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1. 发明申请

WO2018050300A1 POLYSACCHARIDE-OXIDIZING COMPOSITION AND USES THEREOF 审中-公开
标题翻译：多糖 - 氧化组合物及其用途
公开(公告)号：WO2018050300A1
公开(公告)日：2018-03-22
申请号：PCT/EP2017/064414
申请日：2017-06-13
申请人： INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA) , UNIVERSITE AIX MARSEILLE , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
发明人： BERRIN, Jean-Guy , COUTURIER, Marie , HENRISSAT, Bernard , ROSSO, Marie-Noelle , SULZENBACHER, Gerlind , LADEVEZE, Simon
IPC分类号： C12P19/02 , C07K14/37 , C12F3/02 , C12N9/24 , C12P3/00 , C12P7/02 , C12P7/26 , C12P7/40 , C12P13/04 , C12N9/04 , C12P7/14 , C12P7/10
CPC分类号： C12P19/14 , C12F3/02 , C12N9/0006 , C12N9/24 , C12N15/81 , C12P7/10 , C12P19/02 , C12P19/04 , C12P2203/00 , Y02E50/16
摘要： The invention relates to a polysaccharide-oxidizing composition comprising a polysaccharide-oxidizing enzyme wherein, when the said polysaccharide-oxidizing enzyme is compared to the reference polypeptide of SEQ ID NO. 1 as described in the specification by using the BLAST-P comparison method, (i) the said polysaccharide- oxidizing enzyme possesses an amino acid identity of 20 % or more with the said reference polypeptide and (ii) the BLAST-P comparison method results in an E-value of 10 e -3 or less.
摘要翻译：
本发明涉及一种多糖 - 氧化组合物包含多糖氧化酶，其特征在于，当所述多糖氧化酶与SEQ ID NO：2的参照多肽。 1如通过使用BLAST-P的比较方法中，（i）在本说明书中所描述的，所述氧化多糖酶具有20％以上的氨基酸同一性与所述参照多肽和（ii）的BLAST-P的比较方法的结果 E值为10 e -3或更低。

2. 发明申请

WO2018027181A1 PRODUCING AND ALTERING MICROBIAL FERMENTATION PRODUCTS USING NON-COMMONLY USED LIGNOCELLULOSIC HYDROLYSATES 审中-公开
标题翻译：使用非共同使用的木质纤维素水解产物生产和改变微生物发酵产品
公开(公告)号：WO2018027181A1
公开(公告)日：2018-02-08
申请号：PCT/US2017/045595
申请日：2017-08-04
申请人： KUEHNLE AGROSYSTEMS, INC.
发明人： NOLASCO, Norie Anne, B. , VASAVADA, Amit , KUEHNLE, Adelheid, R. , SCHURR, Robert, J.
IPC分类号： C12N1/22 , C12N1/12 , C12P19/02 , C12P19/14 , C12P7/64
CPC分类号： C12P7/065 , C08H8/00 , C12N1/12 , C12N1/22 , C12P1/00 , C12P7/04 , C12P7/6409 , C12P7/6427 , C12P19/02 , C12P19/14 , C12P21/02 , C12P23/00 , C12P39/00 , C12P2203/00 , Y02E50/17
摘要： The invention pertains to a method for synthesizing a product of interest by culturing a microbe that produces the product of interest, the method comprising culturing the microbe in a culture medium, wherein the culture medium is produced by a method comprising the steps of: a) providing a lignocellulosic biomass, b) hydrolyzing the lignocellulosic biomass to produce a lignocellulosic hydrolysate comprising a simplified sugar produced from at least a portion of the lignocellulosic compound, c) optionally, treating a portion of the lignocellulosic hydrolysate to convert a portion of the lignocellulosic compound and/or the simplified sugar to a non-sugar agent; d) optionally, mixing the treated portion of the lignocellulosic hydrolysate, if produced, with the untreated portion of the lignocellulosic hydrolysate, e) producing a culture medium comprising the lignocellulosic hydrolysate obtained after step b) or comprising the mixture obtained after steps c) and d).
摘要翻译：本发明涉及通过培养产生感兴趣产物的微生物来合成感兴趣产物的方法，所述方法包括在培养基中培养微生物，其中所述培养基由方法，所述方法包括以下步骤：a）提供木质纤维素生物质，b）水解木质纤维素生物质以产生包含由至少一部分木质纤维素化合物产生的简化糖的木质纤维素水解产物，c）任选地，将一部分木质纤维素化合物和/或简化糖转化为非糖剂; d）任选地，将木质纤维素水解产物的处理部分（如果产生）与木质纤维素水解产物的未处理部分混合，e）产生培养基，其包含步骤b）之后获得的木质纤维素水解产物或包含步骤c）和 d）。

3. 发明申请

WO2017205705A1 SYSTEMS AND METHODS FOR CONTINUOUSLY FERMENTING C5 AND C6 SACCHARIDES 审中-公开
标题翻译：用于连续引发C5和C6糖链的系统和方法
公开(公告)号：WO2017205705A1
公开(公告)日：2017-11-30
申请号：PCT/US2017/034596
申请日：2017-05-26
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： SURVASE, Shrinkant , ZEBROSKI, Ryan , ADAMOS, Georgios , PYLKKANEN, Vesa
IPC分类号： C12P7/04 , C12P7/06 , C12P7/14 , C12P7/16 , C12P7/28 , C12Q3/00
CPC分类号： C12P7/10 , C12P7/04 , C12P7/16 , C12P7/28 , C12P7/52 , C12P7/54 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C 5 and C 6 sugars to fermentation products, the method comprising: fermenting a C 6 -rich sugar feed to a first fermentation product; fermenting a C 5 -rich sugar feed to a second fermentation product; removing microorganism cells from the first fermentor, to maintain a cell concentration within a selected range; conveying microorganism cells to a second fermentor; and removing microorganism cells from the second fermentor, to maintain a microorganism cell concentration that is greater than that in the C 6 -rich fermentor.
摘要翻译：本发明提供了纤维素和半纤维素糖的优化发酵。利用微生物，代谢中间体和营养物质的再利用和再循环，将生物质衍生的半纤维素和纤维素糖独立调节并分别发酵。条件糖可以在单独的容器中发酵，其中来自葡萄糖发酵的过量细胞与来自溶剂回收的提余液一起被输送到半纤维素糖发酵，以提高生产率和产品产量。一些变化提供了将C 5和C 6糖发酵成发酵产物的方法，所述方法包括：发酵富含C 6的糖进料到第一发酵产物; 将第C 5高丰度糖原料发酵成第二发酵产物; 从第一发酵罐中除去微生物细胞，以将细胞浓度保持在选定范围内; 将微生物细胞输送至第二发酵罐; 并从第二发酵罐中除去微生物细胞，以保持比富含Cys6的发酵罐更高的微生物细胞浓度。

4. 发明申请

WO2017050291A1 USE OF SERINE PROTEASES FOR IMPROVING ETHANOL YIELD 审中-公开
标题翻译：使用丝氨酸蛋白酶改善乙醇含量
公开(公告)号：WO2017050291A1
公开(公告)日：2017-03-30
申请号：PCT/CN2016/100090
申请日：2016-09-26
申请人： NOVOZYMES A/S , GUO, Xinyan
发明人： GUO, Xinyan , KREEL, Nathaniel Edward , JUMP, Joseph , HOOSS, Melissa C. , ROBERTS, Madison , VIDAL, Bernardo, Jr. , JENSEN, Kenneth , FRISNER, Henrik , HOFF, Tine , LIU, Ye , TANG, Lan
IPC分类号： C12N9/54 , C12P1/00 , C12P7/06 , C12N9/30 , C12N9/34 , C12N9/40 , C12N15/63 , C12N9/48 , C12N9/58 , C12N9/50
CPC分类号： C12N9/48 , C12N9/50 , C12N9/54 , C12N9/58 , C12N15/63 , C12P7/06 , C12P2203/00 , C12Y302/01001 , C12Y304/14 , C12Y304/21 , Y02E50/17
摘要： S53 proteases and the use of S53 protease in processes for converting starch to ethanol are provided.
摘要翻译：提供S53蛋白酶和在淀粉转化为乙醇的方法中使用S53蛋白酶。

5. 发明申请

WO2016201031A1 HYDROTHERMAL-MECHANICAL TREATMENT OF LIGNOCELLULOSIC BIOMASS FOR PRODUCTION OF FERMENTATION PRODUCTS 审中-公开
标题翻译：生物发酵生物质的水热机械处理
公开(公告)号：WO2016201031A1
公开(公告)日：2016-12-15
申请号：PCT/US2016/036571
申请日：2016-06-09
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： RETSINA, Theodora , BOUSQUET, Jean-Pierre
IPC分类号： C12P7/06 , C12P7/16 , C12P7/18 , C12P7/46 , C12P7/56 , C08L97/02 , C12N1/00 , C12N9/00
CPC分类号： C12P19/14 , C12P7/10 , C12P7/14 , C12P7/16 , C12P7/18 , C12P7/46 , C12P7/56 , C12P19/02 , C12P2203/00 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： A simple process for converting lignocellulosic biomass into fermentation products is disclosed. Biomass may be subjected to a steam or hot-water soak to dissolve hemicelluloses. This step is followed by mechanical refining, such as in a hot-blow refiner, of the cellulose-rich (and lignin-rich) solids. The refined solids are then enzymatically hydrolyzed to generate sugars. Certain embodiments provide a process for producing ethanol, comprising: digesting a cellulosic biomass feedstock with steam or hot water to produce cellulose-rich solids, hemicellulose oligomers, and lignin; conveying the digested stream through a blow-line refiner; separating a vapor from the refined stream; introducing the refined stream to an enzymatic hydrolysis unit to produce sugars; fermenting the sugars to produce ethanol in dilute solution; and concentrating the dilute solution to produce an ethanol product. Enzymes and microorganisms may be introduced at various points in the process. The invention may be applied to any other fermentation product.
摘要翻译：公开了将木质纤维素生物质转化为发酵产物的简单方法。生物质可以经受蒸汽或热水浸泡以溶解半纤维素。该步骤之后是富含纤维素（和富含木质素的）固体的机械精炼，例如在热吹炼精炼机中。然后将精制的固体酶促水解以产生糖。某些实施方案提供了一种生产乙醇的方法，包括：用蒸汽或热水消化纤维素生物质原料以产生富含纤维素的固体，半纤维素低聚物和木质素; 通过吹塑精炼机输送消化液流; 从精炼流中分离蒸气; 将精制的流引入酶水解单元以产生糖; 发酵糖在稀溶液中生产乙醇; 并浓缩稀释溶液以产生乙醇产物。可以在该过程的各个点引入酶和微生物。本发明可以应用于任何其它发酵产物。

6. 发明申请

WO2016164389A1 PROCESS FOR BIOFUEL AND BIOCHEMICAL PRODUCTION, CULTURE MEDIUM AND BIOFUEL AND BIOCHEMICAL PRODUCED 审中-公开
标题翻译：生物和生物化学生产过程，培养基和生物化学和生物化学生产
公开(公告)号：WO2016164389A1
公开(公告)日：2016-10-13
申请号：PCT/US2016/026110
申请日：2016-04-06
申请人： API INTELLECTUAL PROPERTY HOLDINGS, LLC
发明人： GRASSI, Maria Carolina , PEREIRA, Gonçalo , RETSINA, Theodora
IPC分类号： C12P7/06 , C12P7/10 , C12P7/16 , C12P7/28 , C12P19/02 , D21C3/00 , D21H11/02
CPC分类号： C12P7/28 , C12P7/065 , C12P7/10 , C12P7/16 , C12P2203/00 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to a process and a culture medium for biofuel and biochemical production by fermentation of lignocellulose biomass. The process describes the use of a solid-liquid mixed blend formed by solid fiber pulp, hydrolysate, and a polypeptide complex. The solid fiber pulp is partially degraded by the polypeptide complex allowing microbe immobilization and, at the same time, releasing substances that affect Clostridium quorum sensing pathways. The present process and culture medium allow the improvement of biofuels and biochemical production, as butanol and acetone in an industrial scale.
摘要翻译：本发明涉及通过木质纤维素生物质发酵生物燃料和生物化学生产的方法和培养基。该方法描述了由固体纤维纸浆，水解产物和多肽复合物形成的固液混合共混物的用途。固体纤维纸浆被多肽复合物部分降解，允许微生物固定化，同时释放影响梭菌感染途径的物质。本工艺和培养基允许改进生物燃料和生化生产，如工业规模的丁醇和丙酮。

7. 发明申请

WO2016145529A1 PROCESS FOR TREATING LIGNOCELLULOSIC FEEDSTOCK COMPRISING WET OXIDATION 审中-公开
标题翻译：用于处理包含湿气氧化的液体饲料的方法
公开(公告)号：WO2016145529A1
公开(公告)日：2016-09-22
申请号：PCT/CA2016/050290
申请日：2016-03-16
申请人： IOGEN CORPORATION
发明人： FOODY, Patrick J. , FOODY, Brian , DECHMAN, John
IPC分类号： C12P7/10 , C01C1/24 , C01D5/00 , C05C3/00 , C05D1/02 , C08H8/00 , C10L1/02 , C12P1/00 , C12P19/02 , C12P19/14 , C12P7/02
CPC分类号： C01C1/24 , C01C1/164 , C01D5/00 , C01D5/04 , C05C3/00 , C05D1/02 , C05F17/0045 , C07G1/00 , C08H8/00 , C10L1/02 , C12F3/00 , C12P7/10 , C12P7/16 , C12P19/02 , C12P19/14 , C12P2201/00 , C12P2203/00 , Y02E50/10 , Y02E50/16 , Y02E50/343 , Y02P20/145 , Y02W30/43 , Y02W30/47
摘要： A process for producing one or more products from a lignocellulosic feedstock comprising treating the lignocellulosic feedstock to produce sugar in one or more stages comprising addition of acid, base or a combination thereof and introduction of heat. The addition of the acid, base, or a combination thereof produces a salt. The sugar is fermented to produce a fermentation product and the fermentation product is recovered. A stream comprising the salt is treated by wet oxidation with the introduction of heat. A stream comprising salt resulting from the wet oxidation is provided for use as a salt product or a process chemical for introduction within the process. Heat from the wet oxidation may be supplied to any stage of the process in which heat is introduced.
摘要翻译：一种用于从木质纤维素原料生产一种或多种产物的方法，包括在一个或多个阶段中处理木质纤维素原料以制备糖，包括加入酸，碱或其组合并引入热。加入酸，碱或其组合产生盐。糖被发酵以产生发酵产物并回收发酵产物。包含盐的料流通过加热进行湿式氧化处理。提供包含由湿氧化产生的盐的料流用作在该方法中引入的盐产物或加工化学品。来自湿氧化的热可以被供应到引入热的过程的任何阶段。

8. 发明申请

WO2016142550A1 A PROCESS FOR PRODUCING A HYDROLYZED MIXTURE FROM A PRE-TREATED LIGNO-CELLULOSIC SLURRY COMPRISING A SLURRY LIQUID AND SLURRY SOLIDS 审中-公开
标题翻译：从包含浆液和浆液的预处理的细胞浆液中生产水解混合物的方法
公开(公告)号：WO2016142550A1
公开(公告)日：2016-09-15
申请号：PCT/EP2016/055424
申请日：2016-03-14
申请人： BETA RENEWABLES S.P.A. , NOVOZYMES A/S
发明人： RIVA, Daniele , PURROTTI, Micol , MONTECCHI, Donatello , CRIPPA, Tommaso , OTTONELLO, Piero , PREFUMO, Chiara , FERRERO, Simone , PARAVISI, Stefano , BELFRAGE, Johan , FRICKMANN, Jesper
IPC分类号： C12P19/14 , C08H8/00 , C08L97/02 , C13K1/00 , C13K13/00
CPC分类号： C12P19/14 , C08H8/00 , C12P2201/00 , C12P2203/00 , C13K1/02 , C13K13/00
摘要： It is disclosed a process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids. In the disclosed process, at least a portion of a cellulase from an enzyme cocktail having an initial cellulase activity is adsorbed onto the slurry solids of the pre-treated ligno-cellulosic biomass slurry to create an adsorbed cellulase, wherein the adsorbed cellulase has an adsorbed cellulase activity which is greater than 45% of the initial cellulase activity. The pre-treated ligno-cellulosic slurry with adsorbed enzymes is subjected to enzymatic hydrolysis to produce the hydrolyzed mixture. The enzymatic hydrolysis may comprise more than one hydrolysis step, which may be continuous or batch.
摘要翻译：公开了一种从预处理的木质纤维素浆料中生产水解混合物的方法，其包括浆液和浆料固体。在所公开的方法中，将来自具有初始纤维素酶活性的酶混合物的纤维素酶的至少一部分吸附到预处理的木质纤维素生物质浆料的浆料固体上以产生吸附的纤维素酶，其中吸附的纤维素酶具有吸附纤维素酶活性大于初始纤维素酶活性的45％。将具有吸附的酶的经预处理的木质纤维素浆料进行酶水解以产生水解的混合物。酶水解可以包含多于一个的水解步骤，其可以是连续的或分批的。

9. 发明申请

WO2016123043A1 PROCESS OF FERMENTING A LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：发展细菌生物量的过程
公开(公告)号：WO2016123043A1
公开(公告)日：2016-08-04
申请号：PCT/US2016/014805
申请日：2016-01-26
申请人： AUBURN UNIVERSITY
发明人： TU, Maobing , MALINOWSKI, Rui
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , C12P2201/00 , C12P2203/00 , Y02E50/16
摘要： The present disclosure provides methods of fermenting a lignocellulosic biomass, wherein a nucleophile is added to deactivate carbonyl-containing fermentation inhibitors in hydrolysate formulated by pretreating the lignocellulosic biomass. The disclosure also provides methods of fermenting a lignocellulosic biomass, wherein a nucleophile is added to deactivate carbonyl-containing fermentation inhibitors in a slurry formulated by pretreating the lignocellulosic biomass.
摘要翻译：本公开提供了发酵木质纤维素生物质的方法，其中加入亲核试剂以使通过预处理木质纤维素生物质配制的水解产物中含羰基的发酵抑制剂失活。本公开还提供了发酵木质纤维素生物质的方法，其中加入亲核试剂以通过预处理木质纤维素生物质配制的浆料中使含羰基的发酵抑制剂失活。

10. 发明申请

WO2016121100A1 グルコースを主成分とする糖類の製造方法审中-公开
标题翻译：用于生产含糖葡萄糖作为主要成分的方法
公开(公告)号：WO2016121100A1
公开(公告)日：2016-08-04
申请号：PCT/JP2015/052683
申请日：2015-01-30
申请人：日揮株式会社
发明人：種田　大介
IPC分类号： C12P19/14 , C13K1/02
CPC分类号： C12P19/14 , C12P19/02 , C12P2201/00 , C12P2203/00 , C13K1/02
摘要：　安価に、酵素がリグニンに非生産的に吸着するのを抑制することができ、それによってグルコースを主成分とする糖類を効率よく製造する方法を提供する。すなわち、本発明の糖類の製造方法は、水酸化ナトリウム水溶液または水酸化カルシウム水溶液に、動物性タンパク質および植物性タンパク質の少なくともいずれか一方を添加して反応させ、水溶性タンパク質を調製する第１の工程と、バイオマスを含むスラリーに、前記水溶性タンパク質を添加する第２の工程と、前記スラリーに前記水溶性タンパク質を添加すると同時に、又は前記水溶性タンパク質を添加した後に、前記スラリーに分解酵素を添加し、前記バイオマスに含まれるセルロースおよびヘミセルロースの少なくともいずれか一方を前記分解酵素で分解して、グルコースを主成分とする糖類を製造する第３の工程とを含む。
摘要翻译：提供了可以以低成本防止酶对木质素的非生产性吸收的方法，因此能够以高效率生产含有葡萄糖作为主要成分的糖。也就是说，根据本发明的糖的制造方法包括：向氢氧化钠水溶液或氢氧化钙水溶液中添加动物蛋白质和/或植物蛋白质以引起其间的反应的第一步骤，由此制备水溶性蛋白质; 将水溶性蛋白质添加到含有生物质的浆料中的第二步骤; 以及第三步骤，在向所述浆料中添加水溶性蛋白质的同时添加消化酶，以使消化酶分解生物质中所含的纤维素和/或半纤维素，从而产生含糖的葡萄糖作为主要组成部分。

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