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1. 发明申请

WO2005012316A3 METHOD FOR THE PRODUCTION OF MULTIPLY-UNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和脂肪酸的转基因多
公开(公告)号：WO2005012316A3
公开(公告)日：2005-10-06
申请号：PCT/EP2004007957
申请日：2004-07-16
申请人： BASF PLANT SCIENCE GMBH , ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
发明人： ZANK THORSTEN , BAUER JOERG , CIRPUS PETRA , ABBADI AMINE , HEINZ ERNST , QIU XIAO , VRINTEN PATRICIA , SPERLING PETRA , DOMERGUE FREDERIC , MEYER ASTRID , KIRSCH JELENA
IPC分类号： A23D9/00 , C11B1/02 , C12N9/02 , C12N9/10 , C12N15/82 , C12P7/64 , A01K67/027
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Delta-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Delta-6 desaturase, a Delta-5 desaturase, Delta-4 desaturase and/or Delta-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated omega-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Delta-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of omega-3 fatty acids, oils or lipids with omega-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with omega-3 desaturases, for example a omega-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a omega-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于通过核酸的生物体制备的多个unge饱和脂肪酸的方法被引入到生物体中，这对于多肽与Δ-5-延伸酶活性的代码。有利地，这些核酸序列可以在生物体中，任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。特别有利的是编码Δ-4-Desaturaseund /或Δ-6去饱和酶，Δ-5去饱和酶的Δ-6延长酶的活性的核酸序列。有利的是，这些去饱和酶和延伸酶海链藻，裸藻或Ostreococcus。此外，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。此外，在一个优选的实施方案中，本发明涉及用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的过程中，具有超过三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于延伸酶的表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加，并在本发明中连接有利的过程中去饱和酶使用与ω-3去饱和酶，例如，从家庭Pythiaceae的真菌，如属疫ω-3去饱和酶，例如属和种致病疫霉或从藻类ω-3去饱和酶诸如家庭绿枝藻纲例如的属Ostreococcus具体的属和种Ostreococcus桃源或硅藻如海链藻属特别是属和种海链pseudonana。本发明还涉及含有本发明核酸序列的核酸序列，核酸构建体，载体和有机体，将含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物体的载体。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

2. 发明申请

WO2006069936A3 METHOD FOR PRODUCING POLYUNSATURATED LONG-CHAIN FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：过程在转基因生物生产多种不饱和长链脂肪酸
公开(公告)号：WO2006069936A3
公开(公告)日：2007-03-22
申请号：PCT/EP2005056957
申请日：2005-12-20
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , HEINZ ERNST , ABBADI AMINE , KIRSCH JELENA
发明人： CIRPUS PETRA , BAUER JOERG , HEINZ ERNST , ABBADI AMINE , KIRSCH JELENA
IPC分类号： C12N9/10 , A01K67/027 , C12N5/10 , C12N15/54
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译：本发明涉及一种用于生产在由核酸的生物体的多不饱和脂肪酸的方法被引入到生物体中，其用于与酰基辅酶A的多肽代码：溶血磷脂酰基转移酶活性。有利地，这些核酸序列可以在转基因有机体与其他核酸的脂肪酸或脂质的多肽的代码序列表示任选在一起。本发明还涉及含有包含上述核酸序列，核酸构建体和/或载体中的本发明和/或所述核酸构建体和转基因生物的核酸序列的本发明的核酸序列，含有本发明的核酸序列的核酸构建体，载体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。

3. 发明申请

WO2005083053A3 METHOD FOR PRODUCING UNSATURATED OMEGA-3 FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：用于生产不饱和ω-3脂肪酸的转基因生物
公开(公告)号：WO2005083053A3
公开(公告)日：2005-11-10
申请号：PCT/EP2005001865
申请日：2005-02-23
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , ZANK THORSTEN , HEINZ ERNST
发明人： CIRPUS PETRA , BAUER JOERG , ZANK THORSTEN , HEINZ ERNST
IPC分类号： C12N9/02 , C12N15/82 , C12P7/64 , C12N5/10 , C12N15/53
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The invention relates to a method for producing unsaturated O3 fatty acids, and to a method for producing triglycerides having an increased unsaturated fatty acid content, especially O3 fatty acids with more than three double bonds. The invention also relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic micro-organism, with an increased content of unsaturated O3 double bonds on the basis of the expression of a O3 desaturase of fungi of the pythiacae family, such as the species phytophtora, for example the species and type phytophtora infestans. The invention further relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing at least one inventive nucleic acid sequence, at least one vector containing the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms containing the cited nucleic acid sequences, nucleic acid constructs and/or vectors. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides having an increased unsaturated fatty acid content, and to the use of the same.
摘要翻译：本发明涉及一种用于生产不饱和ω-3脂肪酸和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的制造方法，具有多于三个双键尤其ω-3脂肪酸。本发明涉及产生转基因生物，优选的转基因植物或转基因微生物，其具有具有由于ω-3去饱和酶的来自家庭Pythiaceae的真菌表达ω-3双键的不饱和的ω-3脂肪酸，油或脂质的含量增加如属疫，例如属和种致病疫霉。本发明还涉及包含本发明的至少一种核酸序列，含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物的至少一种载体中的核酸序列，核酸构建体，载体，和生物体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

4. 发明申请

WO2004090123A2 Delta-4 DESATURASES FROM EUGLENA GRACILIS, EXPRESSING PLANTS, AND OILS CONTAINING PUFA 审中-公开
标题翻译：来自小眼虫Δ-4去饱和酶，表达植物和多不饱和脂肪酸含油
公开(公告)号：WO2004090123A2
公开(公告)日：2004-10-21
申请号：PCT/EP2004003628
申请日：2004-04-06
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , MEYER ASTRID , HEINZ ERNST , ZAEHRINGER ULRICH
发明人： CIRPUS PETRA , BAUER JOERG , MEYER ASTRID , HEINZ ERNST , ZAEHRINGER ULRICH
IPC分类号： C12N9/02 , C12N15/82 , C12N9/00
CPC分类号： C12N9/0071 , C12N15/8247
摘要： The invention relates to an improved method for the specific production of unsaturated omega-3 fatty acids and a method for producing triglycerides containing an increased number of unsaturated fatty acids, especially omega-3 fatty acids comprising more than three double bonds. Disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism containing an increased number of fatty acids, oils, or lipids comprising Delta-4 double bonds as a result of a Delta-4 desaturase being expressed from Euglena gracilis. The invention further relates to expression cassettes containing a nucleic acid sequence, a vector, and organisms containing at least one nucleic acid sequence or an expression cassette. Also disclosed are unsaturated fatty acids and triglycerides having an increased fatty acid content and the use thereof. Fatty acids and triglycerides are used in a plurality of application in the food industry, in animal nutrition, cosmetics, and pharmaceuticals and are suitable for many different applications depending on whether free saturated or unsaturated fatty acids or triglycerides having an increased saturated or unsaturated fatty acid content are used.
摘要翻译：本发明涉及一种用于不饱和ω-3脂肪酸的具体赫斯位置和用于生产具有的不饱和脂肪酸含量增加的甘油三酯的方法的改进方法中，具有超过三个双键尤其ω-3脂肪酸。本发明涉及生产转基因生物，优选的转基因植物或转基因微生物，其具有的脂肪酸，油或脂类具有由于来自小眼虫的Δ-4去饱和酶的表达Δ-4个双键的含量增加。 urthermore，本发明涉及含有核酸序列，载体的表达盒和生物体含有至少一种核酸序列或表达盒。本发明还涉及不饱和脂肪酸和与Trigiyceride的不饱和脂肪酸含量增加及其用途。脂肪酸和Trigiyceride有多种食品工业中的应用，动物营养，化妆品和药品。取决于它们是否是游离饱和或不饱和脂肪酸或甘油三酯的饱和或不饱和脂肪酸的含量增加，它们适用于不同的应用。

5. 发明申请

WO2005083093A3 METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC PLANTS 审中-公开
标题翻译： PROCESS转基因植物中多产多不饱和脂肪酸
公开(公告)号：WO2005083093A3
公开(公告)日：2006-02-16
申请号：PCT/EP2005001863
申请日：2005-02-23
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI
发明人： CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI
IPC分类号： C11B1/02 , C12N9/02 , C12N9/10 , A01K67/027
CPC分类号： C12N15/82 , C12N15/8247
摘要： The invention relates to a method for producing polyunsaturated fatty acids in seeds of transgenic plants. According to said method, nucleic acids, coding for polypeptides with a ?-3-desaturase, ?-12-desaturase, ?-6-desaturase, ?-6-elongase, ?-5-desaturase, ?-5-elongase and/or ?-4-desaturase activity, preferably for polypeptides with a ?-6-desaturase, ?-6-elongase and ?-5-desaturase activity, are introduced into the organism. The nucleic acid sequences are represented by SEQ ID NO: 11, SEQ ID NO: 27, SEQ ID NO: 193, SEQ ID NO: 197, SEQ ID NO: 199 and SEQ ID NO: 201. Advantageously, said nucleic acid sequences can be expressed in the organism optionally together with other nucleic acid sequences that code for polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Nucleic acid sequences coding for a ?-6-desaturase, ?-5-desaturase, ?-4-desaturase, ?-1 2-desaturase and/or ?-6-elongase activity are especially advantageous. Advantageously, said desaturases and elongases originate from thalassiosira, euglena or ostreococcus. The invention also relates to a method for producing oils and/or triacylglycerides with an increased content of long-chain polyunsaturated fatty acids. In a preferred embodiment, the invention also relates to a method for producing arachidonic acid, eicosapentaenic acid or docosahexaenic acid, and to a method for producing triglycerides with an increased content of unsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, in transgenic plants, preferably in seeds of the transgenic plants. The invention further relates to the production of a transgenic plant with an increased content of polyunsaturated fatty acids, especially arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, based on the expression of the elongases and desaturases used in the inventive method. The invention also relates to recombinant nucleic acids molecules containing, together or individually, nucleic acid sequences coding for the polypeptides with a ?-6-desaturase, ?-6-elongase, ?-5-desaturase and ?-5-elongase activity, and transgenic plants containing said recombinant nucleic acid molecules. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use thereof. Furthermore, the invention relates to unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids, and to the use of the same.
摘要翻译：本发明涉及一种用于生产由核酸的转基因植物的种子中的多不饱和脂肪酸的方法被引入到生物体中，其与β-3去饱和酶，？-12-去饱和酶，？-6去饱和酶编码多肽，？-6-延伸,? -5去饱和酶，？-5-延伸酶和/或Δ4-去饱和酶优选的多肽具有？-6去饱和酶，？-6延伸酶和？-5- 脱氢酶活性。的核酸序列示于SEQ ID NO：11，SEQ ID NO：27，SEQ ID NO：193，SEQ ID NO：197，SEQ ID NO：199和SEQ ID NO：所示第二百○一序列有利地，这些核酸序列可以在生物体中，任选地与编码所述脂肪酸或脂质的生物合成的多肽的另外的核酸序列表达。特别有利的是编码一个？-6去饱和酶中，α-1 2去饱和酶-6-延伸-5去饱和酶，？4去饱和酶，？和/或核酸序列？有利的是，这些去饱和酶和延伸酶海链藻，裸藻或Ostreococcus。此外，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。本发明中的优选实施例还涉及一种用于生产花生四烯酸，二十碳五烯酸或二十二碳六酸和用于生产甘油三酯的与不饱和脂肪酸含量增加的处理，特别是花生四烯酸，二十碳五烯酸和/或二十碳六烯酸的，有利的是在转基因植物中的转基因的种子植物。本发明涉及生产具有多不饱和脂肪酸，特别是花生四烯酸，二十碳五烯酸和/或二十碳六烯酸，含量增加的转基因植物由于延伸酶的表达和在本发明的去饱和酶处理中使用。本发明还涉及含有核酸序列，其的重组核酸分子编码的多肽具有？-6去饱和酶，？-6-延伸,? -5去饱和酶和β-5-延伸酶，联合或单独，以及含有上述的重组核酸分子的转基因植物。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

6. 发明申请

WO2006100241A3 METHOD FOR PRODUCING POLYUNSATURATED C20 AND C22FATTY ACIDS WITH AT LEAST FOUR DOUBLE BONDS IN TRANSGENIC PLANTS 审中-公开
标题翻译：用于生产多不饱和C20和C22脂肪酸与至少四种转基因植物中的双键
公开(公告)号：WO2006100241A3
公开(公告)日：2006-12-07
申请号：PCT/EP2006060913
申请日：2006-03-21
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG
发明人： CIRPUS PETRA , BAUER JOERG
IPC分类号： C12N15/82 , C12P7/64
CPC分类号： C12P7/6472 , C12N9/0083 , C12N9/1029 , C12N15/8247 , C12P7/6427
摘要： The invention relates to a method for producing polyunsaturated fatty acids in transgenic plants, whereby nucleic acids coding for polypeptides with ?-6-desaturase, ?-6-elongase, ?-5-desaturase, ?-5-elongase, ?-4-desaturase, ?-12-desaturase and/or ?-3-desaturase activity are introduced into the plants. Advantageously, said desaturases and elongases come from phytophtora sojae. The invention also relates to nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid constructs, and transgenic plants containing the cited nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to fatty acid compositions produced according to the inventive method, and to the use thereof.
摘要翻译：本发明涉及一种用于生产转基因植物中的多不饱和脂肪酸的方法，该植物被导入核酸编码的多肽具有？-6去饱和酶，？-6-延长酶，？-5去饱和酶， -4去饱和酶编码-3-去饱和酶活性？-5-延伸酶，？，？-12去饱和酶和/或?. 有利的是，这些去饱和酶和延伸大豆疫霉菌来了。本发明还涉及包含本发明的核酸序列的核酸序列，核酸构建体，载体和有机体，将含有核酸序列和/或核酸构建体，和转基因植物载体含有上述核酸序列，核酸构建体和/或载体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的脂肪酸组合物。

7. 发明申请

WO2006008099A3 METHOD FOR INCREASING THE CONTENT OF POLYUNSATURATED LONG-CHAINED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：方法用于增加转基因生物多不饱和长链脂肪酸的含量
公开(公告)号：WO2006008099A3
公开(公告)日：2007-03-15
申请号：PCT/EP2005007754
申请日：2005-07-15
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , QIU XIAO , VRINTEN PATRICIA
发明人： CIRPUS PETRA , BAUER JOERG , QIU XIAO , VRINTEN PATRICIA
IPC分类号： C12N15/82 , A01H5/00 , C12N9/04 , C12N9/20 , C12N9/88 , C12N15/53 , C12N15/55 , C12N15/60
CPC分类号： C12N9/0006 , C12N9/20 , C12N9/88 , C12N15/8247
摘要： The invention relates to a method for increasing the content of polyunsaturated long-chained fatty acids in an organism, by introducing nucleic acid coding for polypeptides and/or proteins having phopholipase, ketoacyl-CoA-reductase and/or dehydratase activity into the organisms. Advantageously, said enzymes stem from ostreococcus or thraustochytrium. The invention also relates to nucleic acid sequences, nucleic acid structures, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid structures, and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid structures and/or vectors. Advantageously, said above-mentioned nucleic acid frequencies, nucleic acid structures and vectors can optionally be expressed in the organism along with additional nucleic acid sequences coding for the polypeptides and/or proteins of fatty acids or lipid metabolism biosynthesis. Another advantage of the nucleic acid sequences of the fatty acids or lipid metabolism is that they are coded for ?-9 elongase, ?-8-desaturase, a ?-5-desaturase, ?-4-desaturase, ?-12-desaturase, ?-5-elongase and/or ?-6-elongase activity. Said desaturases and elongases stem from organisms such as thalassiosira, euglena, isochrysis, physcomitrella, thraustochytrium, borago, phytophthora, crypthecodinium, oncorhynchus, primula, xenopus, ciona, arabidopsis, mortierella, caenorhabditis, phaeodactylum, ceratodon or ostreococcus. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and to the use thereof, in addition to the inventive unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and to the use thereof.
摘要翻译：本发明涉及一种用于被引入到生物体中，在增加的核酸的生物体的多不饱和长链脂肪酸含量，其编码具有Phopholipase-，脂酰CoA还原酶和/或脱水酶活性的多肽或蛋白质编码。有利的是，这些酶是从Ostreococcus或破囊壶菌。本发明还涉及包含本发明核酸序列的核酸序列，核酸构建体，载体，和生物体，包含含有上述Nukleinsäuersequenzen，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物体的载体。有利地，这些上述核酸序列，Nukleainsäurekonstrukte，载体可在生物体与编码多肽或所述脂肪酸或脂质的生物合成的蛋白质的进一步的核酸序列表达，任选一起。所述脂肪酸或脂质的特别有利的核酸序列所必需的一个？-9延长酶？-8-去饱和酶，？-5去饱和酶，？4去饱和酶，？-12-去饱和酶，？-5 编码-Elongase-和/或ω-6延伸酶活性。有利地，这些去饱和酶和从生物体如海链，眼虫，等鞭金藻，小立碗，破囊壶菌属，琉璃苣，疫霉，隐甲藻属，大马哈鱼，报春花，非洲爪蟾，玻璃海鞘，拟南芥，被孢霉属，秀丽隐杆线虫，褐指藻属，角齿或Ostreococcus衍生延伸酶。本发明的另一部分涉及通过本发明和它们的使用过程中产生的油，脂质和/或脂肪酸。本发明还涉及到的不饱和脂肪酸和甘油三酯具有不饱和脂肪酸的含量增加及其用途。

8. 发明申请

WO2007017419A3 METHOD FOR PRODUCING ARACHIDONIC ACID AND/OR EICOSAPENTAENOIC ACID IN USEFUL TRANSGENIC PLANTS 审中-公开
标题翻译：在转基因植物中生产阿拉酸和/或艾考酸的方法
公开(公告)号：WO2007017419A3
公开(公告)日：2007-05-10
申请号：PCT/EP2006064922
申请日：2006-08-01
申请人： BASF PLANT SCIENCE GMBH , CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI , TRUKSA MARTIN
发明人： CIRPUS PETRA , BAUER JOERG , QIU XIAO , WU GUOHAI , DATLA NAGAMANI , TRUKSA MARTIN
IPC分类号： C12N15/82 , C07K14/415 , C11B1/02 , C12N9/02
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A61K8/361 , A61Q19/00 , C12N9/0083 , C12N9/1029
摘要： The invention relates to a method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants, according to which nucleic acids, which encode polypeptides with ?-6-desaturase activity, ?-6-elongase activity or ?-5-desaturase activity, are introduced into the plants. As an added advantage, a gene that encodes a ?-3-desaturase is expressed in the useful plants. In an additional embodiment of the method, additional nucleic acid sequences, which encode polypeptides involved in the biosynthesis of the fatty acid or lipid metabolism, can be expressed in the plants. Particularly advantageous nucleic acid sequences are those that encode ?-8-desaturase activity, ?-12-desaturase activity, ?-15-desaturase activity, ?-4-desaturase activity, ?-9-elongase activity and/or ?-5-elongase activity. The invention also relates to the use of the oils, lipids and/or fatty acids that have been produced by said method in animal feed or foodstuffs, cosmetics or pharmaceutical products.
摘要翻译：本发明涉及用于生产花生四烯酸和/或通过核酸的转基因作物植物的二十碳五烯酸的方法被引入到编码的多肽具有其中植物？-6去饱和酶，？-6-延伸酶或？-5去饱和酶活性，有利的是，编码α-3-去饱和酶的基因还在农作物中表达。在该方法的另一个有利的实施方案中，可以在植物中表达编码脂肪酸或脂质代谢的生物合成的多肽的其他核酸序列。这是编码的8去饱和酶特别有利的核酸序列 - “ - ？-9-延伸12去饱和酶，-15去饱和酶，-4-去饱和酶，和/或???? - 编码5-延伸酶活性。本发明的另一部分涉及使用的在饲料或食品，化妆品或药品中的本发明方法的油，脂质和/或脂肪酸的制造。

9. 发明申请

WO2004087902A2 NOVEL PLANT ACYLTRANSFERASES SPECIFIC FOR LONG-CHAINED, MULTIPLY UNSATURATED FATTY ACIDS 审中-公开
标题翻译：新工厂酰基转移酶特异性多不饱和脂肪酸的长链
公开(公告)号：WO2004087902A2
公开(公告)日：2004-10-14
申请号：PCT/EP2004003224
申请日：2004-03-26
申请人： UNIV BRISTOL , RENZ ANDREAS , BAUER JOERG , FRENTZEN MARGIT , SOEZER NURSEN , KEITH STOBART , FRASER THOMAS , LAZARUS COLIN M , QI BAOXIU , ABBADI AMINE , HEINZ ERNST
发明人： RENZ ANDREAS , BAUER JOERG , FRENTZEN MARGIT , SOEZER NURSEN , KEITH STOBART , FRASER THOMAS , LAZARUS COLIN M , QI BAOXIU , ABBADI AMINE , HEINZ ERNST
IPC分类号： C12N9/10 , C12N15/82 , C12P7/64 , A01H5/00
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The invention relates to a method for the production of long-chained, multiply unsaturated fatty acids in an organism, wherein nucleic acids coding for polypeptide with acyltransferase activity are introduced into the organism. Said nucleic acid sequences can be advantageously expressed in the organism, optionally together with other nucleic acid sequences coding for polypeptides of the biosynthesis of the fatty acid or lipid metabolism. The invention also relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chained, multiply unsaturated fatty acids. The invention further relates to the nucleic acid sequences, nucleic acids constructs vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or nucleic acid constructs and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid constructs and/or vectors. The invention additionally relates to oils, lipids and/or fatty acids produced according to the inventive method and to the utilization thereof.
摘要翻译：本发明涉及一种用于通过核酸的生物体制备的长链多不饱和脂肪酸被引入到生物体中，这为具有酰基转移酶活性的多肽的代码的处理。有利地，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成的多肽的另外的核酸序列可以在生物体中表达一起任选。更远地，本发明涉及用于生产油和/或三酰基甘油酯与长链多不饱和脂肪酸的升高的内容的处理。本发明还涉及含有本发明核酸序列的核酸序列，核酸构建体，载体和有机体，将含有包含上述核酸序列，核酸构建体和/或载体的核酸序列和/或核酸构建体，和转基因生物体的载体。本发明的另一部分涉及根据本发明和它们的使用由该方法产生的油，脂质和/或脂肪酸。

10. 发明申请

WO2010066703A3 DESATURASES AND PROCESS FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：在转基因生物中生产聚氨酯脂肪酸的苦味和过程
公开(公告)号：WO2010066703A3
公开(公告)日：2010-09-23
申请号：PCT/EP2009066569
申请日：2009-12-08
申请人： BASF PLANT SCIENCE GMBH , SENGER TORALF , BAUER JOERG , NAPIER JOHNATHAN A
发明人： SENGER TORALF , BAUER JOERG , NAPIER JOHNATHAN A
IPC分类号： C12N15/82 , A01H5/00 , C12N9/02 , C12N15/53 , C12P7/64
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , C12P7/6481 , C12Y114/19001
摘要： The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recom bi nant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.
摘要翻译：本发明涉及来自嗜球菌（Cocchioiobolus heterostrophus）C5，蓝藻（Cyanothece sp。）的多核苷酸 CCY0110，Acycina霉菌和Hyaloperonospora parasitica，其代码为去饱和酶，可用于多不饱和脂肪酸的重复生产。本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。本发明还涉及针对根据本发明的多肽的抗体。最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。

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