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1. 发明专利

JP2020089368A トランスジェニック生物における多不飽和脂肪酸の製造方法审中-公开
公开(公告)号：JP2020089368A
公开(公告)日：2020-06-11
申请号：JP2020000076
申请日：2020-01-06
申请人： BASF PLANT SCIENCE GMBH
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/53 , A01H5/00 , A01K67/027 , A23D9/00 , A23K20/158 , A23L33/105 , A23L33/115 , A61K8/36 , A61K8/97 , A61K31/201 , A61K31/202 , A61K36/00 , A61P3/06 , A61P9/00 , A61P9/04 , A61P9/12 , A61P19/02 , A61P29/00 , A61P37/02 , A61Q1/00 , A61Q19/00 , C11B1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/10 , C12N15/63 , C12N15/82 , C12P7/64
摘要：【課題】生物における多不飽和脂肪酸の製造方法、及び、長鎖多不飽和脂肪酸の含量が上昇した油及び/又はトリアシルグリセリドの製造方法の提供。【解決手段】有利には、タラッシオシラ、ユーグレナ又はオストレオコッカスに由来する、Δ5—エロンガーゼ活性、さらにはΔ6—デサチュラーゼ、Δ5—デサチュラーゼ、Δ4—デサチュラーゼ、Δ12—デサチュラーゼ及び/又はΔ6—エロンガーゼ活性を有するポリペプチドをコードする核酸を生物に導入することによる、生物における多不飽和脂肪酸の製造方法。トランスジェニック生物は、好ましくは、トランスジェニック植物又はトランスジェニック微生物である。【選択図】なし

2. 发明专利

JP2014128269A Method for production of multiply-unsaturated fatty acids in transgenic organisms 有权
标题翻译：在转基因生物中生产多余不饱和脂肪酸的方法
公开(公告)号：JP2014128269A
公开(公告)日：2014-07-10
申请号：JP2013270709
申请日：2013-12-27
申请人： Basf Plant Science Gmbh , ビーエーエスエフプラントサイエンスゲーエムベーハー
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/09 , A01H5/00 , A01K67/027 , A23D9/00 , A23K1/16 , A23L1/30 , A61K8/36 , A61K8/37 , A61K31/202 , A61K31/232 , A61P3/06 , C11B1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for producing multiply-unsaturated fatty acids in an organism into which nucleic acids have been introduced, the nucleic acids encoding polypeptides with Δ-5 elongase activity.SOLUTION: The method for the production of an oil and/or triacylglyceride which has increased content of long chain multiply-unsaturated fatty acids employs a vector with an isolated nucleic acid sequence encoding a polypeptide having Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity derived from Thalassiosira, Euglena, or Ostreococcus, and enzymes of an introduced transgenic organism: a microorganism, a nonhuman animal or a plant.
摘要翻译：要解决的问题：提供一种在已经引入了核酸的生物体中生产多不饱和脂肪酸的方法，编码具有＆Dgr-5延伸酶活性的多肽的核酸。解决方案：生产油的方法具有增加的长链多不饱和脂肪酸含量的甘油三酯和/或三酰基甘油酯使用具有分离的核酸序列的载体，所述分离的核酸序列编码具有＆Dgr; -6去饱和酶，＆Dgr; -5去饱和酶，Dgr-4去饱和酶和/ 衍生自Thalassiosira，Euglena或Ostreococcus的延长酶活性和引入的转基因生物的酶：微生物，非人动物或植物。

3. 发明专利

JP2011087578A Method for production of multiple-unsaturated fatty acid in transgenic organism 审中-公开
标题翻译：在转基因生物中生产多种不饱和脂肪酸的方法
公开(公告)号：JP2011087578A
公开(公告)日：2011-05-06
申请号：JP2010232883
申请日：2010-10-15
申请人： Basf Plant Science Gmbh , ビーエーエスエフプラントサイエンスゲーエムベーハー
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/09 , A01H5/00 , A01K67/027 , A23D9/00 , A23L1/30 , A61K8/36 , A61K8/37 , A61K31/202 , A61K31/232 , A61P3/06 , C11B1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a multiple-unsaturated fatty acid in an organism, into which a nucleic acid coding for a polypeptide having Δ-5 elongase activity is introduced. SOLUTION: There are provided a nucleic acid sequence coding for Δ-6 desaturase, Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity and derived from Thalassiosira, Euglena or Ostreococcus, and a genetic construction containing the nucleic acid sequence. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供在生物体中制备多不饱和脂肪酸的方法，其中引入编码具有Δ-5延伸酶活性的多肽的核酸。解决方案：提供了编码Δ-6去饱和酶，Δ-5去饱和酶，Δ-4去饱和酶和/或Δ-6延伸酶活性的核酸序列，并且衍生自海水藻，欧洲淋巴细胞球菌或油球藻，以及含有核酸序列。版权所有（C）2011，JPO＆INPIT

4. 发明申请

WO2010066703A3 DESATURASES AND PROCESS FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：在转基因生物中生产聚氨酯脂肪酸的苦味和过程
公开(公告)号：WO2010066703A3
公开(公告)日：2010-09-23
申请号：PCT/EP2009066569
申请日：2009-12-08
申请人： BASF PLANT SCIENCE GMBH , SENGER TORALF , BAUER JOERG , NAPIER JOHNATHAN A
发明人： SENGER TORALF , BAUER JOERG , NAPIER JOHNATHAN A
IPC分类号： C12N15/82 , A01H5/00 , C12N9/02 , C12N15/53 , C12P7/64
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , C12P7/6481 , C12Y114/19001
摘要： The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recom bi nant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.
摘要翻译：本发明涉及来自嗜球菌（Cocchioiobolus heterostrophus）C5，蓝藻（Cyanothece sp。）的多核苷酸 CCY0110，Acycina霉菌和Hyaloperonospora parasitica，其代码为去饱和酶，可用于多不饱和脂肪酸的重复生产。本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。本发明还涉及针对根据本发明的多肽的抗体。最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。

5. 发明申请

WO2007017419A3 METHOD FOR PRODUCING ARACHIDONIC ACID AND/OR EICOSAPENTAENOIC ACID IN USEFUL TRANSGENIC PLANTS 审中-公开
标题翻译：在转基因植物中生产阿拉酸和/或艾考酸的方法
公开(公告)号：WO2007017419A3
公开(公告)日：2007-05-10
申请号：PCT/EP2006064922
申请日：2006-08-01
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI , TRUKSA MARTIN
发明人： CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI , TRUKSA MARTIN
IPC分类号： C12N15/82 , C07K14/415 , C11B1/02 , C12N9/02
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A61K8/361 , A61Q19/00 , C12N9/0083 , C12N9/1029
摘要： The invention relates to a method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants, according to which nucleic acids, which encode polypeptides with ?-6-desaturase activity, ?-6-elongase activity or ?-5-desaturase activity, are introduced into the plants. As an added advantage, a gene that encodes a ?-3-desaturase is expressed in the useful plants. In an additional embodiment of the method, additional nucleic acid sequences, which encode polypeptides involved in the biosynthesis of the fatty acid or lipid metabolism, can be expressed in the plants. Particularly advantageous nucleic acid sequences are those that encode ?-8-desaturase activity, ?-12-desaturase activity, ?-15-desaturase activity, ?-4-desaturase activity, ?-9-elongase activity and/or ?-5-elongase activity. The invention also relates to the use of the oils, lipids and/or fatty acids that have been produced by said method in animal feed or foodstuffs, cosmetics or pharmaceutical products.
摘要翻译：本发明涉及用于生产花生四烯酸和/或通过核酸的转基因作物植物的二十碳五烯酸的方法被引入到编码的多肽具有其中植物？-6去饱和酶，？-6-延伸酶或？-5去饱和酶活性，有利的是，编码α-3-去饱和酶的基因还在农作物中表达。在该方法的另一个有利的实施方案中，可以在植物中表达编码脂肪酸或脂质代谢的生物合成的多肽的其他核酸序列。这是编码的8去饱和酶特别有利的核酸序列 - “ - ？-9-延伸12去饱和酶，-15去饱和酶，-4-去饱和酶，和/或???? - 编码5-延伸酶活性。本发明的另一部分涉及使用的在饲料或食品，化妆品或药品中的本发明方法的油，脂质和/或脂肪酸的制造。

6. 发明申请

WO03095655A2 METHODS FOR INCREASING OIL CONTENT IN PLANTS 审中-公开
标题翻译：方法提高含油量在植物
公开(公告)号：WO03095655A2
公开(公告)日：2003-11-20
申请号：PCT/EP0304711
申请日：2003-05-06
申请人： BASF PLANT SCIENCE GMBH , RENZ ANDREAS , BAUER JOERG , STITT NIGEL MARC , ZRENNER RITA MARIA , GEIGENBERGER PETER , VIGEOLAS HELENE
发明人： RENZ ANDREAS , BAUER JOERG , STITT NIGEL MARC , ZRENNER RITA MARIA , GEIGENBERGER PETER , VIGEOLAS HELENE
IPC分类号： C12N9/04 , C12N15/82
CPC分类号： C12N9/0006 , C12N15/8247
摘要： The invention relates to methods for increasing the oil content in plants, preferably in the seeds of plants, by expression of glycerol-3-phosphatdehydrogenases (G3PDH) from yeast, preferably from Saccharomyces cerevisiae. The invention also relates to expression constructs for the expression of G3PDH yeast in plants, preferably in the seeds of plants, transgenic plants expressing G3PDH, and to the use of said transgenic plants in the production of foodstuffs, feed, seeds, pharmaceuticals or fine chemicals, especially in the production of oils.
摘要翻译：本发明涉及一种方法，用于通过从酵母甘油醛-3-磷酸脱氢酶（G3PDH），优选酿酒酵母的表达增加在植物中的油含量，优选在植物种子。本发明还涉及表达构建酵母G3PDH在植物中的表达，优选地在植物种子，表达酵母G3PDH的转基因植物，和使用所述转基因植物用于生产食品，饲料，种子，药物或者精细化学品的，特别是用于生产油的，

7. 发明申请

WO2010023202A3 NUCLEIC ACIDS ENCODING DESATURASES AND MODIFIED PLANT OIL 审中-公开
标题翻译：核酸编码和改性植物油
公开(公告)号：WO2010023202A3
公开(公告)日：2010-06-24
申请号：PCT/EP2009060923
申请日：2009-08-25
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP , BAUER JOERG , SENGER TORALF , ZANK THORSTEN , QIU XIAO , WU GUOHAI
发明人： BAUER JOERG , SENGER TORALF , ZANK THORSTEN , QIU XIAO , WU GUOHAI
IPC分类号： C12N15/82 , C12N9/00
CPC分类号： C12N15/8247 , C12N5/14 , C12N9/00 , C12N9/0004
摘要： The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.
摘要翻译：本发明涉及来自Drechslera tritici-repentis，Cylindorcarpon herteronema，Diploida natalensis，Stagonospora nodorum，Microdochium nivalae和Periplaneta americana的核酸。本发明还涉及个体编码序列和由这些序列与其它序列组合编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法，以及花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸的生产酸在植物中。

8. 发明申请

WO2009016202A3 DESATURASES AND METHODS FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：去饱和酶和过程在转基因生物生产多不饱和脂肪酸
公开(公告)号：WO2009016202A3
公开(公告)日：2009-06-18
申请号：PCT/EP2008059999
申请日：2008-07-30
申请人： BASF PLANT SCIENCE GMBH , BAUER JOERG , SENGER TORALF
发明人： BAUER JOERG , SENGER TORALF
IPC分类号： C12Q1/68 , C12N15/82
CPC分类号： C12N15/8247 , C12N9/0083
摘要： The invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruben, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale that code desaturases and that can be used for the recombinant production of polyunsaturated fatty acids. The invention also relates to the vectors, host cells and transgenic non-human organisms that contain said inventive polynucleotides, and also the polypeptides coded by said polynucleotides. The invention further relates to antibodies against the inventive polypeptides. Finally, the invention relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to the use thereof as medicments, cosmetics, food, animal food, preferably fish food or food supplements.
摘要翻译：本发明涉及Helobdella罗布斯塔，双色蜡蘑，Lottia巨，Microcoleus chthonoplastes，Monosiga brevicollis，斐济球腔菌，Mycospaerella蛛，福氏耐格里坑，Nectria haematococca，Nematostella vectensis，须霉属blakesleeanus，木霉属resii，小立碗藓，Postia胎盘，江南卷柏的多核苷酸和微结节nivale编码去饱和酶和可用于重组生产多不饱和脂肪酸。此外，本发明涉及载体，宿主细胞和包含本发明的转基因非人生物的多核苷酸，以及由所述多核苷酸的多肽编码的蛋白质。本发明还涉及抗体与本发明的多肽。最后，本发明涉及一种制造方法，所述多不饱和脂肪酸和油，脂类和脂肪酸组合物和它们作为药物，化妆品，食品，饲料，优选鱼饲料或食品补充剂。

9. 发明申请

WO2006069936A3 METHOD FOR PRODUCING POLYUNSATURATED LONG-CHAIN FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：过程在转基因生物生产多种不饱和长链脂肪酸
公开(公告)号：WO2006069936A3
公开(公告)日：2007-03-22
申请号：PCT/EP2005056957
申请日：2005-12-20
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , HEINZ ERNST , ABBADI AMINE , KIRSCH JELENA
发明人： CIRPUS PETRA , BAUER JOERG , HEINZ ERNST , ABBADI AMINE , KIRSCH JELENA
IPC分类号： C12N9/10 , A01K67/027 , C12N5/10 , C12N15/54
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译：本发明涉及一种用于生产在由核酸的生物体的多不饱和脂肪酸的方法被引入到生物体中，其用于与酰基辅酶A的多肽代码：溶血磷脂酰基转移酶活性。有利地，这些核酸序列可以在转基因有机体与其他核酸的脂肪酸或脂质的多肽的代码序列表示任选在一起。本发明还涉及含有包含上述核酸序列，核酸构建体和/或载体中的本发明和/或所述核酸构建体和转基因生物的核酸序列的本发明的核酸序列，含有本发明的核酸序列的核酸构建体，载体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。

10. 发明申请

WO2005083093A3 METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC PLANTS 审中-公开
标题翻译： PROCESS转基因植物中多产多不饱和脂肪酸
公开(公告)号：WO2005083093A3
公开(公告)日：2006-02-16
申请号：PCT/EP2005001863
申请日：2005-02-23
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI
发明人： CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI
IPC分类号： C11B1/02 , C12N9/02 , C12N9/10 , A01K67/027
CPC分类号： C12N15/82 , C12N15/8247
摘要： The invention relates to a method for producing polyunsaturated fatty acids in seeds of transgenic plants. According to said method, nucleic acids, coding for polypeptides with a ?-3-desaturase, ?-12-desaturase, ?-6-desaturase, ?-6-elongase, ?-5-desaturase, ?-5-elongase and/or ?-4-desaturase activity, preferably for polypeptides with a ?-6-desaturase, ?-6-elongase and ?-5-desaturase activity, are introduced into the organism. The nucleic acid sequences are represented by SEQ ID NO: 11, SEQ ID NO: 27, SEQ ID NO: 193, SEQ ID NO: 197, SEQ ID NO: 199 and SEQ ID NO: 201. Advantageously, said nucleic acid sequences can be expressed in the organism optionally together with other nucleic acid sequences that code for polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Nucleic acid sequences coding for a ?-6-desaturase, ?-5-desaturase, ?-4-desaturase, ?-1 2-desaturase and/or ?-6-elongase activity are especially advantageous. Advantageously, said desaturases and elongases originate from thalassiosira, euglena or ostreococcus. The invention also relates to a method for producing oils and/or triacylglycerides with an increased content of long-chain polyunsaturated fatty acids. In a preferred embodiment, the invention also relates to a method for producing arachidonic acid, eicosapentaenic acid or docosahexaenic acid, and to a method for producing triglycerides with an increased content of unsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, in transgenic plants, preferably in seeds of the transgenic plants. The invention further relates to the production of a transgenic plant with an increased content of polyunsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, based on the expression of the elongases and desaturases used in the inventive method. The invention also relates to recombinant nucleic acids molecules containing, together or individually, nucleic acid sequences coding for the polypeptides with a ?-6-desaturase, ?-6-elongase, ?-5-desaturase and ?-5-elongase activity, and transgenic plants containing said recombinant nucleic acid molecules. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids, and to the use of the same.
摘要翻译：本发明涉及一种用于生产由核酸的转基因植物的种子中的多不饱和脂肪酸的方法被引入到生物体中，其与β-3去饱和酶，？-12-去饱和酶，？-6去饱和酶编码多肽，？-6-延伸,? -5去饱和酶，？-5-延伸酶和/或Δ4-去饱和酶优选的多肽具有？-6去饱和酶，？-6延伸酶和？-5- 脱氢酶活性。的核酸序列示于SEQ ID NO：11，SEQ ID NO：27，SEQ ID NO：193，SEQ ID NO：197，SEQ ID NO：199和SEQ ID NO：所示第二百○一序列有利地，这些核酸序列可以在生物体中，任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。特别有利的是编码一个？-6去饱和酶中，α-1 2去饱和酶-6-延伸-5去饱和酶，？4去饱和酶，？和/或核酸序列？有利的是，这些去饱和酶和延伸酶海链藻，裸藻或Ostreococcus。此外，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。本发明中的优选实施例还涉及一种用于生产花生四烯酸，二十碳五烯酸或二十二碳六酸和用于生产甘油三酯的与不饱和脂肪酸含量增加的处理，特别是花生四烯酸，二十碳五烯酸和/或二十碳六烯酸的，有利的是在转基因植物中的转基因的种子植物。本发明涉及生产具有多不饱和脂肪酸，特别是花生四烯酸，二十碳五烯酸和/或二十碳六烯酸，含量增加的转基因植物由于延伸酶的表达和在本发明的去饱和酶处理中使用。本发明还涉及含有核酸序列，其的重组核酸分子编码的多肽具有？-6去饱和酶，？-6-延伸,? -5去饱和酶和β-5-延伸酶，联合或单独，以及含有上述的重组核酸分子的转基因植物。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

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