专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP2872628B1 5´ PROTECTION DEPENDENT AMPLIFICATION 有权
标题翻译： 5保护依赖性扩增
公开(公告)号：EP2872628B1
公开(公告)日：2018-04-04
申请号：EP13736552.4
申请日：2013-07-10
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , GABLER, Irmlind , PAUL, Lukas
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1096 , C12P19/34 , C12Q1/6844 , C12Q1/6858 , C12Q2521/107
摘要： The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5' protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5' protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, and modifying the extension product of said nucleic acids without 5' protecting group either on the 5' end of the template strand or on the 3' end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified in step c), thereby specifically generating a labelled nucleic acid complementary to an RNA at least originally comprising a 5' protecting group.

2. 发明公开

EP2681544A1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 失效
标题翻译： ARRAY MADE OUT节点指向官能化电极
公开(公告)号：EP2681544A1
公开(公告)日：2014-01-08
申请号：EP12707463.1
申请日：2012-02-27
申请人： Lexogen GmbH
发明人： REDA, Torsten , HAGLMÜLLER, Jakob , SCHITTER, Georg , SEITZ, Alexander
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6837 , G01N27/3278
摘要： The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.

3. 发明授权

EP1954829B1 POLYNUCLEOTIDE AMPLIFICATION 有权转让
标题翻译：多核苷酸扩增
公开(公告)号：EP1954829B1
公开(公告)日：2010-06-09
申请号：EP06827949.6
申请日：2006-11-29
申请人： Lexogen GmbH
发明人： SEITZ, Alexander
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6844 , C12Q1/6853 , C12Q2539/113 , C12Q2521/131 , C12Q2525/179 , C12Q2521/107
摘要： The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3' end of the transcribed cDNA with a polynucleotide tail after the 3' end, c) amplification of the cDNA using a pair of primers, wherein a first (3') primer is specific for the 5' end of the cDNA and a second (51) primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3' polynucleotide tail of the cDNA.
摘要翻译：本发明提供了扩增样品中多核苷酸分子库的方法，其特征在于以下步骤：a）获得样品或RNA并逆转录整个RNA分子，从而产生全长cDNA或获得全长cDNA样品， b）在3'末端后用多核苷酸尾拖尾转录的cDNA的3'末端，c）用一对引物扩增cDNA，其中第一（3'）引物对于 cDNA和第二（51）引物对于多核苷酸尾部的上游部分和cDNA的3'多核苷酸尾部的下一个1至10个核苷酸是特异性的。

4. 发明公开

EP2872628A2 5´ PROTECTION DEPENDENT AMPLIFICATION 有权
标题翻译： 5-SCHUTZABHÄNGIGEVERSTÄRKUNG
公开(公告)号：EP2872628A2
公开(公告)日：2015-05-20
申请号：EP13736552.4
申请日：2013-07-10
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , GABLER, Irmlind , PAUL, Lukas
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1096 , C12P19/34 , C12Q1/6844 , C12Q1/6858 , C12Q2521/107
摘要： The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5' protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5' protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, and modifying the extension product of said nucleic acids without 5' protecting group either on the 5' end of the template strand or on the 3' end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified in step c), thereby specifically generating a labelled nucleic acid complementary to an RNA at least originally comprising a 5' protecting group.
摘要翻译：本发明涉及从包含5'保护基团的RNA产生标记的核酸的方法，所述方法包括以下步骤：获得核酸模板链的混合物，所述混合物包含所述RNA和另外潜在的其它核酸，而没有 5'保护基团，至少一个寡核苷酸引物退火至所述RNA的模板链和潜在的其它核酸，以及延伸所述引物的模板序列，从而获得退火至其模板链的互补核酸链，或提供RNA 与退火至其模板链的互补核酸链双链体，以及在模板链的5'末端或互补链的3'末端修饰所述核酸的延伸产物，而不具有5'保护基团，或并且标记在步骤c）中未修饰的双链核酸的互补核酸，从而具体产生与至少最初包含5'保护基的RNA互补的标记核酸。

5. 发明公开

EP2756098A2 NUCLEIC ACID TRANSCRIPTION METHOD 有权
标题翻译： VERFAHREN ZURNUKLEINSÄURETRANSKRIPTION
公开(公告)号：EP2756098A2
公开(公告)日：2014-07-23
申请号：EP12759455.4
申请日：2012-09-17
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , MOLL, Pamela , NAPORA, Magdalena Anna
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6816 , C12Q1/6853 , C12Q2521/107 , C12Q2525/113 , C12Q2533/107 , C12Q2521/501 , C12Q2525/161
摘要： The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3' end of said oligonucleotide stopper - said stopper may also be a primer itself - and uses and kits for performing said method.
摘要翻译：本发明涉及产生模板核酸的扩增核酸部分的方法，包括获得所述模板核酸，将至少一种寡核苷酸引物退火至所述模板核酸，将至少一种寡核苷酸终止物退火至所述模板核酸，以模板特异性方式延长所述至少一个寡核苷酸引物直到所述延伸产物核酸到达退火的寡核苷酸终止位置，由此停止所述延伸反应，其中在所述延伸反应中所述寡核苷酸终止子不伸长，并且其中所述细长将产物核酸连接到所述寡核苷酸停止物的3'末端 - 所述塞子也可以是引物本身，并且用于和执行所述方法的试剂盒。

6. 发明公开

EP2681545A1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 失效
标题翻译： BETWEEN功能化电极网格点的BIOSENSORARRAY做出来的
公开(公告)号：EP2681545A1
公开(公告)日：2014-01-08
申请号：EP12708646.0
申请日：2012-02-27
申请人： Lexogen GmbH
发明人： REDA, Torsten , SEITZ, Alexander , HOLLÄNDER, Igor
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/6837 , B82Y15/00 , G01N27/3278 , G01N33/5438 , G01N33/553
摘要： We provide a sensor array device for the measurement of mixtures of organic compounds comprising an assembly of sensor half elements which have been functionalized through sensor compounds before the assembly. Each individual sensor of the array contains two sensor compounds which are bound at opposite sensor half elements. The molecular recognition is bi-functional. While the amount of sensor compounds increases linearly, the individual sensors increase with the second power.

7. 发明公开

EP2510114A1 RNA ANALYTICS METHOD 有权转让
标题翻译： RNA分析方法
公开(公告)号：EP2510114A1
公开(公告)日：2012-10-17
申请号：EP10788340.7
申请日：2010-12-10
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , PAUL, Lukas , van MIN, Max Jan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q2537/143 , C12Q2535/122 , C12Q2525/191 , C12Q2563/179 , C12Q2539/107
摘要： The present invention relates to a method of ordering nucleic acid molecule fragment sequences derived from a pool of potentially diverse RNA molecules comprising €¢ optionally reverse transcribing the RNA molecules to provide a pool of cDNA molecules, €¢ segregating nucleic acids from said template RNA or cDNA pool, selecting for potentially different templates with a distinctive nucleic acid feature shared by the segregated templates, thereby providing at least a first subpool of nucleic acids, €¢ optionally once or more further segregating nucleic acids from said template RNA or cDNA, selectively segregating nucleic acids with a different distinctive nucleic acid feature, thereby providing one or more further subpool(s) of nucleic acids, €¢ generating fragments of said segregated nucleic acid molecules by fragmenting or obtaining fragment copies of said segregated nucleic acid molecules, wherein the fragments of each subpool or combined subpools remain separable from fragments of other subpools or other combined subpools by physically separating the subpools or by attaching a label to the fragments of the subpools, with the label identifying a subpool, or determining a partial sequence of said segregated nucleic acid molecule and preferably aligning at least two sequences or partial sequences to a joined sequence.

8. 发明公开

EP3894595A1 NUCLEIC ACID AMPLIFICATION AND IDENTIFICATION METHOD 审中-公开
公开(公告)号：EP3894595A1
公开(公告)日：2021-10-20
申请号：EP19816800.7
申请日：2019-12-13
申请人： Lexogen GmbH
发明人： GÖPEL, Yvonne , MOLL, Pamela , REDA, Torsten , SEITZ, Alexander
IPC分类号： C12Q1/6855

9. 发明授权

EP2531610B1 COMPLEXITIY REDUCTION METHOD 有权
标题翻译：完全简化法
公开(公告)号：EP2531610B1
公开(公告)日：2018-03-21
申请号：EP11702031.3
申请日：2011-02-02
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , HAGLMÜLLER, Jakob , REDA, Torsten
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6869 , C12Q2523/301 , C12Q2537/143 , C12Q2525/191
摘要： The present invention relates to a method for the reduction of the complexity of nucleic acid pool(s), comprising €¢ providing a sample with one or more nucleic acid molecules, €¢ cutting the nucleic acid molecules by a random and/or sequence independent cutting step thereby obtaining a pool of nucleic acid fragments, €¢ amplifying one or more fragments of said nucleic acid molecules, wherein the one or more fragments constitute at least a fraction of all fragments of the nucleic acid molecules, and wherein the amplified or amplifying one or more fragments of said fraction are divided into different subpools, and wherein the fragments of each subpool comprise a common nucleic acid feature.

10. 发明授权

EP2681545B1 BIOSENSOR ARRAY FORMED BY JUNCTIONS OF FUNCTIONALIZED ELECTRODES 失效
标题翻译： BETWEEN功能化电极网格点的BIOSENSORARRAY做出来的
公开(公告)号：EP2681545B1
公开(公告)日：2015-04-29
申请号：EP12708646.0
申请日：2012-02-27
申请人： Lexogen GmbH
发明人： REDA, Torsten , SEITZ, Alexander , HOLLÄNDER, Igor
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/6837 , B82Y15/00 , G01N27/3278 , G01N33/5438 , G01N33/553

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式