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1. 发明授权

US08741660B2 Sampling device 失效
标题翻译：取样装置
公开(公告)号：US08741660B2
公开(公告)日：2014-06-03
申请号：US12732494
申请日：2010-03-26
申请人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
发明人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
IPC分类号： G01N1/18
CPC分类号： B01L3/021 , B01L3/0293 , B01L7/52 , B01L2200/0642 , B01L2200/0673 , Y10T436/25 , Y10T436/25625
摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.
摘要翻译：本发明一般涉及用于获取和/或分配样品而不将气体引入诸如液体桥系统的微流体系统中的装置，系统和方法。示例性实施例提供了一种包括外护套的取样装置; 鞘内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可扩展超出护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

2. 发明申请

US20100304443A1 SAMPLING DEVICE 失效
标题翻译：采样设备
公开(公告)号：US20100304443A1
公开(公告)日：2010-12-02
申请号：US12732494
申请日：2010-03-26
申请人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
发明人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
IPC分类号： C12P19/34 , C12M1/26
CPC分类号： B01L3/021 , B01L3/0293 , B01L7/52 , B01L2200/0642 , B01L2200/0673 , Y10T436/25 , Y10T436/25625
摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.
摘要翻译：本发明一般涉及用于获取和/或分配样品而不将气体引入诸如液体桥系统的微流体系统中的装置，系统和方法。示例性实施例提供了一种包括外护套的取样装置; 鞘内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可扩展超出护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

3. 发明申请

US20140120604A1 Biological Detection System and Method of Use 审中-公开
标题翻译：生物检测系统及其使用方法
公开(公告)号：US20140120604A1
公开(公告)日：2014-05-01
申请号：US14110635
申请日：2012-04-06
申请人： Mauro Aguanno , Brian Barrett , Brian Chawke , Kieran Curran , Damian Curtin , Tara Dalton , Mark Davies , Xiaona Hou , David Kinahan , Damien King , Mark Korenke , Conor McCarthy , David McGuire , Michael Sayers , Noel Sirr , Ryan J. Talbot
发明人： Mauro Aguanno , Brian Barrett , Brian Chawke , Kieran Curran , Damian Curtin , Tara Dalton , Mark Davies , Xiaona Hou , David Kinahan , Damien King , Mark Korenke , Conor McCarthy , David McGuire , Michael Sayers , Noel Sirr , Ryan J. Talbot
IPC分类号： G01N21/64 , B01F13/00
CPC分类号： C12Q1/686 , B01F13/0005 , B01F13/0071 , B01F15/00214 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/143 , B01L2300/06 , B01L2300/0654 , B01L2300/0867 , G01N21/6428 , G01N35/00623 , G01N2021/6439 , G01N2021/6441 , G01N2035/00465 , G01N2201/062
摘要： Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred.
摘要翻译：本文提供了一种生物检测系统和使用方法，其中生物检测系统包括用于组合至少两个液滴的至少一个混合器或液体桥，以及用于检测是否适当混合或组合两个组分液滴的误差校正系统已经发生

4. 发明授权

US09631230B2 Methods and devices for digital PCR 有权
公开(公告)号：US09631230B2
公开(公告)日：2017-04-25
申请号：US12539343
申请日：2009-08-11
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68 , B01L3/00 , B01L7/00
CPC分类号： C12Q1/6851 , B01L3/502784 , B01L7/525 , B01L2300/0838 , B01L2300/0867 , B01L2400/0487 , C12Q2561/101 , C12Q2563/159 , C12Q2565/629
摘要： The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.

5. 发明授权

US08298833B2 Liquid bridge and system 有权
标题翻译：液桥和系统
公开(公告)号：US08298833B2
公开(公告)日：2012-10-30
申请号：US12092261
申请日：2007-02-07
申请人： Mark Davies , Tara Dalton , Kieran Curran
发明人： Mark Davies , Tara Dalton , Kieran Curran
IPC分类号： G01N1/10
CPC分类号： B01L3/502784 , B01F13/0071 , B01F15/0201 , B01F15/0232 , B01F2215/0037 , B01L3/5025 , B01L3/502715 , B01L3/502746 , B01L7/525 , B01L2200/0673 , B01L2300/0829 , B01L2300/0838 , B01L2400/0478 , B01L2400/0487 , B01L2400/0633 , B01L2400/082 , C12Q1/686 , F17D1/08 , Y10T137/87676 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.
摘要翻译：桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33）和用于硅油的室（34）。油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。室内的油通过分离反应器液滴的油不断补充。这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

6. 发明申请

US20100092987A1 MICROFLUIDIC ANALYSIS SYSTEM 审中-公开
标题翻译：微流感分析系统
公开(公告)号：US20100092987A1
公开(公告)日：2010-04-15
申请号：US12617286
申请日：2009-11-12
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/686 , B01F5/0057 , B01F13/0059 , B01L3/50273 , B01L3/502776 , B01L3/502784 , B01L7/525 , B01L2200/0636 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0861 , B01L2300/087 , B01L2300/185 , B01L2400/0409 , B01L2400/0487 , G01N35/08 , G01N35/1095 , G01N2035/00514
摘要： A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.
摘要翻译：微流体分析系统（1）对生物样品进行聚合酶链反应（PCR）分析。在离心机（6）中，将样品分离成DNA和RNA成分。涡流通过由粘性阻力影响循环的硅油主载流体的相反流动而产生。生物样品离开包围在主载体流体中的离心机。这被流量控制器（7）泵送到热级（9）。热级（9）具有多个微流体装置（70），每个微流体装置（70）各自具有热区（71,72,73），其中生物样品通过向热载体流体和主载体流体的热传导而被加热或冷却。因此，载体流体包裹样品，控制其流速，并控制其温度，而不需要微量级的移动部件。

7. 发明申请

US20100041055A1 NOVEL GENE NORMALIZATION METHODS 审中-公开
标题翻译：新基因正常化方法
公开(公告)号：US20100041055A1
公开(公告)日：2010-02-18
申请号：US12539773
申请日：2009-08-12
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6886 , C12Q2545/101
摘要： Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene.
摘要翻译：相对于内源性对照基因的基因表达的测量在样品之间容易发生过度的变异，甚至复制。本公开提供了通过将基因表达水平与表达水平测定的基因组中最高表达基因的基因表达水平相比较而不是保存基因来使基因表达水平正常化的方法。

8. 发明授权

US08968659B2 Sample dispensing 有权
标题翻译：样品分配
公开(公告)号：US08968659B2
公开(公告)日：2015-03-03
申请号：US12683882
申请日：2010-01-07
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： G01N1/18 , G01N1/00 , G01N33/00 , C40B60/14 , B01F5/00 , B01F13/00 , B01L3/00 , B01L7/00 , G01N35/08 , G01N35/10 , G01N35/00
CPC分类号： B01F5/0057 , B01F13/0059 , B01F13/0071 , B01L3/50273 , B01L7/525 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2300/0861 , B01L2300/087 , B01L2300/185 , B01L2400/0409 , B01L2400/0487 , G01N35/08 , G01N35/1095 , G01N2035/00514
摘要： The present invention generally relates to systems and methods for mixing and dispensing a sample droplet from a microfluidic system, such as a liquid bridge system. In certain embodiments, the invention provides systems for mixing and dispensing sample droplets, including a sample acquisition stage, a device for mixing sample droplets to form sample droplets wrapped in an immiscible carrier fluid, a dispensing port, and at least one channel connecting the stage, the droplet mixing device, and the port, in which the system is configured to establish a siphoning effect for dispensing the droplets.
摘要翻译：本发明一般涉及用于从微流体系统（例如液桥系统）混合和分配样品液滴的系统和方法。在某些实施方案中，本发明提供了用于混合和分配样品液滴的系统，包括样品采集阶段，用于混合样品液滴以形成包裹在不混溶载体流体中的样品液滴的装置，分配端口和连接该阶段，液滴混合装置和端口，其中系统被配置成建立用于分配液滴的虹吸效应。

9. 发明授权

US08550503B2 Microfluidic connector 失效
标题翻译：微流体连接器
公开(公告)号：US08550503B2
公开(公告)日：2013-10-08
申请号：US12443303
申请日：2007-09-27
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： F16L39/04
CPC分类号： F16L41/18 , B01L3/5027 , B01L3/5635 , B01L3/565 , B01L2200/027 , F16L39/00 , Y10T137/0318
摘要： A microfluidic connector (1) comprises an enclosure (6, 7), a fluidic inlet port (2) and a fluidic outlet port (3), in the enclosure, in which the inlet and outlet ports (2, 3) are movable with respect to each other, for example, mutual spacing between the inlet and outlet ports (2, 3) is variable. A port (2) is in a fixed part (6) of the enclosure, and another port (3) is in a part (7) of the enclosure which slides with respect to the fixed part. There may be multiple inlet ports (22, 23) and/or multiple outlet ports (24, 25). Also, there may be an auxiliary port (45) for introduction of fluid into the enclosure (47, 48) or removal of fluid from the enclosure.
摘要翻译：微流体连接器（1）包括外壳中的外壳（6,7），流体入口端口（2）和流体出口端口（3），入口端口和出口端口（2,3）可通过外壳彼此相对，例如，入口端口（3）之间的相互间隔是可变的。端口（2）位于外壳的固定部分（6）中，另一个端口（3）位于外壳的相对于固定部分滑动的部分（7）中。可以有多个入口端口（22,23）和/或多个出口端口（24,25）。而且，可以存在用于将流体引入外壳（47,48）或从外壳移除流体的辅助端口（45）。

10. 发明申请

US20100216128A1 METHODS FOR ANALYZING AGRICULTURAL AND ENVIRONMENTAL SAMPLES 有权
标题翻译：分析农业和环境样品的方法
公开(公告)号：US20100216128A1
公开(公告)日：2010-08-26
申请号：US12469339
申请日：2009-05-20
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68 , C12Q1/02 , G01N33/566 , G01N33/92 , G01N33/00
CPC分类号： G01N1/38 , B01F13/0071 , B01F15/0201 , B01F15/0232 , B01L3/502784 , B01L3/5088 , B01L2200/10 , B01L2300/0816 , B01L2300/0864 , B01L2300/165 , G01N33/5308 , G01N2035/00237
摘要： The present invention generally relates methods for analyzing agricultural and/or environmental samples using liquid bridges. In certain embodiments, the invention provides a method for analyzing an agricultural sample for a desired trait including obtaining a gene or gene product from an agricultural sample, in which the gene or gene product is in a first fluid; providing a liquid bridge for mixing the gene or gene product with at least one reagent to form a mixed droplet that is wrapped in an immiscible second fluid; and analyzing the mixed droplet to detect a desired trait of the agricultural sample.
摘要翻译：本发明一般涉及使用液体桥分析农业和/或环境样品的方法。在某些实施方案中，本发明提供了一种用于分析所需性状的农业样品的方法，包括从农业样品获得基因或基因产物，其中所述基因或基因产物处于第一种流体中; 提供用于将基因或基因产物与至少一种试剂混合以形成被包裹在不混溶的第二流体中的混合液滴的液体桥; 并分析混合液滴以检测农业样品的期望性状。

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