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21. 发明申请

US20100051459A1 Denaturant-Free Electrophoresis of Biological Molecules Under High Temperature Conditions 审中-公开
标题翻译：高温条件下生物分子的无变性免疫电泳
公开(公告)号：US20100051459A1
公开(公告)日：2010-03-04
申请号：US12349448
申请日：2009-01-06
申请人： Qingbo Li , Kevin J. Levan , Heide Monroe
发明人： Qingbo Li , Kevin J. Levan , Heide Monroe
IPC分类号： B01D57/02 , G01N27/26
CPC分类号： G01N27/44704 , G01N27/44747
摘要： The present invention relates to a method of separating a sample comprising biological compounds, such as nucleic acids. The nucleic acids are subjected to electrophoresis using a matrix that is essentially free of denaturants and having at least one random, linear copolymer comprising a first comonomer of acrylamide and at least one secondary comonomer. A temperature of at least a portion of the matrix is at least about 80° C.
摘要翻译：本发明涉及分离包含生物化合物如核酸的样品的方法。使用基本上不含变性剂并具有至少一种包含丙烯酰胺的第一共聚单体和至少一种仲共聚单体的无规线性共聚物进行电泳的核酸。基质的至少一部分的温度为至少约80℃

22. 发明申请

US20080228083A1 Non-Evasive Method and Apparatus of Detection of Organism Tissues 失效
标题翻译：非侵入性方法和器官检测器具组织
公开(公告)号：US20080228083A1
公开(公告)日：2008-09-18
申请号：US10586272
申请日：2005-01-18
申请人： Jinguang Wu , Yuanfu Zhang , Yizhuang Xu , Qingbo Li , Shifu Weng , Limin Yang , Li Zhang , Weijin Zhou , Zhanian Yang , Weihong Li , Huizhong Wu , Rongye Gong , Xuejun Gao , Duanfu Xu , Ying Zhao , Zhi Xu , Nengwei Zhang , Lixin Wang , Kaihua Sun , Xin Peng , Yufeng Liu , Xuejun Sun , Xiaofeng Ling , Su Zhou , Zhi Liu , Guoqiao Lai , Jianxiong Jiang , Qinghua Pan , Qiguang Wu , Guangyan Yu , Bingbing Wang
发明人： Jinguang Wu , Yuanfu Zhang , Yizhuang Xu , Qingbo Li , Shifu Weng , Limin Yang , Li Zhang , Weijin Zhou , Zhanian Yang , Weihong Li , Huizhong Wu , Rongye Gong , Xuejun Gao , Duanfu Xu , Ying Zhao , Zhi Xu , Nengwei Zhang , Lixin Wang , Kaihua Sun , Xin Peng , Yufeng Liu , Xuejun Sun , Xiaofeng Ling , Su Zhou , Zhi Liu , Guoqiao Lai , Jianxiong Jiang , Qinghua Pan , Qiguang Wu , Guangyan Yu , Bingbing Wang
IPC分类号： A61B6/00 , G01J5/02
CPC分类号： A61B5/0059 , A61B5/6848 , A61B5/7257
摘要： The invention relates to a non-invasive method to diagnose the changes of molecular structures of organism tissues from body surface and a dedicated apparatus. The apparatus is comprised of a Fourier Transform infrared spectrometer and a set of additional accessories. Said additional accessories include a mid-IR fiber optics sampling attachment, a fiber coupling part, and an infrared detector part. The detection method is comprised of placing the ATR probe of the dedicated apparatus on the skin surface of a region to be tested, and scanning more than one time in which the resolution of the apparatus is 1-32 cm−1 and the range of the spectrum is 800-4000 cm−1. It is possible to detect changes in molecular structures of living biological tissues in the early stages of cancer, and testees will not feel uncomfortable during testing. The method is easy to operate, quick, accurate, and it doesn't harm the body.
摘要翻译：本发明涉及用于诊断来自身体表面的生物体组织的分子结构的变化的非侵入性方法和专用设备。该装置由傅里叶变换红外光谱仪和一组附加附件组成。所述附加附件包括中红外光纤采样附件，光纤耦合部件和红外探测器部件。检测方法包括将专用装置的ATR探针放置在待测试区域的皮肤表面上，扫描多于一次，其中装置的分辨率为1-32cm -1， SUP>，光谱范围为800-4000cm -1。在癌症早期阶段可以检测生物体组织的分子结构变化，受试者在测试过程中不会感到不适。该方法易于操作，快速，准确，不伤身体。

23. 发明授权

US07175750B2 System and method for temperature gradient capillary electrophoresis 有权
标题翻译：温度梯度毛细管电泳的系统和方法
公开(公告)号：US07175750B2
公开(公告)日：2007-02-13
申请号：US10287826
申请日：2002-11-05
申请人： Zhiyong Guo , Zhaowei Liu , Qingbo Li
发明人： Zhiyong Guo , Zhaowei Liu , Qingbo Li
IPC分类号： G01N27/447
CPC分类号： G01N27/44773 , C12Q1/6816 , C12Q1/6827 , C12Q2600/156 , G01N27/44704 , G01N27/44721 , C12Q2565/125 , C12Q2545/113 , C12Q2527/101 , C12Q2565/131 , C12Q2527/107
摘要： The present invention relates to a method for determining the presence of a mutation in a first sample comprising first nucleotides. The reference sample comprising reference nucleotides. The first sample and a reference sample are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first sample and the reference sample is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference nucleotides. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference nucleotides. At least one of the first sample or reference samples comprises products resulting from a polymerase chain reaction (PCR), the products not having been desalted prior to electrophoresis.
摘要翻译：本发明涉及用于确定包含第一核苷酸的第一样品中突变的存在的方法。参考样品包含参考核苷酸。第一个样品和参考样品在至少一个插层染料存在下进行电泳。在电泳期间，第一样品和参考样品的温度改变足以改变至少一个第一个或参考核苷酸的电泳迁移率的量。获得荧光强度数据。荧光强度数据表明第一个和参考核苷酸的存在。第一个样本或参考样本中的至少一个包含由聚合酶链反应（PCR）产生的产物，该产物在电泳之前未脱盐。

24. 发明授权

US06926815B2 Copolymers for capillary gel electrophoresis 有权
标题翻译：用于毛细管凝胶电泳的共聚物
公开(公告)号：US06926815B2
公开(公告)日：2005-08-09
申请号：US09843918
申请日：2001-04-30
申请人： Changsheng Liu , Qingbo Li
发明人： Changsheng Liu , Qingbo Li
IPC分类号： C08F220/56 , G01N27/447 , G01N27/441 , G01N27/453
CPC分类号： G01N27/44747
摘要： This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.
摘要翻译：本发明涉及一种具有至少一种含有主要共聚单体和至少一种次级共聚单体的无规线性共聚物的凝胶基质的电泳分离介质，其中共聚单体沿共聚物链无规分布。主要共聚单体是提供凝胶基质的主要物理，化学和筛分性质的丙烯酰胺或丙烯酰胺衍生物。至少一种次级共聚单体赋予共聚物链固有的物理，化学或筛分特性。初级和次级共聚单体以足以引发优化电泳性能的所需性质的比例存在。本发明还涉及使用该凝胶基质分离生物分子的混合物的方法，制备新型电泳分离介质的方法和填充有电泳分离介质的毛细管。

25. 发明授权

US06630063B1 Uniform laser excitation and detection in capillary array electrophoresis system and method 有权
标题翻译：毛细管阵列电泳系统和方法的均匀激光激发和检测
公开(公告)号：US06630063B1
公开(公告)日：2003-10-07
申请号：US09413355
申请日：1999-10-06
申请人： Qingbo Li , Songsan Zhou , Changsheng Liu
发明人： Qingbo Li , Songsan Zhou , Changsheng Liu
IPC分类号： G01N2726
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary electrophoresis system comprises capillaries positioned in parallel to each other forming a plane. The capillaries are configured to allow samples to migrate. A light source is configured to illuminate the capillaries and the samples therein. This causes the samples to emit light. A lens is configured to receive the light emitted by the samples and positioned directly over a first group of the capillaries and obliquely over a second group of the capillaries. The light source is further configured to illuminate the second group of capillaries more than the first group of the capillaries such that amount of light received by the lens from the first group of capillaries is substantially identical to amount of light received from the second group of capillaries when an identical amount of the samples is migrating through the first and second group capillaries.
摘要翻译：毛细管电泳系统包括彼此平行地形成平面的毛细管。毛细管被配置为允许样品迁移。光源被配置为照亮毛细管和其中的样品。这样会使样品发光。透镜被配置为接收由样品发射的光并且直接定位在第一组毛细管上并且倾斜地位于第二组毛细管上。光源还被配置为比第一组毛细管照亮第二组毛细管，使得透镜从第一组毛细管接收的光量与从第二组毛细血管接收的光量基本相同当相同量的样品通过第一和第二组毛细管迁移时。

26. 发明授权

US6063251A Electrically insulated capillary arrays for electrophoretic applications 失效
标题翻译：用于电泳应用的电绝缘毛细管阵列
公开(公告)号：US6063251A
公开(公告)日：2000-05-16
申请号：US866308
申请日：1997-05-30
申请人： Thomas E. Kane , John R. Kernan , Changsheng Liu , Qingbo Li
发明人： Thomas E. Kane , John R. Kernan , Changsheng Liu , Qingbo Li
IPC分类号： G01N27/447
CPC分类号： G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. Electrical crosstalk between neighboring capillaries is attenuated using a protective tubing formed from an electrically insulative material. This crosstalk attenuation is provided in the form of sheathing, either around individual capillaries, or around bundles of spaced apart capillaries. Crosstalk attenuation may be enhanced by passing a nonconductive gas or liquid through lumens formed in the protective tubing, in which lumens the capillary tubes are present.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。使用由电绝缘材料形成的保护管，相邻毛细管之间的电气串扰被衰减。这种串扰衰减以围绕单个毛细管或围绕分开的毛细管束的护套形式提供。可以通过使非导电气体或液体通过在保护管中形成的内腔（其中存在毛细管的管腔）来增强串扰衰减。

27. 发明授权

US6027627A Automated parallel capillary electrophoretic system 失效
标题翻译：自动并行毛细管电泳系统
公开(公告)号：US6027627A
公开(公告)日：2000-02-22
申请号：US105988
申请日：1998-06-29
申请人： Qingbo Li , Thomas E. Kane , Changsheng Liu , Bernard Sonnenschein , Michael V. Sharer , John R. Kernan
发明人： Qingbo Li , Thomas E. Kane , Changsheng Liu , Bernard Sonnenschein , Michael V. Sharer , John R. Kernan
IPC分类号： G01N27/447 , G01N27/26 , G01N27/477
CPC分类号： G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

28. 发明授权

US5998796A Detector having a transmission grating beam splitter for multi-wavelength sample analysis 失效
公开(公告)号：US5998796A
公开(公告)日：1999-12-07
申请号：US84236
申请日：1998-05-26
申请人： Changsheng Liu , Qingbo Li
发明人： Changsheng Liu , Qingbo Li
IPC分类号： G01N21/64 , G01N27/447
CPC分类号： G01N27/44721
摘要： A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS). The TGBS split fluoresced light from a tagged DNA sample into 0th order and a 1st order components, both of which are detected on a two-dimensional detector array of a CCD camera. The 0th and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The TGBS splits fluoresced incoming light into 0th and 1st order components, which are then spread out among a number of pixels in the detector array. The 1st component of this light is received by pixels whose position relative to the 0th order component depends on the frequency of fluorescence. Thus, the position at which signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the 0th order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.

29. 发明授权

US5741411A Multiplexed capillary electrophoresis system 失效
公开(公告)号：US5741411A
公开(公告)日：1998-04-21
申请号：US665800
申请日：1996-06-18
申请人： Edward S. Yeung , Qingbo Li , Xiandan Lu
发明人： Edward S. Yeung , Qingbo Li , Xiandan Lu
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721
摘要： The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

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