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1. 发明公开

EP1774471A1 ASSAY FOR DISTINGUISHING LIVE AND DEAD CELLS 审中-公开
标题翻译：分析法的区别死细胞和活
公开(公告)号：EP1774471A1
公开(公告)日：2007-04-18
申请号：EP05770251.6
申请日：2005-07-06
申请人： Cytokinetics, Inc.
发明人： FAN, Jinhong , KUTSYY, Vadim , VAISBERG, Eugeni, A.
IPC分类号： G06T7/00
CPC分类号： G06K9/00147 , G06T7/0012
摘要： ABSTRACT Image analysis methods and apparatus are used for distinguishing live and dead cells. The methods may involve segmenting an image to identify the region(s) occupied by one or more cells and determining the presence of a particular live-dead indicator feature within the region(s). In certain embodiments, the indicator feature is a cytoskeletal component such as tubulin. Prior to producing an image for analysis, cells may be treated with a marker that highlights the live-dead indicator in the image. In the case of tubulin, the marker will co-locate with tubulin and provide a signal that is captured in the image (e.g., a fluorescent emission).

2. 发明公开

EP1646926A2 CHARACTERIZING BIOLOGICAL STIMULI BY RESPONSE CURVES 审中-公开
标题翻译：生物刺激BY响应曲线表征
公开(公告)号：EP1646926A2
公开(公告)日：2006-04-19
申请号：EP04778162.0
申请日：2004-07-16
申请人： Cytokinetics, Inc.
发明人： KUTSYY, Vadim , COLEMAN, Daniel, A. , VAISBERG, Eugeni, A.
IPC分类号： G06F1/00
CPC分类号： G06K9/6274 , G06K9/00147 , G06K9/00536
摘要： A method for calculating distances between stimulus response curves (e.g., dose response curves) allows classification of stimuli, The response curves show how the phenotype of one or more cells changes in response to varying levels of the stimulus. Each “point” on the curve represents quantitative phenotype or signature for cell(s) at a particular level of stimulus (e.g., dose of a therapeutic). The signatures are multivariate phenotypic representations of the cell (s). They include various features of the cell(s) obtained by image analysis. To facilitate the comparison of stimuli, distances between points on the response curves are calculated. First, the response curves may be aligned on a coordinate representing a separate distance, r, from a common point of negative control (e.g., the point where no stimulus is applied). Integration on r may be used to compute the distance between two response curves. The distance between response curves is used to classify stimuli.

3. 发明公开

EP1362239A2 CHARACTERIZING BIOLOGICAL STIMULI BY RESPONSE CURVES 审中-公开
标题翻译：生物REIZEN由浓度曲线表征
公开(公告)号：EP1362239A2
公开(公告)日：2003-11-19
申请号：EP02742492.8
申请日：2002-02-20
申请人： Cytokinetics, Inc.
发明人： ADAMS, Cynthia, L. , OESTREICHER, Donald, R. , VAISBERG, Eugeni, A.
IPC分类号： G01N33/50
CPC分类号： G06K9/6253 , G06F19/70 , G06K9/00127
摘要： A method for generating stimulus response curves (e.g., dose response curves) shows how the phenotype of one or more cells change in response to varying levels of the stimulus. Each 'point' on the curve represents quantitative phenotype for cell(s) at a particular level of stimulus (e.g., dose of a therapeutic). The quantitative phenotypes are multivariate phenotypic representations of the cell(s). They include various features of the cell(s) obtained by image analysis. Such features often include basic parameters obtained from images (e.g., cell shape, nucleus area, Golgi texture) and/or biological characterizations derived from the basic parameters (e.g., cell cycle state, mitotic index, etc.). The stimulus response curves may be compared to allow classification of stimuli and identify subtle differences in related stimuli. To facilitate the comparison, it may be desirable to present the response curves in a principal component space.

4. 发明公开

EP1340194A1 CLASSIFYING CELLS BASED ON INFORMATION CONTAINED IN CELL IMAGES 审中-公开
标题翻译：荷兰皇家银行信托基金信贷银行KLASSIFIZIERUNG VON ZELLEN
公开(公告)号：EP1340194A1
公开(公告)日：2003-09-03
申请号：EP01968609.6
申请日：2001-09-07
申请人： Cytokinetics, Inc.
发明人： VAISBERG, Eugeni, A. , COLEMAN, Daniel, A.
IPC分类号： G06T7/60 , G06F19/00
CPC分类号： G06K9/0014 , G06T7/11 , G06T7/60 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： Image analysis methods analyze images of cells and place the cells in particular cell cycle phases based upon certain features extracted from the images. The methods can also quantify the total amount of DNA in a cell based on specific features such as fluorescence intensity from fluorescent molecules that bind to DNA. Further, the methods can characterize a cell as mitotic or interphase based on chosen parameters such as the variance in intensity observed in a cell image and/or the size of a region containing DNA. In one example, image analysis methods can classify the cell into one of the following five phases: G1, S, G2, telophase, and an early stage mitotic phase comprised of prophase, metaphase, and anaphase.
摘要翻译：图像分析方法基于从图像提取的某些特征分析细胞的图像并将细胞置于特定的细胞周期阶段。该方法还可以基于特定特征（例如来自结合DNA的荧光分子的荧光强度）来量化细胞中DNA的总量。此外，所述方法可以基于所选择的参数（例如在细胞图像中观察到的强度变化）和/或含有DNA的区域的大小来表征细胞作为有丝分裂或间期。在一个实例中，图像分析方法可以将细胞分为以下五个阶段之一：G1，S，G2，端粒酶，以及由前期，中期和后期组成的早期有丝分裂期。

5. 发明公开

EP1309848A2 METHOD AND APPARATUS FOR PREDICTIVE CELLULAR BIOINFORMATICS 审中-公开
标题翻译：方法和设备的预测ZELLBIOINFORMATIK
公开(公告)号：EP1309848A2
公开(公告)日：2003-05-14
申请号：EP01932627.1
申请日：2001-04-24
申请人： Cytokinetics, Inc.
发明人： OESTREICHER, Donald, R. , SABRY, James, H. , ADAMS, Cynthia, L. , VAISBERG, Eugeni, A. , CROMPTON, Anne, M.
IPC分类号： G01N15/14
CPC分类号： C12M41/46 , B01J2219/00315 , B01J2219/00689 , B01J2219/00695 , B01J2219/00707 , B01J2219/0072 , C40B60/14 , G01N15/1475 , G01N2015/1477
摘要： Techniques for using information technology in therapeutics or drug discovery. In an exemplary embodiment, techniques for determining information about the properties of substances based upon information about structure of living or non-living cells exposed to substances are provided. A method according to the present invention enables researchers and/or scientists to identify promising candidates in the search for new and better medicines or treatments using, for example, a multiple biological descriptors derived from a single cell component or marker. The method employs image analysis to extract a plurality of features (e.g., cell size, distance between cells, cell population, cell type) from an image acquisition device into the database.

6. 发明公开

EP1644805A2 METHODS AND APPARATUS FOR INVESTIGATING SIDE EFFECTS 审中-公开
标题翻译：方法和装置用于研究的不良反应
公开(公告)号：EP1644805A2
公开(公告)日：2006-04-12
申请号：EP04778160.4
申请日：2004-07-16
申请人： Cytokinetics, Inc.
发明人： KUTSYY, Vadim , VAISBERG, Eugeni, A. , COLEMAN, Daniel, A.
IPC分类号： G06F1/00
CPC分类号： G06K9/0014 , G01N33/5008 , G01N33/5014 , G01N33/502
摘要： Methods, apparatus, and computer programs for investigating and characterising side effects of a treatment having an intended or on-target effect on cells are described. The method can include identifying a group of on-target cellular features of the plurality of cells which are affected by the treatment and are related to the on-target effect. A group of off-target cellular features can also be identified which are different to the on-target cellular features and which are also affected by the treatment and which are related to the side effect. A measure of the side effect based on the off-target cellular features can be obtained. The treatment can then be characterised based on the measure of the side effect. A further method involves capturing an image of the population of treated cells and deriving cellular features from the image. An on-target effect signature, which is characteristic of the on-target effect is created from cellular features relating to cellular properties involved in the intended effect. A side effect signature, which is characteristic of a side effect to the on-target effect, is created using cellular features relating to cellular properties not involved in the intended effect. On target effect and/or side effect metrics are obtained from the signatures which can be used to characterise the treatment.

7. 发明公开

EP1188139A2 METHOD AND APPARATUS FOR PREDICTIVE CELLULAR BIOINFORMATICS 审中-公开
标题翻译：方法和装置用于蜂窝预测生物信息学
公开(公告)号：EP1188139A2
公开(公告)日：2002-03-20
申请号：EP00930696.0
申请日：2000-05-12
申请人： Cytokinetics, Inc.
发明人： SABRY, James, H. , ADAMS, Cynthia, L. , VAISBERG, Eugeni, A. , CROMPTON, Anne, M. , BLUM, Robert, I. , OESTREICHER, Donald, R. , SIGAL, Nolan, H.
IPC分类号： G06F19/00
摘要： Techniques for using information technology in therapeutics or drug discovery. In an exemplary embodiment, techniques for determining information about the properties of substances based upon information about structure of living or non-living cells exposed to substances are provided. A method according to the present invention enables researchers and/or scientists to identify promising candidates in the search for new and better medicines or treatments using, for example, a cellular informatics database. The present invention further teaches a system for acquiring knowledge from cellular information. The system has a database 1012 comprising a database management module ('DBMS'). The system also has a variety of modules, including a population module coupled to the DBMS for categorizing and storing a plurality of features (e.g., cell size, distance between cells, cell population, cell type) from an image acquisition device into the database. The system has a translation module coupled to the DBMS for defining a descriptor from a set of selected features from the plurality of features. In a specific embodiment, the descriptor is for a known or unknown compound, e.g., drug. A prediction module is coupled to the DBMS for selecting one of a plurality of descriptors from known and unknown compounds from the database based upon a selected descriptor from a selected compound. The selected compound may be one that is useful for treatment of human beings or the like.

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