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    • 6. 发明申请
    • METHOD FOR THE PRODUCTION OF MULTIPLY-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS
    • 用于生产不饱和脂肪酸的转基因多
    • WO2005012316A3
    • 2005-10-06
    • PCT/EP2004007957
    • 2004-07-16
    • BASF PLANT SCIENCE GMBHZANK THORSTENBAUER JOERGCIRPUS PETRAABBADI AMINEHEINZ ERNSTQIU XIAOVRINTEN PATRICIASPERLING PETRADOMERGUE FREDERICMEYER ASTRIDKIRSCH JELENA
    • ZANK THORSTENBAUER JOERGCIRPUS PETRAABBADI AMINEHEINZ ERNSTQIU XIAOVRINTEN PATRICIASPERLING PETRADOMERGUE FREDERICMEYER ASTRIDKIRSCH JELENA
    • A23D9/00C11B1/02C12N9/02C12N9/10C12N15/82C12P7/64A01K67/027
    • C12N15/8247A23D9/00A23D9/02A23L33/115A23V2002/00C11B1/00C12N9/0071C12N9/0083C12N9/0093C12N9/1029C12P7/6409C12P7/6427C12P7/6463C12P7/6472C12Y114/19C12Y114/19003C12Y203/01199Y02P20/52
    • The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Delta-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Delta-6 desaturase, a Delta-5 desaturase, Delta-4 desaturase and/or Delta-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated omega-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Delta-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of omega-3 fatty acids, oils or lipids with omega-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with omega-3 desaturases, for example a omega-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a omega-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
    • 本发明涉及一种用于通过核酸的生物体制备的多个unge饱和脂肪酸的方法被引入到生物体中,这对于多肽与Δ-5-延伸酶活性的代码。 有利地,这些核酸序列可以在生物体中,任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。 特别有利的是编码Δ-4-Desaturaseund /或Δ-6去饱和酶,Δ-5去饱和酶的Δ-6延长酶的活性的核酸序列。 有利的是,这些去饱和酶和延伸酶海链藻,裸藻或Ostreococcus。 此外,本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。 此外,在一个优选的实施方案中,本发明涉及用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的过程中,具有超过三个双键尤其ω-3脂肪酸。 本发明涉及产生转基因生物,优选的转基因植物或转基因微生物,其具有具有由于延伸酶的表达ω-3双键的不饱和的ω-3脂肪酸,油或脂质的含量增加,并在本发明中连接有利的过程中去饱和酶使用 与ω-3去饱和酶,例如, 从家庭Pythiaceae的真菌,如属疫ω-3去饱和酶,例如属和种致病疫霉或从藻类ω-3去饱和酶诸如家庭绿枝藻纲例如的 属Ostreococcus具体的属和种Ostreococcus桃源或硅藻如海链藻属特别是属和种海链pseudonana。 本发明还涉及含有本发明核酸序列的核酸序列,核酸构建体,载体和有机体,将含有包含上述核酸序列,核酸构建体和/或载体的核酸序列和/或核酸构建体,和转基因生物体的载体。 本发明的另一部分涉及通过本发明和它们的使用过程中产生的油,脂质和/或脂肪酸。 本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。
    • 7. 发明申请
    • METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC PLANTS
    • PROCESS转基因植物中多产多不饱和脂肪酸
    • WO2005083093A3
    • 2006-02-16
    • PCT/EP2005001863
    • 2005-02-23
    • BASF PLANT SCIENCE GMBHCIRPUS PETRABAUER JOERGQIU XIAOWU GUOHAIDATLA NAGAMANI
    • CIRPUS PETRABAUER JOERGQIU XIAOWU GUOHAIDATLA NAGAMANI
    • C11B1/02C12N9/02C12N9/10A01K67/027
    • C12N15/82C12N15/8247
    • The invention relates to a method for producing polyunsaturated fatty acids in seeds of transgenic plants. According to said method, nucleic acids, coding for polypeptides with a ?-3-desaturase, ?-12-desaturase, ?-6-desaturase, ?-6-elongase, ?-5-desaturase, ?-5-elongase and/or ?-4-desaturase activity, preferably for polypeptides with a ?-6-desaturase, ?-6-elongase and ?-5-desaturase activity, are introduced into the organism. The nucleic acid sequences are represented by SEQ ID NO: 11, SEQ ID NO: 27, SEQ ID NO: 193, SEQ ID NO: 197, SEQ ID NO: 199 and SEQ ID NO: 201. Advantageously, said nucleic acid sequences can be expressed in the organism optionally together with other nucleic acid sequences that code for polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Nucleic acid sequences coding for a ?-6-desaturase, ?-5-desaturase, ?-4-desaturase, ?-1 2-desaturase and/or ?-6-elongase activity are especially advantageous. Advantageously, said desaturases and elongases originate from thalassiosira, euglena or ostreococcus. The invention also relates to a method for producing oils and/or triacylglycerides with an increased content of long-chain polyunsaturated fatty acids. In a preferred embodiment, the invention also relates to a method for producing arachidonic acid, eicosapentaenic acid or docosahexaenic acid, and to a method for producing triglycerides with an increased content of unsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, in transgenic plants, preferably in seeds of the transgenic plants. The invention further relates to the production of a transgenic plant with an increased content of polyunsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, based on the expression of the elongases and desaturases used in the inventive method. The invention also relates to recombinant nucleic acids molecules containing, together or individually, nucleic acid sequences coding for the polypeptides with a ?-6-desaturase, ?-6-elongase, ?-5-desaturase and ?-5-elongase activity, and transgenic plants containing said recombinant nucleic acid molecules. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids, and to the use of the same.
    • 本发明涉及一种用于生产由核酸的转基因植物的种子中的多不饱和脂肪酸的方法被引入到生物体中,其与β-3去饱和酶,?-12-去饱和酶,?-6去饱和酶编码多肽, ?-6-延伸,? -5去饱和酶,?-5-延伸酶和/或Δ4-去饱和酶优选的多肽具有?-6去饱和酶,?-6延伸酶和?-5- 脱氢酶活性。 的核酸序列示于SEQ ID NO:11,SEQ ID NO:27,SEQ ID NO:193,SEQ ID NO:197,SEQ ID NO:199和SEQ ID NO:所示第二百○一序列 有利地,这些核酸序列可以在生物体中,任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。 特别有利的是编码一个?-6去饱和酶中,α-1 2去饱和酶-6-延伸-5去饱和酶,?4去饱和酶,?和/或核酸序列? 有利的是,这些去饱和酶和延伸酶海链藻,裸藻或Ostreococcus。 此外,本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。 本发明中的优选实施例还涉及一种用于生产花生四烯酸,二十碳五烯酸或二十二碳六酸和用于生产甘油三酯的与不饱和脂肪酸含量增加的处理,特别是花生四烯酸,二十碳五烯酸和/或二十碳六烯酸的,有利的是在转基因植物中的转基因的种子 植物。 本发明涉及生产具有多不饱和脂肪酸,特别是花生四烯酸,二十碳五烯酸和/或二十碳六烯酸,含量增加的转基因植物由于延伸酶的表达和在本发明的去饱和酶处理中使用。 本发明还涉及含有核酸序列,其的重组核酸分子编码的多肽具有?-6去饱和酶,?-6-延伸,? -5去饱和酶和β-5-延伸酶,联合或单独,以及 含有上述的重组核酸分子的转基因植物。 本发明的另一部分涉及通过本发明和它们的使用过程中产生的油,脂质和/或脂肪酸。 本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。
    • 9. 发明申请
    • DESATURASES AND METHODS FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS
    • 去饱和酶和过程在转基因生物生产多不饱和脂肪酸
    • WO2009016202A3
    • 2009-06-18
    • PCT/EP2008059999
    • 2008-07-30
    • BASF PLANT SCIENCE GMBHBAUER JOERGSENGER TORALF
    • BAUER JOERGSENGER TORALF
    • C12Q1/68C12N15/82
    • C12N15/8247C12N9/0083
    • The invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruben, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale that code desaturases and that can be used for the recombinant production of polyunsaturated fatty acids. The invention also relates to the vectors, host cells and transgenic non-human organisms that contain said inventive polynucleotides, and also the polypeptides coded by said polynucleotides. The invention further relates to antibodies against the inventive polypeptides. Finally, the invention relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to the use thereof as medicments, cosmetics, food, animal food, preferably fish food or food supplements.
    • 本发明涉及Helobdella罗布斯塔,双色蜡蘑,Lottia巨,Microcoleus chthonoplastes,Monosiga brevicollis,斐济球腔菌,Mycospaerella蛛,福氏耐格里坑,Nectria haematococca,Nematostella vectensis,须霉属blakesleeanus,木霉属resii,小立碗藓,Postia胎盘,江南卷柏的多核苷酸 和微结节nivale编码去饱和酶和可用于重组生产多不饱和脂肪酸。 此外,本发明涉及载体,宿主细胞和包含本发明的转基因非人生物的多核苷酸,以及由所述多核苷酸的多肽编码的蛋白质。 本发明还涉及抗体与本发明的多肽。 最后,本发明涉及一种制造方法,所述多不饱和脂肪酸和油,脂类和脂肪酸组合物和它们作为药物,化妆品,食品,饲料,优选鱼饲料或食品补充剂。