分子的所有片段的至少一部分，并且其中扩增或扩增所述级分的一个或多个片段被分成不同的子池，并且其中每个子池的片段包含共同的核酸特征。

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