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1. 发明公开

EP2756098A2 NUCLEIC ACID TRANSCRIPTION METHOD 有权
标题翻译： VERFAHREN ZURNUKLEINSÄURETRANSKRIPTION
公开(公告)号：EP2756098A2
公开(公告)日：2014-07-23
申请号：EP12759455.4
申请日：2012-09-17
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , MOLL, Pamela , NAPORA, Magdalena Anna
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6816 , C12Q1/6853 , C12Q2521/107 , C12Q2525/113 , C12Q2533/107 , C12Q2521/501 , C12Q2525/161
摘要： The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3' end of said oligonucleotide stopper - said stopper may also be a primer itself - and uses and kits for performing said method.
摘要翻译：本发明涉及产生模板核酸的扩增核酸部分的方法，包括获得所述模板核酸，将至少一种寡核苷酸引物退火至所述模板核酸，将至少一种寡核苷酸终止物退火至所述模板核酸，以模板特异性方式延长所述至少一个寡核苷酸引物直到所述延伸产物核酸到达退火的寡核苷酸终止位置，由此停止所述延伸反应，其中在所述延伸反应中所述寡核苷酸终止子不伸长，并且其中所述细长将产物核酸连接到所述寡核苷酸停止物的3'末端 - 所述塞子也可以是引物本身，并且用于和执行所述方法的试剂盒。

2. 发明公开

EP3263718A1 NUCLEIC ACID TRANSCRIPTION METHOD 审中-公开
标题翻译：核酸转录方法
公开(公告)号：EP3263718A1
公开(公告)日：2018-01-03
申请号：EP17184341.0
申请日：2012-09-17
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , MOLL, Pamela , NAPORA, Magdalena Anna
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1096 , C12Q1/6816 , C12Q1/6853 , C12Q2521/107 , C12Q2525/113 , C12Q2533/107 , C12Q2521/501 , C12Q2525/161
摘要： The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid,
annealing at least one oligonucleotide primer to said template nucleic acid,
annealing at least one oligonucleotide stopper to said template nucleic acid,
elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and
wherein the elongated product nucleic acid is ligated to the 3' end of said oligonucleotide stopper - said stopper may also be a primer itself - and uses and kits for performing said method.
摘要翻译：本发明涉及用于产生模板核酸的扩增核酸部分的方法，包括获得所述模板核酸，将至少一种寡核苷酸引物与所述模板核酸退火，将至少一种寡核苷酸终止子与所述模板核酸退火，以模板特异性方式延伸至少一个寡核苷酸引物，直到延伸产物核酸到达退火的寡核苷酸终止子的位置，由此停止延伸反应，其中在所述延伸反应中，所述寡核苷酸终止子不被拉长，并且其中细长产物核酸连接到所述寡核苷酸塞子的3'末端 - 所述塞子也可以是引物本身 - 以及用于实施所述方法的试剂盒和试剂盒。

3. 发明授权

EP2756098B1 METHOD FOR MAKING LIBRARY OF NUCLEIC ACID MOLECULES 有权
公开(公告)号：EP2756098B1
公开(公告)日：2018-06-06
申请号：EP12759455.4
申请日：2012-09-17
申请人： Lexogen GmbH
发明人： SEITZ, Alexander , MOLL, Pamela , NAPORA, Magdalena Anna
IPC分类号： C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6816 , C12Q1/6853 , C12Q2521/107 , C12Q2525/113 , C12Q2533/107 , C12Q2521/501 , C12Q2525/161
摘要： The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3' end of said oligonucleotide stopper - said stopper may also be a primer itself - and uses and kits for performing said method.

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