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1. 发明申请

WO2006061640A2 METHOD OF, AND APPARATUS AND COMPUTER SOFTWARE FOR IMAGING BIOLOGICAL OBJECTS 审中-公开
标题翻译：用于成像生物对象的方法，装置和计算机软件
公开(公告)号：WO2006061640A2
公开(公告)日：2006-06-15
申请号：PCT/GB2005/004747
申请日：2005-12-09
申请人： GE HEALTHCARE UK LIMITED , ADIE, Elaine, J. , ARINI, Nicholas, Solvatore
发明人： ADIE, Elaine, J. , ARINI, Nicholas, Solvatore
IPC分类号： G01N15/14 , G01N21/03 , G01N21/64 , G06K9/00 , G06T5/00
CPC分类号： G01N21/6456 , G01N15/1475 , G01N21/6452 , G01N21/6486 , G06K9/00127 , G06T7/11 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： A method of imaging one or more biological objects using imaging apparatus capable of capturing an image across an imaging area. The method includes: placing the one or more biological objects (236) in an environment; providing in the environment, outside of the one or more biological objects, a contrast enhancing agent which provides contrast in an image between the one or more biological objects and the environment; and recording an image (240) of the one or more biological objects and the environment using the imaging apparatus, whereby a spatial definition for said one or more biological objects is derivable using contrast in the image which is provided by the contrast enhancing agent.
摘要翻译：使用能够在成像区域捕获图像的成像装置对一个或多个生物体进行成像的方法。该方法包括：将一个或多个生物体（236）放置在环境中; 在所述环境中提供所述一种或多种生物体之外的对比度增强剂，其在所述一种或多种生物体与所述环境之间的图像中提供对比度; 以及使用所述成像装置记录所述一个或多个生物体和环境的图像（240），从而可以使用所述对比度增强剂提供的图像中的对比度来导出所述一个或多个生物体的空间定义。

2. 发明申请

WO2006061640A3 METHOD OF, AND APPARATUS AND COMPUTER SOFTWARE FOR IMAGING BIOLOGICAL OBJECTS 审中-公开
标题翻译：用于成像生物对象的方法，装置和计算机软件
公开(公告)号：WO2006061640A3
公开(公告)日：2007-02-22
申请号：PCT/GB2005004747
申请日：2005-12-09
申请人： GE HEALTHCARE UK LTD , ADIE ELAINE J , ARINI NICHOLAS SOLVATORE
发明人： ADIE ELAINE J , ARINI NICHOLAS SOLVATORE
IPC分类号： G01N15/14 , G01N21/03 , G01N21/64 , G01N33/50 , G06K9/00 , G06T5/00
CPC分类号： G01N21/6456 , G01N15/1475 , G01N21/6452 , G01N21/6486 , G06K9/00127 , G06T7/11 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： A method of imaging one or more biological objects using imaging apparatus capable of capturing an image across an imaging area. The method includes: placing the one or more biological objects (236) in an environment; providing in the environment, outside of the one or more biological objects, a contrast enhancing agent which provides contrast in an image between the one or more biological objects and the environment; and recording an image (240) of the one or more biological objects and the environment using the imaging apparatus, whereby a spatial definition for said one or more biological objects is derivable using contrast in the image which is provided by the contrast enhancing agent.
摘要翻译：使用能够在成像区域捕获图像的成像装置对一个或多个生物体进行成像的方法。该方法包括：将一个或多个生物体（236）放置在环境中; 在所述环境中提供所述一种或多种生物体之外的对比度增强剂，其在所述一种或多种生物体与所述环境之间的图像中提供对比度; 以及使用所述成像装置记录所述一个或多个生物体和环境的图像（240），从而可以使用所述对比度增强剂提供的图像中的对比度来导出所述一个或多个生物体的空间定义。

3. 发明申请

WO2005012880B1 ANALYSING BIOLOGICAL ENTITIES 审中-公开
标题翻译：分析生物实体
公开(公告)号：WO2005012880B1
公开(公告)日：2005-03-24
申请号：PCT/GB2004003247
申请日：2004-07-28
申请人： AMERSHAM BIOSCIENCES UK LTD , ARINI NICHOLAS SOLVATORE , GOODYER IAN DAVID , RUEHLMANN DIETRICH OTTO , THOMAS NICHOLAS
发明人： ARINI NICHOLAS SOLVATORE , GOODYER IAN DAVID , RUEHLMANN DIETRICH OTTO , THOMAS NICHOLAS
IPC分类号： G01N1/30 , G01N21/64 , G01N33/487
CPC分类号： G01N1/30 , G01N2021/6439
摘要： A method of analysing a plurality of biological entities using an imaging apparatus. The method comprises: providing a marker for said plurality of biological entities, said marker being capable of identifying objects within said plurality of biological entities when detected using the imaging apparatus, the method of provision being arranged such that said marker is capable of identifying said objects during a first time period, and said marker is less capable of identifying said objects during a second time period; during the first time period, recording a marked-up image in which spatial definitions of said objects are identifiable from said marker; during the second time period, recording a first image of said plurality of biological entities; and generating a spatial definition for an object in said first image using data derived from said marked-up image.
摘要翻译：一种使用成像装置分析多个生物实体的方法。该方法包括：用于所述多个生物实体提供标记，所述标记能够识别对象的内多个生物实体使用所述成像装置检测到时，被布置在所述规定的方法，使得所述标记物能够识别所述的对象在第一时间段期间，并且所述标记在第二时间段期间不太能够识别所述物体; 在第一时间段期间，记录标记图像，其中所述对象的空间定义可从所述标记中识别; 在第二时间段期间，记录所述多个生物实体的第一图像; 以及使用从所述标记图像导出的数据来生成所述第一图像中的对象的空间定义。

4. 发明申请

WO2005012880A1 ANALYSING BIOLOGICAL ENTITIES 审中-公开
标题翻译：分析生物实体
公开(公告)号：WO2005012880A1
公开(公告)日：2005-02-10
申请号：PCT/GB2004/003247
申请日：2004-07-28
申请人： AMERSHAM BIOSCIENCES UK LIMITED , ARINI, Nicholas, Solvatore , GOODYER, Ian, David , RUEHLMANN, Dietrich, Otto , THOMAS, Nicholas
发明人： ARINI, Nicholas, Solvatore , GOODYER, Ian, David , RUEHLMANN, Dietrich, Otto , THOMAS, Nicholas
IPC分类号： G01N1/30
CPC分类号： G01N1/30 , G01N2021/6439
摘要： A method of analysing a plurality of biological entities using an imaging apparatus. The method comprises: providing a marker for said plurality of biological entities, said marker being capable of identifying objects within said plurality of biological entities when detected using the imaging apparatus, the method of provision being arranged such that said marker is capable of identifying said objects during a first time period, and said marker is less capable of identifying said objects during a second time period; during the first time period, recording a marked-up image in which spatial definitions of said objects are identifiable from said marker; during the second time period, recording a first image of said plurality of biological entities; and generating a spatial definition for an object in said first image using data derived from said marked-up image.
摘要翻译：使用成像装置分析多个生物实体的方法。该方法包括：提供用于所述多个生物实体的标记，当使用成像装置检测时，所述标记能够识别所述多个生物实体内的物体，所述提供方法被布置为使得所述标记能够识别所述物体并且所述标记在第二时间期间不太能够识别所述对象; 在第一时段期间，记录标记图像，其中所述对象的空间定义可从所述标记识别; 在所述第二时间段期间，记录所述多个生物实体的第一图像; 以及使用从所述标记图像导出的数据来生成所述第一图像中的对象的空间定义。

5. 发明申请

WO2004088573A1 DETERMINING CELL CYCLE PHASE DATA 审中-公开
标题翻译：确定细胞周期数据
公开(公告)号：WO2004088573A1
公开(公告)日：2004-10-14
申请号：PCT/GB2003/003876
申请日：2003-09-08
申请人： AMERSHAM BIOSCIENCES UK LIMITED , ARINI, Nicholas, Solvatore , GOODYER, Ian, David , ZALTSMAN, Alla, Borisovna
发明人： ARINI, Nicholas, Solvatore , GOODYER, Ian, David , ZALTSMAN, Alla, Borisovna
IPC分类号： G06K9/00
CPC分类号： G01N21/6452 , G01N15/1468 , G01N21/6428 , G01N21/6458 , G01N2015/1477 , G01N2015/1493 , G06K9/00127
摘要： A method of determining cell cycle phase data for cells comprising at least one luminescent reporter capable of emitting radiation, the at least one luminescent reporter comprising a first luminescent reporter which is capable of being indicative of at least one cell cycle phase, said method comprising: storing classification rules information for classifying individual cells into different cell cycle phases using an automated classification process; receiving image data created by detecting radiation emitted by said at least one luminescent reporter; analyzing said image data to identify object areas in the image data which correspond to individual cells; analyzing said image data, on the basis of said identified object areas, to determine, for a selected cell, one or more measurements including a measurement of a parameter relating to at least a cytoplasmic component of the cell; and applying said classification rules information to said measurements to classify the selected cell into a selected one of a plurality of sub-populations of cells, each sub-population having cells in a different cell cycle phase.
摘要翻译：一种确定细胞的细胞周期相位数据的方法，所述细胞包含至少一种能够发射辐射的发光报告基团，所述至少一个发光报告基因包含能够指示至少一个细胞周期相的第一发光报道基因，所述方法包括：存储用于使用自动分类过程将各个细胞分类成不同的细胞周期阶段的分类规则信息; 接收通过检测由所述至少一个发光报告器发射的辐射而产生的图像数据; 分析所述图像数据以识别对应于各个单元的图像数据中的对象区域; 基于所述识别的对象区域分析所述图像数据，以针对所选择的小区来确定包括与所述小区的至少细胞质组分相关的参数的测量的一个或多个测量; 以及将所述分类规则信息应用于所述测量以将所选择的细胞分类成多个细胞子群体中的所选择的一个，每个子群体具有不同细胞周期阶段的细胞。

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