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1. 发明授权

US5582705A Multiplexed capillary electrophoresis system 失效
标题翻译：多重毛细管电泳系统
公开(公告)号：US5582705A
公开(公告)日：1996-12-10
申请号：US444565
申请日：1995-05-19
申请人： Edward S. Yeung , Huan-Tsang Chang , Eliza N. Fung , Qingbo Li , Xiandan Lu
发明人： Edward S. Yeung , Huan-Tsang Chang , Eliza N. Fung , Qingbo Li , Xiandan Lu
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721
摘要： The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.
摘要翻译：本发明提供了用于多路复用毛细管电泳系统中的侧入光激发几何形状。电荷注入装置光学耦合到阵列中的毛细管，使得毛细管的内部成像到仅一个像素上。在Sanger型4标记DNA测序反应中，通过使用两个长通滤波器将荧光发射分解成两个发射通道来改善核苷酸鉴定（“碱基呼叫”）。在电泳分离中使用二元聚（环氧乙烷）基质。

2. 发明授权

US5741411A Multiplexed capillary electrophoresis system 失效
公开(公告)号：US5741411A
公开(公告)日：1998-04-21
申请号：US665800
申请日：1996-06-18
申请人： Edward S. Yeung , Qingbo Li , Xiandan Lu
发明人： Edward S. Yeung , Qingbo Li , Xiandan Lu
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721
摘要： The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

3. 发明申请

US20090112487A1 Vehicle dynamic measurement device and method for comprehensive parameters of rail wear 有权
标题翻译：轨道磨损综合参数车辆动态测量装置及方法
公开(公告)号：US20090112487A1
公开(公告)日：2009-04-30
申请号：US12258398
申请日：2008-10-25
申请人： Guangjun Zhang , Junhua Sun , Zhenzhong Wei , Fuqiang Zhou , Qingbo Li , Zhen Liu , Qianzhe Liu
发明人： Guangjun Zhang , Junhua Sun , Zhenzhong Wei , Fuqiang Zhou , Qingbo Li , Zhen Liu , Qianzhe Liu
IPC分类号： G01B11/00
CPC分类号： G01B11/2513 , B61K9/08 , G01B11/2522
摘要： The invention discloses a vehicle dynamic measurement device for comprehensive parameters of rail wear, which comprises a vision sensor, a computer and a milometer. A high-speed image acquisition card and a measurement module are installed in the computer. The vision sensor comprises imaging system for rail cross-section and a raster projector which can project more than one light plane perpendicular to the measured rail. The measurement module is used for calculating vertical wear, horizontal wear, the amplitude and wavelength of corrugation wear. The invention also discloses a vehicle dynamic measurement method for comprehensive parameters of rail wear. The invention can increase the sampling rate of image sensing and acquisition hardware equipment with no need of improving the performance of it, thereby satisfy high-speed on-line dynamic measurement requirements for corrugation wear, and the amplitude and wavelength of corrugation wear can be calculated more precisely.
摘要翻译：本发明公开了一种用于轨道磨损综合参数的车辆动态测量装置，其包括视觉传感器，计算机和千米。计算机中安装有高速图像采集卡和测量模块。视觉传感器包括用于轨道横截面的成像系统和光栅投影仪，该投影仪可以投影多个垂直于测量轨道的光平面。测量模块用于计算垂直磨损，水平磨损，波纹磨损的振幅和波长。本发明还公开了一种用于铁路磨损综合参数的车辆动态测量方法。本发明可以增加图像感测和采集硬件设备的采样率，不需要提高其性能，从而满足波纹磨损的高速在线动态测量要求，并且可以计算波纹磨损的幅度和波长更确切地说。

4. 发明授权

US07473341B2 Denaturant-free electrophoresis of biological molecules under high temperature conditions 有权
标题翻译：在高温条件下生物分子的无变性电泳
公开(公告)号：US07473341B2
公开(公告)日：2009-01-06
申请号：US10661558
申请日：2003-09-15
申请人： Qingbo Li , Kevin J. Levan , Heidi Monroe
发明人： Qingbo Li , Kevin J. Levan , Heidi Monroe
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44747
摘要： The present invention relates to a method of separating a sample comprising biological compounds, such as nucleic acids. The nucleic acids are subjected to electrophoresis using a matrix that is essentially free of denaturants and having at least one random, linear copolymer comprising a first comonomer of acrylamide and at least one secondary comonomer. A temperature of at least a portion of the matrix is at least about 80° C.
摘要翻译：本发明涉及分离包含生物化合物如核酸的样品的方法。使用基本上不含变性剂并具有至少一种包含丙烯酰胺的第一共聚单体和至少一种仲共聚单体的无规线性共聚物进行电泳的核酸。基质的至少一部分的温度为至少约80℃

5. 发明申请

US20060091011A1 Automated parallel capillary electrophoresis system with hydrodynamic sample injection 有权
公开(公告)号：US20060091011A1
公开(公告)日：2006-05-04
申请号：US11204773
申请日：2005-08-15
申请人： Changsheng Liu , Thomas Kane , Qingbo Li
发明人： Changsheng Liu , Thomas Kane , Qingbo Li
IPC分类号： C07K1/26 , G01N27/447
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44743 , G01N27/44782 , Y10T436/2575
摘要： An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

6. 发明授权

US06375819B1 Fluid delivery system for capillary electrophoretic applications 失效
标题翻译：用于毛细管电泳应用的流体输送系统
公开(公告)号：US06375819B1
公开(公告)日：2002-04-23
申请号：US09373909
申请日：1999-08-13
申请人： Qingbo Li , Changsheng Liu , Thomas E. Kane , John R. Kernan , Bernard Sonnenschein , Michael V. Sharer
发明人： Qingbo Li , Changsheng Liu , Thomas E. Kane , John R. Kernan , Bernard Sonnenschein , Michael V. Sharer
IPC分类号： G01N2726
CPC分类号： G01N27/44721 , G01N27/44782
摘要： An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
摘要翻译：公开了一种自动电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

7. 发明申请

US20090090630A1 Automated parallel capillary electrophoresis system with hydrodynamic sample injection 审中-公开
标题翻译：自动平行毛细管电泳系统与流体动力学样品注射
公开(公告)号：US20090090630A1
公开(公告)日：2009-04-09
申请号：US12290087
申请日：2008-10-27
申请人： Changsheng Liu , Thomas E. Kane , Qingbo Li
发明人： Changsheng Liu , Thomas E. Kane , Qingbo Li
IPC分类号： G01N27/453
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44743 , G01N27/44782 , Y10T436/2575
摘要： An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends is spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresed into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.
摘要翻译：公开了一种自动化毛细管区域电泳系统。该系统采用具有多个毛细管的毛细管盒。盒具有从板的一侧突出的第一阵列的毛细管端。毛细管端部的第一阵列以与标准尺寸的微量滴定塔的孔基本相同的方式间隔开。这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。该系统还具有连接到毛细管的检测端的容器。该容器设置有阀门，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

8. 发明申请

US20050064400A1 System and method for temperature gradient capillary electrophoresis 审中-公开
标题翻译：温度梯度毛细管电泳的系统和方法
公开(公告)号：US20050064400A1
公开(公告)日：2005-03-24
申请号：US10287808
申请日：2002-11-05
申请人： Zhiyong Guo , Zhaowei Liu , Qingbo Li
发明人： Zhiyong Guo , Zhaowei Liu , Qingbo Li
IPC分类号： C12Q1/68 , C12Q1/6827 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12Q1/6827 , C12Q2565/125 , C12Q2527/101
摘要： The present invention relates to a method for determining the presence of a mutation in a first sample comprising first polynucleotides. The reference sample comprises reference polynucleotides. The first sample and a reference sample are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first sample and the reference sample is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference polynucleotides. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference polynucleotides. The data are processed to determine the presence of mutation in the first polynucleotides.
摘要翻译：本发明涉及一种用于确定包含第一多核苷酸的第一样品中突变存在的方法。参考样品包括参考多核苷酸。第一个样品和参考样品在至少一个插层染料存在下进行电泳。在电泳期间，第一样品和参考样品的温度改变足以改变至少一个第一或参考多核苷酸的电泳迁移率的量。获得荧光强度数据。荧光强度数据表明第一和参考多核苷酸的存在。处理数据以确定第一多核苷酸中突变的存在。

9. 发明申请

US20170188974A1 Evaluating multivariate response of circadian rhythms 审中-公开
公开(公告)号：US20170188974A1
公开(公告)日：2017-07-06
申请号：US15115690
申请日：2015-02-03
申请人： Qingbo Li
发明人： Qingbo Li
IPC分类号： A61B5/00 , A61B5/145 , A61B5/0205
CPC分类号： A61B5/7275 , A61B5/0205 , A61B5/021 , A61B5/145 , A61B5/14532 , A61B5/4857 , G16H20/00 , G16H50/30 , G16H50/50
摘要： A statistical system is disclosed to analyze multivariate response of circadian rhythms in a crossover design, in which response variables are continuously monitored to evaluate the therapeutic effect of a regimen on circadian rhythms such as blood pressure and blood sugar. The methods determine the alteration of not only amplitude but also correlation of multiple circadian rhythms under the influence of a regimen.

10. 发明授权

US07329333B2 Uniform laser excitation and detection in capillary array electrophoresis system and method 失效
标题翻译：毛细管阵列电泳系统和方法的均匀激光激发和检测
公开(公告)号：US07329333B2
公开(公告)日：2008-02-12
申请号：US10679514
申请日：2003-10-07
申请人： Qingbo Li , Songsan Zhou , Changsheng Liu
发明人： Qingbo Li , Songsan Zhou , Changsheng Liu
IPC分类号： G01N27/26
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A capillary electrophoresis system comprises capillaries positioned in parallel to each other forming a plane. The capillaries are configured to allow samples to migrate. A light source is configured to illuminate the capillaries and the samples therein. This causes the samples to emit light. A lens is configured to receive the light emitted by the samples and positioned directly over a first group of the capillaries and obliquely over a second group of the capillaries. The light source is further configured to illuminate the second group of capillaries more than the first group of the capillaries such that amount of light received by the lens from the first group of capillaries is substantially identical to amount of light received from the second group of capillaries when an identical amount of the samples is migrating through the first and second group capillaries.
摘要翻译：毛细管电泳系统包括彼此平行地形成平面的毛细管。毛细管被配置为允许样品迁移。光源被配置为照亮毛细管和其中的样品。这样会使样品发光。透镜被配置为接收由样品发射的光并且直接定位在第一组毛细管上并且倾斜地位于第二组毛细管上。光源还被配置为比第一组毛细管照亮第二组毛细管，使得透镜从第一组毛细管接收的光量与从第二组毛细血管接收的光量基本相同当相同量的样品通过第一和第二组毛细管迁移时。

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