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1. 发明申请

US20070020689A1 Label-free high throughput biomolecular screening system and method 审中-公开
标题翻译：无标记的高通量生物分子筛选系统和方法
公开(公告)号：US20070020689A1
公开(公告)日：2007-01-25
申请号：US11489173
申请日：2006-07-19
申请人： Stephen Caracci , Volker Eckelt , Anthony Frutos , Mark Krol , Thomas Moore , David Pastel , Gordon Shedd
发明人： Stephen Caracci , Volker Eckelt , Anthony Frutos , Mark Krol , Thomas Moore , David Pastel , Gordon Shedd
IPC分类号： C40B30/06 , G01N33/53 , G06F19/00 , C12M1/34
CPC分类号： G01N35/028 , G01N2035/00158 , G01N2035/00356 , G01N2035/0097
摘要： A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occurr in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays.
摘要翻译：本文描述了一种筛选系统和方法，其提供用于实现无标记高通量筛选（HTS）以帮助发现新药物的独特且实用的解决方案。在一个实施方案中，筛选系统使得能够进行直接结合测定，其中化学化合物（药物候选物）与生物分子（治疗靶标）的生物分子相互作用可以使用与当前实践相兼容的测定体积和浓度来检测 HTS在制药行业。筛选系统还能够检测在微板的孔中发生的生物化学相互作用，其结合生物传感器和表面化学以将治疗靶固定在生物传感器的表面。筛选系统还包括流体处理和板处理装置，以帮助执行自动HTS测定。

2. 发明申请

US20060229818A1 Method for eliminating crosstalk between waveguide grating-based biosensors located in a microplate and the resulting microplate 失效
标题翻译：用于消除位于微板中的基于波导光栅的生物传感器与所得微板之间的串扰的方法
公开(公告)号：US20060229818A1
公开(公告)日：2006-10-12
申请号：US11101096
申请日：2005-04-07
申请人： Ye Fang , Ann Ferrie , Norman Fontaine , Anthony Frutos , Eric Mozdy , Chuan-Che Wang , Po Yuen
发明人： Ye Fang , Ann Ferrie , Norman Fontaine , Anthony Frutos , Eric Mozdy , Chuan-Che Wang , Po Yuen
IPC分类号： G06F19/00
CPC分类号： G01N21/7743 , G01N21/253
摘要： The present invention includes several methods for modifying the current processes of manufacturing optical sensing microplates that use continuous waveguide films to reduce/eliminate crosstalk between the biosensors that are incorporated within wells. The methods include (1) physically deteriorating/removing the waveguide film between individual biosensors; (2) chemically depositing highly absorbing materials within the waveguide film between individual biosensors; (3) patterning disordered (scattering) regions between the diffraction gratings that define individual biosensors; (4) using a specific mask and depositing individual patches of waveguide film, where each patch defines at least one biosensor. Each of these methods and several other methods described herein prevent the propagation of light between individual sensing regions, thereby eliminating optical crosstalk between the biosensors. The present invention also includes the resulting microplate.
摘要翻译：本发明包括几种用于修改制造光学感测微板的当前工艺的方法，其使用连续波导膜来减少/消除结合在孔内的生物传感器之间的串扰。所述方法包括（1）在各个生物传感器之间物理劣化/去除波导膜; （2）在各个生物传感器之间在波导膜内化学沉积高吸收材料; （3）在限定单个生物传感器的衍射光栅之间图形化无序（散射）区域; （4）使用特定掩模并沉积波导膜的各个贴片，其中每个贴片限定至少一个生物传感器。这些方法和本文所描述的其它方法中的每一种都防止光在各个感测区域之间的传播，从而消除了生物传感器之间的光学串扰。本发明还包括所得到的微孔板。

3. 发明申请

US20060223184A1 Supports useful in incorporating biomolecules into cells and methods of using thereof 审中-公开
标题翻译：用于将生物分子并入细胞中的支持物及其使用方法
公开(公告)号：US20060223184A1
公开(公告)日：2006-10-05
申请号：US11099904
申请日：2005-04-05
申请人： Anthony Frutos , Joydeep Lahiri , Santona Pal , Elizabeth Tran , Brian Webb
发明人： Anthony Frutos , Joydeep Lahiri , Santona Pal , Elizabeth Tran , Brian Webb
IPC分类号： C12N15/88 , C12N15/86
CPC分类号： C12N15/87
摘要： Described herein are supports useful in incorporating biomolecules into cells and methods of making and using thereof.
摘要翻译：本文描述的是用于将生物分子并入细胞中的支持物及其制备和使用方法。

4. 发明申请

US20050260685A1 Arrays of biological membranes and methods and use thereof 审中-公开
标题翻译：生物膜阵列及其用途
公开(公告)号：US20050260685A1
公开(公告)日：2005-11-24
申请号：US11173974
申请日：2005-07-01
申请人： Ye Fang , Anthony Frutos , Steven Jonas , Peter Kalal , Joydeep Lahiri
发明人： Ye Fang , Anthony Frutos , Steven Jonas , Peter Kalal , Joydeep Lahiri
IPC分类号： G01N27/62 , B01J19/00 , C07K1/04 , C12M1/34 , C40B40/10 , C40B60/14 , G01N21/41 , G01N21/64 , G01N21/76 , G01N27/02 , G01N33/53 , G01N33/543 , G01N33/545 , G01N33/547 , G01N33/552 , G01N33/553 , G01N33/566 , G01N33/68 , G01N33/74 , G01N37/00
CPC分类号： B01J19/0046 , B01J2219/00385 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00691 , B01J2219/00707 , B01J2219/00725 , B01J2219/0074 , B82Y15/00 , B82Y30/00 , C07K1/047 , C40B40/10 , C40B60/14 , G01N33/5438 , G01N33/566 , G01N33/6845 , G01N33/6872 , G01N33/74 , G01N2333/726 , G01N2500/02
摘要： The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.
摘要翻译：本发明克服了与现有技术阵列相关的问题和缺点，提供了一种阵列，其包括与基底表面相关联的多个生物膜微孔，该生物膜微孔可以在水溶液环境中生产，使用和储存，而不是在环境中在环境或受控的湿度下暴露于空气。优选地，生物膜微点包含膜结合蛋白。最优选地，膜结合蛋白是G蛋白偶联受体，离子通道，受体丝氨酸/苏氨酸激酶或受体酪氨酸激酶。

5. 发明申请

US20070154348A1 Supports for assaying analytes and methods of making and using thereof 有权
标题翻译：支持测定分析物及其制备和使用方法
公开(公告)号：US20070154348A1
公开(公告)日：2007-07-05
申请号：US11448486
申请日：2006-06-07
申请人： Anthony Frutos , David Henry
发明人： Anthony Frutos , David Henry
IPC分类号： G01N21/00
CPC分类号： G01N33/54353 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00736 , B01J2219/0074 , C08F8/00 , C08F8/32 , Y10T436/143333 , C08F210/02 , C08F222/06
摘要： Described herein are supports for assaying an analyte and methods of making and using thereof.
摘要翻译：本文描述了用于测定分析物的支持物及其制备和使用方法。

6. 发明申请

US20060257919A1 Polymer-coated substrates for binding biological molecules 审中-公开
标题翻译：用于结合生物分子的聚合物涂覆的底物
公开(公告)号：US20060257919A1
公开(公告)日：2006-11-16
申请号：US11488228
申请日：2006-07-18
申请人： Anthony Frutos , Joydeep Lahiri , Thomas Leslie , Jinlin Peng
发明人： Anthony Frutos , Joydeep Lahiri , Thomas Leslie , Jinlin Peng
IPC分类号： C40B40/08 , C40B50/14
CPC分类号： B82Y30/00 , G01N33/54393 , G01N33/548
摘要： A substrate, which that is capable of attaching biomolecules, and a method for preparing the substrate are provided. The substrate has a reactive surface that can covalently attach a polymer coating containing functional groups, which can reduce nonspecific binding of biomolecules to the surface for a biological array. Optionally, at least a portion of the substrate may be coated with an intermediate tie layer, which enhances the covalent bonding between the polymer coating with the underlying substrate. The present invention also pertains to a method that uses electrostatic blocking agents to reduce non-specific binding of proteins to a substrate, especially anhydride-modified surfaces.
摘要翻译：提供了能够附着生物分子的基材和制备基材的方法。基底具有可以共价连接含有官能团的聚合物涂层的反应性表面，其可以降低生物分子与生物分子的表面的非特异性结合。任选地，衬底的至少一部分可以涂覆有中间粘结层，其增强聚合物涂层与下面的衬底之间的共价键。本发明还涉及使用静电阻断剂以减少蛋白质与底物，特别是酸酐改性表面的非特异性结合的方法。

7. 发明申请

US20060205058A1 Spatially scanned optical reader system and method for using same 审中-公开
公开(公告)号：US20060205058A1
公开(公告)日：2006-09-14
申请号：US11437477
申请日：2006-05-18
申请人： Anthony Frutos , Jacques Gollier , Jinlin Peng , Garrett Piech , Michael Webb
发明人： Anthony Frutos , Jacques Gollier , Jinlin Peng , Garrett Piech , Michael Webb
IPC分类号： C12M1/34
CPC分类号： G01N21/253 , G01N21/554 , G01N21/7743
摘要： An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP). The light source outputs an optical beam which is scanned across a moving biosensor and while this is happening the detector collects the optical beam which is reflected from the biosensor. The computer processes the collected optical beam and records the resulting raw spectral or angle data which is a function of a position (and possibly time) on the biosensor. The processor can then analyze the raw data to create a spatial map of resonant wavelength (peak position) or resonant angle which indicates whether or not a biomolecular binding event occurred on the biosensor. Several other uses of the raw data are also described herein.

8. 发明申请

US20060141611A1 Spatially scanned optical reader system and method for using same 失效
公开(公告)号：US20060141611A1
公开(公告)日：2006-06-29
申请号：US11027547
申请日：2004-12-29
申请人： Anthony Frutos , Jacques Gollier , Jinlin Peng , Garrett Piech , Michael Webb
发明人： Anthony Frutos , Jacques Gollier , Jinlin Peng , Garrett Piech , Michael Webb
IPC分类号： C12M1/34
CPC分类号： G01N21/253 , G01N21/554 , G01N21/7743
摘要： An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP). The light source outputs an optical beam which is scanned across a moving biosensor and while this is happening the detector collects the optical beam which is reflected from the biosensor. The computer processes the collected optical beam and records the resulting raw spectral or angle data which is a function of a position (and possibly time) on the biosensor. The processor can then analyze the raw data to create a spatial map of resonant wavelength (peak position) or resonant angle which indicates whether or not a biomolecular binding event occurred on the biosensor. Several other uses of the raw data are also described herein.

9. 发明申请

US20060141527A1 Method for creating a reference region and a sample region on a biosensor and the resulting biosensor 审中-公开
标题翻译：用于在生物传感器上产生参考区域和样本区域的方法以及由此产生的生物传感器
公开(公告)号：US20060141527A1
公开(公告)日：2006-06-29
申请号：US11027509
申请日：2004-12-29
申请人： Stephen Caracci , Anthony Frutos , Jinlin Peng , Garrett Piech , Michael Webb
发明人： Stephen Caracci , Anthony Frutos , Jinlin Peng , Garrett Piech , Michael Webb
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： B01L3/5085 , B01J19/0046 , B01J2219/00315 , B01J2219/00367 , B01J2219/00378 , B01J2219/00382 , B01J2219/00385 , B01J2219/00387 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00662 , B01J2219/00677 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , B01L2200/12 , B01L2200/148 , B01L2300/0636 , B01L2300/0829 , G01N21/553 , G01N21/7743 , G01N2035/00158
摘要： A method is described herein that can use any one of a number of deposition techniques to create a reference region and a sample region on a single biosensor which in the preferred embodiment is located within a single well of a microplate. The deposition techniques that can be used to help create the reference region and the sample region on a surface of the biosensor include: (1) the printing/stamping of a deactivating agent on a reactive surface of the biosensor; (2) the printing/stamping of a target molecule (target protein) on a reactive surface of the biosensor; or (3) the printing/stamping of a reactive agent on an otherwise unreactive surface of the biosensor.
摘要翻译：本文描述的方法可以使用多个沉积技术中的任何一种来在单个生物传感器上产生参考区域和样品区域，其在优选实施例中位于微板的单个孔内。可用于帮助创建生物传感器表面上的参考区域和样品区域的沉积技术包括：（1）在生物传感器的反应性表面上印刷/冲压钝化剂; （2）生物传感器的反应性表面上的靶分子（靶蛋白）的印刷/印模; 或（3）在生物传感器的其它非反应性表面上印刷/冲压反应剂。

10. 发明申请

US20060110594A1 Polymer-coated substrates for binding biomolecules and methods of making and using thereof 审中-公开
标题翻译：用于结合生物分子的聚合物涂覆的底物及其制备和使用方法
公开(公告)号：US20060110594A1
公开(公告)日：2006-05-25
申请号：US10996952
申请日：2004-11-24
申请人： Anthony Frutos , Joydeep Lahiri , Jinlin Peng , Odessa Petzold
发明人： Anthony Frutos , Joydeep Lahiri , Jinlin Peng , Odessa Petzold
IPC分类号： B32B27/30 , B32B27/00 , G01N31/22
CPC分类号： G01N33/54353 , B82Y30/00 , C03C17/3405 , G01N21/553 , G01N21/648 , G01N33/545 , Y10T428/26 , Y10T428/269 , Y10T428/31504 , Y10T428/31536 , Y10T428/31663 , Y10T428/31935
摘要： Described herein are polymer-coated substrates for binding biomolecules and methods of making and using thereof.
摘要翻译：本文描述了用于结合生物分子的聚合物涂覆的底物及其制备和使用方法。

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