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1. 发明专利

JP2014128269A Method for production of multiply-unsaturated fatty acids in transgenic organisms 有权
标题翻译：在转基因生物中生产多余不饱和脂肪酸的方法
公开(公告)号：JP2014128269A
公开(公告)日：2014-07-10
申请号：JP2013270709
申请日：2013-12-27
申请人： Basf Plant Science Gmbh , ビーエーエスエフプラントサイエンスゲーエムベーハー
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/09 , A01H5/00 , A01K67/027 , A23D9/00 , A23K1/16 , A23L1/30 , A61K8/36 , A61K8/37 , A61K31/202 , A61K31/232 , A61P3/06 , C11B1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for producing multiply-unsaturated fatty acids in an organism into which nucleic acids have been introduced, the nucleic acids encoding polypeptides with Δ-5 elongase activity.SOLUTION: The method for the production of an oil and/or triacylglyceride which has increased content of long chain multiply-unsaturated fatty acids employs a vector with an isolated nucleic acid sequence encoding a polypeptide having Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity derived from Thalassiosira, Euglena, or Ostreococcus, and enzymes of an introduced transgenic organism: a microorganism, a nonhuman animal or a plant.
摘要翻译：要解决的问题：提供一种在已经引入了核酸的生物体中生产多不饱和脂肪酸的方法，编码具有＆Dgr-5延伸酶活性的多肽的核酸。解决方案：生产油的方法具有增加的长链多不饱和脂肪酸含量的甘油三酯和/或三酰基甘油酯使用具有分离的核酸序列的载体，所述分离的核酸序列编码具有＆Dgr; -6去饱和酶，＆Dgr; -5去饱和酶，Dgr-4去饱和酶和/ 衍生自Thalassiosira，Euglena或Ostreococcus的延长酶活性和引入的转基因生物的酶：微生物，非人动物或植物。

2. 发明专利

JP2011087578A Method for production of multiple-unsaturated fatty acid in transgenic organism 审中-公开
标题翻译：在转基因生物中生产多种不饱和脂肪酸的方法
公开(公告)号：JP2011087578A
公开(公告)日：2011-05-06
申请号：JP2010232883
申请日：2010-10-15
申请人： Basf Plant Science Gmbh , ビーエーエスエフプラントサイエンスゲーエムベーハー
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/09 , A01H5/00 , A01K67/027 , A23D9/00 , A23L1/30 , A61K8/36 , A61K8/37 , A61K31/202 , A61K31/232 , A61P3/06 , C11B1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a multiple-unsaturated fatty acid in an organism, into which a nucleic acid coding for a polypeptide having Δ-5 elongase activity is introduced. SOLUTION: There are provided a nucleic acid sequence coding for Δ-6 desaturase, Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity and derived from Thalassiosira, Euglena or Ostreococcus, and a genetic construction containing the nucleic acid sequence. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供在生物体中制备多不饱和脂肪酸的方法，其中引入编码具有Δ-5延伸酶活性的多肽的核酸。解决方案：提供了编码Δ-6去饱和酶，Δ-5去饱和酶，Δ-4去饱和酶和/或Δ-6延伸酶活性的核酸序列，并且衍生自海水藻，欧洲淋巴细胞球菌或油球藻，以及含有核酸序列。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2020089368A トランスジェニック生物における多不飽和脂肪酸の製造方法审中-公开
公开(公告)号：JP2020089368A
公开(公告)日：2020-06-11
申请号：JP2020000076
申请日：2020-01-06
申请人： BASF PLANT SCIENCE GMBH
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： C12N15/53 , A01H5/00 , A01K67/027 , A23D9/00 , A23K20/158 , A23L33/105 , A23L33/115 , A61K8/36 , A61K8/97 , A61K31/201 , A61K31/202 , A61K36/00 , A61P3/06 , A61P9/00 , A61P9/04 , A61P9/12 , A61P19/02 , A61P29/00 , A61P37/02 , A61Q1/00 , A61Q19/00 , C11B1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/10 , C12N15/63 , C12N15/82 , C12P7/64
摘要：【課題】生物における多不飽和脂肪酸の製造方法、及び、長鎖多不飽和脂肪酸の含量が上昇した油及び/又はトリアシルグリセリドの製造方法の提供。【解決手段】有利には、タラッシオシラ、ユーグレナ又はオストレオコッカスに由来する、Δ5—エロンガーゼ活性、さらにはΔ6—デサチュラーゼ、Δ5—デサチュラーゼ、Δ4—デサチュラーゼ、Δ12—デサチュラーゼ及び/又はΔ6—エロンガーゼ活性を有するポリペプチドをコードする核酸を生物に導入することによる、生物における多不飽和脂肪酸の製造方法。トランスジェニック生物は、好ましくは、トランスジェニック植物又はトランスジェニック微生物である。【選択図】なし

4. 发明申请

WO2006069936A3 METHOD FOR PRODUCING POLYUNSATURATED LONG-CHAIN FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：过程在转基因生物生产多种不饱和长链脂肪酸
公开(公告)号：WO2006069936A3
公开(公告)日：2007-03-22
申请号：PCT/EP2005056957
申请日：2005-12-20
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , HEINZ ERNST , ABBADI AMINE , KIRSCH JELENA
发明人： CIRPUS PETRA , BAUER JOERG , HEINZ ERNST , ABBADI AMINE , KIRSCH JELENA
IPC分类号： C12N9/10 , A01K67/027 , C12N5/10 , C12N15/54
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译：本发明涉及一种用于生产在由核酸的生物体的多不饱和脂肪酸的方法被引入到生物体中，其用于与酰基辅酶A的多肽代码：溶血磷脂酰基转移酶活性。有利地，这些核酸序列可以在转基因有机体与其他核酸的脂肪酸或脂质的多肽的代码序列表示任选在一起。本发明还涉及含有包含上述核酸序列，核酸构建体和/或载体中的本发明和/或所述核酸构建体和转基因生物的核酸序列的本发明的核酸序列，含有本发明的核酸序列的核酸构建体，载体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。

5. 发明申请

WO2004076617A3 METHOD FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS 审中-公开
标题翻译：生产多种不饱和脂肪酸的方法
公开(公告)号：WO2004076617A3
公开(公告)日：2004-10-28
申请号：PCT/EP2004000771
申请日：2004-01-29
申请人： BASF PLANT SCIENCE GMBH , RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE , DOMERGUE FREDERIC , ZANK THORSTEN
发明人： RENZ ANDREAS , HEINZ ERNST , ABBADI AMINE , DOMERGUE FREDERIC , ZANK THORSTEN
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing nucleic acids coding for polypeptides with acyl-CoA:lysophospholipid-acyl transferase activity into the organism. Advantageously, said nucleic acid sequences can optionally be expressed in the transgenic organism along with additional nucleic acid sequences coding for polypeptides of fatty acid metabolism or lipid metabolism biosynthesis. The invention also relates to the nucleic acid sequences, nucleic acid structures containing said nucleic acid sequences, vectors containing the inventive nucleic acid sequences, and/or the nucleic acid structures and transgenic organisms containing said nucleic acid sequences, nucleic acid structures, and/or vectors. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and the use thereof.
摘要翻译：本发明涉及一种用于生产在由核酸的生物体的多不饱和脂肪酸的方法被引入到生物体中，其用于与酰基辅酶A的多肽代码：溶血磷脂酰基转移酶酶活性的编码。有利地，这些核酸序列可以任选地与编码脂肪酸或脂质代谢的生物合成的多肽的其他核酸序列一起在转基因生物体中表达。本发明还涉及核酸序列，含有根据本发明的核酸序列的核酸构建体，包含核酸序列的载体和/或含有上述核酸序列的核酸构建体和转基因生物，核酸构建体和/或载体。本发明的另一部分涉及通过erfindungsgemäbetaen方法制备的油，脂质和/或脂肪酸及其用途。

6. 发明申请

WO2004090123A2 Delta-4 DESATURASES FROM EUGLENA GRACILIS, EXPRESSING PLANTS, AND OILS CONTAINING PUFA 审中-公开
标题翻译：来自小眼虫Δ-4去饱和酶，表达植物和多不饱和脂肪酸含油
公开(公告)号：WO2004090123A2
公开(公告)日：2004-10-21
申请号：PCT/EP2004003628
申请日：2004-04-06
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , MEYER ASTRID , HEINZ ERNST , ZAEHRINGER ULRICH
发明人： CIRPUS PETRA , BAUER JOERG , MEYER ASTRID , HEINZ ERNST , ZAEHRINGER ULRICH
IPC分类号： C12N9/02 , C12N15/82 , C12N9/00
CPC分类号： C12N9/0071 , C12N15/8247
摘要： The invention relates to an improved method for the specific production of unsaturated omega-3 fatty acids and a method for producing triglycerides containing an increased number of unsaturated fatty acids, especially omega-3 fatty acids comprising more than three double bonds. Disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism containing an increased number of fatty acids, oils, or lipids comprising Delta-4 double bonds as a result of a Delta-4 desaturase being expressed from Euglena gracilis. The invention further relates to expression cassettes containing a nucleic acid sequence, a vector, and organisms containing at least one nucleic acid sequence or an expression cassette. Also disclosed are unsaturated fatty acids and triglycerides having an increased fatty acid content and the use thereof. Fatty acids and triglycerides are used in a plurality of application in the food industry, in animal nutrition, cosmetics, and pharmaceuticals and are suitable for many different applications depending on whether free saturated or unsaturated fatty acids or triglycerides having an increased saturated or unsaturated fatty acid content are used.
摘要翻译：本发明涉及一种用于不饱和ω-3脂肪酸的具体赫斯位置和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的改进方法中，具有超过三个双键尤其ω-3脂肪酸。本发明涉及生产转基因生物，优选的转基因植物或转基因微生物，其具有的脂肪酸，油或脂类具有由于来自小眼虫的Δ-4去饱和酶的表达Δ-4个双键的含量增加。 urthermore，本发明涉及含有核酸序列，载体的表达盒和生物体含有至少一种核酸序列或表达盒。本发明还涉及不饱和脂肪酸和与Trigiyceride的不饱和脂肪酸含量增加及其用途。脂肪酸和Trigiyceride有多种食品工业中的应用，动物营养，化妆品和药品。取决于它们是否是游离饱和或不饱和脂肪酸或甘油三酯的饱和或不饱和脂肪酸的含量增加，它们适用于不同的应用。

7. 发明申请

WO2005083053A3 METHOD FOR PRODUCING UNSATURATED OMEGA-3 FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和ω-3脂肪酸的转基因生物
公开(公告)号：WO2005083053A3
公开(公告)日：2005-11-10
申请号：PCT/EP2005001865
申请日：2005-02-23
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , ZANK THORSTEN , HEINZ ERNST
发明人： CIRPUS PETRA , BAUER JOERG , ZANK THORSTEN , HEINZ ERNST
IPC分类号： C12N9/02 , C12N15/82 , C12P7/64 , C12N5/10 , C12N15/53
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The invention relates to a method for producing unsaturated O3 fatty acids, and to a method for producing triglycerides having an increased unsaturated fatty acid content, especially O3 fatty acids with more than three double bonds. The invention also relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic micro-organism, with an increased content of unsaturated O3 double bonds on the basis of the expression of a O3 desaturase of fungi of the pythiacae family, such as the species phytophtora, for example the species and type phytophtora infestans. The invention further relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing at least one inventive nucleic acid sequence, at least one vector containing the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms containing the cited nucleic acid sequences, nucleic acid constructs and/or vectors. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides having an increased unsaturated fatty acid content, and to the use of the same.
摘要翻译：本发明涉及一种用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的制造方法，具有多于三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于ω-3去饱和酶的来自家庭Pythiaceae的真菌表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加如属疫，例如属和种致病疫霉。本发明还涉及包含本发明的至少一种核酸序列，含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物的至少一种载体中的核酸序列，核酸构建体，载体，和生物体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

8. 发明申请

WO2005012316A3 METHOD FOR THE PRODUCTION OF MULTIPLY-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和脂肪酸的转基因多
公开(公告)号：WO2005012316A3
公开(公告)日：2005-10-06
申请号：PCT/EP2004007957
申请日：2004-07-16
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： A23D9/00 , C11B1/02 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64 , A01K67/027
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Delta-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Delta-6 desaturase, a Delta-5 desaturase, Delta-4 desaturase and/or Delta-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated omega-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Delta-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of omega-3 fatty acids, oils or lipids with omega-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with omega-3 desaturases, for example a omega-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a omega-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于通过核酸的生物体制备的多个unge饱和脂肪酸的方法被引入到生物体中，这对于多肽与Δ-5-延伸酶活性的代码。有利地，这些核酸序列可以在生物体中，任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。特别有利的是编码Δ-4-Desaturaseund /或Δ-6去饱和酶，Δ-5去饱和酶的Δ-6延长酶的活性的核酸序列。有利的是，这些去饱和酶和延伸酶海链藻，裸藻或Ostreococcus。此外，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。此外，在一个优选的实施方案中，本发明涉及用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的过程中，具有超过三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于延伸酶的表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加，并在本发明中连接有利的过程中去饱和酶使用与ω-3去饱和酶，例如，从家庭Pythiaceae的真菌，如属疫ω-3去饱和酶，例如属和种致病疫霉或从藻类ω-3去饱和酶诸如家庭绿枝藻纲例如的属Ostreococcus具体的属和种Ostreococcus桃源或硅藻如海链藻属特别是属和种海链pseudonana。本发明还涉及含有本发明核酸序列的核酸序列，核酸构建体，载体和有机体，将含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物体的载体。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

9. 发明申请

WO02057465A3 METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS, NOVEL BIOSYNTHESIS GENES AND NOVEL PLANT EXPRESSION CONSTRUCTS 审中-公开
标题翻译：用于生产多不饱和脂肪酸，合成新的和新工厂的表达构建
公开(公告)号：WO02057465A3
公开(公告)日：2003-06-12
申请号：PCT/EP0200462
申请日：2002-01-18
申请人： BASF PLANT SCIENCE GMBH , LERCHL JENS , RENZ ANDREAS , HEINZ ERNST , DOMERGUE FREDERIC , ZAEHRINGER ULRICH
发明人： LERCHL JENS , RENZ ANDREAS , HEINZ ERNST , DOMERGUE FREDERIC , ZAEHRINGER ULRICH
IPC分类号： A01H5/00 , A01K67/027 , A23D9/007 , A23L1/30 , A61K8/30 , A61K8/34 , A61K8/37 , A61K31/201 , A61K31/202 , A61K31/231 , A61K31/232 , A61K31/7088 , A61K36/00 , A61K39/395 , A61K45/00 , A61P3/06 , C07K16/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/02 , C12N15/09 , C12N15/53 , C12N15/82 , C12P7/64 , C12Q1/02 , C12Q1/26 , G01N33/15 , G01N33/50 , C11B1/00 , C12N15/11 , C12P7/62 , C12Q1/68 , G01N33/573
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a method for producing unsaturated fatty acids comprising at least two double bonds, and a method for producing triglycerides having an increased content of polyunsaturated fatty acids comprising at least two double bonds. The invention also relates to the advantageous use of the nucleic acid sequences of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11 or SEQ ID NO: 18 in the inventive methods, and for producing a transgenic organism, preferably a transgenic plant or a transgenic micro-organism having an increased content of fatty acids, oils or lipids containing unsaturated C18, C20, or C22 fatty acids. The invention further relates to novel desaturases comprising the sequences of SEQ ID NO. 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11, or the homologues, derivatives or analogues thereof, and gene constructs comprising said genes or the homologues, derivatives or analogues thereof, in addition to the use of the same, either alone or combined with biosynthesis genes of polyunsaturated fatty acids, as advantageously represented in SEQ ID NO: 7 and SEQ ID NO: 9. Furthermore, the invention relates to isolated nucleic acid sequences, expression cassettes containing said nucleic acid sequences, vectors, and transgenic organisms containing at least one nucleic acid sequence or an expression cassette. The invention also relates to unsaturated fatty acids comprising at least two double bonds and triglycerides having an increased content of unsaturated fatty acids comprising at least two double bonds, and the use of the same. Finally, the invention relates to multi-expression cassettes for seed-specific expression, in addition to vectors or organisms comprising a desaturase gene, either alone or combined with other desaturases having the sequence of SEQ ID NO: 7 and/or elongase genes having the sequence of ID NO: 9 or the homologues, derivatives or analogues of the same, expressed by means of the above-mentioned expression cassettes.
摘要翻译：本发明涉及一种用于制备具有至少两个双键和/或用于生产甘油三酯的具有多不饱和脂肪酸与多不饱和脂肪酸具有至少两个双键的至少höhtem含量增加的内容的方法的不饱和脂肪酸的方法。本发明还涉及有利地使用的核酸序列的SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5，SEQ ID NO：9，SEQ ID NO：11或SEQ ID NO：18中的过程和用于生产的转基因生物体，优选转基因植物或具有脂肪酸，油或脂类具有不饱和C18，C20，C22或脂肪酸含量增加的转基因微生物。本发明还涉及新的去饱和酶与序列SEQ ID号 1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：11或它的同系物，衍生物或它们的类似物，和包含所述基因的基因构建体或他们的同系物，衍生物或类似物，并单独或与生物合成基因组合使用它们polyungesätigter 脂肪酸，例如有利地在SEQ ID NO：9：7和SEQ ID NO。本发明还涉及分离的核酸序列; 表达盒包含Nukleinesäuresequenzen。载体，以及含有至少一种核酸序列或表达盒的转基因生物。本发明还涉及一种具有至少两个双键和具有具有至少两个双键和其用途的不饱和脂肪酸含量增加的甘油三酯不饱和脂肪酸。本发明还涉及用于种子特异性表达，其包括单独或与具有SEQ ID NO：1的序列的其它去饱和酶组合的去饱和酶基因的多表达盒和载体或生物体：7和/或延长酶基因与去SEQ ID NO：9或它的同系物，衍生物或类似物包括使用vesagter的表达盒。

10. 发明申请

WO02057464A3 METHOD FOR THE EXPRESSION OF BIOSYNTHETIC GENES IN PLANT SEEDS USING MULTIPLE EXPRESSION CONSTRUCTS 审中-公开
标题翻译：为了表达蔬菜种子的生物合成基因的方法是在使用多个表达构建
公开(公告)号：WO02057464A3
公开(公告)日：2003-02-27
申请号：PCT/EP0200461
申请日：2002-01-18
申请人： BASF PLANT SCIENCE GMBH , LERCHL JENS , DUWENIG ELKE , BISCHOFF FRIEDRICH , HEINZ ERNST , DREXLER HJOERDIS , SCHEFFLER JODI
发明人： LERCHL JENS , DUWENIG ELKE , BISCHOFF FRIEDRICH , HEINZ ERNST , DREXLER HJOERDIS , SCHEFFLER JODI
IPC分类号： C12N9/02 , C12N9/10 , C12N15/29 , C12N15/53 , C12N15/54 , C12N15/82 , A01H5/00 , C12N15/81 , C12P7/64
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247
摘要： The invention relates to expression cassettes, combinations thereof and vectors containing said expression cassettes, containing plant promoters with an expression specificity for plant seeds, in particular linseed and the use of said expression cassettes or vectors for the recombinant expression of heterologous genes in plants. The invention further relates to transgenic plants, transformed by means of said expression cassettes, or vectors, cultures, parts or transgenic propagations derived therefrom and the use of the above as foodstuff, animal feedstuff, seedstuff, pharmaceuticals, fine chemicals or industrial raw material.
摘要翻译：本发明涉及一种表达盒，其组合以及含有包含植物启动子用表达特异性植物种子，特别是亚麻子和异源基因在植物中的重组表达使用这些表达盒或载体的表达盒的载体。这些表达盒或转化的培养物从，部件或转基因繁殖材料衍生的转基因植物的载体，并且其用作食品，饲料或种子，医药品，精细化学品或工业原料本发明进一步涉及。

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