专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060229818A1 Method for eliminating crosstalk between waveguide grating-based biosensors located in a microplate and the resulting microplate 失效
标题翻译：用于消除位于微板中的基于波导光栅的生物传感器与所得微板之间的串扰的方法
公开(公告)号：US20060229818A1
公开(公告)日：2006-10-12
申请号：US11101096
申请日：2005-04-07
申请人： Ye Fang , Ann Ferrie , Norman Fontaine , Anthony Frutos , Eric Mozdy , Chuan-Che Wang , Po Yuen
发明人： Ye Fang , Ann Ferrie , Norman Fontaine , Anthony Frutos , Eric Mozdy , Chuan-Che Wang , Po Yuen
IPC分类号： G06F19/00
CPC分类号： G01N21/7743 , G01N21/253
摘要： The present invention includes several methods for modifying the current processes of manufacturing optical sensing microplates that use continuous waveguide films to reduce/eliminate crosstalk between the biosensors that are incorporated within wells. The methods include (1) physically deteriorating/removing the waveguide film between individual biosensors; (2) chemically depositing highly absorbing materials within the waveguide film between individual biosensors; (3) patterning disordered (scattering) regions between the diffraction gratings that define individual biosensors; (4) using a specific mask and depositing individual patches of waveguide film, where each patch defines at least one biosensor. Each of these methods and several other methods described herein prevent the propagation of light between individual sensing regions, thereby eliminating optical crosstalk between the biosensors. The present invention also includes the resulting microplate.
摘要翻译：本发明包括几种用于修改制造光学感测微板的当前工艺的方法，其使用连续波导膜来减少/消除结合在孔内的生物传感器之间的串扰。所述方法包括（1）在各个生物传感器之间物理劣化/去除波导膜; （2）在各个生物传感器之间在波导膜内化学沉积高吸收材料; （3）在限定单个生物传感器的衍射光栅之间图形化无序（散射）区域; （4）使用特定掩模并沉积波导膜的各个贴片，其中每个贴片限定至少一个生物传感器。这些方法和本文所描述的其它方法中的每一种都防止光在各个感测区域之间的传播，从而消除了生物传感器之间的光学串扰。本发明还包括所得到的微孔板。

2. 发明申请

US20060141486A1 Porous substrates and arrays comprising the same 审中-公开
标题翻译：多孔基材和包含其的阵列
公开(公告)号：US20060141486A1
公开(公告)日：2006-06-29
申请号：US11027400
申请日：2004-12-29
申请人： Everett Coonan , Ye Fang , Ann Ferrie , Xiaodong Fu , Yulong Hong , Thomas Leslie , Xinghua Li , Beth Monahan , Eric Mozdy , Dirk Muller , Cameron Tanner , Patrick Tepesch , John Wight , Po Yuen
发明人： Everett Coonan , Ye Fang , Ann Ferrie , Xiaodong Fu , Yulong Hong , Thomas Leslie , Xinghua Li , Beth Monahan , Eric Mozdy , Dirk Muller , Cameron Tanner , Patrick Tepesch , John Wight , Po Yuen
IPC分类号： C12Q1/68 , G01N33/53 , C12M1/34 , B05D3/02
CPC分类号： B01L3/5085 , B01J2219/00315 , B01J2219/00317 , B01J2219/00423 , B01J2219/00639 , B01J2219/00644 , B01J2219/00722 , B01L2300/0819
摘要： The present invention relates to arrays comprising porous substrates for attachment of nucleic acids, polypeptides, membranes, or other biological or organic materials. In many embodiments, the arrays of the present invention have a flow-through configuration such that washing buffers or samples can access to the porous substrates from at least two sides of the arrays. The present invention also features arrays comprising UV-compatible porous substrates, arrays comprising three-dimensional membranes in sol-gels, and arrays comprising silica-based porous substrates prepared using a low-temperature fusion process.
摘要翻译：本发明涉及包含用于连接核酸，多肽，膜或其它生物或有机材料的多孔底物的阵列。在许多实施例中，本发明的阵列具有流通构型，使得洗涤缓冲液或样品可以从阵列的至少两侧进入多孔基底。本发明还具有包含UV兼容的多孔基材，溶胶凝胶中包含三维膜的阵列和包含使用低温熔融法制备的基于二氧化硅的多孔基材的阵列的阵列。

3. 发明申请

US20060223051A1 System and method for performing G protein coupled receptor (GPCR) cell assays using waveguide-grating sensors 审中-公开
标题翻译：使用波导光栅传感器进行G蛋白偶联受体（GPCR）细胞测定的系统和方法
公开(公告)号：US20060223051A1
公开(公告)日：2006-10-05
申请号：US11100262
申请日：2005-04-05
申请人： Ye Fang , Ann Ferrie , Norman Fontaine , Joydeep Lahiri , Po Yuen
发明人： Ye Fang , Ann Ferrie , Norman Fontaine , Joydeep Lahiri , Po Yuen
IPC分类号： G01N33/53 , C12Q1/00
CPC分类号： G01N33/5041 , G01N21/4788 , G01N21/553 , G01N2333/726 , G01N2500/10
摘要： The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.
摘要翻译：本发明包括使用光学LID生物传感器实时监测活细胞内激动剂诱导的GPCR信号事件的系统和方法。特别地，本发明包括使用光学LID生物传感器基于由于激动剂诱导的GPCR活化导致的质量再分布来筛选活细胞内的靶GPCR的化合物的系统和方法。在扩展实施例中，本发明公开了用于自参考光学LID生物传感器以消除对环境温度，压力波动和其他环境变化的不期望的灵敏度的不同方式。在又一个扩展的实施方案中，本发明公开了在单一培养基溶液中的多种类型的细胞中单种类型细胞或多种GPCR筛选多种GPCR的不同方式。在又一个延伸的实施方案中，本发明公开了确定化合物对活细胞内特异性GPCR的生理或药理作用的不同方式。

4. 发明申请

US20070141641A1 Compositions & methods for reformulating biological membranes for arrays 审中-公开
标题翻译：用于阵列生物膜重组的组合物和方法
公开(公告)号：US20070141641A1
公开(公告)日：2007-06-21
申请号：US11699650
申请日：2007-01-30
申请人： Ye Fang , Ann Ferrie
发明人： Ye Fang , Ann Ferrie
IPC分类号： G01N33/567 , G01N33/53
CPC分类号： C40B50/08 , C40B40/00 , G01N2333/726
摘要： A standardized, buffered solution or “ink” composition for re-formulating or suspending biological membranes used in the fabrication of membrane arrays. The composition can enhance assay performance and prolong the shelf life of biological membrane arrays. The ink composition comprises a combination of at least two of the following six classes of reagents: 1) a pH buffer reagent; 2) a monovalent or divalent, inorganic salt; 3) a membrane stabilizer; 4) a solution viscosity control reagent; 5) a water-soluble protein; or, 6) a protease inhibitor. A method for fabricating a membrane array using the present ink composition is also described.
摘要翻译：用于重新配制或悬浮用于制造膜阵列的生物膜的标准化缓冲溶液或“油墨”组合物。该组合物可以增强测定性能并延长生物膜阵列的保质期。油墨组合物包含以下六类试剂中的至少两种的组合：1）pH缓冲剂; 2）一价或二价无机盐; 3）膜稳定剂; 4）溶液粘度控制试剂; 5）水溶性蛋白质; 或6）蛋白酶抑制剂。还描述了使用本发明的油墨组合物制造膜阵列的方法。

5. 发明申请

US20050009204A1 Multiplexed binding assays for receptor arrays 审中-公开
标题翻译：受体阵列的多重结合测定
公开(公告)号：US20050009204A1
公开(公告)日：2005-01-13
申请号：US10639718
申请日：2003-08-12
申请人： Ye Fang , Yulong Hong , Joydeep Lahiri , Fang Lai , Jinlin Peng , Brian Webb , Ann Ferrie
发明人： Ye Fang , Yulong Hong , Joydeep Lahiri , Fang Lai , Jinlin Peng , Brian Webb , Ann Ferrie
IPC分类号： C40B30/04 , G01N33/68 , G01N33/74 , G01N33/543
CPC分类号： C40B30/04 , G01N33/74 , G01N2333/726
摘要： A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.
摘要翻译：提供了一种专题微阵列和在存在或不存在目标化合物的情况下使用标记配体的混合溶液进行多重结合测定的方法。这些方法使研究人员可以使用微阵列格式来筛选多个靶点的化合物。

6. 发明申请

US20050048648A1 Compositions & methods for reformulating biological membranes for arrays 审中-公开
标题翻译：用于阵列生物膜重组的组合物和方法
公开(公告)号：US20050048648A1
公开(公告)日：2005-03-03
申请号：US10651554
申请日：2003-08-29
申请人： Ye Fang , Ann Ferrie
发明人： Ye Fang , Ann Ferrie
IPC分类号： C12N5/02
CPC分类号： C40B50/08 , C40B40/00 , G01N2333/726
摘要： A standardized, buffered solution or “ink” composition for re-formulating or suspending biological membranes used in the fabrication of membrane arrays. The composition can enhance assay performance and prolong the shelf life of biological membrane arrays. The ink composition comprises a combination of at least two of the following six classes of reagents: 1) a pH buffer reagent; 2) a monovalent or divalent, inorganic salt; 3) a membrane stabilizer; 4) a solution viscosity control reagent; 5) a water-soluble protein; or, 6) a protease inhibitor. A method for fabricating a membrane array using the present ink composition is also described.
摘要翻译：用于重新配制或悬浮用于制造膜阵列的生物膜的标准化缓冲溶液或“油墨”组合物。该组合物可以增强测定性能并延长生物膜阵列的保质期。油墨组合物包含以下六类试剂中的至少两种的组合：1）pH缓冲剂; 2）一价或二价无机盐; 3）膜稳定剂; 4）溶液粘度控制试剂; 5）水溶性蛋白质; 或6）蛋白酶抑制剂。还描述了使用本发明的油墨组合物制造膜阵列的方法。

7. 发明申请

US20060148006A1 Assay solution compositions and methods for GPCR arrays 失效
标题翻译：测定GPCR阵列的溶液组成和方法
公开(公告)号：US20060148006A1
公开(公告)日：2006-07-06
申请号：US11312776
申请日：2005-12-21
申请人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
发明人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
IPC分类号： G01N33/567 , G01N33/53
CPC分类号： G01N33/52 , G01N2333/726
摘要： Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.
摘要翻译：描述了用于执行1）GPCR阵列上的结合或2）功能测定的缓冲测定溶液及其使用方法。缓冲测定溶液具有下列组成，其具有：pH在约6.5至约7.9范围内的缓冲试剂; 浓度为约1mM至约500mM的单价或二价物质的无机盐; 和任选的以下组合：c）浓度为约0.01wt。％至约2wt。％的组合物，或d）浓度为约0.001mM至约100mM的蛋白酶抑制剂。在功能测定用途的实施方案中，组合物被修饰为还包括GTP类似物，鸟苷5'-二磷酸（GDP）盐和/或抗氧化试剂。

8. 发明申请

US20050227241A1 Porous substrate plates and the use thereof 失效
标题翻译：多孔基板及其用途
公开(公告)号：US20050227241A1
公开(公告)日：2005-10-13
申请号：US10822385
申请日：2004-04-12
申请人： Ye Fang , Ann Ferrie , Yulong Hong , Brian Webb
发明人： Ye Fang , Ann Ferrie , Yulong Hong , Brian Webb
IPC分类号： B01L3/00 , C12M1/34 , C12Q1/68 , G01N33/53
CPC分类号： B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/0829 , Y10S435/81 , Y10S436/809 , Y10T29/49885
摘要： A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three- dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.
摘要翻译：公开了适用于生物或化学测定的底物板或装置。该装置可以采用具有三维多孔层的多孔板的形式，作为每个孔内的支撑表面的一部分，用于固定探针物质。多孔层的特征在于具有由连续的固体材料的基体限定的多个相互连接的空隙。还描述了一种方法及其变体。

9. 发明申请

US20050069953A1 Assay solution compositions and methods for GPCR arrays 审中-公开
标题翻译：测定GPCR阵列的溶液组成和方法
公开(公告)号：US20050069953A1
公开(公告)日：2005-03-31
申请号：US10676351
申请日：2003-09-30
申请人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
发明人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
IPC分类号： G01N33/52 , G01N33/53
CPC分类号： G01N33/52 , G01N2333/726
摘要： Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.
摘要翻译：描述了用于执行1）GPCR阵列上的结合或2）功能测定的缓冲测定溶液及其使用方法。缓冲测定溶液具有下列组成，其具有：pH在约6.5至约7.9范围内的缓冲试剂; 浓度为约1mM至约500mM的单价或二价物质的无机盐; 和任选的以下组合：c）浓度为约0.01wt。％至约2wt。％的组合物，或d）浓度为约0.001mM至约100mM的蛋白酶抑制剂。在功能测定用途的实施方案中，组合物被修饰为还包括GTP类似物，鸟苷5'-二磷酸（GDP）盐和/或抗氧化试剂。

10. 发明申请

US20050089911A1 87 human secreted proteins 失效
标题翻译： 87人分泌蛋白
公开(公告)号：US20050089911A1
公开(公告)日：2005-04-28
申请号：US10960251
申请日：2004-10-08
申请人： Paul Young , John Greene , Ann Ferrie , Steven Ruben , Craig Rosen , Roxanne Duan , Jing-Shan Hu , Kimberly Florence , Henrik Olsen , Reinhard Ebner , Laurie Brewer , Paul Moore , Yanggu Shi , David LaFleur , Jian Ni
发明人： Paul Young , John Greene , Ann Ferrie , Steven Ruben , Craig Rosen , Roxanne Duan , Jing-Shan Hu , Kimberly Florence , Henrik Olsen , Reinhard Ebner , Laurie Brewer , Paul Moore , Yanggu Shi , David LaFleur , Jian Ni
IPC分类号： C12N15/09 , A61K31/711 , A61K38/00 , A61K48/00 , A61P3/02 , A61P3/04 , A61P3/10 , A61P7/02 , A61P7/06 , A61P9/10 , A61P9/12 , A61P11/06 , A61P13/12 , A61P15/08 , A61P15/16 , A61P17/00 , A61P17/02 , A61P17/06 , A61P19/02 , A61P19/10 , A61P21/04 , A61P25/00 , A61P25/16 , A61P25/18 , A61P25/20 , A61P25/28 , A61P25/30 , A61P27/02 , A61P29/00 , A61P35/00 , A61P35/02 , A61P37/04 , A61P37/06 , A61P37/08 , C07K14/47 , C07K16/18 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/68 , C07H21/04 , C12N9/00 , C12P21/04
CPC分类号： C12Q1/6883 , A61K38/00 , C07K14/47 , G01N33/6893
摘要： The present invention relates to 87 novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.
摘要翻译：本发明涉及87种新的人分泌蛋白和含有编码这种蛋白质的基因的编码区的分离的核酸。还提供了用于产生人分泌蛋白质的载体，宿主细胞，抗体和重组方法。本发明还涉及用于诊断和治疗与这些新型人分泌蛋白相关的病症的诊断和治疗方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式