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1. 发明申请

WO03089665A8 METHODS FOR MEASURING PROTEIN KINASE AND PHOSPHATASE ACTIVITY 审中-公开
标题翻译：测定蛋白激酶和磷酸酶活性的方法
公开(公告)号：WO03089665A8
公开(公告)日：2004-11-18
申请号：PCT/GB0301683
申请日：2003-04-17
申请人： AMERSHAM BIOSCIENCES UK LTD , WHATELEY JOHN GERARD
发明人： WHATELEY JOHN GERARD
IPC分类号： G01N33/50 , C12N9/10 , C12N9/16 , C12Q1/42 , C12Q1/48 , G01N21/78 , G01N33/15
CPC分类号： C12Q1/485 , C12Q1/42
摘要： The invention provides fluorescence-based assays for determining the phosphorylating or the dephosphorylating activity of an enzyme acting on a substrate molecule. The substrate comprises at least one moiety that is capable of being phosphorylated or dephosphorylated by an enzyme to yield a product, the substrate being labelled with a fluorescent dye, and which is capable of exhibiting change in fluorescence intensity and fluorescence lifetime upon phosphorylation of the dye-labelled substrate or upon dephosphorylation of the dye-labelled phosphorylated substrate. In preferred embodiments, the substrate is labelled with a fluorescent dye selected from the acridone and the quinacridone classes of dyes. Also provided is a method for screening for a test agent whose effect on the phosphorylating or dephosphorylating activity of an enzyme is to be determined.
摘要翻译：本发明提供用于测定作用于底物分子的酶的磷酸化或去磷酸化活性的基于荧光的测定。底物包含能够被酶磷酸化或去磷酸化以产生产物的至少一个部分，该底物用荧光染料标记，并且能够在染料磷酸化时显示出荧光强度和荧光寿命的变化标记的底物或染料标记的磷酸化底物的去磷酸化。在优选的实施方案中，用选自吖啶酮和喹吖啶酮类染料的荧光染料标记底物。还提供了筛选对酶的磷酸化或去磷酸化活性的影响的测试剂的方法。

2. 发明申请

WO2004009847A3 FUNCTIONAL SCREENING METHOD INVOLVING PROTEIN TRANSLOCATION/REDISTRIBUTION 审中-公开
标题翻译：涉及蛋白质转录/重新分配的功能筛选方法
公开(公告)号：WO2004009847A3
公开(公告)日：2004-04-15
申请号：PCT/GB0302983
申请日：2003-07-10
申请人： AMERSHAM BIOSCIENCES UK LTD , O'BEIRNE GERARD , THOMAS NICHOLAS
发明人： O'BEIRNE GERARD , THOMAS NICHOLAS
IPC分类号： C12N15/09 , C12Q1/02 , C12Q1/68 , G01N33/50 , G06F19/00 , C12N15/10 , G01N33/68
CPC分类号： G01N33/5035 , C12Q1/6876 , C12Q1/6897
摘要： The present invention provides high-throughput functional genomic methods for determininggene and protein function in a cellular context. Also provided are methods for identifying chemical modulators of gene and protein/enzyme activity. Assays are generated in concert with screening in an iterative process which expands the scope of biological coverage with each iteration and which uses image-based analysis to yield data at sub-cellular resolution.
摘要翻译：本发明提供用于在细胞上下文中确定基因和蛋白质功能的高通量功能基因组方法。还提供了用于鉴定基因和蛋白质/酶活性的化学调节剂的方法。测定是在迭代过程中与筛选一起产生的，每个迭代扩展生物覆盖范围，并使用基于图像的分析来产生亚细胞分辨率的数据。

3. 发明申请

WO03050518A3 SYSTEM AND METHOD FOR TIME CORRELATED MULTI-PHOTON COUNTING MEASUREMENTS 审中-公开
标题翻译：用于时间相关多光子计数测量的系统和方法
公开(公告)号：WO03050518A3
公开(公告)日：2003-07-17
申请号：PCT/GB0205543
申请日：2002-12-06
申请人： AMERSHAM BIOSCIENCES UK LTD , LABARBE RUDI , SEWELL ROGER , PUMPHERY SAM
发明人： LABARBE RUDI , SEWELL ROGER , PUMPHERY SAM
IPC分类号： G01N21/64
CPC分类号： G01N21/6408
摘要： The invention provides a method and a measurement system for characterisation of luminescence properties, the method comprises irradiating the luminescent material with a pulse of excitation light, providing a triggering signal correlated to the pulse of excitation light; detecting with a photodetector such as a photomultiplier tube (PMT) a plurality of photons emitted from the luminescent material as result of the pulse of excitation light, the photodetector providing an output signal upon the event of detection of a photon; determining for each detected photon a photon arrival time and providing an output suitable for inputting to an analysing module wherein an output comprises zero, one, or more photon arrival time for each excitation, receiving said outputs in an analysing module; and determining in the analysing module, characteristics properties of the luminescent material by performing a statistical analysis based on Bayesian inference.
摘要翻译：本发明提供一种用于表征发光特性的方法和测量系统，该方法包括用激发光脉冲照射发光材料，提供与激发光脉冲相关的触发信号; 用光电倍增管（PMT）等光电检测器检测由激发光脉冲引起的从发光材料发射的多个光子，光检测器在检测光子时提供输出信号; 为每个检测到的光子确定光子到达时间并提供适于输入到分析模块的输出，其中输出包括用于每个激励的零个，一个或多个光子到达时间，在分析模块中接收所述输出; 并通过执行基于贝叶斯推理的统计分析，在分析模块中确定发光材料的特性。

4. 发明申请

WO0157237A3 METHODS EMPLOYING INCREASED DYE-FLUORESCENCE UPON NITROREDUCTION 审中-公开
标题翻译：使用增加荧光染色剂的方法
公开(公告)号：WO0157237A3
公开(公告)日：2002-05-16
申请号：PCT/GB0100396
申请日：2001-02-01
申请人： AMERSHAM BIOSCIENCES UK LTD , THOMAS NICHOLAS , MICHAEL NIGEL PAUL , MILLAR VALERIE , DAVIES BETH , BRIGGS MARK SAMUEL JONATHAN
发明人： THOMAS NICHOLAS , MICHAEL NIGEL PAUL , MILLAR VALERIE , DAVIES BETH , BRIGGS MARK SAMUEL JONATHAN
IPC分类号： G01N33/533 , C09B23/00 , C09B23/08 , C09B67/22 , C09K11/07 , C12P17/12 , C12Q1/26 , G01N21/78 , G01N33/535 , G01N33/543 , G01N33/84 , G01N33/58 , C09B23/06 , G01N33/542
CPC分类号： C09B23/08 , C09B67/0033 , G01N33/84
摘要： Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterised by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.
摘要翻译：公开了增加包含至少一个NO 2基团的花青染料分子的荧光的方法，其特征在于通过硝基还原酶的作用将至少一种NO 2基团还原成NHOH或NH 2。包含至少一个NO 2基团的花青染料分子可用作组合物中硝基还原酶活性检测的底物，并且允许在酶报告系统中使用硝基还原酶用于检测分析物，结合反应或基因表达。

5. 发明申请

WO03014743A3 USE OF DENDRIMERS AND POLY-BRANCHED MOLECULES TO ENHANCE SIGNAL IN FLUORESCENT ASSAY SYSTEMS 审中-公开
标题翻译：使用树枝状分子和多分支分子来增强荧光分析系统中的信号
公开(公告)号：WO03014743A3
公开(公告)日：2003-06-19
申请号：PCT/GB0203517
申请日：2002-07-31
申请人： AMERSHAM BIOSCIENCES UK LTD , CUMMINS WILLIAM J , HAMILTON ALAN L , BRADLEY MARK , ELLARD JOHN , ZOLLITSCH THOMAS , BRIGGS MARK SAMUEL JONATHAN
发明人： CUMMINS WILLIAM J , HAMILTON ALAN L , BRADLEY MARK , ELLARD JOHN , ZOLLITSCH THOMAS , BRIGGS MARK SAMUEL JONATHAN
IPC分类号： C08G83/00 , C12Q1/37 , G01N33/58 , C09B11/06 , C09B11/08 , C09B23/08 , C09B69/10
CPC分类号： C08G83/003 , C12Q1/37 , G01N33/582
摘要： A novel form of dendrimer, polybranched molecule, is described in which fluorescent dyes of the structure exist in a micro environment that effects their fluorescent properties. Within the dendrimer, poly-branched molecules are cleavage sites. When these cleavage sites are treated with a suitable chemical or enzyme which cleaves selective bonds the dyes of the structure are released and a change in their optical properties is effected, notably an increase in fluorescent signal. Methods of using the dendrimer, poly-branched molecule in assays of biological molecules are also described.
摘要翻译：描述了一种新型的树枝状大分子多支链分子，其中结构的荧光染料存在于影响其荧光性质的微环境中。在树枝状聚合物内，多分枝分子是切割位点。当用合适的化学物质或裂解选择性键的酶处理这些裂解位点时，结构的染料被释放并且其光学性质发生改变，特别是荧光信号的增加。还描述了在生物分子测定中使用树枝状聚合物，多支链分子的方法。

6. 发明申请

WO02086945A3 DEVICES AND METHODS FOR THE DETECTION OF PARTICLES 审中-公开
标题翻译：用于检测颗粒的装置和方法
公开(公告)号：WO02086945A3
公开(公告)日：2003-04-10
申请号：PCT/GB0201753
申请日：2002-04-19
申请人： AMERSHAM BIOSCIENCES UK LTD , SHARMA SHIV
发明人： SHARMA SHIV
IPC分类号： H01J49/16 , H01J49/40 , H01J49/02
CPC分类号： H01J49/40 , H01J49/025
摘要： The present invention relates to devices and methods for determining the masses of particles by measuring the time between a first event such as a sample (5) being ionised, (or a beam of electromagnetic radiation being scattered by a particle (15) and electromagnetic radiation scattered by said particle being detected by a detection means,) and a second event in which a beam (21) of electromagnetic radiation is scattered by a particle (15) from said ionised sample and electromagnetic radiation (25) from said beam (21) scattered by said particle (15) is detected by a detection means (11).
摘要翻译：本发明涉及通过测量诸如被电离的样品（5）的第一事件（或由粒子（15）散射的电磁辐射束）和电磁辐射之间的时间来确定颗粒质量的装置和方法以及第二事件，其中电磁辐射束（21）被来自所述电离样品的粒子（15）和来自所述光束（21）的电磁辐射（25）散射，通过检测装置（11）检测由所述粒子（15）散射的物体。

7. 发明申请

WO03027677A2 METHOD OF ATTACHMENT 审中-公开
标题翻译：附件方法
公开(公告)号：WO03027677A2
公开(公告)日：2003-04-03
申请号：PCT/GB0204369
申请日：2002-09-26
申请人： AMERSHAM BIOSCIENCES UK LTD , ODEDRA RAJ , PICKERING LEE
发明人： ODEDRA RAJ , PICKERING LEE
IPC分类号： C40B40/10 , G01N33/543 , G01N33/552 , G01N37/00 , C12Q1/68
CPC分类号： C40B40/06 , B01J2219/00533 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00677 , B01J2219/00725 , B82Y30/00 , C07B2200/11 , C40B40/10 , C40B50/18 , G01N33/54353 , G01N33/552
摘要： Disclosed is a method for attaching biomolecules to a solid surface and a composition for preparing that surface for attachment. The composition comprises molecules of Formula I and Formula II which are defined as follows: Y - X - Z - R1 Formula I Y'- X' - Z' - R2 Formula II and wherein R1 is a biomolecule, a reactive group or a group capable of forming a reactive group; R2 is different to R1 and is present in at least a 10 fold molar excess to R1;Y and Y' are groups which can bind to a solid surface; X and X' are atoms which are, at least, bivalent; and Z and Z' are linker groups.
摘要翻译：公开了将生物分子附着到固体表面的方法和用于制备该表面以用于附着的组合物。该组合物包含式I和式II的分子，其定义如下：Y-X-Z-R1式I Y'- X'-Z'-R 2式II，其中R 1是生物分子，反应性基团或基团能够形成反应性基团; R2与R1不同，并且以R1至少存在10×4摩尔摩尔量; Y和Y'可以结合固体表面的基团; X和X'是至少二价的原子; 并且Z和Z'是连接基团。

8. 发明申请

WO2006035207A2 FLUORESCENT NUCLEOTIDE ANALOGUES 审中-公开
标题翻译：荧光核素类似物
公开(公告)号：WO2006035207A2
公开(公告)日：2006-04-06
申请号：PCT/GB2005003685
申请日：2005-09-27
申请人： AMERSHAM BIOSCIENCES UK LTD , BRIGGS MARK SAMUEL JONATHAN , SMITH TONY , SANTOS ALBERT FRANCIS
发明人： BRIGGS MARK SAMUEL JONATHAN , SMITH TONY , SANTOS ALBERT FRANCIS
IPC分类号： C09B23/00
CPC分类号： C09B23/02 , C07H19/10 , C07H19/20
摘要： The present invention provides fluorescent cyanine dye-based nucleotide analogues, in which the cyanine dye is coupled to the -phosphate group of a nucleoside triphosphate. Preferred embodiments of the invention are directed to fluorescent cyanine dye-based GTP analogues which may be employed in an homogeneous FRET-based assay to measure the binding of guanine nucleotides to GPCR polypeptides, or alternatively, to measure the effect of an exogenous ligand on GPCR protein binding.
摘要翻译：本发明提供了基于荧光花青染料的核苷酸类似物，其中花青染料与核苷三磷酸的 - 磷酸基团偶联。本发明的优选实施方案涉及基于荧光花青染料的GTP类似物，其可用于基于FRET的均质测定中以测量鸟嘌呤核苷酸与GPCR多肽的结合，或者可测量外源配体对GPCR的作用蛋白质结合。

9. 发明申请

WO2005080992A3 P-GLYCOPROTEIN ASSAY 审中-公开
标题翻译： P-糖蛋白测定法
公开(公告)号：WO2005080992A3
公开(公告)日：2005-12-08
申请号：PCT/GB2005000614
申请日：2005-02-21
申请人： AMERSHAM BIOSCIENCES UK LTD , BERRY JENNY
发明人： BERRY JENNY
IPC分类号： G01N33/60 , G01N33/68
CPC分类号： G01N33/60 , G01N33/68
摘要： The invention relates to a radio-ligand method for measuring the binding of a test agent to a P-glycoprotein preparation. The invention is a homogeneous scintillation proximity assay for quantifying the binding of a ligand and a test agent to a P-glycoprotein preparation. The assay is suitable for high-throughput screening to identify novel substrates/ inhibitors of P-glycoprotein.
摘要翻译：本发明涉及用于测量测试剂与P-糖蛋白制剂的结合的放射性配体方法。本发明是用于定量配体和测试剂与P-糖蛋白制剂的结合的均相闪烁亲近测定法。该分析适用于高通量筛选以鉴定新的P-糖蛋白基质/抑制剂。

10. 发明申请

WO2005012880B1 ANALYSING BIOLOGICAL ENTITIES 审中-公开
标题翻译：分析生物实体
公开(公告)号：WO2005012880B1
公开(公告)日：2005-03-24
申请号：PCT/GB2004003247
申请日：2004-07-28
申请人： AMERSHAM BIOSCIENCES UK LTD , ARINI NICHOLAS SOLVATORE , GOODYER IAN DAVID , RUEHLMANN DIETRICH OTTO , THOMAS NICHOLAS
发明人： ARINI NICHOLAS SOLVATORE , GOODYER IAN DAVID , RUEHLMANN DIETRICH OTTO , THOMAS NICHOLAS
IPC分类号： G01N1/30 , G01N21/64 , G01N33/487
CPC分类号： G01N1/30 , G01N2021/6439
摘要： A method of analysing a plurality of biological entities using an imaging apparatus. The method comprises: providing a marker for said plurality of biological entities, said marker being capable of identifying objects within said plurality of biological entities when detected using the imaging apparatus, the method of provision being arranged such that said marker is capable of identifying said objects during a first time period, and said marker is less capable of identifying said objects during a second time period; during the first time period, recording a marked-up image in which spatial definitions of said objects are identifiable from said marker; during the second time period, recording a first image of said plurality of biological entities; and generating a spatial definition for an object in said first image using data derived from said marked-up image.
摘要翻译：一种使用成像装置分析多个生物实体的方法。该方法包括：用于所述多个生物实体提供标记，所述标记能够识别对象的内多个生物实体使用所述成像装置检测到时，被布置在所述规定的方法，使得所述标记物能够识别所述的对象在第一时间段期间，并且所述标记在第二时间段期间不太能够识别所述物体; 在第一时间段期间，记录标记图像，其中所述对象的空间定义可从所述标记中识别; 在第二时间段期间，记录所述多个生物实体的第一图像; 以及使用从所述标记图像导出的数据来生成所述第一图像中的对象的空间定义。

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