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1. 发明申请

US20060148006A1 Assay solution compositions and methods for GPCR arrays 失效
标题翻译：测定GPCR阵列的溶液组成和方法
公开(公告)号：US20060148006A1
公开(公告)日：2006-07-06
申请号：US11312776
申请日：2005-12-21
申请人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
发明人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
IPC分类号： G01N33/567 , G01N33/53
CPC分类号： G01N33/52 , G01N2333/726
摘要： Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.
摘要翻译：描述了用于执行1）GPCR阵列上的结合或2）功能测定的缓冲测定溶液及其使用方法。缓冲测定溶液具有下列组成，其具有：pH在约6.5至约7.9范围内的缓冲试剂; 浓度为约1mM至约500mM的单价或二价物质的无机盐; 和任选的以下组合：c）浓度为约0.01wt。％至约2wt。％的组合物，或d）浓度为约0.001mM至约100mM的蛋白酶抑制剂。在功能测定用途的实施方案中，组合物被修饰为还包括GTP类似物，鸟苷5'-二磷酸（GDP）盐和/或抗氧化试剂。

2. 发明申请

US20050069953A1 Assay solution compositions and methods for GPCR arrays 审中-公开
标题翻译：测定GPCR阵列的溶液组成和方法
公开(公告)号：US20050069953A1
公开(公告)日：2005-03-31
申请号：US10676351
申请日：2003-09-30
申请人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
发明人： Ye Fang , Ann Ferrie , Yulong Hong , Sadashiva Pai , Jinlin Peng , Brian Webb
IPC分类号： G01N33/52 , G01N33/53
CPC分类号： G01N33/52 , G01N2333/726
摘要： Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.
摘要翻译：描述了用于执行1）GPCR阵列上的结合或2）功能测定的缓冲测定溶液及其使用方法。缓冲测定溶液具有下列组成，其具有：pH在约6.5至约7.9范围内的缓冲试剂; 浓度为约1mM至约500mM的单价或二价物质的无机盐; 和任选的以下组合：c）浓度为约0.01wt。％至约2wt。％的组合物，或d）浓度为约0.001mM至约100mM的蛋白酶抑制剂。在功能测定用途的实施方案中，组合物被修饰为还包括GTP类似物，鸟苷5'-二磷酸（GDP）盐和/或抗氧化试剂。

3. 发明申请

US20050009204A1 Multiplexed binding assays for receptor arrays 审中-公开
标题翻译：受体阵列的多重结合测定
公开(公告)号：US20050009204A1
公开(公告)日：2005-01-13
申请号：US10639718
申请日：2003-08-12
申请人： Ye Fang , Yulong Hong , Joydeep Lahiri , Fang Lai , Jinlin Peng , Brian Webb , Ann Ferrie
发明人： Ye Fang , Yulong Hong , Joydeep Lahiri , Fang Lai , Jinlin Peng , Brian Webb , Ann Ferrie
IPC分类号： C40B30/04 , G01N33/68 , G01N33/74 , G01N33/543
CPC分类号： C40B30/04 , G01N33/74 , G01N2333/726
摘要： A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.
摘要翻译：提供了一种专题微阵列和在存在或不存在目标化合物的情况下使用标记配体的混合溶液进行多重结合测定的方法。这些方法使研究人员可以使用微阵列格式来筛选多个靶点的化合物。

4. 发明申请

US20050227241A1 Porous substrate plates and the use thereof 失效
标题翻译：多孔基板及其用途
公开(公告)号：US20050227241A1
公开(公告)日：2005-10-13
申请号：US10822385
申请日：2004-04-12
申请人： Ye Fang , Ann Ferrie , Yulong Hong , Brian Webb
发明人： Ye Fang , Ann Ferrie , Yulong Hong , Brian Webb
IPC分类号： B01L3/00 , C12M1/34 , C12Q1/68 , G01N33/53
CPC分类号： B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/0829 , Y10S435/81 , Y10S436/809 , Y10T29/49885
摘要： A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three- dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.
摘要翻译：公开了适用于生物或化学测定的底物板或装置。该装置可以采用具有三维多孔层的多孔板的形式，作为每个孔内的支撑表面的一部分，用于固定探针物质。多孔层的特征在于具有由连续的固体材料的基体限定的多个相互连接的空隙。还描述了一种方法及其变体。

5. 发明申请

US20060147993A1 Membrane arrays and methods of manufacture 失效
公开(公告)号：US20060147993A1
公开(公告)日：2006-07-06
申请号：US11026371
申请日：2004-12-30
申请人： Alain Carre , Alexander Efrenov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John Mauro , Srikanth Raghavan , Brian Webb
发明人： Alain Carre , Alexander Efrenov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John Mauro , Srikanth Raghavan , Brian Webb
IPC分类号： G01N33/53 , G06F19/00 , C12M1/34 , B05D3/02
CPC分类号： G01N33/554 , G01N33/74 , G01N2333/726
摘要： The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.

6. 发明申请

US20080103059A1 Microcolumn-platform based array for high-throughput analysis 审中-公开
公开(公告)号：US20080103059A1
公开(公告)日：2008-05-01
申请号：US12001850
申请日：2007-12-13
申请人： Brian Webb , Jinlin Peng , Michael Brady , Mircea Despa , Keith Horn , Joydeep Lahiri , David Root , James Stamatoff
发明人： Brian Webb , Jinlin Peng , Michael Brady , Mircea Despa , Keith Horn , Joydeep Lahiri , David Root , James Stamatoff
IPC分类号： C40B30/04
CPC分类号： B01L3/50853 , B01J2219/00317 , B01J2219/00504 , B01J2219/00511 , B01J2219/00585 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00662 , B01J2219/00707 , B01L3/5025 , B01L2200/025 , B01L2200/026 , B01L2300/046 , B01L2300/0636 , B01L2300/0829 , C12Q1/6837 , C40B60/14 , G01N33/54366 , G01N33/54373 , G01N33/54386 , G01N35/028
摘要： A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.

7. 发明申请

US20060105371A1 Method and device for protein delivery into cells 失效
公开(公告)号：US20060105371A1
公开(公告)日：2006-05-18
申请号：US11261198
申请日：2005-10-27
申请人： Ye Fang , Fang Lai , Laurent Picard , Brian Webb
发明人： Ye Fang , Fang Lai , Laurent Picard , Brian Webb
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

8. 发明申请

US20140066595A1 Modulators of Protein Production in a Human Cell Line and Cell-free Extracts Produced Therefrom 审中-公开
标题翻译：人类细胞系中的蛋白质生产调节剂及其生产的无细胞提取物
公开(公告)号：US20140066595A1
公开(公告)日：2014-03-06
申请号：US14010246
申请日：2013-08-26
申请人： Emily Anderson , Peter Bell , Penny Jensen , Jon Karpilow , Anja Smith , Krishna Vattem , Brian Webb , Devin Leake , Alex Medford , Elena Maksimova , Craig Smith
发明人： Emily Anderson , Peter Bell , Penny Jensen , Jon Karpilow , Anja Smith , Krishna Vattem , Brian Webb , Devin Leake , Alex Medford , Elena Maksimova , Craig Smith
IPC分类号： C12P21/00 , C07K14/00
CPC分类号： C12P21/00 , C07K14/00
摘要： Cell-free extracts, methods for producing these extracts, methods for using these extracts, compositions that facilitate production of these extracts and kits that contain these extracts are provided. By increasing or decreasing certain gene products through, for example, the use of siRNA or mimics, one can develop mammalian cell-free extracts that have desired levels of efficiency.
摘要翻译：提供了无细胞提取物，用于生产这些提取物的方法，使用这些提取物的方法，促进这些提取物生产的组合物和含有这些提取物的试剂盒。通过例如使用siRNA或模拟物来增加或减少某些基因产物，可以开发具有期望的效率水平的哺乳动物无细胞提取物。

9. 发明授权

US08974147B1 Slot form for pipeline buoyancy control 有权
标题翻译：管道浮力控制的槽型
公开(公告)号：US08974147B1
公开(公告)日：2015-03-10
申请号：US14508446
申请日：2014-10-07
申请人： Brian Webb
发明人： Brian Webb
IPC分类号： F16L1/06 , F16L3/04
CPC分类号： F16L1/24 , F16L1/06
摘要： A device for controlling buoyancy of a buried pipeline, the device comprising a form placed atop the pipeline and a flexible strap extending around the form adjacent its top. The form may comprise a first trough located adjacent the first side of the pipeline, a second trough located adjacent the opposing second side of the pipeline, and a middle section with an inverted-U-shaped cross section such that the middle portion fits over the top of the pipeline and joins the first trough and the second trough. The troughs are filled with backfill when the pipeline is buried. Thus, when fully installed, the device may comprise the slot form, the strap, and the backfill. As the pipeline tends to rise, the straps deform, bringing the sides of the troughs inward and compressing the backfill therein. This prevents further upward movement of the pipeline.
摘要翻译：一种用于控制埋设管道浮力的装置，该装置包括放置在管道顶部的形式和围绕邻近其顶部的形式延伸的柔性带。该形式可以包括邻近管道的第一侧定位的第一槽，邻近管道相对的第二侧的第二槽和具有倒U形横截面的中间部分，使得中间部分配合在管道顶部并连接第一槽和第二槽。当管道埋设时，槽填充回填。因此，当完全安装时，设备可以包括槽形式，带子和回填。随着管道趋于上升，带子变形，将槽的两侧向内压缩并压缩其中的回填。这防止了管道的进一步向上移动。

10. 发明申请

US20060223184A1 Supports useful in incorporating biomolecules into cells and methods of using thereof 审中-公开
标题翻译：用于将生物分子并入细胞中的支持物及其使用方法
公开(公告)号：US20060223184A1
公开(公告)日：2006-10-05
申请号：US11099904
申请日：2005-04-05
申请人： Anthony Frutos , Joydeep Lahiri , Santona Pal , Elizabeth Tran , Brian Webb
发明人： Anthony Frutos , Joydeep Lahiri , Santona Pal , Elizabeth Tran , Brian Webb
IPC分类号： C12N15/88 , C12N15/86
CPC分类号： C12N15/87
摘要： Described herein are supports useful in incorporating biomolecules into cells and methods of making and using thereof.
摘要翻译：本文描述的是用于将生物分子并入细胞中的支持物及其制备和使用方法。

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